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Protein

26S proteasome non-ATPase regulatory subunit 4

Gene

Rpn10

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds and presumably selects ubiquitin-conjugates for destruction.

GO - Molecular functioni

  • polyubiquitin modification-dependent protein binding Source: FlyBase
  • ubiquitin conjugating enzyme binding Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • mitotic sister chromatid segregation Source: FlyBase
  • proteasome assembly Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase

Enzyme and pathway databases

BRENDAi3.4.25.1 1994
ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-538848 Degradation of CLK
R-DME-538864 Degradation of CRY
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 4
Alternative name(s):
26S proteasome regulatory subunit RPN10
26S proteasome regulatory subunit S5A
54 kDa subunit of mu particle
Multiubiquitin chain-binding protein
p54
Gene namesi
Name:Rpn10
Synonyms:PROS-54, Pros54
ORF Names:CG7619
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0015283 Rpn10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738301 – 39626S proteasome non-ATPase regulatory subunit 4Add BLAST396

Proteomic databases

PaxDbiP55035
PRIDEiP55035

Expressioni

Gene expression databases

BgeeiFBgn0015283
ExpressionAtlasiP55035 baseline and differential
GenevisibleiP55035 DM

Interactioni

Subunit structurei

The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively (By similarity). Interacts with Ubc4.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi65632, 21 interactors
DIPiDIP-22777N
IntActiP55035, 19 interactors
MINTiP55035
STRINGi7227.FBpp0078024

Structurei

3D structure databases

ProteinModelPortaliP55035
SMRiP55035
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 188VWFAAdd BLAST188
Domaini212 – 231UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini276 – 295UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini303 – 322UIM 3PROSITE-ProRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the proteasome subunit S5A family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2884 Eukaryota
COG5148 LUCA
GeneTreeiENSGT00530000064050
InParanoidiP55035
KOiK03029
OMAiMGGKGPE
OrthoDBiEOG091G0A6L
PhylomeDBiP55035

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR003903 UIM_dom
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF02809 UIM, 3 hits
PF13519 VWA_2, 1 hit
SMARTiView protein in SMART
SM00726 UIM, 3 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 3 hits

Sequencei

Sequence statusi: Complete.

P55035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLESTMICF DNSDFQRNGD YFPTRLIVQR DGINLVCLTK LRSNPENNVG
60 70 80 90 100
LMTLSNTVEV LATLTSDAGR IFSKMHLVQP KGEINLLTGI RIAHLVLKHR
110 120 130 140 150
QGKNHKMRIV VFVGSPINHE EGDLVKQAKR LKKEKVNVDI VSFGDHGNNN
160 170 180 190 200
EILTAFINAL NGKDGTGSHL VSVPRGSVLS DALLSSPIIQ GEDGMGGAGL
210 220 230 240 250
GGNVFEFGVD PNEDPELALA LRVSMEEQRQ RQESEQRRAN PDGAPPTGGD
260 270 280 290 300
AGGGGGVSGS GPGNEESAGA ENEANTEEAM LQRALALSTE TPEDNLPDFA
310 320 330 340 350
NMTEEEQIAF AMQMSMQDAP DDSVTQQAKR PKTDEANAPM DVDEDYSEVI
360 370 380 390
GDPAFLQSVL ENLPGVDPQS EAVRDAVGSL NKDKDKKSDG KDSQKK
Length:396
Mass (Da):42,618
Last modified:November 2, 2001 - v2
Checksum:i061418F6F7FE819F
GO

Sequence cautioni

The sequence AAL90071 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9C → S in AAB35145 (PubMed:7649173).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S79502 Genomic DNA Translation: AAB35145.1
AE014296 Genomic DNA Translation: AAF51741.1
AY089333 mRNA Translation: AAL90071.2 Different initiation.
PIRiS66528
RefSeqiNP_001262168.1, NM_001275239.1
NP_524204.1, NM_079480.3
UniGeneiDm.23542

Genome annotation databases

EnsemblMetazoaiFBtr0078368; FBpp0078024; FBgn0015283
FBtr0333453; FBpp0305644; FBgn0015283
GeneIDi40388
KEGGidme:Dmel_CG7619

Similar proteinsi

Entry informationi

Entry nameiPSMD4_DROME
AccessioniPrimary (citable) accession number: P55035
Secondary accession number(s): Q86R87, Q9V473
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 2, 2001
Last modified: July 18, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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