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Protein

Allograft inflammatory factor 1

Gene

AIF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi58 – 691PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi94 – 1052PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActin-binding
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Allograft inflammatory factor 1
Short name:
AIF-1
Alternative name(s):
Ionized calcium-binding adapter molecule 1
Protein G1
Gene namesi
Name:AIF1
Synonyms:G1, IBA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204472.12
HGNCiHGNC:352 AIF1
MIMi601833 gene
neXtProtiNX_P55008

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi199
OpenTargetsiENSG00000204472
PharmGKBiPA24646

Polymorphism and mutation databases

BioMutaiAIF1
DMDMi1703217

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000738652 – 147Allograft inflammatory factor 1Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei11N6-acetyllysineCombined sources1
Modified residuei39PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP55008
PaxDbiP55008
PeptideAtlasiP55008
PRIDEiP55008
ProteomicsDBi56753
56754 [P55008-2]
56755 [P55008-3]
TopDownProteomicsiP55008-1 [P55008-1]

PTM databases

iPTMnetiP55008
PhosphoSitePlusiP55008

Expressioni

Tissue specificityi

Detected in T-lymphocytes and peripheral blood mononuclear cells.1 Publication

Gene expression databases

BgeeiENSG00000204472 Expressed in 214 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_AIF1
ExpressionAtlasiP55008 baseline and differential
GenevisibleiP55008 HS

Organism-specific databases

HPAiHPA049234
HPA062949

Interactioni

Subunit structurei

Homodimer (Potential). Monomer. Interacts with LCP1.Curated2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106702, 1 interactor
IntActiP55008, 2 interactors
MINTiP55008
STRINGi9606.ENSP00000365227

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP55008
SMRiP55008
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP55008

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 80EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 115EF-hand 2; degeneratePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410INJA Eukaryota
ENOG411206J LUCA
GeneTreeiENSGT00390000013846
HOGENOMiHOG000231928
HOVERGENiHBG004002
InParanoidiP55008
KOiK18617
PhylomeDBiP55008
TreeFamiTF320736

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P55008-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQTRDLQGG KAFGLLKAQQ EERLDEINKQ FLDDPKYSSD EDLPSKLEGF
60 70 80 90 100
KEKYMEFDLN GNGDIDIMSL KRMLEKLGVP KTHLELKKLI GEVSSGSGET
110 120 130 140
FSYPDFLRMM LGKRSAILKM ILMYEEKARE KEKPTGPPAK KAISELP
Length:147
Mass (Da):16,703
Last modified:October 1, 1996 - v1
Checksum:i0104CCF092074E8F
GO
Isoform 2 (identifier: P55008-2) [UniParc]FASTAAdd to basket
Also known as: G1

The sequence of this isoform differs from the canonical sequence as follows:
     1-54: Missing.

Show »
Length:93
Mass (Da):10,455
Checksum:i4365FCB38F2B7329
GO
Isoform 3 (identifier: P55008-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MSQTRDLQGG...EFDLNGNGDI → MEFDLNGNGD...PSPHPHPLPP
     121-147: Missing.

Show »
Length:132
Mass (Da):14,617
Checksum:iFC69F38ECC92A697
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5STX8Q5STX8_HUMAN
Allograft inflammatory factor 1
AIF1
161Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti33D → H in AAA92457 (PubMed:8081366).Curated1
Sequence conflicti131 – 147KEKPT…ISELP → RKTNTPPSQESPI in AAA92457 (PubMed:8081366).CuratedAdd BLAST17

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04866514G → R2 PublicationsCorresponds to variant dbSNP:rs2736182Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0438371 – 65MSQTR…GNGDI → MEFDLNGNGDIGEKRVICGG RVVCRPKKTEVSPTCSIPHD LGGGPPTTVGGRRMGMRKWE RRERVSPPSPHPHPLPP in isoform 3. 2 PublicationsAdd BLAST65
Alternative sequenceiVSP_0438381 – 54Missing in isoform 2. 3 PublicationsAdd BLAST54
Alternative sequenceiVSP_043839121 – 147Missing in isoform 3. 2 PublicationsAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49392 mRNA Translation: AAA92457.1
U95213 mRNA Translation: AAC24422.1
U19713 mRNA Translation: AAB05003.1
Y14768 Genomic DNA Translation: CAA75060.1
Y14768 Genomic DNA Translation: CAA75061.1
Y14768 Genomic DNA Translation: CAA75062.1
D86438 mRNA Translation: BAA13088.1
AF299327 mRNA Translation: AAG39110.1
EF070982 mRNA Translation: ABK35646.1
AK290771 mRNA Translation: BAF83460.1
CR542153 mRNA Translation: CAG46950.1
AF129756 Genomic DNA Translation: AAD18087.1
BA000025 Genomic DNA Translation: BAB63392.1
AL662801 Genomic DNA No translation available.
AL662847 Genomic DNA No translation available.
AL805934 Genomic DNA No translation available.
BX248305 Genomic DNA No translation available.
CR753892 Genomic DNA No translation available.
CR759761 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03442.1
CH471081 Genomic DNA Translation: EAX03446.1
BC009474 mRNA Translation: AAH09474.1
CCDSiCCDS34398.1 [P55008-2]
CCDS4706.1 [P55008-1]
PIRiJC5246
RefSeqiNP_001305899.1, NM_001318970.1 [P55008-2]
NP_001614.3, NM_001623.4 [P55008-1]
NP_116573.1, NM_032955.2 [P55008-2]
XP_016865821.1, XM_017010332.1 [P55008-3]
UniGeneiHs.76364

Genome annotation databases

EnsembliENST00000376049; ENSP00000365217; ENSG00000204472 [P55008-2]
ENST00000376059; ENSP00000365227; ENSG00000204472 [P55008-1]
ENST00000383473; ENSP00000372965; ENSG00000206428 [P55008-2]
ENST00000383474; ENSP00000372966; ENSG00000206428 [P55008-1]
ENST00000413349; ENSP00000416061; ENSG00000235985 [P55008-1]
ENST00000414149; ENSP00000391720; ENSG00000237727 [P55008-1]
ENST00000415830; ENSP00000413709; ENSG00000235985 [P55008-2]
ENST00000416422; ENSP00000399901; ENSG00000234836 [P55008-2]
ENST00000419376; ENSP00000401433; ENSG00000237727 [P55008-2]
ENST00000424944; ENSP00000399621; ENSG00000235588 [P55008-2]
ENST00000425748; ENSP00000398013; ENSG00000234836 [P55008-1]
ENST00000440907; ENSP00000397842; ENSG00000235588 [P55008-1]
GeneIDi199
KEGGihsa:199
UCSCiuc003nva.4 human [P55008-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49392 mRNA Translation: AAA92457.1
U95213 mRNA Translation: AAC24422.1
U19713 mRNA Translation: AAB05003.1
Y14768 Genomic DNA Translation: CAA75060.1
Y14768 Genomic DNA Translation: CAA75061.1
Y14768 Genomic DNA Translation: CAA75062.1
D86438 mRNA Translation: BAA13088.1
AF299327 mRNA Translation: AAG39110.1
EF070982 mRNA Translation: ABK35646.1
AK290771 mRNA Translation: BAF83460.1
CR542153 mRNA Translation: CAG46950.1
AF129756 Genomic DNA Translation: AAD18087.1
BA000025 Genomic DNA Translation: BAB63392.1
AL662801 Genomic DNA No translation available.
AL662847 Genomic DNA No translation available.
AL805934 Genomic DNA No translation available.
BX248305 Genomic DNA No translation available.
CR753892 Genomic DNA No translation available.
CR759761 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03442.1
CH471081 Genomic DNA Translation: EAX03446.1
BC009474 mRNA Translation: AAH09474.1
CCDSiCCDS34398.1 [P55008-2]
CCDS4706.1 [P55008-1]
PIRiJC5246
RefSeqiNP_001305899.1, NM_001318970.1 [P55008-2]
NP_001614.3, NM_001623.4 [P55008-1]
NP_116573.1, NM_032955.2 [P55008-2]
XP_016865821.1, XM_017010332.1 [P55008-3]
UniGeneiHs.76364

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2D58X-ray1.90A17-123[»]
2G2BNMR-A2-147[»]
ProteinModelPortaliP55008
SMRiP55008
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106702, 1 interactor
IntActiP55008, 2 interactors
MINTiP55008
STRINGi9606.ENSP00000365227

PTM databases

iPTMnetiP55008
PhosphoSitePlusiP55008

Polymorphism and mutation databases

BioMutaiAIF1
DMDMi1703217

Proteomic databases

EPDiP55008
PaxDbiP55008
PeptideAtlasiP55008
PRIDEiP55008
ProteomicsDBi56753
56754 [P55008-2]
56755 [P55008-3]
TopDownProteomicsiP55008-1 [P55008-1]

Protocols and materials databases

DNASUi199
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376049; ENSP00000365217; ENSG00000204472 [P55008-2]
ENST00000376059; ENSP00000365227; ENSG00000204472 [P55008-1]
ENST00000383473; ENSP00000372965; ENSG00000206428 [P55008-2]
ENST00000383474; ENSP00000372966; ENSG00000206428 [P55008-1]
ENST00000413349; ENSP00000416061; ENSG00000235985 [P55008-1]
ENST00000414149; ENSP00000391720; ENSG00000237727 [P55008-1]
ENST00000415830; ENSP00000413709; ENSG00000235985 [P55008-2]
ENST00000416422; ENSP00000399901; ENSG00000234836 [P55008-2]
ENST00000419376; ENSP00000401433; ENSG00000237727 [P55008-2]
ENST00000424944; ENSP00000399621; ENSG00000235588 [P55008-2]
ENST00000425748; ENSP00000398013; ENSG00000234836 [P55008-1]
ENST00000440907; ENSP00000397842; ENSG00000235588 [P55008-1]
GeneIDi199
KEGGihsa:199
UCSCiuc003nva.4 human [P55008-1]

Organism-specific databases

CTDi199
DisGeNETi199
EuPathDBiHostDB:ENSG00000204472.12
GeneCardsiAIF1
HGNCiHGNC:352 AIF1
HPAiHPA049234
HPA062949
MIMi601833 gene
neXtProtiNX_P55008
OpenTargetsiENSG00000204472
PharmGKBiPA24646
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INJA Eukaryota
ENOG411206J LUCA
GeneTreeiENSGT00390000013846
HOGENOMiHOG000231928
HOVERGENiHBG004002
InParanoidiP55008
KOiK18617
PhylomeDBiP55008
TreeFamiTF320736

Miscellaneous databases

ChiTaRSiAIF1 human
EvolutionaryTraceiP55008
GeneWikiiAIF1
GenomeRNAii199
PROiPR:P55008
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000204472 Expressed in 214 organ(s), highest expression level in metanephric glomerulus
CleanExiHS_AIF1
ExpressionAtlasiP55008 baseline and differential
GenevisibleiP55008 HS

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAIF1_HUMAN
AccessioniPrimary (citable) accession number: P55008
Secondary accession number(s): A8K406
, O43904, Q9UIV4, Q9UKS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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