UniProtKB - P55345 (ANM2_HUMAN)
Protein arginine N-methyltransferase 2
PRMT2
Functioni
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
4 PublicationsCatalytic activityi
- L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + Nω,Nω-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.3191 Publication
Kineticsi
- KM=2.6 µM for AdoMet1 Publication
- KM=3.3 µM for H41 Publication
- Vmax=1.4 pmol/min/mg enzyme toward S-adenosyl-L-methionine (AdoMet)1 Publication
- Vmax=1.5 pmol/min/mg enzyme toward histone H41 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 112 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 121 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 145 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 168 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 197 | S-adenosyl-L-methionineBy similarity | 1 | |
Active sitei | 211 | By similarity | 1 | |
Active sitei | 220 | By similarity | 1 |
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- estrogen receptor binding Source: UniProtKB
- histone-arginine N-methyltransferase activity Source: UniProtKB
- histone methyltransferase activity Source: UniProtKB
- peroxisome proliferator activated receptor binding Source: UniProtKB
- progesterone receptor binding Source: UniProtKB
- protein-arginine N-methyltransferase activity Source: UniProtKB
- protein-arginine omega-N asymmetric methyltransferase activity Source: UniProtKB-EC
- protein-containing complex binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- retinoic acid receptor binding Source: UniProtKB
- thyroid hormone receptor binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- developmental cell growth Source: UniProtKB
- histone methylation Source: UniProtKB
- negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- protein methylation Source: ProtInc
- regulation of androgen receptor signaling pathway Source: UniProtKB
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.319, 2681 |
PathwayCommonsi | P55345 |
SignaLinki | P55345 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein arginine N-methyltransferase 2 (EC:2.1.1.3191 Publication)Alternative name(s): Histone-arginine N-methyltransferase PRMT2 |
Gene namesi | Name:PRMT2 Synonyms:HMT1, HRMT1L1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:5186, PRMT2 |
MIMi | 601961, gene |
neXtProti | NX_P55345 |
VEuPathDBi | HostDB:ENSG00000160310 |
Subcellular locationi
Nucleus
Note: Excluded from nucleolus.
Nucleus
Note: Excluded from nucleolus.
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 3275 |
OpenTargetsi | ENSG00000160310 |
PharmGKBi | PA29460 |
Miscellaneous databases
Pharosi | P55345, Tbio |
Genetic variation databases
BioMutai | PRMT2 |
DMDMi | 2499805 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212324 | 1 – 433 | Protein arginine N-methyltransferase 2Add BLAST | 433 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | Asymmetric dimethylarginineCombined sources | 1 | |
Modified residuei | 72 | Asymmetric dimethylarginineCombined sources | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | P55345 |
jPOSTi | P55345 |
MassIVEi | P55345 |
PaxDbi | P55345 |
PeptideAtlasi | P55345 |
PRIDEi | P55345 |
ProteomicsDBi | 56853 [P55345-1] 56854 [P55345-2] 56855 [P55345-3] 6261 6262 6263 |
PTM databases
iPTMneti | P55345 |
PhosphoSitePlusi | P55345 |
SwissPalmi | P55345 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000160310, Expressed in cortical plate and 241 other tissues |
ExpressionAtlasi | P55345, baseline and differential |
Genevisiblei | P55345, HS |
Organism-specific databases
HPAi | ENSG00000160310, Low tissue specificity |
Interactioni
Subunit structurei
Self-associates.
Interacts with RB1 and E2F1 (By similarity).
Interacts with NCOA6 coactivator.
Interacts (via SH3 domain) with PRMT8.
Interacts with AR.
Interacts with NFKBIA.
Interacts with ESR1, ESR2, PGR, PPARG, RARA, RXRA and THRB.
Interacts with HNRNPUL1.
By similarity6 PublicationsBinary interactionsi
P55345
GO - Molecular functioni
- androgen receptor binding Source: UniProtKB
- estrogen receptor binding Source: UniProtKB
- peroxisome proliferator activated receptor binding Source: UniProtKB
- progesterone receptor binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- retinoic acid receptor binding Source: UniProtKB
- thyroid hormone receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 109511, 69 interactors |
CORUMi | P55345 |
IntActi | P55345, 54 interactors |
MINTi | P55345 |
STRINGi | 9606.ENSP00000380759 |
Miscellaneous databases
RNActi | P55345, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P55345 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55345 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 89 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 99 – 432 | SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST | 334 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 277 | Interaction with ESR1Add BLAST | 277 | |
Regioni | 1 – 20 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 83 – 207 | Interaction with RB1By similarityAdd BLAST | 125 | |
Regioni | 133 – 275 | Interaction with ESR1Add BLAST | 143 |
Sequence similaritiesi
Keywords - Domaini
SH3 domainPhylogenomic databases
eggNOGi | KOG1499, Eukaryota |
GeneTreei | ENSGT00940000160683 |
HOGENOMi | CLU_017375_4_0_1 |
InParanoidi | P55345 |
OMAi | GTKSFPM |
PhylomeDBi | P55345 |
TreeFami | TF332196 |
Family and domain databases
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR007848, Small_mtfrase_dom |
PANTHERi | PTHR11006, PTHR11006, 1 hit |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit PS50002, SH3, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATSGDCPRS ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS
60 70 80 90 100
FLRGEKILIL RQTTADWWWG ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE
110 120 130 140 150
EYFGSYGTLK LHLEMLADQP RTTKYHSVIL QNKESLTDKV ILDVGCGTGI
160 170 180 190 200
ISLFCAHYAR PRAVYAVEAS EMAQHTGQLV LQNGFADIIT VYQQKVEDVV
210 220 230 240 250
LPEKVDVLVS EWMGTCLLFE FMIESILYAR DAWLKEDGVI WPTMAALHLV
260 270 280 290 300
PCSADKDYRS KVLFWDNAYE FNLSALKSLA VKEFFSKPKY NHILKPEDCL
310 320 330 340 350
SEPCTILQLD MRTVQISDLE TLRGELRFDI RKAGTLHGFT AWFSVHFQSL
360 370 380 390 400
QEGQPPQVLS TGPFHPTTHW KQTLFMMDDP VPVHTGDVVT GSVVLQRNPV
410 420 430
WRRHMSVALS WAVTSRQDPT SQKVGEKVFP IWR
The sequence of this isoform differs from the canonical sequence as follows:
278-289: SLAVKEFFSKPK → LEKKSSPSGDDS
290-433: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
219-301: FEFMIESILY...HILKPEDCLS → HHTLEADAVH...FGKQCAYLEG
302-433: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
219-423: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
278-433: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8MXR3 | A8MXR3_HUMAN | Protein arginine N-methyltransferas... | PRMT2 | 253 | Annotation score: | ||
H7C2H9 | H7C2H9_HUMAN | Protein arginine N-methyltransferas... | PRMT2 | 194 | Annotation score: | ||
A0A087WYX1 | A0A087WYX1_HUMAN | Protein arginine N-methyltransferas... | PRMT2 | 161 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 256 – 260 | KDYRS → RIIVA in AAB48437 (PubMed:9196002).Curated | 5 | |
Sequence conflicti | 402 | R → I in AAH00727 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042680 | 219 – 433 | FEFMI…FPIWR → AAPLLSCRILPCTCASGPLH VLLACCLPLPCTCASVPLHV LLACCLPVLRAPQPSGLHLS WPIFLL in isoform 2. 1 PublicationAdd BLAST | 215 | |
Alternative sequenceiVSP_054927 | 219 – 423 | Missing in isoform PRMT2Gamma. 1 PublicationAdd BLAST | 205 | |
Alternative sequenceiVSP_043381 | 219 – 320 | Missing in isoform 3. 1 PublicationAdd BLAST | 102 | |
Alternative sequenceiVSP_054928 | 219 – 301 | FEFMI…EDCLS → HHTLEADAVHDGRPSPCPYR RRGHGFSCVAEKPSVEKAHV CGSELGCHFQTRPHISKSWR KSLPHLEMTVDALFGKQCAY LEG in isoform PRMT2Beta. 1 PublicationAdd BLAST | 83 | |
Alternative sequenceiVSP_054929 | 278 – 433 | Missing in isoform PRMT2L2. 1 PublicationAdd BLAST | 156 | |
Alternative sequenceiVSP_054930 | 278 – 289 | SLAVK…FSKPK → LEKKSSPSGDDS in isoform PRMT2Alpha. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_054931 | 290 – 433 | Missing in isoform PRMT2Alpha. 1 PublicationAdd BLAST | 144 | |
Alternative sequenceiVSP_054932 | 302 – 433 | Missing in isoform PRMT2Beta. 1 PublicationAdd BLAST | 132 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000291705; ENSP00000291705; ENSG00000160310 [P55345-6] ENST00000334494; ENSP00000335490; ENSG00000160310 [P55345-2] ENST00000355680; ENSP00000347906; ENSG00000160310 ENST00000397637; ENSP00000380759; ENSG00000160310 ENST00000397638; ENSP00000380760; ENSG00000160310 ENST00000440086; ENSP00000397266; ENSG00000160310 [P55345-3] ENST00000451211; ENSP00000411984; ENSG00000160310 [P55345-4] ENST00000458387; ENSP00000407463; ENSG00000160310 [P55345-5] |
GeneIDi | 3275 |
KEGGi | hsa:3275 |
MANE-Selecti | ENST00000355680.8; ENSP00000347906.3; NM_206962.4; NP_996845.1 |
UCSCi | uc002zjx.5, human [P55345-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X2P | NMR | - | A | 33-87 | [»] | |
SMRi | P55345 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 109511, 69 interactors |
CORUMi | P55345 |
IntActi | P55345, 54 interactors |
MINTi | P55345 |
STRINGi | 9606.ENSP00000380759 |
PTM databases
iPTMneti | P55345 |
PhosphoSitePlusi | P55345 |
SwissPalmi | P55345 |
Genetic variation databases
BioMutai | PRMT2 |
DMDMi | 2499805 |
Proteomic databases
EPDi | P55345 |
jPOSTi | P55345 |
MassIVEi | P55345 |
PaxDbi | P55345 |
PeptideAtlasi | P55345 |
PRIDEi | P55345 |
ProteomicsDBi | 56853 [P55345-1] 56854 [P55345-2] 56855 [P55345-3] 6261 6262 6263 |
Protocols and materials databases
Antibodypediai | 10796, 454 antibodies from 31 providers |
DNASUi | 3275 |
Genome annotation databases
Organism-specific databases
CTDi | 3275 |
DisGeNETi | 3275 |
GeneCardsi | PRMT2 |
HGNCi | HGNC:5186, PRMT2 |
HPAi | ENSG00000160310, Low tissue specificity |
MIMi | 601961, gene |
neXtProti | NX_P55345 |
OpenTargetsi | ENSG00000160310 |
PharmGKBi | PA29460 |
VEuPathDBi | HostDB:ENSG00000160310 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1499, Eukaryota |
GeneTreei | ENSGT00940000160683 |
HOGENOMi | CLU_017375_4_0_1 |
InParanoidi | P55345 |
OMAi | GTKSFPM |
PhylomeDBi | P55345 |
TreeFami | TF332196 |
Enzyme and pathway databases
BRENDAi | 2.1.1.319, 2681 |
PathwayCommonsi | P55345 |
SignaLinki | P55345 |
Miscellaneous databases
BioGRID-ORCSi | 3275, 16 hits in 1052 CRISPR screens |
ChiTaRSi | PRMT2, human |
EvolutionaryTracei | P55345 |
GeneWikii | PRMT2 |
GenomeRNAii | 3275 |
Pharosi | P55345, Tbio |
PROi | PR:P55345 |
RNActi | P55345, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000160310, Expressed in cortical plate and 241 other tissues |
ExpressionAtlasi | P55345, baseline and differential |
Genevisiblei | P55345, HS |
Family and domain databases
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR029063, SAM-dependent_MTases IPR036028, SH3-like_dom_sf IPR001452, SH3_domain IPR007848, Small_mtfrase_dom |
PANTHERi | PTHR11006, PTHR11006, 1 hit |
Pfami | View protein in Pfam PF05175, MTS, 1 hit PF00018, SH3_1, 1 hit |
PRINTSi | PR00452, SH3DOMAIN |
SMARTi | View protein in SMART SM00326, SH3, 1 hit |
SUPFAMi | SSF50044, SSF50044, 1 hit SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit PS50002, SH3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ANM2_HUMAN | |
Accessioni | P55345Primary (citable) accession number: P55345 Secondary accession number(s): B7U630 Q9UMC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families