UniProtKB - P55087 (AQP4_HUMAN)
Aquaporin-4
AQP4
Functioni
GO - Molecular functioni
- identical protein binding Source: IntAct
- water channel activity Source: UniProtKB
GO - Biological processi
- cellular response to interferon-gamma Source: UniProtKB
- cellular water homeostasis Source: UniProtKB
- cerebrospinal fluid circulation Source: UniProtKB
- multicellular organismal water homeostasis Source: UniProtKB
- protein homotetramerization Source: UniProtKB
- renal water homeostasis Source: Reactome
- water homeostasis Source: UniProtKB
- water transport Source: UniProtKB
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | P55087 |
Reactomei | R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-432047, Passive transport by Aquaporins |
SIGNORi | P55087 |
Protein family/group databases
TCDBi | 1.A.8.8.5, the major intrinsic protein (mip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Aquaporin-4Short name: AQP-4 Alternative name(s): Mercurial-insensitive water channel1 Publication Short name: MIWC1 Publication WCH4 |
Gene namesi | Name:AQP4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000171885.13 |
HGNCi | HGNC:637, AQP4 |
MIMi | 600308, gene |
neXtProti | NX_P55087 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein 1 Publication
- Basolateral cell membrane By similarity; Multi-pass membrane protein 1 Publication
- sarcolemma 1 Publication; Multi-pass membrane protein 1 Publication
Endosome
- Endosome membrane By similarity
Other locations
- Cell projection By similarity
Note: Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries.By similarity
Endosome
- endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: GOC
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- external side of plasma membrane Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
- sarcolemma Source: UniProtKB
Other locations
- astrocyte end-foot Source: UniProtKB
- cytoplasm Source: UniProtKB
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 36 | Cytoplasmic1 PublicationAdd BLAST | 36 | |
Transmembranei | 37 – 57 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 58 – 69 | Extracellular1 PublicationAdd BLAST | 12 | |
Transmembranei | 70 – 89 | Helical1 PublicationAdd BLAST | 20 | |
Topological domaini | 90 – 93 | Cytoplasmic1 Publication | 4 | |
Intramembranei | 94 – 101 | Discontinuously helical1 Publication | 8 | |
Topological domaini | 102 – 115 | Cytoplasmic1 PublicationAdd BLAST | 14 | |
Transmembranei | 116 – 136 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 137 – 155 | Extracellular1 PublicationAdd BLAST | 19 | |
Transmembranei | 156 – 176 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 177 – 184 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 185 – 205 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 206 – 208 | Extracellular1 Publication | 3 | |
Intramembranei | 209 – 222 | Discontinuously helical1 PublicationAdd BLAST | 14 | |
Topological domaini | 223 – 231 | Extracellular1 Publication | 9 | |
Transmembranei | 232 – 252 | Helical1 PublicationAdd BLAST | 21 | |
Topological domaini | 253 – 323 | Cytoplasmic1 PublicationAdd BLAST | 71 |
Keywords - Cellular componenti
Cell membrane, Cell projection, Endosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 361 |
OpenTargetsi | ENSG00000171885 |
PharmGKBi | PA24922 |
Miscellaneous databases
Pharosi | P55087, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5964 |
Polymorphism and mutation databases
BioMutai | AQP4 |
DMDMi | 2506859 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063948 | 1 – 323 | Aquaporin-4Add BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 13 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 17 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 111 | Phosphoserine; by PKGBy similarity | 1 | |
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 180 | Phosphoserine; by PKCBy similarity | 1 | |
Glycosylationi | 206 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 276 | PhosphoserineBy similarity | 1 | |
Modified residuei | 285 | PhosphoserineBy similarity | 1 | |
Modified residuei | 289 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 321 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Lipoprotein, Palmitate, PhosphoproteinProteomic databases
MassIVEi | P55087 |
PaxDbi | P55087 |
PeptideAtlasi | P55087 |
PRIDEi | P55087 |
ProteomicsDBi | 56788 [P55087-1] 56789 [P55087-2] |
PTM databases
GlyGeni | P55087, 2 sites |
iPTMneti | P55087 |
PhosphoSitePlusi | P55087 |
SwissPalmi | P55087 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000171885, Expressed in lateral globus pallidus and 172 other tissues |
ExpressionAtlasi | P55087, baseline and differential |
Genevisiblei | P55087, HS |
Organism-specific databases
HPAi | ENSG00000171885, Group enriched (brain, lung) |
Interactioni
Subunit structurei
Homotetramer (PubMed:19383790). The tetramers can form oligomeric arrays in membranes. The size of the oligomers differs between tissues and is smaller in skeletal muscle than in brain. Interaction between AQP4 oligomeric arrays in close-by cells can contribute to cell-cell adhesion (By similarity).
Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1 (PubMed:22328087).
By similarity2 PublicationsBinary interactionsi
Hide detailsP55087
With | #Exp. | IntAct |
---|---|---|
CREB3 - isoform 1 [O43889-2] | 3 | EBI-10104898,EBI-625022 |
GJA8 [P48165] | 3 | EBI-10104898,EBI-17458373 |
HPCAL4 [Q9UM19] | 3 | EBI-10104898,EBI-744820 |
WLS - isoform 1 [Q5T9L3-1] | 3 | EBI-10104898,EBI-22114623 |
AQP4 - isoform 2 [P55087-1]
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-15771758,EBI-15771758 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 106857, 5 interactors |
CORUMi | P55087 |
DIPi | DIP-48842N |
IntActi | P55087, 5 interactors |
STRINGi | 9606.ENSP00000372654 |
Miscellaneous databases
RNActi | P55087, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P55087 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55087 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 97 – 99 | NPA 11 Publication | 3 | |
Motifi | 213 – 215 | NPA 21 Publication | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000156037 |
InParanoidi | P55087 |
OMAi | NWGGSEK |
OrthoDBi | 1152704at2759 |
PhylomeDBi | P55087 |
TreeFami | TF312940 |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSDRPTARRW GKCGPLCTRE NIMVAFKGVW TQAFWKAVTA EFLAMLIFVL
60 70 80 90 100
LSLGSTINWG GTEKPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV
110 120 130 140 150
TVAMVCTRKI SIAKSVFYIA AQCLGAIIGA GILYLVTPPS VVGGLGVTMV
160 170 180 190 200
HGNLTAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGFSVAIG
210 220 230 240 250
HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY
260 270 280 290 300
VFCPDVEFKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVH
310 320
VIDVDRGEEK KGKDQSGEVL SSV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5F9ZHR4 | A0A5F9ZHR4_HUMAN | Aquaporin-4 | AQP4 | 352 | Annotation score: | ||
V9PBN7 | V9PBN7_HUMAN | Aquaporin 4 isoform delta4 | AQP4 | 296 | Annotation score: | ||
J3KRM4 | J3KRM4_HUMAN | Aquaporin-4 | AQP4 | 185 | Annotation score: | ||
H0Y3H5 | H0Y3H5_HUMAN | Aquaporin-4 | AQP4 | 98 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 246 | G → A in AAC52112 (PubMed:7559426).Curated | 1 | |
Sequence conflicti | 287 – 288 | VE → AK in AAC52112 (PubMed:7559426).Curated | 2 | |
Sequence conflicti | 296 | P → L in AAC52112 (PubMed:7559426).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003232 | 1 – 22 | Missing in isoform 1. CuratedAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34846 mRNA Translation: AAC52112.1 Different initiation. U34845 mRNA Translation: AAC50284.1 D63412 mRNA Translation: BAA09715.1 U63622 mRNA Translation: AAB26957.1 U63623 mRNA Translation: AAB26958.1 AC018371 Genomic DNA No translation available. BC022286 mRNA Translation: AAH22286.1 |
CCDSi | CCDS11889.1 [P55087-1] CCDS58617.1 [P55087-2] |
PIRi | I39178 |
RefSeqi | NP_001641.1, NM_001650.5 [P55087-1] NP_004019.1, NM_004028.4 [P55087-2] |
Genome annotation databases
Ensembli | ENST00000383168; ENSP00000372654; ENSG00000171885 [P55087-1] ENST00000440832; ENSP00000393121; ENSG00000171885 [P55087-2] ENST00000581374; ENSP00000462597; ENSG00000171885 [P55087-2] ENST00000672188; ENSP00000500720; ENSG00000171885 [P55087-1] |
GeneIDi | 361 |
KEGGi | hsa:361 |
UCSCi | uc002kvz.4, human [P55087-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U34846 mRNA Translation: AAC52112.1 Different initiation. U34845 mRNA Translation: AAC50284.1 D63412 mRNA Translation: BAA09715.1 U63622 mRNA Translation: AAB26957.1 U63623 mRNA Translation: AAB26958.1 AC018371 Genomic DNA No translation available. BC022286 mRNA Translation: AAH22286.1 |
CCDSi | CCDS11889.1 [P55087-1] CCDS58617.1 [P55087-2] |
PIRi | I39178 |
RefSeqi | NP_001641.1, NM_001650.5 [P55087-1] NP_004019.1, NM_004028.4 [P55087-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GD8 | X-ray | 1.80 | A | 32-254 | [»] | |
SMRi | P55087 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106857, 5 interactors |
CORUMi | P55087 |
DIPi | DIP-48842N |
IntActi | P55087, 5 interactors |
STRINGi | 9606.ENSP00000372654 |
Chemistry databases
ChEMBLi | CHEMBL5964 |
Protein family/group databases
TCDBi | 1.A.8.8.5, the major intrinsic protein (mip) family |
PTM databases
GlyGeni | P55087, 2 sites |
iPTMneti | P55087 |
PhosphoSitePlusi | P55087 |
SwissPalmi | P55087 |
Polymorphism and mutation databases
BioMutai | AQP4 |
DMDMi | 2506859 |
Proteomic databases
MassIVEi | P55087 |
PaxDbi | P55087 |
PeptideAtlasi | P55087 |
PRIDEi | P55087 |
ProteomicsDBi | 56788 [P55087-1] 56789 [P55087-2] |
Protocols and materials databases
Antibodypediai | 3103, 542 antibodies |
DNASUi | 361 |
Genome annotation databases
Ensembli | ENST00000383168; ENSP00000372654; ENSG00000171885 [P55087-1] ENST00000440832; ENSP00000393121; ENSG00000171885 [P55087-2] ENST00000581374; ENSP00000462597; ENSG00000171885 [P55087-2] ENST00000672188; ENSP00000500720; ENSG00000171885 [P55087-1] |
GeneIDi | 361 |
KEGGi | hsa:361 |
UCSCi | uc002kvz.4, human [P55087-1] |
Organism-specific databases
CTDi | 361 |
DisGeNETi | 361 |
EuPathDBi | HostDB:ENSG00000171885.13 |
GeneCardsi | AQP4 |
HGNCi | HGNC:637, AQP4 |
HPAi | ENSG00000171885, Group enriched (brain, lung) |
MIMi | 600308, gene |
neXtProti | NX_P55087 |
OpenTargetsi | ENSG00000171885 |
PharmGKBi | PA24922 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0223, Eukaryota |
GeneTreei | ENSGT00940000156037 |
InParanoidi | P55087 |
OMAi | NWGGSEK |
OrthoDBi | 1152704at2759 |
PhylomeDBi | P55087 |
TreeFami | TF312940 |
Enzyme and pathway databases
PathwayCommonsi | P55087 |
Reactomei | R-HSA-432040, Vasopressin regulates renal water homeostasis via Aquaporins R-HSA-432047, Passive transport by Aquaporins |
SIGNORi | P55087 |
Miscellaneous databases
BioGRID-ORCSi | 361, 2 hits in 838 CRISPR screens |
ChiTaRSi | AQP4, human |
EvolutionaryTracei | P55087 |
GeneWikii | Aquaporin_4 |
GenomeRNAii | 361 |
Pharosi | P55087, Tbio |
PROi | PR:P55087 |
RNActi | P55087, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000171885, Expressed in lateral globus pallidus and 172 other tissues |
ExpressionAtlasi | P55087, baseline and differential |
Genevisiblei | P55087, HS |
Family and domain databases
CDDi | cd00333, MIP, 1 hit |
Gene3Di | 1.20.1080.10, 1 hit |
InterProi | View protein in InterPro IPR023271, Aquaporin-like IPR034294, Aquaporin_transptr IPR000425, MIP IPR022357, MIP_CS |
PANTHERi | PTHR19139, PTHR19139, 1 hit |
Pfami | View protein in Pfam PF00230, MIP, 1 hit |
PRINTSi | PR00783, MINTRINSICP |
SUPFAMi | SSF81338, SSF81338, 1 hit |
TIGRFAMsi | TIGR00861, MIP, 1 hit |
PROSITEi | View protein in PROSITE PS00221, MIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AQP4_HUMAN | |
Accessioni | P55087Primary (citable) accession number: P55087 Secondary accession number(s): P78564 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 18
Human chromosome 18: entries, gene names and cross-references to MIM