UniProtKB - P55263 (ADK_HUMAN)
Protein
Adenosine kinase
Gene
ADK
Organism
Homo sapiens (Human)
Status
Functioni
ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Catalytic activityi
- EC:2.7.1.20
Cofactori
Mg2+Note: Binds 3 Mg2+ ions per subunit.
: AMP biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes AMP from adenosine.Proteins known to be involved in this subpathway in this organism are:
- Adenosine kinase (ADK), Adenosine kinase (ADK), Adenosine kinase (ADK), Adenosine kinase (ADK), Adenosine kinase, Adenosine kinase (ADK), Adenosine kinase (ADK), Adenosine kinase (ADK), Adenosine kinase
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenosine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 49 | Magnesium 1 | 1 | |
Metal bindingi | 147 | Magnesium 2 | 1 | |
Metal bindingi | 148 | Magnesium 2 | 1 | |
Active sitei | 317 | 1 |
GO - Molecular functioni
- adenosine kinase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB
GO - Biological processi
- AMP salvage Source: UniProtKB-UniPathway
- dATP biosynthetic process Source: Ensembl
- purine-containing compound salvage Source: Reactome
- purine ribonucleoside salvage Source: UniProtKB-KW
- ribonucleoside monophosphate biosynthetic process Source: ProtInc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Purine salvage |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08097-MONOMER |
BRENDAi | 2.7.1.20, 2681 |
PathwayCommonsi | P55263 |
Reactomei | R-HSA-74217, Purine salvage |
SABIO-RKi | P55263 |
UniPathwayi | UPA00588;UER00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosine kinase (EC:2.7.1.20)Short name: AK Alternative name(s): Adenosine 5'-phosphotransferase |
Gene namesi | Name:ADK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000156110.13 |
HGNCi | HGNC:257, ADK |
MIMi | 102750, gene |
neXtProti | NX_P55263 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Involvement in diseasei
Hypermethioninemia due to adenosine kinase deficiency (HMAKD)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_066640 | 30 | G → E in HMAKD; the mutant shows some residual activity. 1 PublicationCorresponds to variant dbSNP:rs397514454EnsemblClinVar. | 1 | |
Natural variantiVAR_066641 | 235 | D → A in HMAKD; the mutant shows some residual activity. 1 PublicationCorresponds to variant dbSNP:rs397514453EnsemblClinVar. | 1 | |
Natural variantiVAR_066642 | 318 | A → E in HMAKD; complete loss of activity. 1 PublicationCorresponds to variant dbSNP:rs397514452EnsemblClinVar. | 1 |
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 – 12 | KK → AA or AD: Abolishes nuclear localization. 1 Publication | 2 |
Keywords - Diseasei
Disease mutationOrganism-specific databases
DisGeNETi | 132 |
MalaCardsi | ADK |
MIMi | 614300, phenotype |
OpenTargetsi | ENSG00000156110 |
Orphaneti | 289290, Hypermethioninemia encephalopathy due to adenosine kinase deficiency |
PharmGKBi | PA24579 |
Miscellaneous databases
Pharosi | P55263, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3589 |
DrugBanki | DB07280, 5-[4-(DIMETHYLAMINO)PHENYL]-6-[(6-MORPHOLIN-4-YLPYRIDIN-3-YL)ETHYNYL]PYRIMIDIN-4-AMINE DB07173, 7-(5-DEOXY-BETA-D-RIBOFURANOSYL)-5-IODO-7H-PYRROLO[2,3-D]PYRIMIDIN-4-AMINE DB01048, Abacavir DB00640, Adenosine DB00131, Adenosine phosphate DB00171, ATP DB00811, Ribavirin |
DrugCentrali | P55263 |
GuidetoPHARMACOLOGYi | 1231 |
Polymorphism and mutation databases
BioMutai | ADK |
DMDMi | 6840802 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000080053 | 2 – 362 | Adenosine kinaseAdd BLAST | 361 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 77 | Phosphotyrosine1 Publication | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-162 |
EPDi | P55263 |
jPOSTi | P55263 |
MassIVEi | P55263 |
MaxQBi | P55263 |
PaxDbi | P55263 |
PeptideAtlasi | P55263 |
PRIDEi | P55263 |
ProteomicsDBi | 56823 [P55263-1] 56824 [P55263-2] 56825 [P55263-3] 6910 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00234368 |
PTM databases
iPTMneti | P55263 |
MetOSitei | P55263 |
PhosphoSitePlusi | P55263 |
SwissPalmi | P55263 |
Expressioni
Tissue specificityi
Widely expressed. Highest level in placenta, liver, muscle and kidney.
Gene expression databases
Bgeei | ENSG00000156110, Expressed in cartilage tissue and 235 other tissues |
ExpressionAtlasi | P55263, baseline and differential |
Genevisiblei | P55263, HS |
Organism-specific databases
HPAi | ENSG00000156110, Tissue enhanced (liver) |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 106644, 19 interactors |
IntActi | P55263, 5 interactors |
STRINGi | 9606.ENSP00000286621 |
Chemistry databases
BindingDBi | P55263 |
Miscellaneous databases
RNActi | P55263, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P55263 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55263 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 8 – 16 | Nuclear localization signal1 Publication | 9 |
Sequence similaritiesi
Belongs to the carbohydrate kinase PfkB family.Curated
Phylogenomic databases
eggNOGi | KOG2854, Eukaryota |
GeneTreei | ENSGT00390000014320 |
HOGENOMi | CLU_045832_0_0_1 |
InParanoidi | P55263 |
OMAi | APFIAQF |
OrthoDBi | 1226324at2759 |
PhylomeDBi | P55263 |
TreeFami | TF300745 |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR001805, Adenokinase IPR002173, Carboh/pur_kinase_PfkB_CS IPR011611, PfkB_dom IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00989, ADENOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P55263-1) [UniParc]FASTAAdd to basket
Also known as: Long
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAAEEEPKP KKLKVEAPQA LRENILFGMG NPLLDISAVV DKDFLDKYSL
60 70 80 90 100
KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA
110 120 130 140 150
ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS
160 170 180 190 200
LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA
210 220 230 240 250
HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE
260 270 280 290 300
QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA
310 320 330 340 350
VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR
360
TGCTFPEKPD FH
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5F9ZHJ1 | A0A5F9ZHJ1_HUMAN | Adenosine kinase | ADK | 270 | Annotation score: | ||
A0A5F9ZH72 | A0A5F9ZH72_HUMAN | Adenosine kinase | ADK | 229 | Annotation score: | ||
A0A5F9ZH31 | A0A5F9ZH31_HUMAN | Adenosine kinase | ADK | 294 | Annotation score: | ||
A0A5K1VW54 | A0A5K1VW54_HUMAN | Adenosine kinase | ADK | 347 | Annotation score: | ||
A0A5F9ZGZ8 | A0A5F9ZGZ8_HUMAN | Adenosine kinase | ADK | 179 | Annotation score: | ||
A0A5K1VW94 | A0A5K1VW94_HUMAN | Adenosine kinase | ADK | 356 | Annotation score: | ||
A0A5F9ZH83 | A0A5F9ZH83_HUMAN | Adenosine kinase | ADK | 99 | Annotation score: | ||
A0A5F9ZH76 | A0A5F9ZH76_HUMAN | Adenosine kinase | ADK | 37 | Annotation score: |
Sequence cautioni
The sequence AAB01689 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 98 | H → A AA sequence (PubMed:8577746).Curated | 1 | |
Sequence conflicti | 133 | N → D in AAB01689 (PubMed:8917457).Curated | 1 | |
Sequence conflicti | 171 | K → R in AAB01689 (PubMed:8917457).Curated | 1 | |
Sequence conflicti | 190 | T → H in AAA97893 (PubMed:8577746).Curated | 1 | |
Sequence conflicti | 219 | I → F in AAH03568 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 273 | S → V AA sequence (PubMed:8577746).Curated | 1 | |
Sequence conflicti | 289 | I → N AA sequence (PubMed:8577746).Curated | 1 | |
Sequence conflicti | 307 | K → R in AAB01689 (PubMed:8917457).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_066640 | 30 | G → E in HMAKD; the mutant shows some residual activity. 1 PublicationCorresponds to variant dbSNP:rs397514454EnsemblClinVar. | 1 | |
Natural variantiVAR_066641 | 235 | D → A in HMAKD; the mutant shows some residual activity. 1 PublicationCorresponds to variant dbSNP:rs397514453EnsemblClinVar. | 1 | |
Natural variantiVAR_066642 | 318 | A → E in HMAKD; complete loss of activity. 1 PublicationCorresponds to variant dbSNP:rs397514452EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004668 | 1 – 65 | MAAAE…KHKEL → MTSVRENILFGMGNPLLDIS AVVDKDFLDK in isoform 4. 1 PublicationAdd BLAST | 65 | |
Alternative sequenceiVSP_046713 | 1 – 21 | MAAAE…APQAL → MTSV in isoform 2. 4 PublicationsAdd BLAST | 21 | |
Alternative sequenceiVSP_043526 | 186 – 242 | Missing in isoform 3. 1 PublicationAdd BLAST | 57 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50196 mRNA Translation: AAA97893.1 U33936 mRNA Translation: AAB01689.1 Frameshift. U90338 mRNA Translation: AAB50234.1 U90339 mRNA Translation: AAB50235.1 AK290633 mRNA Translation: BAF83322.1 AK301590 mRNA Translation: BAH13519.1 AK302706 mRNA Translation: BAH13786.1 AC012046 Genomic DNA No translation available. AC022026 Genomic DNA No translation available. AC022540 Genomic DNA No translation available. AC091699 Genomic DNA No translation available. AL357037 Genomic DNA No translation available. AL731576 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54555.1 CH471083 Genomic DNA Translation: EAW54556.1 BC003568 mRNA Translation: AAH03568.1 |
CCDSi | CCDS55716.1 [P55263-3] CCDS55717.1 [P55263-4] CCDS7343.1 [P55263-1] CCDS7344.1 [P55263-2] |
PIRi | JC5363 JC5364 |
RefSeqi | NP_001114.2, NM_001123.3 [P55263-2] NP_001189378.1, NM_001202449.1 [P55263-4] NP_001189379.1, NM_001202450.1 [P55263-3] NP_006712.2, NM_006721.3 [P55263-1] |
Genome annotation databases
Ensembli | ENST00000372734; ENSP00000361819; ENSG00000156110 [P55263-2] ENST00000539909; ENSP00000443965; ENSG00000156110 [P55263-1] ENST00000672429; ENSP00000500292; ENSG00000156110 [P55263-3] ENST00000673027; ENSP00000500201; ENSG00000156110 [P55263-4] |
GeneIDi | 132 |
KEGGi | hsa:132 |
UCSCi | uc001jwi.4, human [P55263-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50196 mRNA Translation: AAA97893.1 U33936 mRNA Translation: AAB01689.1 Frameshift. U90338 mRNA Translation: AAB50234.1 U90339 mRNA Translation: AAB50235.1 AK290633 mRNA Translation: BAF83322.1 AK301590 mRNA Translation: BAH13519.1 AK302706 mRNA Translation: BAH13786.1 AC012046 Genomic DNA No translation available. AC022026 Genomic DNA No translation available. AC022540 Genomic DNA No translation available. AC091699 Genomic DNA No translation available. AL357037 Genomic DNA No translation available. AL731576 Genomic DNA No translation available. CH471083 Genomic DNA Translation: EAW54555.1 CH471083 Genomic DNA Translation: EAW54556.1 BC003568 mRNA Translation: AAH03568.1 |
CCDSi | CCDS55716.1 [P55263-3] CCDS55717.1 [P55263-4] CCDS7343.1 [P55263-1] CCDS7344.1 [P55263-2] |
PIRi | JC5363 JC5364 |
RefSeqi | NP_001114.2, NM_001123.3 [P55263-2] NP_001189378.1, NM_001202449.1 [P55263-4] NP_001189379.1, NM_001202450.1 [P55263-3] NP_006712.2, NM_006721.3 [P55263-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BX4 | X-ray | 1.50 | A | 22-362 | [»] | |
2I6A | X-ray | 2.20 | A/B/C/D | 22-362 | [»] | |
2I6B | X-ray | 2.30 | A/B | 22-362 | [»] | |
4O1L | X-ray | 2.50 | A/B | 17-362 | [»] | |
SMRi | P55263 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106644, 19 interactors |
IntActi | P55263, 5 interactors |
STRINGi | 9606.ENSP00000286621 |
Chemistry databases
BindingDBi | P55263 |
ChEMBLi | CHEMBL3589 |
DrugBanki | DB07280, 5-[4-(DIMETHYLAMINO)PHENYL]-6-[(6-MORPHOLIN-4-YLPYRIDIN-3-YL)ETHYNYL]PYRIMIDIN-4-AMINE DB07173, 7-(5-DEOXY-BETA-D-RIBOFURANOSYL)-5-IODO-7H-PYRROLO[2,3-D]PYRIMIDIN-4-AMINE DB01048, Abacavir DB00640, Adenosine DB00131, Adenosine phosphate DB00171, ATP DB00811, Ribavirin |
DrugCentrali | P55263 |
GuidetoPHARMACOLOGYi | 1231 |
PTM databases
iPTMneti | P55263 |
MetOSitei | P55263 |
PhosphoSitePlusi | P55263 |
SwissPalmi | P55263 |
Polymorphism and mutation databases
BioMutai | ADK |
DMDMi | 6840802 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00234368 |
Proteomic databases
CPTACi | CPTAC-162 |
EPDi | P55263 |
jPOSTi | P55263 |
MassIVEi | P55263 |
MaxQBi | P55263 |
PaxDbi | P55263 |
PeptideAtlasi | P55263 |
PRIDEi | P55263 |
ProteomicsDBi | 56823 [P55263-1] 56824 [P55263-2] 56825 [P55263-3] 6910 |
Protocols and materials databases
Antibodypediai | 29612, 428 antibodies |
DNASUi | 132 |
Genome annotation databases
Ensembli | ENST00000372734; ENSP00000361819; ENSG00000156110 [P55263-2] ENST00000539909; ENSP00000443965; ENSG00000156110 [P55263-1] ENST00000672429; ENSP00000500292; ENSG00000156110 [P55263-3] ENST00000673027; ENSP00000500201; ENSG00000156110 [P55263-4] |
GeneIDi | 132 |
KEGGi | hsa:132 |
UCSCi | uc001jwi.4, human [P55263-1] |
Organism-specific databases
CTDi | 132 |
DisGeNETi | 132 |
EuPathDBi | HostDB:ENSG00000156110.13 |
GeneCardsi | ADK |
HGNCi | HGNC:257, ADK |
HPAi | ENSG00000156110, Tissue enhanced (liver) |
MalaCardsi | ADK |
MIMi | 102750, gene 614300, phenotype |
neXtProti | NX_P55263 |
OpenTargetsi | ENSG00000156110 |
Orphaneti | 289290, Hypermethioninemia encephalopathy due to adenosine kinase deficiency |
PharmGKBi | PA24579 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2854, Eukaryota |
GeneTreei | ENSGT00390000014320 |
HOGENOMi | CLU_045832_0_0_1 |
InParanoidi | P55263 |
OMAi | APFIAQF |
OrthoDBi | 1226324at2759 |
PhylomeDBi | P55263 |
TreeFami | TF300745 |
Enzyme and pathway databases
UniPathwayi | UPA00588;UER00659 |
BioCyci | MetaCyc:HS08097-MONOMER |
BRENDAi | 2.7.1.20, 2681 |
PathwayCommonsi | P55263 |
Reactomei | R-HSA-74217, Purine salvage |
SABIO-RKi | P55263 |
Miscellaneous databases
BioGRID-ORCSi | 132, 3 hits in 848 CRISPR screens |
ChiTaRSi | ADK, human |
EvolutionaryTracei | P55263 |
GeneWikii | ADK_(gene) |
GenomeRNAii | 132 |
Pharosi | P55263, Tchem |
PROi | PR:P55263 |
RNActi | P55263, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000156110, Expressed in cartilage tissue and 235 other tissues |
ExpressionAtlasi | P55263, baseline and differential |
Genevisiblei | P55263, HS |
Family and domain databases
Gene3Di | 3.40.1190.20, 1 hit |
InterProi | View protein in InterPro IPR001805, Adenokinase IPR002173, Carboh/pur_kinase_PfkB_CS IPR011611, PfkB_dom IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00989, ADENOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADK_HUMAN | |
Accessioni | P55263Primary (citable) accession number: P55263 Secondary accession number(s): B7Z783 Q9BTN2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 30, 2000 | |
Last modified: | December 2, 2020 | |
This is version 206 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations