Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aquaporin-4

Gene

AQP4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.

GO - Molecular functioni

GO - Biological processi

  • cellular response to interferon-gamma Source: UniProtKB
  • ion transmembrane transport Source: GO_Central
  • multicellular organismal water homeostasis Source: UniProtKB
  • renal water homeostasis Source: Reactome
  • water transport Source: Reactome

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-432047 Passive transport by Aquaporins
SIGNORiP55087

Protein family/group databases

TCDBi1.A.8.8.5 the major intrinsic protein (mip) family

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin-4
Short name:
AQP-4
Alternative name(s):
Mercurial-insensitive water channel
Short name:
MIWC
WCH4
Gene namesi
Name:AQP4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000171885.13
HGNCiHGNC:637 AQP4
MIMi600308 gene
neXtProtiNX_P55087

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 36CytoplasmicSequence analysisAdd BLAST36
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 64ExtracellularSequence analysis7
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 115CytoplasmicSequence analysisAdd BLAST30
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 155ExtracellularSequence analysisAdd BLAST19
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Topological domaini177 – 184CytoplasmicSequence analysis8
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 231ExtracellularSequence analysisAdd BLAST26
Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
Topological domaini253 – 323CytoplasmicSequence analysisAdd BLAST71

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi361
OpenTargetsiENSG00000171885
PharmGKBiPA24922

Chemistry databases

ChEMBLiCHEMBL5964

Polymorphism and mutation databases

BioMutaiAQP4
DMDMi2506859

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000639481 – 323Aquaporin-4Add BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111Phosphoserine; by PKGBy similarity1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei180Phosphoserine; by PKCBy similarity1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei276PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1
Modified residuei289PhosphothreonineBy similarity1
Modified residuei321PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP55087
PeptideAtlasiP55087
PRIDEiP55087
ProteomicsDBi56788
56789 [P55087-2]

PTM databases

iPTMnetiP55087
PhosphoSitePlusiP55087
SwissPalmiP55087

Expressioni

Tissue specificityi

Brain - muscle >> heart, kidney, lung, and trachea.

Gene expression databases

BgeeiENSG00000171885 Expressed in 154 organ(s), highest expression level in lateral globus pallidus
CleanExiHS_AQP4
ExpressionAtlasiP55087 baseline and differential
GenevisibleiP55087 HS

Organism-specific databases

HPAiCAB005079
CAB058689
HPA014784

Interactioni

Subunit structurei

Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CREB3O43889-23EBI-10104898,EBI-625022

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106857, 2 interactors
DIPiDIP-48842N
IntActiP55087, 3 interactors
STRINGi9606.ENSP00000372654

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP55087
SMRiP55087
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55087

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 99NPA 13
Motifi213 – 215NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223 Eukaryota
COG0580 LUCA
GeneTreeiENSGT00760000119223
HOGENOMiHOG000288286
HOVERGENiHBG000312
InParanoidiP55087
KOiK09866
OMAiTQPFWKA
OrthoDBiEOG091G166T
PhylomeDBiP55087
TreeFamiTF312940

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR034294 Aquaporin_transptr
IPR000425 MIP
IPR022357 MIP_CS
PANTHERiPTHR19139 PTHR19139, 1 hit
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
TIGRFAMsiTIGR00861 MIP, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 2 (identifier: P55087-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSDRPTARRW GKCGPLCTRE NIMVAFKGVW TQAFWKAVTA EFLAMLIFVL
60 70 80 90 100
LSLGSTINWG GTEKPLPVDM VLISLCFGLS IATMVQCFGH ISGGHINPAV
110 120 130 140 150
TVAMVCTRKI SIAKSVFYIA AQCLGAIIGA GILYLVTPPS VVGGLGVTMV
160 170 180 190 200
HGNLTAGHGL LVELIITFQL VFTIFASCDS KRTDVTGSIA LAIGFSVAIG
210 220 230 240 250
HLFAINYTGA SMNPARSFGP AVIMGNWENH WIYWVGPIIG AVLAGGLYEY
260 270 280 290 300
VFCPDVEFKR RFKEAFSKAA QQTKGSYMEV EDNRSQVETD DLILKPGVVH
310 320
VIDVDRGEEK KGKDQSGEVL SSV
Length:323
Mass (Da):34,830
Last modified:November 1, 1997 - v2
Checksum:i1A1600CF0DC11052
GO
Isoform 1 (identifier: P55087-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:301
Mass (Da):32,300
Checksum:iC91C3D006C1B75A0
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KRM4J3KRM4_HUMAN
Aquaporin-4
AQP4
185Annotation score:
H0Y3H5H0Y3H5_HUMAN
Aquaporin-4
AQP4
98Annotation score:

Sequence cautioni

The sequence AAC50284 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC52112 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti246G → A in AAC52112 (PubMed:7559426).Curated1
Sequence conflicti287 – 288VE → AK in AAC52112 (PubMed:7559426).Curated2
Sequence conflicti296P → L in AAC52112 (PubMed:7559426).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0032321 – 22Missing in isoform 1. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34846 mRNA Translation: AAC52112.1 Different initiation.
U34845 mRNA Translation: AAC50284.1 Different initiation.
D63412 mRNA Translation: BAA09715.1
U63622 mRNA Translation: AAB26957.1
U63623 mRNA Translation: AAB26958.1
AC018371 Genomic DNA No translation available.
BC022286 mRNA Translation: AAH22286.1
CCDSiCCDS11889.1 [P55087-1]
CCDS58617.1 [P55087-2]
PIRiI39178
RefSeqiNP_001641.1, NM_001650.5 [P55087-1]
NP_004019.1, NM_004028.4 [P55087-2]
UniGeneiHs.315369

Genome annotation databases

EnsembliENST00000383168; ENSP00000372654; ENSG00000171885 [P55087-1]
ENST00000440832; ENSP00000393121; ENSG00000171885 [P55087-2]
ENST00000581374; ENSP00000462597; ENSG00000171885 [P55087-2]
GeneIDi361
KEGGihsa:361
UCSCiuc002kvz.4 human [P55087-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34846 mRNA Translation: AAC52112.1 Different initiation.
U34845 mRNA Translation: AAC50284.1 Different initiation.
D63412 mRNA Translation: BAA09715.1
U63622 mRNA Translation: AAB26957.1
U63623 mRNA Translation: AAB26958.1
AC018371 Genomic DNA No translation available.
BC022286 mRNA Translation: AAH22286.1
CCDSiCCDS11889.1 [P55087-1]
CCDS58617.1 [P55087-2]
PIRiI39178
RefSeqiNP_001641.1, NM_001650.5 [P55087-1]
NP_004019.1, NM_004028.4 [P55087-2]
UniGeneiHs.315369

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GD8X-ray1.80A32-254[»]
ProteinModelPortaliP55087
SMRiP55087
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106857, 2 interactors
DIPiDIP-48842N
IntActiP55087, 3 interactors
STRINGi9606.ENSP00000372654

Chemistry databases

ChEMBLiCHEMBL5964

Protein family/group databases

TCDBi1.A.8.8.5 the major intrinsic protein (mip) family

PTM databases

iPTMnetiP55087
PhosphoSitePlusiP55087
SwissPalmiP55087

Polymorphism and mutation databases

BioMutaiAQP4
DMDMi2506859

Proteomic databases

PaxDbiP55087
PeptideAtlasiP55087
PRIDEiP55087
ProteomicsDBi56788
56789 [P55087-2]

Protocols and materials databases

DNASUi361
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000383168; ENSP00000372654; ENSG00000171885 [P55087-1]
ENST00000440832; ENSP00000393121; ENSG00000171885 [P55087-2]
ENST00000581374; ENSP00000462597; ENSG00000171885 [P55087-2]
GeneIDi361
KEGGihsa:361
UCSCiuc002kvz.4 human [P55087-1]

Organism-specific databases

CTDi361
DisGeNETi361
EuPathDBiHostDB:ENSG00000171885.13
GeneCardsiAQP4
HGNCiHGNC:637 AQP4
HPAiCAB005079
CAB058689
HPA014784
MIMi600308 gene
neXtProtiNX_P55087
OpenTargetsiENSG00000171885
PharmGKBiPA24922
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0223 Eukaryota
COG0580 LUCA
GeneTreeiENSGT00760000119223
HOGENOMiHOG000288286
HOVERGENiHBG000312
InParanoidiP55087
KOiK09866
OMAiTQPFWKA
OrthoDBiEOG091G166T
PhylomeDBiP55087
TreeFamiTF312940

Enzyme and pathway databases

ReactomeiR-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-432047 Passive transport by Aquaporins
SIGNORiP55087

Miscellaneous databases

ChiTaRSiAQP4 human
EvolutionaryTraceiP55087
GeneWikiiAquaporin_4
GenomeRNAii361
PROiPR:P55087
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171885 Expressed in 154 organ(s), highest expression level in lateral globus pallidus
CleanExiHS_AQP4
ExpressionAtlasiP55087 baseline and differential
GenevisibleiP55087 HS

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR034294 Aquaporin_transptr
IPR000425 MIP
IPR022357 MIP_CS
PANTHERiPTHR19139 PTHR19139, 1 hit
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
TIGRFAMsiTIGR00861 MIP, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAQP4_HUMAN
AccessioniPrimary (citable) accession number: P55087
Secondary accession number(s): P78564
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: September 12, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again