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Protein

RTX-I toxin determinant A from serotypes 1/9

Gene

apxIA

Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

One of the virulence factors of A.pleuropneumoniae, which has a strong hemolytic activity and is cytotoxic for alveolar macrophages and neutrophils.

Miscellaneous

ApxIA is partially deleted in serotypes 2, 4, 6, 7, 8, 12, and totally deleted in serotype 3.
The sequence shown is that of serotype 1.
In strain CM15 (serotype 1), disruption of this gene results in production of only RTX-II toxin, and a weak hemolytic activity in mice and pigs.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionToxin
Biological processCytolysis, Hemolysis, Virulence
LigandCalcium

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
RTX-I toxin determinant A from serotypes 1/9
Alternative name(s):
APX-IA
ApxI
Cytolysin IA
Short name:
CLY-IA
Hemolysin IA
Short name:
HLY-IA
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:apxIA
Synonyms:clyIA, hlyIA
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiActinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri715 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei226 – 256HelicalSequence analysisAdd BLAST31
Transmembranei297 – 326HelicalSequence analysisAdd BLAST30
Transmembranei367 – 406HelicalSequence analysisAdd BLAST40

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Secreted

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001962371 – 1023RTX-I toxin determinant A from serotypes 1/9Add BLAST1023

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Palmitoylated by ApxIC. The toxin only becomes active when modified (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Palmitate

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
P55128

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P55128

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati730 – 747Hemolysin-type calcium-binding 1Add BLAST18
Repeati748 – 765Hemolysin-type calcium-binding 2Add BLAST18
Repeati766 – 783Hemolysin-type calcium-binding 3Add BLAST18
Repeati784 – 801Hemolysin-type calcium-binding 4Add BLAST18
Repeati812 – 829Hemolysin-type calcium-binding 5Add BLAST18
Repeati830 – 847Hemolysin-type calcium-binding 6Add BLAST18

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The Gly-rich region is probably involved in binding calcium, which is required for target cell-binding or cytolytic activity.
The three transmembrane domains are believed to be involved in pore formation by the cytotoxin.By similarity

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4107V48 Bacteria
ENOG410Z1F3 LUCA

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
2.150.10.10, 2 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR018511 Hemolysin-typ_Ca-bd_CS
IPR001343 Hemolysn_Ca-bd
IPR013550 RTX_C
IPR018504 RTX_N
IPR003995 RTX_toxin_determinant-A
IPR011049 Serralysin-like_metalloprot_C

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00353 HemolysinCabind, 4 hits
PF02382 RTX, 1 hit
PF08339 RTX_C, 1 hit

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR01488 RTXTOXINA

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51120 SSF51120, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00330 HEMOLYSIN_CALCIUM, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P55128-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANSQLDRVK GLIDSLNQHT KSAAKSGAGA LKNGLGQVKQ AGQKLILYIP
60 70 80 90 100
KDYQASTGSS LNDLVKAAEA LGIEVHRSEK NGTALAKELF GTTEKLLGFS
110 120 130 140 150
ERGIALFAPQ FDKLLNKNQK LSKSLGGSSE ALGQRLNKTQ TALSALQSFL
160 170 180 190 200
GTAIAGMDLD SLLRRRRNGE DVSGSELAKA GVDLAAQLVD NIASATGTVD
210 220 230 240 250
AFAEQLGKLA MPYLTLALSG LASKLNNLPD LSLAGPGFDA VSGILSVVSA
260 270 280 290 300
SFILSNKDAD AGTKAAAGIE ISTKILGNIG KAVSQYIIAQ RVAAGLSTTA
310 320 330 340 350
ATGGLIGSVV ALAISPLSFL NVADKFERAK QLEQYSERFK KFGYEGDSLL
360 370 380 390 400
ASFYRETGAI EAALTTINSV LSARSAGVGA AATGSLVGAP VAALVSAITG
410 420 430 440 450
IISGILDASK QAIFERVATK LANKIDEWEK KHGKNYFENG YDARHSAFLE
460 470 480 490 500
DTFELLSQYN KEYSVERVVA ITQQRWDVNI GELAGITRKG SDTKSGKAYV
510 520 530 540 550
DFFEEGKLLE KEPDRFDKKV FDPLEGKIDL SSINKTTLLK FVTPVFTAGE
560 570 580 590 600
EIRERKQTGK YQYMTELFVK GKEKWVVTGV QSHNAIYDYT NLIQLAIDKK
610 620 630 640 650
GEKRQVTIES HLGEKNDRIY LSSGSSIVYA GNGHDVAYYD KTDTGYLTFD
660 670 680 690 700
GQSAQKAGEY IVTKELKADV KVLKEVVKTQ DISVGKTCSE KLEYRDYELS
710 720 730 740 750
PFELGNGIRA KDELHSVEEI IGSNRKDKFF GSRFTDIFHG AKGDDEIYGN
760 770 780 790 800
DGHDILYGDD GNDVIHGGDG NDHLVGGNGN DRLIGGKGNN FLNGGDGDDE
810 820 830 840 850
LQVFEGQYNV LLGGAGNDIL YGSDGTNLFD GGVGNDKIYG GLGKDIYRYS
860 870 880 890 900
KEYGRHIIIE KGGDDDTLLL SDLSFKDVGF IRIGDDLLVN KRIGGTLYYH
910 920 930 940 950
EDYNGNALTI KDWFKEGKEG QNNKIEKIVD KDGAYVLSQY LTELTAPGRG
960 970 980 990 1000
INYFNGLEEK LYYGEGYNAL PQLRKDIEQI ISSTGAFTGD HGKVSVGSGG
1010 1020
PLVYNNSANN VANSLSYSLA QAA
Length:1,023
Mass (Da):110,194
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF99A88CFC9F1A598
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti210 – 217AMPYLTLA → GNALSNTR (PubMed:8359891).Curated8
Sequence conflicti210 – 217AMPYLTLA → GNALSNTR (Ref. 4) Curated8
Sequence conflicti374R → A (PubMed:8359891).Curated1
Sequence conflicti374R → A (Ref. 4) Curated1
Sequence conflicti562Q → E (PubMed:8359891).Curated1
Sequence conflicti562Q → E (Ref. 4) Curated1
Sequence conflicti687 – 688TC → R (PubMed:8359891).Curated2
Sequence conflicti687 – 688TC → R (Ref. 4) Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X52899 Genomic DNA Translation: CAA37081.1
X68595 Genomic DNA Translation: CAA48586.1
X73117 Genomic DNA Translation: CAA51548.1
U05042 Genomic DNA Translation: AAB05034.1

Protein sequence database of the Protein Information Resource

More...
PIRi
I39643

NCBI Reference Sequences

More...
RefSeqi
WP_005598583.1, NZ_CP030753.1

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52899 Genomic DNA Translation: CAA37081.1
X68595 Genomic DNA Translation: CAA48586.1
X73117 Genomic DNA Translation: CAA51548.1
U05042 Genomic DNA Translation: AAB05034.1
PIRiI39643
RefSeqiWP_005598583.1, NZ_CP030753.1

3D structure databases

ProteinModelPortaliP55128
SMRiP55128
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4107V48 Bacteria
ENOG410Z1F3 LUCA

Family and domain databases

Gene3Di2.150.10.10, 2 hits
InterProiView protein in InterPro
IPR018511 Hemolysin-typ_Ca-bd_CS
IPR001343 Hemolysn_Ca-bd
IPR013550 RTX_C
IPR018504 RTX_N
IPR003995 RTX_toxin_determinant-A
IPR011049 Serralysin-like_metalloprot_C
PfamiView protein in Pfam
PF00353 HemolysinCabind, 4 hits
PF02382 RTX, 1 hit
PF08339 RTX_C, 1 hit
PRINTSiPR01488 RTXTOXINA
SUPFAMiSSF51120 SSF51120, 1 hit
PROSITEiView protein in PROSITE
PS00330 HEMOLYSIN_CALCIUM, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRTX11_ACTPL
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P55128
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: December 5, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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