UniProtKB - P55040 (GEM_HUMAN)
Protein
GTP-binding protein GEM
Gene
GEM
Organism
Homo sapiens (Human)
Status
Functioni
Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 82 – 89 | GTPBy similarity | 8 | |
Nucleotide bindingi | 191 – 194 | GTPBy similarity | 4 |
GO - Molecular functioni
- calcium channel regulator activity Source: GO_Central
- calmodulin binding Source: UniProtKB-KW
- GDP binding Source: BHF-UCL
- GTPase activity Source: BHF-UCL
- GTP binding Source: BHF-UCL
- magnesium ion binding Source: BHF-UCL
GO - Biological processi
- cell surface receptor signaling pathway Source: ProtInc
- chromosome organization Source: UniProtKB
- immune response Source: ProtInc
- metaphase plate congression Source: UniProtKB
- mitotic cell cycle Source: UniProtKB
- negative regulation of high voltage-gated calcium channel activity Source: InterPro
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Calmodulin-binding |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P55040 |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding protein GEMAlternative name(s): GTP-binding mitogen-induced T-cell protein RAS-like protein KIR |
Gene namesi | Name:GEM Synonyms:KIR |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000164949.7 |
HGNCi | HGNC:4234, GEM |
MIMi | 600164, gene |
neXtProti | NX_P55040 |
Subcellular locationi
Plasma membrane
Cytoskeleton
- mitotic spindle Source: UniProtKB
- spindle midzone Source: UniProtKB
Nucleus
- nucleus Source: Ensembl
Plasma Membrane
- cytoplasmic side of plasma membrane Source: BHF-UCL
- plasma membrane Source: GO_Central
Other locations
- midbody Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 2669 |
OpenTargetsi | ENSG00000164949 |
PharmGKBi | PA28645 |
Miscellaneous databases
Pharosi | P55040, Tbio |
Polymorphism and mutation databases
BioMutai | GEM |
DMDMi | 1707896 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122475 | 1 – 296 | GTP-binding protein GEMAdd BLAST | 296 |
Post-translational modificationi
Phosphorylated on tyrosine residues.
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P55040 |
jPOSTi | P55040 |
MassIVEi | P55040 |
MaxQBi | P55040 |
PaxDbi | P55040 |
PeptideAtlasi | P55040 |
PRIDEi | P55040 |
ProteomicsDBi | 56765 |
PTM databases
iPTMneti | P55040 |
PhosphoSitePlusi | P55040 |
Expressioni
Tissue specificityi
Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.
Inductioni
By mitogens.
Gene expression databases
Bgeei | ENSG00000164949, Expressed in pigmented layer of retina and 223 other tissues |
ExpressionAtlasi | P55040, baseline and differential |
Genevisiblei | P55040, HS |
Organism-specific databases
HPAi | ENSG00000164949, Tissue enhanced (gallbladder) |
Interactioni
Subunit structurei
Interacts with calmodulin in a Ca2+-dependent manner. Binds ROCK1.
2 PublicationsBinary interactionsi
P55040
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 108937, 144 interactors |
DIPi | DIP-41587N |
IntActi | P55040, 148 interactors |
MINTi | P55040 |
STRINGi | 9606.ENSP00000297596 |
Miscellaneous databases
RNActi | P55040, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P55040 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P55040 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 266 – 285 | Calmodulin-bindingBy similarityAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000157830 |
HOGENOMi | CLU_041217_3_2_1 |
InParanoidi | P55040 |
OMAi | QTELHRW |
OrthoDBi | 679855at2759 |
PhylomeDBi | P55040 |
TreeFami | TF314379 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR017358, RGK IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P55040-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLNNVTMRQ GTVGMQPQQQ RWSIPADGRH LMVQKEPHQY SHRNRHSATP
60 70 80 90 100
EDHCRRSWSS DSTDSVISSE SGNTYYRVVL IGEQGVGKST LANIFAGVHD
110 120 130 140 150
SMDSDCEVLG EDTYERTLMV DGESATIILL DMWENKGENE WLHDHCMQVG
160 170 180 190 200
DAYLIVYSIT DRASFEKASE LRIQLRRARQ TEDIPIILVG NKSDLVRCRE
210 220 230 240 250
VSVSEGRACA VVFDCKFIET SAAVQHNVKE LFEGIVRQVR LRRDSKEKNE
260 270 280 290
RRLAYQKRKE SMPRKARRFW GKIVAKNNKN MAFKLKSKSC HDLSVL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE5RJF9 | E5RJF9_HUMAN | GTP-binding protein GEM | GEM | 93 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 | I → V in AAC50067 (PubMed:7809057).Curated | 1 | |
Sequence conflicti | 174 – 175 | QL → HV in AAC50067 (PubMed:7809057).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_020097 | 43 | R → G. Corresponds to variant dbSNP:rs2170363Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10550 mRNA Translation: AAA64911.1 U13052 mRNA Translation: AAC50067.1 AK314017 mRNA Translation: BAG36728.1 CH471060 Genomic DNA Translation: EAW91709.1 BC022010 mRNA Translation: AAH22010.1 |
CCDSi | CCDS6261.1 |
PIRi | A54575 I38745 |
RefSeqi | NP_005252.1, NM_005261.3 NP_859053.1, NM_181702.2 |
Genome annotation databases
Ensembli | ENST00000297596; ENSP00000297596; ENSG00000164949 ENST00000396194; ENSP00000379497; ENSG00000164949 |
GeneIDi | 2669 |
KEGGi | hsa:2669 |
UCSCi | uc003ygi.4, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10550 mRNA Translation: AAA64911.1 U13052 mRNA Translation: AAC50067.1 AK314017 mRNA Translation: BAG36728.1 CH471060 Genomic DNA Translation: EAW91709.1 BC022010 mRNA Translation: AAH22010.1 |
CCDSi | CCDS6261.1 |
PIRi | A54575 I38745 |
RefSeqi | NP_005252.1, NM_005261.3 NP_859053.1, NM_181702.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2CJW | X-ray | 2.10 | A/B | 74-261 | [»] | |
2G3Y | X-ray | 2.40 | A | 62-249 | [»] | |
2HT6 | X-ray | 2.40 | A/B | 71-243 | [»] | |
SMRi | P55040 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108937, 144 interactors |
DIPi | DIP-41587N |
IntActi | P55040, 148 interactors |
MINTi | P55040 |
STRINGi | 9606.ENSP00000297596 |
PTM databases
iPTMneti | P55040 |
PhosphoSitePlusi | P55040 |
Polymorphism and mutation databases
BioMutai | GEM |
DMDMi | 1707896 |
Proteomic databases
EPDi | P55040 |
jPOSTi | P55040 |
MassIVEi | P55040 |
MaxQBi | P55040 |
PaxDbi | P55040 |
PeptideAtlasi | P55040 |
PRIDEi | P55040 |
ProteomicsDBi | 56765 |
Protocols and materials databases
Antibodypediai | 12852, 168 antibodies |
DNASUi | 2669 |
Genome annotation databases
Ensembli | ENST00000297596; ENSP00000297596; ENSG00000164949 ENST00000396194; ENSP00000379497; ENSG00000164949 |
GeneIDi | 2669 |
KEGGi | hsa:2669 |
UCSCi | uc003ygi.4, human |
Organism-specific databases
CTDi | 2669 |
DisGeNETi | 2669 |
EuPathDBi | HostDB:ENSG00000164949.7 |
GeneCardsi | GEM |
HGNCi | HGNC:4234, GEM |
HPAi | ENSG00000164949, Tissue enhanced (gallbladder) |
MIMi | 600164, gene |
neXtProti | NX_P55040 |
OpenTargetsi | ENSG00000164949 |
PharmGKBi | PA28645 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000157830 |
HOGENOMi | CLU_041217_3_2_1 |
InParanoidi | P55040 |
OMAi | QTELHRW |
OrthoDBi | 679855at2759 |
PhylomeDBi | P55040 |
TreeFami | TF314379 |
Enzyme and pathway databases
PathwayCommonsi | P55040 |
Miscellaneous databases
BioGRID-ORCSi | 2669, 5 hits in 848 CRISPR screens |
ChiTaRSi | GEM, human |
EvolutionaryTracei | P55040 |
GeneWikii | GEM_(gene) |
GenomeRNAii | 2669 |
Pharosi | P55040, Tbio |
PROi | PR:P55040 |
RNActi | P55040, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164949, Expressed in pigmented layer of retina and 223 other tissues |
ExpressionAtlasi | P55040, baseline and differential |
Genevisiblei | P55040, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR017358, RGK IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GEM_HUMAN | |
Accessioni | P55040Primary (citable) accession number: P55040 Secondary accession number(s): B2RA31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations