UniProtKB - P55042 (RAD_HUMAN)
GTP-binding protein RAD
RRAD
Functioni
May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity).
Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 98 – 105 | GTPBy similarity | 8 | |
Nucleotide bindingi | 203 – 206 | GTPBy similarity | 4 |
GO - Molecular functioni
- calcium channel regulator activity Source: GO_Central
- calmodulin binding Source: UniProtKB-KW
- GTPase activity Source: ProtInc
- GTP binding Source: GO_Central
GO - Biological processi
- negative regulation of high voltage-gated calcium channel activity Source: InterPro
- small GTPase mediated signal transduction Source: ProtInc
Keywordsi
Molecular function | Calmodulin-binding |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P55042 |
Reactomei | R-HSA-9031628, NGF-stimulated transcription |
SignaLinki | P55042 |
SIGNORi | P55042 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding protein RADAlternative name(s): RAD1 Ras associated with diabetes |
Gene namesi | Name:RRAD Synonyms:RAD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10446, RRAD |
MIMi | 179503, gene |
neXtProti | NX_P55042 |
VEuPathDBi | HostDB:ENSG00000166592 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytosol
- cytosol Source: Reactome
Plasma Membrane
- plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 6236 |
OpenTargetsi | ENSG00000166592 |
PharmGKBi | PA34860 |
Miscellaneous databases
Pharosi | P55042, Tbio |
Genetic variation databases
BioMutai | RRAD |
DMDMi | 38258885 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122478 | 1 – 308 | GTP-binding protein RADAdd BLAST | 308 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | P55042 |
MassIVEi | P55042 |
MaxQBi | P55042 |
PaxDbi | P55042 |
PeptideAtlasi | P55042 |
PRIDEi | P55042 |
ProteomicsDBi | 56766 |
TopDownProteomicsi | P55042 |
PTM databases
iPTMneti | P55042 |
PhosphoSitePlusi | P55042 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000166592, Expressed in quadriceps femoris and 186 other tissues |
ExpressionAtlasi | P55042, baseline and differential |
Genevisiblei | P55042, HS |
Organism-specific databases
HPAi | ENSG00000166592, Group enriched (heart muscle, skeletal muscle) |
Interactioni
Subunit structurei
Interacts with calmodulin preferentially in the inactive, GDP-bound form. Binds CAMKII which is capable of phosphorylating RAD in vitro.
Interacts with CAMK2D and CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane (By similarity).
By similarityBinary interactionsi
P55042
With | #Exp. | IntAct |
---|---|---|
CCNDBP1 [O95273] | 5 | EBI-3911502,EBI-748961 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 112150, 18 interactors |
IntActi | P55042, 3 interactors |
STRINGi | 9606.ENSP00000299759 |
Miscellaneous databases
RNActi | P55042, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P55042 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55042 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 88 | DisorderedSequence analysisAdd BLAST | 88 | |
Regioni | 278 – 297 | Calmodulin-bindingAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000159836 |
HOGENOMi | CLU_041217_3_2_1 |
InParanoidi | P55042 |
OMAi | MPQTGQT |
OrthoDBi | 679855at2759 |
PhylomeDBi | P55042 |
TreeFami | TF314379 |
Family and domain databases
DisProti | DP02572 |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028869, RAD IPR017358, RGK IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
PANTHERi | PTHR45775:SF3, PTHR45775:SF3, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTLNGGGSGA GGSRGGGQER ERRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LTPGALTAAA AGTGTQGPRL DWPEDSEDSL SSGGSDSDES VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGVEDGPEA EAAGHTYDRS IVVDGEEASL MVYDIWEQDG
160 170 180 190 200
GRWLPGHCMA MGDAYVIVYS VTDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKEA NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK
SCHDLSVL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3KSM6 | J3KSM6_HUMAN | GTP-binding protein RAD | RRAD | 162 | Annotation score: | ||
J3KRG9 | J3KRG9_HUMAN | GTP-binding protein RAD | RRAD | 136 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_049497 | 66 | Q → P. Corresponds to variant dbSNP:rs7198458Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24564 mRNA Translation: AAA36540.1 BC011645 mRNA Translation: AAH11645.1 BC057815 mRNA Translation: AAH57815.1 |
CCDSi | CCDS10824.1 |
PIRi | A49334 |
RefSeqi | NP_001122322.1, NM_001128850.1 NP_004156.1, NM_004165.2 |
Genome annotation databases
Ensembli | ENST00000299759; ENSP00000299759; ENSG00000166592 ENST00000420652; ENSP00000388744; ENSG00000166592 |
GeneIDi | 6236 |
KEGGi | hsa:6236 |
MANE-Selecti | ENST00000299759.11; ENSP00000299759.6; NM_004165.3; NP_004156.1 |
UCSCi | uc002eqn.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L24564 mRNA Translation: AAA36540.1 BC011645 mRNA Translation: AAH11645.1 BC057815 mRNA Translation: AAH57815.1 |
CCDSi | CCDS10824.1 |
PIRi | A49334 |
RefSeqi | NP_001122322.1, NM_001128850.1 NP_004156.1, NM_004165.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DPX | X-ray | 1.80 | A/B | 87-258 | [»] | |
2GJS | X-ray | 1.90 | A/B | 87-260 | [»] | |
3Q72 | X-ray | 1.66 | A/B | 90-255 | [»] | |
3Q7P | X-ray | 2.50 | A/B | 90-255 | [»] | |
3Q7Q | X-ray | 2.30 | A/B | 90-255 | [»] | |
SMRi | P55042 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 112150, 18 interactors |
IntActi | P55042, 3 interactors |
STRINGi | 9606.ENSP00000299759 |
PTM databases
iPTMneti | P55042 |
PhosphoSitePlusi | P55042 |
Genetic variation databases
BioMutai | RRAD |
DMDMi | 38258885 |
Proteomic databases
jPOSTi | P55042 |
MassIVEi | P55042 |
MaxQBi | P55042 |
PaxDbi | P55042 |
PeptideAtlasi | P55042 |
PRIDEi | P55042 |
ProteomicsDBi | 56766 |
TopDownProteomicsi | P55042 |
Protocols and materials databases
Antibodypediai | 44221, 171 antibodies from 32 providers |
DNASUi | 6236 |
Genome annotation databases
Ensembli | ENST00000299759; ENSP00000299759; ENSG00000166592 ENST00000420652; ENSP00000388744; ENSG00000166592 |
GeneIDi | 6236 |
KEGGi | hsa:6236 |
MANE-Selecti | ENST00000299759.11; ENSP00000299759.6; NM_004165.3; NP_004156.1 |
UCSCi | uc002eqn.2, human |
Organism-specific databases
CTDi | 6236 |
DisGeNETi | 6236 |
GeneCardsi | RRAD |
HGNCi | HGNC:10446, RRAD |
HPAi | ENSG00000166592, Group enriched (heart muscle, skeletal muscle) |
MIMi | 179503, gene |
neXtProti | NX_P55042 |
OpenTargetsi | ENSG00000166592 |
PharmGKBi | PA34860 |
VEuPathDBi | HostDB:ENSG00000166592 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000159836 |
HOGENOMi | CLU_041217_3_2_1 |
InParanoidi | P55042 |
OMAi | MPQTGQT |
OrthoDBi | 679855at2759 |
PhylomeDBi | P55042 |
TreeFami | TF314379 |
Enzyme and pathway databases
PathwayCommonsi | P55042 |
Reactomei | R-HSA-9031628, NGF-stimulated transcription |
SignaLinki | P55042 |
SIGNORi | P55042 |
Miscellaneous databases
BioGRID-ORCSi | 6236, 20 hits in 1050 CRISPR screens |
EvolutionaryTracei | P55042 |
GeneWikii | RRAD |
GenomeRNAii | 6236 |
Pharosi | P55042, Tbio |
PROi | PR:P55042 |
RNActi | P55042, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166592, Expressed in quadriceps femoris and 186 other tissues |
ExpressionAtlasi | P55042, baseline and differential |
Genevisiblei | P55042, HS |
Family and domain databases
DisProti | DP02572 |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028869, RAD IPR017358, RGK IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
PANTHERi | PTHR45775:SF3, PTHR45775:SF3, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD_HUMAN | |
Accessioni | P55042Primary (citable) accession number: P55042 Secondary accession number(s): Q96F39 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 7, 2003 | |
Last modified: | February 23, 2022 | |
This is version 179 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families