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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57PotassiumBy similarity1
Metal bindingi120PotassiumBy similarity1
Binding sitei151NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi154PotassiumBy similarity1
Binding sitei300NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei414NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi385 – 389ADPBy similarity5
Nucleotide bindingi404 – 413ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciHPY:HP1363-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:HP_1363
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001190441 – 466Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST466

Proteomic databases

PaxDbiP56176
PRIDEiP56176

Interactioni

Protein-protein interaction databases

STRINGi85962.HP1363

Structurei

Secondary structure

1466
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP56176
SMRiP56176
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP56176

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 207YjeF N-terminalAdd BLAST200
Domaini215 – 466YjeF C-terminalAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 207NAD(P)H-hydrate epimeraseBy similarityAdd BLAST207
Regioni56 – 60NAD(P)HX (for epimerase activity)By similarity5
Regioni124 – 130NAD(P)HX (for epimerase activity)By similarity7
Regioni215 – 466ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST252
Regioni342 – 348NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4105DR1 Bacteria
COG0062 LUCA
COG0063 LUCA
KOiK17758
K17759
OMAiKSPMCQL

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

P56176-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSVYEKVNA LDKRAIEELF LSEDILMENA AMALERAVLQ NASLGAKVII
60 70 80 90 100
LCGSGDNGGD GYALARRLVG RFKTLVFEMK LAKSPMCQLQ QERAKKAGVV
110 120 130 140 150
IKAYEENALN QNLECDVLID CVIGSHFKGK LEPFLNFESL SQKARFKIAC
160 170 180 190 200
DIPSGIDSKG RVDKGAFKAD LTISMGAIKS CLLSDRAKDY VGELKVGHLG
210 220 230 240 250
VFNQIYEIPT DTFLLEKSDL KLPLRDRKNA HKGDYGHAHV LLGKHSGAGL
260 270 280 290 300
LSALSALSFG SGVVSVQALE CEITSNNKPL ELVFCENFPN LLSAFALGMG
310 320 330 340 350
LENIPKDFNK WLELAPCVLD AGVFYHKEVL QALEKEVILT PHPKEFLSLL
360 370 380 390 400
KLVGINISML ELLDNKLEIA RDFSQKYPKV VLLLKGANTL IAHQGQVFIN
410 420 430 440 450
ILGSVALAKA GSGDVLAGLI LSLLSQNYTP LDAAINASSA HALASLEFKN
460
NYALTPLDLI EKIKQL
Length:466
Mass (Da):50,737
Last modified:November 1, 1997 - v1
Checksum:i52125FE79BA8F056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD08403.1
PIRiC64690
RefSeqiNP_208155.1, NC_000915.1
WP_000954017.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD08403; AAD08403; HP_1363
GeneIDi900323
KEGGiheo:C694_07035
hpy:HP1363
PATRICifig|85962.47.peg.1460

Similar proteinsi

Entry informationi

Entry nameiNNR_HELPY
AccessioniPrimary (citable) accession number: P56176
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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