UniProtKB - P56079 (CDS_DROME)
Protein
Phosphatidate cytidylyltransferase, photoreceptor-specific
Gene
Cds
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an important precursor for the synthesis of phosphatidylinositol (PtdIns) and phosphatidylglycerol (PG) (PubMed:7816135, PubMed:24603715). Required for the regeneration of the signaling molecule phosphatidylinositol 4,5-bisphosphate (PtdInsP2) from PA and maintenance of its steady supply during signaling thus playing an essential role during phospholipase C-mediated transduction (PubMed:7816135). In the salivary glands and possibly other adipose tissues, function is essential for regulating cell growth and neutral lipid storage by coordinating PtdIns metabolism and insulin pathway activity (PubMed:24603715). Acts by positively regulating activity of the insulin pathway through synthesis of PtdIns, and in turn the insulin pathway up-regulates the synthesis of CdsA (PubMed:24603715). This CdsA-insulin positive feedback loop may be one of the mechanisms for coordinating cell growth and fat storage; switching to fat storage when cells reach homeostasis or converting from growth to fat storage under nutrient-poor conditions (PubMed:24603715). Required for spermatid individualization by regulating lipid compositions and lipid-mediated signaling during spermatogenesis (PubMed:26791243).3 Publications
Catalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H+ = a CDP-1,2-diacyl-sn-glycerol + diphosphate2 Publications1 PublicationEC:2.7.7.412 Publications1 Publication
: CDP-diacylglycerol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.2 Publications1 PublicationProteins known to be involved in the 3 steps of the subpathway in this organism are:
- no protein annotated in this organism
- no protein annotated in this organism
- Phosphatidate cytidylyltransferase, mitochondrial (CG33331), Phosphatidate cytidylyltransferase (CdsA-RA), Phosphatidate cytidylyltransferase (CdsA-RA), Phosphatidate cytidylyltransferase (Cds), Phosphatidate cytidylyltransferase, photoreceptor-specific (Cds), Phosphatidate cytidylyltransferase (CdsA-RA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- phosphatidate cytidylyltransferase activity Source: FlyBase
GO - Biological processi
- CDP-diacylglycerol biosynthetic process Source: FlyBase
- lipid biosynthetic process Source: FlyBase
- negative regulation of triglyceride biosynthetic process Source: FlyBase
- phototransduction Source: FlyBase
- positive regulation of phosphatidylinositol biosynthetic process Source: FlyBase
- regulation of lipid storage Source: FlyBase
- rhodopsin mediated signaling pathway Source: FlyBase
- sperm individualization Source: FlyBase
- terminal branching, open tracheal system Source: FlyBase
- thermotaxis Source: FlyBase
- visual perception Source: UniProtKB-KW
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Sensory transduction, Vision |
Enzyme and pathway databases
Reactomei | R-DME-1483148, Synthesis of PG R-DME-1483226, Synthesis of PI |
UniPathwayi | UPA00557;UER00614 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidate cytidylyltransferase, photoreceptor-specific (EC:2.7.7.412 Publications1 Publication)Alternative name(s): CDP-DAG synthase1 Publication CDP-DG synthase CDP-diacylglycerol synthaseImported Short name: CDS CDP-diglyceride pyrophosphorylase CDP-diglyceride synthase CTP:phosphatidate cytidylyltransferase |
Gene namesi | ORF Names:CG7962Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0010350, Cds |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 88 – 108 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 147 – 167 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 180 – 200 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 203 – 223 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 227 – 247 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 276 – 296 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 350 – 370 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Lethal during the late embryonic to early larval stages (PubMed:24603715). Hypomorphic mutants are viable but male adults are sterile, whereas female adults remain fertile (PubMed:26791243). Impairs spermatid individualization showing abnormal mitochondria morphology and defects in lipid compositions and lipid-mediated signaling (PubMed:26791243). RNAi-mediated knockdown results in low insulin pathway activity and defective cell growth in certain tissues (PubMed:24603715). Various non-adipose tissues display excessive fat accumulation such as the salivary glands, proventriculus, hind gut, Malpighian tubules and trachea (PubMed:24603715). In addition gross reduction in cell size results in smaller salivary glands, imaginal disks and brains compared to controls (PubMed:24603715). RNAi-mediated knockdown in both the salivary glands and fat body, results in decreased levels of phosphatidylinositol (PtdIns) in the whole larvae and in the salivary glands (PubMed:24603715). Whole larvae also display decreased levels of phosphatidylglycerol (PG) and increased levels of phosphatidic acid (PA) (PubMed:24603715). Larvae display low insulin pathway activity with reduced levels of phosphatidylinositol 4,5-bisphosphate (PtdInsP2) and phosphatidylinositol 3,4,5-trisphosphate (Ptdinsp3), and decreased levels of 'S-505' phosphorylated Akt1 (PubMed:24603715). RNAi-mediated knockdown specifically in the fat body, results in a decrease in PtdIns levels but there is no effect on the growth and neutral lipid storage in the fat body (PubMed:24603715). This is likely due to unknown compensatory mechanisms as there is an increase in the levels of diphosphate diacylglycerol and phosphatidylethanolamine in the fat body (PubMed:24603715).2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000090720 | 1 – 447 | Phosphatidate cytidylyltransferase, photoreceptor-specificAdd BLAST | 447 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 30 | Phosphoserine1 Publication | 1 | |
Modified residuei | 34 | Phosphoserine1 Publication | 1 | |
Modified residuei | 35 | Phosphoserine1 Publication | 1 | |
Modified residuei | 40 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P56079 |
PRIDEi | P56079 |
PTM databases
iPTMneti | P56079 |
Expressioni
Tissue specificityi
Retina. Localized to the photoreceptor neurons, both in the compound eyes and ocelli.
Developmental stagei
In 3rd instar larvae, expressed in various tissues including the brain, salivary glands, fat body, proventriculus, hind gut, muscle, Malpighian tubules and tracheal tubes. Relatively low levels of expression in the brain compared to expression levels in the salivary gland, fat body and gut.1 Publication
Gene expression databases
Bgeei | FBgn0010350, Expressed in brain and 19 other tissues |
ExpressionAtlasi | P56079, baseline and differential |
Genevisiblei | P56079, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 68739, 20 interactors |
DIPi | DIP-23447N |
IntActi | P56079, 3 interactors |
STRINGi | 7227.FBpp0076411 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CDS family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1440, Eukaryota |
HOGENOMi | CLU_023471_0_1_1 |
InParanoidi | P56079 |
OMAi | VVQAHFI |
OrthoDBi | 1072976at2759 |
PhylomeDBi | P56079 |
Family and domain databases
InterProi | View protein in InterPro IPR000374, PC_trans IPR016720, PC_Trfase_euk |
PANTHERi | PTHR13773, PTHR13773, 1 hit |
PIRSFi | PIRSF018269, PC_trans_euk, 1 hit |
PROSITEi | View protein in PROSITE PS01315, CDS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P56079-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEVRRRKGE DEPLEDTAIS GSDAANKRNS AADSSDHVDS EEEKIPEEKF
60 70 80 90 100
VDELAKNLPQ GTDKTPEILD SALKDLPDRW KNWVIRGIFT WIMICGFALI
110 120 130 140 150
IYGGPLALMI TTLLVQVKCF QEIISIGYQV YRIHGLPWFR SLSWYFLLTS
160 170 180 190 200
NYFFYGENLV DYFGVVINRV EYLKFLVTYH RFLSFALYII GFVWFVLSLV
210 220 230 240 250
KKYYIKQFSL FAWTHVSLLI VVTQSYLIIQ NIFEGLIWFI VPVSMIVCND
260 270 280 290 300
VMAYVFGFFF GRTPLIKLSP KKTWEGFIGG GFATVLFGIL FSYVLCNYQY
310 320 330 340 350
FICPIQYSEE QGRMTMSCVP SYLFTPQEYS LKLFGIGKTL NLYPFIWHSI
360 370 380 390 400
SLSLFSSIIG PFGGFFASGF KRAFKIKDFG DMIPGHGGIM DRFDCQFLMA
410 420 430 440
TFVNVYISSF IRTPSPAKLL TQIYNLKPDQ QYQIYQSLKD NLGDMLT
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2JC55 | X2JC55_DROME | Phosphatidate cytidylyltransferase | Cds CDS, cds, CdsA, cdsA, Dmel\CG7962 | 447 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 444 | D → H no nucleotide entry (PubMed:7816135).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF50483.1 |
RefSeqi | NP_001286976.1, NM_001300047.1 NP_524661.1, NM_079922.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0076688; FBpp0076411; FBgn0010350 FBtr0346546; FBpp0312164; FBgn0010350 |
GeneIDi | 43950 |
KEGGi | dme:Dmel_CG7962 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF50483.1 |
RefSeqi | NP_001286976.1, NM_001300047.1 NP_524661.1, NM_079922.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 68739, 20 interactors |
DIPi | DIP-23447N |
IntActi | P56079, 3 interactors |
STRINGi | 7227.FBpp0076411 |
PTM databases
iPTMneti | P56079 |
Proteomic databases
PaxDbi | P56079 |
PRIDEi | P56079 |
Genome annotation databases
EnsemblMetazoai | FBtr0076688; FBpp0076411; FBgn0010350 FBtr0346546; FBpp0312164; FBgn0010350 |
GeneIDi | 43950 |
KEGGi | dme:Dmel_CG7962 |
Organism-specific databases
CTDi | 43950 |
FlyBasei | FBgn0010350, Cds |
Phylogenomic databases
eggNOGi | KOG1440, Eukaryota |
HOGENOMi | CLU_023471_0_1_1 |
InParanoidi | P56079 |
OMAi | VVQAHFI |
OrthoDBi | 1072976at2759 |
PhylomeDBi | P56079 |
Enzyme and pathway databases
UniPathwayi | UPA00557;UER00614 |
Reactomei | R-DME-1483148, Synthesis of PG R-DME-1483226, Synthesis of PI |
Miscellaneous databases
BioGRID-ORCSi | 43950, 0 hits in 3 CRISPR screens |
ChiTaRSi | CdsA, fly |
GenomeRNAii | 43950 |
PROi | PR:P56079 |
Gene expression databases
Bgeei | FBgn0010350, Expressed in brain and 19 other tissues |
ExpressionAtlasi | P56079, baseline and differential |
Genevisiblei | P56079, DM |
Family and domain databases
InterProi | View protein in InterPro IPR000374, PC_trans IPR016720, PC_Trfase_euk |
PANTHERi | PTHR13773, PTHR13773, 1 hit |
PIRSFi | PIRSF018269, PC_trans_euk, 1 hit |
PROSITEi | View protein in PROSITE PS01315, CDS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDS_DROME | |
Accessioni | P56079Primary (citable) accession number: P56079 Secondary accession number(s): Q9VSE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families