UniProtKB - P55068 (PGCB_RAT)
Protein
Brevican core protein
Gene
Bcan
Organism
Rattus norvegicus (Rat)
Status
Functioni
May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Isoform 2 may function as a chondroitin sulfate-bearing cell surface receptor.
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- hyaluronic acid binding Source: UniProtKB-KW
GO - Biological processi
- brain development Source: RGD
- cell-matrix adhesion Source: RGD
- central nervous system development Source: GO_Central
- gliogenesis Source: RGD
- hippocampus development Source: RGD
- neuron projection extension Source: RGD
- regulation of neuronal synaptic plasticity Source: RGD
- skeletal system development Source: GO_Central
- synapse maturation Source: RGD
Keywordsi
Ligand | Hyaluronic acid, Lectin |
Enzyme and pathway databases
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix R-RNO-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-RNO-2022870, Chondroitin sulfate biosynthesis R-RNO-2022923, Dermatan sulfate biosynthesis R-RNO-2024101, CS/DS degradation R-RNO-3000178, ECM proteoglycans |
Names & Taxonomyi
Protein namesi | Recommended name: Brevican core proteinAlternative name(s): Brain-enriched hyaluronan-binding protein Short name: BEHAB |
Gene namesi | Name:Bcan Synonyms:Behab |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2194, Bcan |
Subcellular locationi
Extracellular region or secreted
Other locations
Extracellular region or secreted
- extracellular space Source: RGD
Other locations
- anchored component of membrane Source: RGD
- axon Source: RGD
- axon initial segment Source: RGD
- cell surface Source: RGD
- dendrite Source: RGD
- extracellular matrix Source: RGD
- GABA-ergic synapse Source: SynGO
- glutamatergic synapse Source: SynGO
- node of Ranvier Source: RGD
- perineuronal net Source: RGD
- perisynaptic extracellular matrix Source: SynGO
- synapse Source: RGD
Keywords - Cellular componenti
Extracellular matrix, Membrane, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000017513 | 23 – 883 | Brevican core proteinAdd BLAST | 861 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Disulfide bondi | 56 ↔ 136 | By similarity | |||
Glycosylationi | 129 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Disulfide bondi | 178 ↔ 249 | By similarity | |||
Disulfide bondi | 202 ↔ 223 | By similarity | |||
Disulfide bondi | 276 ↔ 351 | By similarity | |||
Disulfide bondi | 300 ↔ 321 | By similarity | |||
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | ||
Modified residuei | 413 | PhosphoserineCombined sources | 1 | ||
Disulfide bondi | 626 ↔ 637 | By similarity | |||
Disulfide bondi | 631 ↔ 646 | By similarity | |||
Disulfide bondi | 648 ↔ 657 | By similarity | |||
Disulfide bondi | 692 ↔ 784 | By similarity | |||
Disulfide bondi | 760 ↔ 776 | By similarity | |||
Disulfide bondi | 791 ↔ 834 | By similarity | |||
Disulfide bondi | 820 ↔ 847 | By similarity | |||
Isoform 2 (identifier: P55068-2) | |||||
Lipidationi | 622 | GPI-anchor amidated serine1 Publication | 1 |
Post-translational modificationi
Contains mostly chondroitin sulfate.
Keywords - PTMi
Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein, ProteoglycanProteomic databases
PaxDbi | P55068 |
PRIDEi | P55068 |
PTM databases
GlyGeni | P55068, 2 sites |
iPTMneti | P55068 |
PhosphoSitePlusi | P55068 |
SwissPalmi | P55068 |
Expressioni
Tissue specificityi
Brain.
Developmental stagei
Isoform 1 increases from day P4 to P64. Isoform 2 increases after day P8.
Interactioni
Subunit structurei
Interacts with TNR.
1 PublicationProtein-protein interaction databases
BioGRIDi | 247431, 2 interactors |
IntActi | P55068, 1 interactor |
STRINGi | 10116.ENSRNOP00000025496 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 154 | Ig-like V-typeAdd BLAST | 120 | |
Domaini | 156 – 251 | Link 1PROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 256 – 353 | Link 2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 622 – 658 | EGF-likePROSITE-ProRule annotationAdd BLAST | 37 | |
Domaini | 658 – 786 | C-type lectinPROSITE-ProRule annotationAdd BLAST | 129 | |
Domaini | 789 – 849 | SushiPROSITE-ProRule annotationAdd BLAST | 61 |
Sequence similaritiesi
Belongs to the aggrecan/versican proteoglycan family.Curated
Keywords - Domaini
EGF-like domain, Immunoglobulin domain, Repeat, Signal, SushiPhylogenomic databases
eggNOGi | KOG4297, Eukaryota |
InParanoidi | P55068 |
PhylomeDBi | P55068 |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.10.100.10, 3 hits |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR000742, EGF-like_dom IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003599, Ig_sub IPR013106, Ig_V-set IPR000538, Link_dom IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 1 hit PF07686, V-set, 1 hit PF00193, Xlink, 2 hits |
PRINTSi | PR01265, LINKMODULE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00409, IG, 1 hit SM00406, IGv, 1 hit SM00445, LINK, 2 hits |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF56436, SSF56436, 3 hits SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit PS01241, LINK_1, 2 hits PS50963, LINK_2, 2 hits PS50923, SUSHI, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P55068-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIPLLLSLLA ALVLTQAPAA LADDLKEDSS EDRAFRVRIG AAQLRGVLGG
60 70 80 90 100
ALAIPCHVHH LRPPPSRRAA PGFPRVKWTF LSGDREVEVL VARGLRVKVN
110 120 130 140 150
EAYRFRVALP AYPASLTDVS LVLSELRPND SGVYRCEVQH GIDDSSDAVE
160 170 180 190 200
VKVKGVVFLY REGSARYAFS FAGAQEACAR IGARIATPEQ LYAAYLGGYE
210 220 230 240 250
QCDAGWLSDQ TVRYPIQNPR EACYGDMDGY PGVRNYGVVG PDDLYDVYCY
260 270 280 290 300
AEDLNGELFL GAPPGKLTWE EARDYCLERG AQIASTGQLY AAWNGGLDRC
310 320 330 340 350
SPGWLADGSV RYPIITPSQR CGGGLPGVKT LFLFPNQTGF PSKQNRFNVY
360 370 380 390 400
CFRDSAHPSA FSEASSPASD GLEAIVTVTE KLEELQLPQE AVESESRGAI
410 420 430 440 450
YSIPITEDGG GGSSTPEDPA EAPRTPLESE TQSVAPPTGS SEEEGEALEE
460 470 480 490 500
EERFKDTETP KEEKEQENLW VWPTELSSPL PTGLETEHSL SQVSPPAQAV
510 520 530 540 550
LQVGASPSPR PPRVHGPTVE TLQPPGEGSL TSTPDGAREV GGETGSPELS
560 570 580 590 600
GVPREREEAG SSSLEDGPSL LPETWAPVGT REVETPSEEK SGRTVLTGTS
610 620 630 640 650
VQAQPVLPTD SASRGGVAVA PSSGDCIPSP CHNGGTCLEE KEGFRCLCVP
660 670 680 690 700
GYGGDLCDVG LHFCSPGWEP FQGACYKHFS TRRSWEEAES QCRALGAHLT
710 720 730 740 750
SICTPEEQDF VNDRYREYQW IGLNDRTIEG DFLWSDGPPL LYENWNPGQP
760 770 780 790 800
DSYFLSGENC VVMVWHDQGQ WSDVPCNYHL SYTCKMGLVS CGPPPQLPLA
810 820 830 840 850
QIFGRPRLRY AVDTVLRYRC RDGLAQRNLP LIRCQENGLW EAPQISCVPR
860 870 880
RPARALRSMT APEGPRGQLP RQRKALLTPP SSL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V8G4 | G3V8G4_RAT | Brevican core protein | Bcan LOC100910284, rCG_62833 | 883 | Annotation score: | ||
A0A0G2JYH8 | A0A0G2JYH8_RAT | Brevican core protein | Bcan | 371 | Annotation score: |
Sequence cautioni
The sequence CAA82215 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 – 52 | AL → WV in CAA82215 (PubMed:7512973).Curated | 2 | |
Sequence conflicti | 503 | V → L in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 518 – 519 | TV → PA in AAA87847 (PubMed:7488217).Curated | 2 | |
Sequence conflicti | 526 | G → R in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 541 | G → A in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 556 | R → S in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 573 | E → A in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 583 | V → L in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 649 | V → L in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 670 | P → A in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 738 | P → A in AAA87847 (PubMed:7488217).Curated | 1 | |
Sequence conflicti | 809 | R → A in AAA87847 (PubMed:7488217).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003076 | 625 – 645 | DCIPS…KEGFR → NSAEGSMPAFLLFLLLQLWD T in isoform 2. CuratedAdd BLAST | 21 | |
Alternative sequenceiVSP_003077 | 646 – 883 | Missing in isoform 2. CuratedAdd BLAST | 238 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79881 mRNA Translation: CAA56255.1 X86406 mRNA Translation: CAA60160.1 U37142 mRNA Translation: AAA87847.1 Z28366 mRNA Translation: CAA82215.1 Frameshift. |
PIRi | A53908 S49126 |
RefSeqi | NP_001028837.1, NM_001033665.1 NP_037048.2, NM_012916.2 |
Genome annotation databases
GeneIDi | 25393 |
KEGGi | rno:25393 |
UCSCi | RGD:2194, rat [P55068-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X79881 mRNA Translation: CAA56255.1 X86406 mRNA Translation: CAA60160.1 U37142 mRNA Translation: AAA87847.1 Z28366 mRNA Translation: CAA82215.1 Frameshift. |
PIRi | A53908 S49126 |
RefSeqi | NP_001028837.1, NM_001033665.1 NP_037048.2, NM_012916.2 |
3D structure databases
SMRi | P55068 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247431, 2 interactors |
IntActi | P55068, 1 interactor |
STRINGi | 10116.ENSRNOP00000025496 |
PTM databases
GlyGeni | P55068, 2 sites |
iPTMneti | P55068 |
PhosphoSitePlusi | P55068 |
SwissPalmi | P55068 |
Proteomic databases
PaxDbi | P55068 |
PRIDEi | P55068 |
Protocols and materials databases
ABCDi | P55068, 2 sequenced antibodies |
Genome annotation databases
GeneIDi | 25393 |
KEGGi | rno:25393 |
UCSCi | RGD:2194, rat [P55068-1] |
Organism-specific databases
CTDi | 63827 |
RGDi | 2194, Bcan |
Phylogenomic databases
eggNOGi | KOG4297, Eukaryota |
InParanoidi | P55068 |
PhylomeDBi | P55068 |
Enzyme and pathway databases
Reactomei | R-RNO-1474228, Degradation of the extracellular matrix R-RNO-1971475, A tetrasaccharide linker sequence is required for GAG synthesis R-RNO-2022870, Chondroitin sulfate biosynthesis R-RNO-2022923, Dermatan sulfate biosynthesis R-RNO-2024101, CS/DS degradation R-RNO-3000178, ECM proteoglycans |
Miscellaneous databases
PROi | PR:P55068 |
Family and domain databases
CDDi | cd00033, CCP, 1 hit |
Gene3Di | 2.60.40.10, 1 hit 3.10.100.10, 3 hits |
InterProi | View protein in InterPro IPR001304, C-type_lectin-like IPR016186, C-type_lectin-like/link_sf IPR018378, C-type_lectin_CS IPR016187, CTDL_fold IPR000742, EGF-like_dom IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003006, Ig/MHC_CS IPR003599, Ig_sub IPR013106, Ig_V-set IPR000538, Link_dom IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom |
Pfami | View protein in Pfam PF00008, EGF, 1 hit PF00059, Lectin_C, 1 hit PF00084, Sushi, 1 hit PF07686, V-set, 1 hit PF00193, Xlink, 2 hits |
PRINTSi | PR01265, LINKMODULE |
SMARTi | View protein in SMART SM00032, CCP, 1 hit SM00034, CLECT, 1 hit SM00181, EGF, 1 hit SM00409, IG, 1 hit SM00406, IGv, 1 hit SM00445, LINK, 2 hits |
SUPFAMi | SSF48726, SSF48726, 1 hit SSF56436, SSF56436, 3 hits SSF57535, SSF57535, 1 hit |
PROSITEi | View protein in PROSITE PS00615, C_TYPE_LECTIN_1, 1 hit PS50041, C_TYPE_LECTIN_2, 1 hit PS00022, EGF_1, 1 hit PS01186, EGF_2, 1 hit PS50026, EGF_3, 1 hit PS50835, IG_LIKE, 1 hit PS00290, IG_MHC, 1 hit PS01241, LINK_1, 2 hits PS50963, LINK_2, 2 hits PS50923, SUSHI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PGCB_RAT | |
Accessioni | P55068Primary (citable) accession number: P55068 Secondary accession number(s): Q62860, Q63040, Q63513 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families