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Protein

GTP-binding protein GEM

Gene

Gem

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi81 – 88GTPBy similarity8
Nucleotide bindingi190 – 193GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein GEM
Alternative name(s):
GTP-binding mitogen-induced T-cell protein
RAS-like protein KIR
Gene namesi
Name:Gem
Synonyms:Kir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:99844 Gem

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi269W → G: No calmodulin binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224761 – 295GTP-binding protein GEMAdd BLAST295

Post-translational modificationi

Phosphorylated on tyrosine residues.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP55041
PaxDbiP55041
PeptideAtlasiP55041
PRIDEiP55041

PTM databases

iPTMnetiP55041
PhosphoSitePlusiP55041

Expressioni

Inductioni

By mitogens.

Gene expression databases

BgeeiENSMUSG00000028214 Expressed in 170 organ(s), highest expression level in islet of Langerhans
ExpressionAtlasiP55041 baseline and differential
GenevisibleiP55041 MM

Interactioni

Subunit structurei

Interacts with calmodulin in a Ca2+-dependent manner. Calmodulin binding significantly decreases GTP binding. Binds ROCK1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
YWHABP319463EBI-7082069,EBI-359815From Homo sapiens.

Protein-protein interaction databases

BioGridi199898, 1 interactor
IntActiP55041, 2 interactors
MINTiP55041
STRINGi10090.ENSMUSP00000029868

Structurei

3D structure databases

ProteinModelPortaliP55041
SMRiP55041
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 284Calmodulin-bindingAdd BLAST20

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00860000133672
HOGENOMiHOG000246961
HOVERGENiHBG104899
InParanoidiP55041
KOiK07846
OMAiEDPCRRS
OrthoDBiEOG091G0OCN
PhylomeDBiP55041
TreeFamiTF314379

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR017358 RGK
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PIRSFiPIRSF038017 GTP-binding_GEM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequencei

Sequence statusi: Complete.

P55041-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLNNVTMRQ GTVGMQPQQR WSIPADARHL MVQKDPHPCN LRNRHSTAPE
60 70 80 90 100
EHCRRSWSSD STDSVISSES GNTYYRVVLI GEQGVGKSTL ANIFAGVHDS
110 120 130 140 150
MDSDCEVLGE DTYERTLVVD GESATIILLD MWENKGENEW LHDHCMQVGD
160 170 180 190 200
AYLIVYSITD RASFEKASEL RIQLRRARQT EDIPIILVGN KSDLVRCREV
210 220 230 240 250
SVSEGRACAV VFDCKFIETS AAVQHNVKEL FEGIVRQVRL RRDSKEKNER
260 270 280 290
RLAYQKRRES IPRKARRFWG KIVAKNNKNM AFKLKSKSCH DLSVL
Length:295
Mass (Da):33,725
Last modified:November 7, 2003 - v2
Checksum:i4618F225D4FAAFA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23I → M in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti56S → T in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti235V → E in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti241R → P in AAA64912 (PubMed:7912851).Curated1
Sequence conflicti282 – 284FKL → SSS in AAA64912 (PubMed:7912851).Curated3
Sequence conflicti294V → W in AAC52145 (PubMed:7809057).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10551 mRNA Translation: AAA64912.1
U13053 mRNA Translation: AAC52145.1
BC029668 mRNA Translation: AAH29668.1
CCDSiCCDS17971.1
PIRiB54575
I49117
RefSeqiNP_034406.2, NM_010276.4
UniGeneiMm.247486

Genome annotation databases

EnsembliENSMUST00000029868; ENSMUSP00000029868; ENSMUSG00000028214
ENSMUST00000108304; ENSMUSP00000103939; ENSMUSG00000028214
GeneIDi14579
KEGGimmu:14579
UCSCiuc008rzv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiGEM_MOUSE
AccessioniPrimary (citable) accession number: P55041
Secondary accession number(s): Q8JZS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 7, 2003
Last modified: September 12, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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