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UniProtKB - P56176 (NNR_HELPY)
Protein
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Gene
nnr
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).
By similarityCatalytic activityi
Cofactori
K+By similarityNote: Binds 1 potassium ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 57 | PotassiumBy similarity | 1 | |
Metal bindingi | 120 | PotassiumBy similarity | 1 | |
Binding sitei | 151 | NADPHX 1; for epimerase activityBy similarity | 1 | |
Metal bindingi | 154 | PotassiumBy similarity | 1 | |
Binding sitei | 300 | NADPHX 2; for dehydratase activity; via amide nitrogenBy similarity | 1 | |
Binding sitei | 414 | NADPHX 2; for dehydratase activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 385 – 389 | ADPBy similarity | 5 | |
Nucleotide bindingi | 404 – 413 | ADPBy similarity | 10 |
GO - Molecular functioni
- ADP-dependent NAD(P)H-hydrate dehydratase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- NADHX epimerase activity Source: GO_Central
- NADPHX epimerase activity Source: GO_Central
GO - Biological processi
- metabolite repair Source: GO_Central
- nicotinamide nucleotide metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme |
Ligand | ATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional NAD(P)H-hydrate repair enzyme NnrAlternative name(s): Nicotinamide nucleotide repair protein Including the following 2 domains: |
Gene namesi | Name:nnr Ordered Locus Names:HP_1363 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119044 | 1 – 466 | Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST | 466 |
Proteomic databases
PaxDbi | P56176 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P56176 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P56176 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 207 | YjeF N-terminalAdd BLAST | 200 | |
Domaini | 215 – 466 | YjeF C-terminalAdd BLAST | 252 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 207 | NAD(P)H-hydrate epimeraseBy similarityAdd BLAST | 207 | |
Regioni | 56 – 60 | NADPHX 1; for epimerase activityBy similarity | 5 | |
Regioni | 124 – 130 | NADPHX 1; for epimerase activityBy similarity | 7 | |
Regioni | 215 – 466 | ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST | 252 | |
Regioni | 342 – 348 | NADPHX 2; for dehydratase activityBy similarity | 7 |
Sequence similaritiesi
In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated
Phylogenomic databases
eggNOGi | COG0062, Bacteria COG0063, Bacteria |
OMAi | ALECEIT |
PhylomeDBi | P56176 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit 3.40.50.10260, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit MF_01966, NADHX_epimerase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR030677, Nnr IPR029056, Ribokinase-like IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit PF03853, YjeF_N, 1 hit |
PIRSFi | PIRSF017184, Nnr, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit SSF64153, SSF64153, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit TIGR00197, yjeF_nterm, 1 hit |
PROSITEi | View protein in PROSITE PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit PS51385, YJEF_N, 1 hit |
i Sequence
Sequence statusi: Complete.
P56176-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLSVYEKVNA LDKRAIEELF LSEDILMENA AMALERAVLQ NASLGAKVII
60 70 80 90 100
LCGSGDNGGD GYALARRLVG RFKTLVFEMK LAKSPMCQLQ QERAKKAGVV
110 120 130 140 150
IKAYEENALN QNLECDVLID CVIGSHFKGK LEPFLNFESL SQKARFKIAC
160 170 180 190 200
DIPSGIDSKG RVDKGAFKAD LTISMGAIKS CLLSDRAKDY VGELKVGHLG
210 220 230 240 250
VFNQIYEIPT DTFLLEKSDL KLPLRDRKNA HKGDYGHAHV LLGKHSGAGL
260 270 280 290 300
LSALSALSFG SGVVSVQALE CEITSNNKPL ELVFCENFPN LLSAFALGMG
310 320 330 340 350
LENIPKDFNK WLELAPCVLD AGVFYHKEVL QALEKEVILT PHPKEFLSLL
360 370 380 390 400
KLVGINISML ELLDNKLEIA RDFSQKYPKV VLLLKGANTL IAHQGQVFIN
410 420 430 440 450
ILGSVALAKA GSGDVLAGLI LSLLSQNYTP LDAAINASSA HALASLEFKN
460
NYALTPLDLI EKIKQL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08403.1 |
PIRi | C64690 |
RefSeqi | NP_208155.1, NC_000915.1 WP_000954017.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08403; AAD08403; HP_1363 |
KEGGi | hpy:HP_1363 |
PATRICi | fig|85962.47.peg.1460 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08403.1 |
PIRi | C64690 |
RefSeqi | NP_208155.1, NC_000915.1 WP_000954017.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3K5W | X-ray | 2.60 | A | 2-466 | [»] | |
SMRi | P56176 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 85962.C694_07035 |
Proteomic databases
PaxDbi | P56176 |
Genome annotation databases
EnsemblBacteriai | AAD08403; AAD08403; HP_1363 |
KEGGi | hpy:HP_1363 |
PATRICi | fig|85962.47.peg.1460 |
Phylogenomic databases
eggNOGi | COG0062, Bacteria COG0063, Bacteria |
OMAi | ALECEIT |
PhylomeDBi | P56176 |
Miscellaneous databases
EvolutionaryTracei | P56176 |
Family and domain databases
CDDi | cd01171, YXKO-related, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit 3.40.50.10260, 1 hit |
HAMAPi | MF_01965, NADHX_dehydratase, 1 hit MF_01966, NADHX_epimerase, 1 hit |
InterProi | View protein in InterPro IPR017953, Carbohydrate_kinase_pred_CS IPR000631, CARKD IPR030677, Nnr IPR029056, Ribokinase-like IPR004443, YjeF_N_dom IPR036652, YjeF_N_dom_sf |
Pfami | View protein in Pfam PF01256, Carb_kinase, 1 hit PF03853, YjeF_N, 1 hit |
PIRSFi | PIRSF017184, Nnr, 1 hit |
SUPFAMi | SSF53613, SSF53613, 1 hit SSF64153, SSF64153, 1 hit |
TIGRFAMsi | TIGR00196, yjeF_cterm, 1 hit TIGR00197, yjeF_nterm, 1 hit |
PROSITEi | View protein in PROSITE PS01050, YJEF_C_2, 1 hit PS51383, YJEF_C_3, 1 hit PS51385, YJEF_N, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNR_HELPY | |
Accessioni | P56176Primary (citable) accession number: P56176 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families