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UniProtKB - P55059 (PDI_HUMIN)
Protein
Protein disulfide-isomerase
Gene
N/A
Organism
Humicola insolens (Soft-rot fungus)
Status
Functioni
Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.
By similarityCatalytic activityi
- Catalyzes the rearrangement of -S-S- bonds in proteins. EC:5.3.4.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 50 | NucleophileBy similarity | 1 | |
Sitei | 51 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 52 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 53 | NucleophileBy similarity | 1 | |
Sitei | 113 | Lowers pKa of C-terminal Cys of first active siteBy similarity | 1 | |
Active sitei | 385 | NucleophileBy similarity | 1 | |
Sitei | 386 | Contributes to redox potential valueBy similarity | 1 | |
Sitei | 387 | Contributes to redox potential valueBy similarity | 1 | |
Active sitei | 388 | NucleophileBy similarity | 1 | |
Sitei | 451 | Lowers pKa of C-terminal Cys of second active siteBy similarity | 1 |
GO - Molecular functioni
- protein disulfide isomerase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Isomerase |
Enzyme and pathway databases
BRENDAi | 5.3.4.1, 2710 |
Names & Taxonomyi
Protein namesi | |
Organismi | Humicola insolens (Soft-rot fungus) |
Taxonomic identifieri | 34413 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Chaetomiaceae › Humicola |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulumPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Sequence analysisAdd BLAST | 20 | |
ChainiPRO_0000034215 | 21 – 505 | Protein disulfide-isomeraseAdd BLAST | 485 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 50 ↔ 53 | Redox-activePROSITE-ProRule annotation | ||
Disulfide bondi | 385 ↔ 388 | Redox-activePROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bondStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P55059 |
BMRBi | P55059 |
SMRi | P55059 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55059 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 128 | Thioredoxin 1PROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 335 – 465 | Thioredoxin 2PROSITE-ProRule annotationAdd BLAST | 131 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 470 – 505 | DisorderedSequence analysisAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 502 – 505 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 474 – 488 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 489 – 505 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the protein disulfide isomerase family.Curated
Keywords - Domaini
Redox-active center, Repeat, SignalFamily and domain databases
IDEALi | IID50214 |
InterProi | View protein in InterPro IPR005788, Disulphide_isomerase IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit TIGR01126, pdi_dom, 2 hits |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P55059-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHKAQKFALG LLAAAAVATA SDVVQLKKDT FDDFIKTNDL VLAEFFAPWC
60 70 80 90 100
GHCKALAPEY EEAATTLKEK NIKLAKVDCT EETDLCQQHG VEGYPTLKVF
110 120 130 140 150
RGLDNVSPYK GQRKAAAITS YMIKQSLPAV SEVTKDNLEE FKKADKAVLV
160 170 180 190 200
AYVDASDKAS SEVFTQVAEK LRDNYPFGSS SDAALAEAEG VKAPAIVLYK
210 220 230 240 250
DFDEGKAVFS EKFEVEAIEK FAKTGATPLI GEIGPETYSD YMSAGIPLAY
260 270 280 290 300
IFAETAEERK ELSDKLKPIA EAQRGVINFG TIDAKAFGAH AGNLNLKTDK
310 320 330 340 350
FPAFAIQEVA KNQKFPFDQE KEITFEAIKA FVDDFVAGKI EPSIKSEPIP
360 370 380 390 400
EKQEGPVTVV VAKNYNEIVL DDTKDVLIEF YAPWCGHCKA LAPKYEELGA
410 420 430 440 450
LYAKSEFKDR VVIAKVDATA NDVPDEIQGF PTIKLYPAGA KGQPVTYSGS
460 470 480 490 500
RTVEDLIKFI AENGKYKAAI SEDAEETSSA TETTTETATK SEEAAKETAT
EHDEL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S74296 mRNA Translation: AAC60578.1 |
PIRi | JC2291 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S74296 mRNA Translation: AAC60578.1 |
PIRi | JC2291 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DJJ | NMR | - | A | 354-469 | [»] | |
2DJK | NMR | - | A | 228-355 | [»] | |
2KP1 | NMR | - | A | 354-469 | [»] | |
2KP2 | NMR | - | A | 228-355 | [»] | |
2RUE | NMR | - | A | 354-469 | [»] | |
2RUF | NMR | - | A | 354-469 | [»] | |
3WT1 | X-ray | 1.85 | A/B/C/D | 228-469 | [»] | |
3WT2 | X-ray | 3.30 | A/B/C | 228-469 | [»] | |
5CRW | X-ray | 1.60 | A | 228-469 | [»] | |
AlphaFoldDBi | P55059 | |||||
BMRBi | P55059 | |||||
SMRi | P55059 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Enzyme and pathway databases
BRENDAi | 5.3.4.1, 2710 |
Miscellaneous databases
EvolutionaryTracei | P55059 |
Family and domain databases
IDEALi | IID50214 |
InterProi | View protein in InterPro IPR005788, Disulphide_isomerase IPR005792, Prot_disulphide_isomerase IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00085, Thioredoxin, 2 hits |
SUPFAMi | SSF52833, SSF52833, 4 hits |
TIGRFAMsi | TIGR01130, ER_PDI_fam, 1 hit TIGR01126, pdi_dom, 2 hits |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit PS00194, THIOREDOXIN_1, 2 hits PS51352, THIOREDOXIN_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PDI_HUMIN | |
Accessioni | P55059Primary (citable) accession number: P55059 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families