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UniProtKB - P55859 (PNPH_BOVIN)
Protein
Purine nucleoside phosphorylase
Gene
PNP
Organism
Bos taurus (Bovine)
Status
Functioni
Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate (By similarity).
Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine (By similarity).
By similarityCatalytic activityi
: purine nucleoside salvage Pathwayi
This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | PhosphateCombined sources1 Publication | 1 | |
Binding sitei | 64 | PhosphateCombined sources1 Publication | 1 | |
Binding sitei | 88 | Purine nucleosideCombined sources1 Publication | 1 | |
Binding sitei | 116 | Phosphate; via amide nitrogenCombined sources1 Publication | 1 | |
Binding sitei | 201 | Purine nucleosideCombined sources1 Publication1 Publication | 1 | |
Binding sitei | 219 | Purine nucleosideCombined sources1 Publication | 1 | |
Binding sitei | 220 | PhosphateCombined sources1 Publication | 1 | |
Binding sitei | 243 | Purine nucleosideCombined sources1 Publication1 Publication | 1 | |
Sitei | 243 | Important for substrate specificityBy similarity | 1 | |
Binding sitei | 257 | Purine nucleosideCombined sources1 Publication | 1 |
GO - Molecular functioni
- guanosine phosphorylase activity Source: RHEA
- purine-nucleoside phosphorylase activity Source: UniProtKB
GO - Biological processi
- purine ribonucleoside salvage Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Enzyme and pathway databases
BRENDAi | 2.4.2.1, 908 |
SABIO-RKi | P55859 |
UniPathwayi | UPA00606 |
Names & Taxonomyi
Protein namesi | Recommended name: Purine nucleoside phosphorylase (EC:2.4.2.1By similarity)Short name: PNP Alternative name(s): Inosine phosphorylase Inosine-guanosine phosphorylase |
Gene namesi | Name:PNP Synonyms:NP |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184535 | 1 – 289 | Purine nucleoside phosphorylaseAdd BLAST | 289 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 251 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P55859 |
PeptideAtlasi | P55859 |
PRIDEi | P55859 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000012317, Expressed in bone marrow and 98 other tissues |
Interactioni
Subunit structurei
Homotrimer.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016346 |
Chemistry databases
BindingDBi | P55859 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P55859 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55859 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 84 – 86 | Phosphate bindingCombined sources1 Publication | 3 |
Sequence similaritiesi
Belongs to the PNP/MTAP phosphorylase family.Curated
Phylogenomic databases
eggNOGi | KOG3984, Eukaryota |
HOGENOMi | CLU_054456_1_2_1 |
InParanoidi | P55859 |
OMAi | EGVYAQF |
TreeFami | TF300049 |
Family and domain databases
CDDi | cd09009, PNP-EcPNPII_like, 1 hit |
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR011270, Pur_Nuc_Pase_Ino/Guo-sp IPR011268, Purine_phosphorylase IPR018099, Purine_phosphorylase-2_CS |
PANTHERi | PTHR11904, PTHR11904, 1 hit |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
PIRSFi | PIRSF000477, PurNPase, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01700, PNPH, 1 hit TIGR01697, PNPH-PUNA-XAPA, 1 hit |
PROSITEi | View protein in PROSITE PS01240, PNP_MTAP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P55859-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANGYTYEDY QDTAKWLLSH TEQRPQVAVI CGSGLGGLVN KLTQAQTFDY
60 70 80 90 100
SEIPNFPEST VPGHAGRLVF GILNGRACVM MQGRFHMYEG YPFWKVTFPV
110 120 130 140 150
RVFRLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FSGENPLRGP
160 170 180 190 200
NEERFGVRFP AMSDAYDRDM RQKAHSTWKQ MGEQRELQEG TYVMLGGPNF
210 220 230 240 250
ETVAECRLLR NLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE
260 270 280
SQGKANHEEV LEAGKQAAQK LEQFVSLLMA SIPVSGHTG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | A → Q AA sequence (PubMed:7607309).Curated | 1 | |
Sequence conflicti | 25 | P → S in AAX46392 (PubMed:16305752).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021545 mRNA Translation: AAX46392.1 BC103291 mRNA Translation: AAI03292.2 |
PIRi | S66203 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT021545 mRNA Translation: AAX46392.1 BC103291 mRNA Translation: AAI03292.2 |
PIRi | S66203 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A9O | X-ray | 2.00 | A | 1-289 | [»] | |
1A9P | X-ray | 2.40 | A | 1-289 | [»] | |
1A9Q | X-ray | 2.00 | A | 1-282 | [»] | |
1A9R | X-ray | 2.00 | A | 1-282 | [»] | |
1A9S | X-ray | 2.00 | A | 1-289 | [»] | |
1A9T | X-ray | 2.00 | A | 1-284 | [»] | |
1B8N | X-ray | 2.00 | A | 1-284 | [»] | |
1B8O | X-ray | 1.50 | A | 1-284 | [»] | |
1FXU | X-ray | 2.20 | A | 1-289 | [»] | |
1LV8 | X-ray | 2.30 | A/B/C/D/E/F | 1-289 | [»] | |
1LVU | X-ray | 2.05 | A/B/C/D/E/F | 1-289 | [»] | |
1PBN | X-ray | 2.00 | A | 1-289 | [»] | |
1V48 | X-ray | 2.20 | A | 1-289 | [»] | |
1VFN | X-ray | 2.15 | A | 4-284 | [»] | |
2AI1 | X-ray | 2.00 | A | 1-289 | [»] | |
2AI2 | X-ray | 1.70 | A | 1-289 | [»] | |
2AI3 | X-ray | 1.70 | A | 1-289 | [»] | |
2QPL | X-ray | 2.10 | A | 3-284 | [»] | |
3FUC | X-ray | 1.45 | A/B/C | 1-284 | [»] | |
3PNP | X-ray | 1.60 | A | 1-289 | [»] | |
4PNP | X-ray | 1.80 | A | 1-289 | [»] | |
SMRi | P55859 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000016346 |
Chemistry databases
BindingDBi | P55859 |
ChEMBLi | CHEMBL2935 |
DrugCentrali | P55859 |
Proteomic databases
PaxDbi | P55859 |
PeptideAtlasi | P55859 |
PRIDEi | P55859 |
Phylogenomic databases
eggNOGi | KOG3984, Eukaryota |
HOGENOMi | CLU_054456_1_2_1 |
InParanoidi | P55859 |
OMAi | EGVYAQF |
TreeFami | TF300049 |
Enzyme and pathway databases
UniPathwayi | UPA00606 |
BRENDAi | 2.4.2.1, 908 |
SABIO-RKi | P55859 |
Miscellaneous databases
EvolutionaryTracei | P55859 |
Gene expression databases
Bgeei | ENSBTAG00000012317, Expressed in bone marrow and 98 other tissues |
Family and domain databases
CDDi | cd09009, PNP-EcPNPII_like, 1 hit |
Gene3Di | 3.40.50.1580, 1 hit |
InterProi | View protein in InterPro IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR011270, Pur_Nuc_Pase_Ino/Guo-sp IPR011268, Purine_phosphorylase IPR018099, Purine_phosphorylase-2_CS |
PANTHERi | PTHR11904, PTHR11904, 1 hit |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
PIRSFi | PIRSF000477, PurNPase, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01700, PNPH, 1 hit TIGR01697, PNPH-PUNA-XAPA, 1 hit |
PROSITEi | View protein in PROSITE PS01240, PNP_MTAP_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PNPH_BOVIN | |
Accessioni | P55859Primary (citable) accession number: P55859 Secondary accession number(s): Q3ZBH6, Q58DQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 5, 2008 | |
Last modified: | June 2, 2021 | |
This is version 150 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families