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UniProtKB - P55097 (CATK_MOUSE)
Protein
Cathepsin K
Gene
Ctsk
Organism
Mus musculus (Mouse)
Status
Functioni
Thiol protease involved in osteoclastic bone resorption. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation (By similarity).
Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676).
By similarity1 PublicationCatalytic activityi
- Broad proteolytic activity. With small-molecule substrates and inhibitors, the major determinant of specificity is P2, which is preferably Leu, Met > Phe, and not Arg. EC:3.4.22.38
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 139 | By similarity | 1 | |
Active sitei | 276 | By similarity | 1 | |
Active sitei | 296 | By similarity | 1 |
GO - Molecular functioni
- collagen binding Source: MGI
- cysteine-type endopeptidase activity Source: MGI
- cysteine-type peptidase activity Source: MGI
- fibronectin binding Source: MGI
- proteoglycan binding Source: MGI
GO - Biological processi
- bone resorption Source: MGI
- collagen catabolic process Source: MGI
- immune response Source: GO_Central
- intramembranous ossification Source: Ensembl
- negative regulation of cartilage development Source: MGI
- proteolysis involved in cellular protein catabolic process Source: UniProtKB
- thyroid hormone generation Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.38, 3474 |
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-1679131, Trafficking and processing of endosomal TLR R-MMU-2132295, MHC class II antigen presentation R-MMU-8939242, RUNX1 regulates transcription of genes involved in differentiation of keratinocytes |
Protein family/group databases
MEROPSi | I29.007 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ctsk |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107823, Ctsk |
VEuPathDBi | HostDB:ENSMUSG00000028111 |
Subcellular locationi
Lysosome
- Lysosome 1 Publication
Plasma membrane
- Apical cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Extracellular side 1 Publication
Extracellular region or secreted
- Secreted 1 Publication
Note: Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells.1 Publication
Extracellular region or secreted
- extracellular space Source: UniProtKB
Lysosome
- lysosome Source: UniProtKB
Nucleus
- nucleoplasm Source: MGI
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
- external side of plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
Cell membrane, Lysosome, Membrane, SecretedPathology & Biotechi
Disruption phenotypei
Enlarged thyroid follicles, reduced extension of the thyroid epithelium, and slight increase in the levels of Tg/thyroglobulin in the thyroid follicles. Loss of localization of CTSB/cathepsin B and L to the apical membrane of thyroid epithelial cells. Serum levels of thyroid hormone thyroxine (T4) are normal. However, reduction in T4 levels is more severe in CTSK and CTSL double knockout mice compared to CTSL double knockout mice.1 Publication
Chemistry databases
ChEMBLi | CHEMBL1075277 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Sequence analysisAdd BLAST | 15 | |
PropeptideiPRO_0000026301 | 16 – 114 | Activation peptideAdd BLAST | 99 | |
ChainiPRO_0000026302 | 115 – 329 | Cathepsin KAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 136 ↔ 177 | By similarity | ||
Disulfide bondi | 170 ↔ 210 | By similarity | ||
Glycosylationi | 213 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 269 ↔ 318 | By similarity |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P55097 |
PeptideAtlasi | P55097 |
PRIDEi | P55097 |
ProteomicsDBi | 279921 |
PTM databases
GlyGeni | P55097, 2 sites |
iPTMneti | P55097 |
PhosphoSitePlusi | P55097 |
Expressioni
Tissue specificityi
Predominantly expressed in bones (PubMed:8814310). Expressed in thyroid epithelial cells (PubMed:12782676).2 Publications
Developmental stagei
Expressed in new born and adults.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000028111, Expressed in hindlimb long bone and 232 other tissues |
Genevisiblei | P55097, MM |
Interactioni
GO - Molecular functioni
- fibronectin binding Source: MGI
- proteoglycan binding Source: MGI
Protein-protein interaction databases
IntActi | P55097, 1 interactor |
MINTi | P55097 |
STRINGi | 10090.ENSMUSP00000015664 |
Miscellaneous databases
RNActi | P55097, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P55097 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000157759 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | P55097 |
OMAi | CNKEDIN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | P55097 |
TreeFami | TF313739 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR015644, Peptidase_C1A_cathepsin-K IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
PANTHERi | PTHR12411:SF55, PTHR12411:SF55, 1 hit |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P55097-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MWVFKFLLLP MVSFALSPEE MLDTQWELWK KTHQKQYNSK VDEISRRLIW
60 70 80 90 100
EKNLKQISAH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLRIPPSRS
110 120 130 140 150
YSNDTLYTPE WEGRVPDSID YRKKGYVTPV KNQGQCGSCW AFSSAGALEG
160 170 180 190 200
QLKKKTGKLL ALSPQNLVDC VTENYGCGGG YMTTAFQYVQ QNGGIDSEDA
210 220 230 240 250
YPYVGQDESC MYNATAKAAK CRGYREIPVG NEKALKRAVA RVGPISVSID
260 270 280 290 300
ASLASFQFYS RGVYYDENCD RDNVNHAVLV VGYGTQKGSK HWIIKNSWGE
310 320
SWGNKGYALL ARNKNNACGI TNMASFPKM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 – 6 | VFKF → GLKV in CAA64218 (PubMed:8814310).Curated | 4 | |
Sequence conflicti | 201 | Y → F in CAA64218 (PubMed:8814310).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94444 mRNA Translation: CAA64218.1 AJ006033 Genomic DNA Translation: CAA06825.1 BC046320 mRNA Translation: AAH46320.1 |
CCDSi | CCDS17615.1 |
PIRi | S74227 |
RefSeqi | NP_031828.2, NM_007802.4 XP_006501037.1, XM_006500974.3 |
Genome annotation databases
Ensembli | ENSMUST00000015664; ENSMUSP00000015664; ENSMUSG00000028111 |
GeneIDi | 13038 |
KEGGi | mmu:13038 |
UCSCi | uc008qjy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X94444 mRNA Translation: CAA64218.1 AJ006033 Genomic DNA Translation: CAA06825.1 BC046320 mRNA Translation: AAH46320.1 |
CCDSi | CCDS17615.1 |
PIRi | S74227 |
RefSeqi | NP_031828.2, NM_007802.4 XP_006501037.1, XM_006500974.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5T6U | X-ray | 2.90 | A | 115-329 | [»] | |
6BKI | X-ray | 2.94 | A/B | 115-329 | [»] | |
SMRi | P55097 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P55097, 1 interactor |
MINTi | P55097 |
STRINGi | 10090.ENSMUSP00000015664 |
Chemistry databases
ChEMBLi | CHEMBL1075277 |
Protein family/group databases
MEROPSi | I29.007 |
PTM databases
GlyGeni | P55097, 2 sites |
iPTMneti | P55097 |
PhosphoSitePlusi | P55097 |
Proteomic databases
PaxDbi | P55097 |
PeptideAtlasi | P55097 |
PRIDEi | P55097 |
ProteomicsDBi | 279921 |
Protocols and materials databases
Antibodypediai | 34039, 604 antibodies from 37 providers |
DNASUi | 13038 |
Genome annotation databases
Ensembli | ENSMUST00000015664; ENSMUSP00000015664; ENSMUSG00000028111 |
GeneIDi | 13038 |
KEGGi | mmu:13038 |
UCSCi | uc008qjy.2, mouse |
Organism-specific databases
CTDi | 1513 |
MGIi | MGI:107823, Ctsk |
VEuPathDBi | HostDB:ENSMUSG00000028111 |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000157759 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | P55097 |
OMAi | CNKEDIN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | P55097 |
TreeFami | TF313739 |
Enzyme and pathway databases
BRENDAi | 3.4.22.38, 3474 |
Reactomei | R-MMU-1442490, Collagen degradation R-MMU-1474228, Degradation of the extracellular matrix R-MMU-1592389, Activation of Matrix Metalloproteinases R-MMU-1679131, Trafficking and processing of endosomal TLR R-MMU-2132295, MHC class II antigen presentation R-MMU-8939242, RUNX1 regulates transcription of genes involved in differentiation of keratinocytes |
Miscellaneous databases
BioGRID-ORCSi | 13038, 1 hit in 64 CRISPR screens |
ChiTaRSi | Ctsk, mouse |
PROi | PR:P55097 |
RNActi | P55097, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028111, Expressed in hindlimb long bone and 232 other tissues |
Genevisiblei | P55097, MM |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR015644, Peptidase_C1A_cathepsin-K IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
PANTHERi | PTHR12411:SF55, PTHR12411:SF55, 1 hit |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CATK_MOUSE | |
Accessioni | P55097Primary (citable) accession number: P55097 Secondary accession number(s): O88718 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 169 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families