Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis (By similarity). Together with MICALL2, may also regulate adherens junction assembly (PubMed:18094055). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (By similarity). Involved in autophagy (By similarity).By similarity1 Publication

Activity regulationi

Activated in response to insulin.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi17 – 22GTPBy similarity6
Nucleotide bindingi34 – 40GTPBy similarity7
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi152 – 154GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Cilium biogenesis/degradation, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5620916 VxPx cargo-targeting to cilium
R-MMU-8854214 TBC/RABGAPs
R-MMU-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Alternative name(s):
Oncogene c-mel
Gene namesi
Name:Rab8a
Synonyms:Mel, Rab8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96960 Rab8a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211321 – 204Ras-related protein Rab-8AAdd BLAST204
PropeptideiPRO_0000370795205 – 207Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineBy similarity1
Modified residuei185PhosphoserineCombined sources1
Modified residuei204Cysteine methyl esterSequence analysis1
Lipidationi204S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP55258
PaxDbiP55258
PeptideAtlasiP55258
PRIDEiP55258

PTM databases

iPTMnetiP55258
PhosphoSitePlusiP55258
SwissPalmiP55258

Expressioni

Gene expression databases

BgeeiENSMUSG00000003037 Expressed in 283 organ(s), highest expression level in small intestine
CleanExiMM_RAB8A
ExpressionAtlasiP55258 baseline and differential
GenevisibleiP55258 MM

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3 and EHBP1L1; two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts (GTP-bound form) with EHBP1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1 (PubMed:19625297). Interacts with DCDC5 (By similarity).By similarity8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201387, 9 interactors
CORUMiP55258
DIPiDIP-31053N
IntActiP55258, 24 interactors
MINTiP55258
STRINGi10090.ENSMUSP00000003121

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP55258
SMRiP55258
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55258

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP55258
KOiK07901
OMAiNPQGSNQ
OrthoDBiEOG091G0LA6
TreeFamiTF314097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P55258-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS

FFRCSLL
Length:207
Mass (Da):23,668
Last modified:November 22, 2005 - v2
Checksum:iAC89DC85588FB8F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176 – 183KLEGNSPQ → NWKATAA in AAB19682 (PubMed:1886711).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53270 mRNA Translation: AAB19682.1
AK076048 mRNA Translation: BAC36146.1
AK079306 mRNA Translation: BAC37603.1
AK080740 mRNA Translation: BAC38003.1
BC019990 mRNA Translation: AAH19990.1
CCDSiCCDS22409.1
RefSeqiNP_075615.2, NM_023126.2
UniGeneiMm.162811

Genome annotation databases

EnsembliENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037
GeneIDi17274
KEGGimmu:17274
UCSCiuc009mfj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S53270 mRNA Translation: AAB19682.1
AK076048 mRNA Translation: BAC36146.1
AK079306 mRNA Translation: BAC37603.1
AK080740 mRNA Translation: BAC38003.1
BC019990 mRNA Translation: AAH19990.1
CCDSiCCDS22409.1
RefSeqiNP_075615.2, NM_023126.2
UniGeneiMm.162811

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FU5X-ray2.00C/D1-175[»]
ProteinModelPortaliP55258
SMRiP55258
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201387, 9 interactors
CORUMiP55258
DIPiDIP-31053N
IntActiP55258, 24 interactors
MINTiP55258
STRINGi10090.ENSMUSP00000003121

PTM databases

iPTMnetiP55258
PhosphoSitePlusiP55258
SwissPalmiP55258

Proteomic databases

EPDiP55258
PaxDbiP55258
PeptideAtlasiP55258
PRIDEiP55258

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003121; ENSMUSP00000003121; ENSMUSG00000003037
GeneIDi17274
KEGGimmu:17274
UCSCiuc009mfj.1 mouse

Organism-specific databases

CTDi4218
MGIiMGI:96960 Rab8a

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP55258
KOiK07901
OMAiNPQGSNQ
OrthoDBiEOG091G0LA6
TreeFamiTF314097

Enzyme and pathway databases

ReactomeiR-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5620916 VxPx cargo-targeting to cilium
R-MMU-8854214 TBC/RABGAPs
R-MMU-8873719 RAB geranylgeranylation

Miscellaneous databases

EvolutionaryTraceiP55258
PROiPR:P55258
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003037 Expressed in 283 organ(s), highest expression level in small intestine
CleanExiMM_RAB8A
ExpressionAtlasiP55258 baseline and differential
GenevisibleiP55258 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAB8A_MOUSE
AccessioniPrimary (citable) accession number: P55258
Secondary accession number(s): Q8VCF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 22, 2005
Last modified: October 10, 2018
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again