UniProtKB - P54999 (RUXF_YEAST)
Small nuclear ribonucleoprotein F
SMX3
Functioni
Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (By similarity).
By similarityMiscellaneous
GO - Molecular functioni
- poly(U) RNA binding Source: SGD
- RNA binding Source: GO_Central
- splicing factor binding Source: SGD
GO - Biological processi
- 7-methylguanosine cap hypermethylation Source: ComplexPortal
- mRNA 5'-splice site recognition Source: ComplexPortal
- mRNA splicing, via spliceosome Source: ComplexPortal
- spliceosomal complex assembly Source: ComplexPortal
- spliceosomal snRNP assembly Source: ComplexPortal
- U2-type prespliceosome assembly Source: ComplexPortal
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Names & Taxonomyi
Protein namesi | Recommended name: Small nuclear ribonucleoprotein FShort name: snRNP-F Alternative name(s): Sm protein F Short name: Sm-F Short name: SmF |
Gene namesi | Name:SMX3 Ordered Locus Names:YPR182W ORF Names:P9705.4 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006386, SMX3 |
VEuPathDBi | FungiDB:YPR182W |
Subcellular locationi
Nucleus
- catalytic step 2 spliceosome Source: GO_Central
- commitment complex Source: ComplexPortal
- nucleus Source: ComplexPortal
- spliceosomal complex Source: ComplexPortal
- U1 snRNP Source: SGD
- U2 snRNP Source: SGD
- U4 snRNP Source: ComplexPortal
- U4/U6 snRNP Source: ComplexPortal
- U4/U6 x U5 tri-snRNP complex Source: SGD
- U5 snRNP Source: SGD
Other locations
- cytoplasm Source: ComplexPortal
Keywords - Cellular componenti
Cytoplasm, Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000125544 | 1 – 86 | Small nuclear ribonucleoprotein FAdd BLAST | 86 |
Proteomic databases
MaxQBi | P54999 |
PaxDbi | P54999 |
PRIDEi | P54999 |
Interactioni
Subunit structurei
Component of the Sm core complex, present in spliceosomal snRNP U1, U2, U4/U6 and U5. The core complex contains SMB1, SMD1, SMD2, SMD3, SME1, SMX3 and SMX2 (Sm proteins B, D1, D2, D3, E, F and G, respectively), and is probably a heptameric ring structure. SMX3 specifically interacts with SME1. Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRP3, PRP4, PRP6, PRP8, PRP18, PRP31, PRP38, SNU13, SNU23, SNU66, SNU114, SPP381, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, LSM2, LSM3, LSM4, LSM5, LSM6, LSM7, LSM8, BRR2 and DIB1.
4 PublicationsBinary interactionsi
P54999
With | #Exp. | IntAct |
---|---|---|
SME1 [Q12330] | 6 | EBI-770,EBI-16359 |
GO - Molecular functioni
- splicing factor binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 36354, 571 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-23, U1 small nuclear ribonucleoprotein complex CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-26, U2 small nuclear ribonucleoprotein complex CPX-29, U5 small nuclear ribonucleoprotein complex CPX-30, U5 small nuclear ribonucleoprotein complex, AAR2 variant CPX-31, U4 small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex CPX-43, Sm complex |
DIPi | DIP-1634N |
IntActi | P54999, 45 interactors |
MINTi | P54999 |
STRINGi | 4932.YPR182W |
Miscellaneous databases
RNActi | P54999, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P54999 |
SMRi | P54999 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54999 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3482, Eukaryota |
GeneTreei | ENSGT00940000154818 |
HOGENOMi | CLU_076902_12_3_1 |
InParanoidi | P54999 |
OMAi | VKLKWGH |
Family and domain databases
CDDi | cd01722, Sm_F, 1 hit |
InterProi | View protein in InterPro IPR016487, Lsm6/sSmF IPR001163, LSM_dom_euk/arc IPR010920, LSM_dom_sf IPR034100, Sm_F |
PANTHERi | PTHR11021, PTHR11021, 1 hit |
Pfami | View protein in Pfam PF01423, LSM, 1 hit |
SMARTi | View protein in SMART SM00651, Sm, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN
60 70 80
LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82778 Genomic DNA Translation: CAA58022.1 U25842 Genomic DNA Translation: AAB68115.1 AY558049 Genomic DNA Translation: AAS56375.1 BK006949 Genomic DNA Translation: DAA11598.1 |
PIRi | S55055 |
RefSeqi | NP_015508.1, NM_001184279.1 |
Genome annotation databases
EnsemblFungii | YPR182W_mRNA; YPR182W; YPR182W |
GeneIDi | 856312 |
KEGGi | sce:YPR182W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82778 Genomic DNA Translation: CAA58022.1 U25842 Genomic DNA Translation: AAB68115.1 AY558049 Genomic DNA Translation: AAS56375.1 BK006949 Genomic DNA Translation: DAA11598.1 |
PIRi | S55055 |
RefSeqi | NP_015508.1, NM_001184279.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N9R | X-ray | 2.80 | A/B/C/D/E/F/G | 1-86 | [»] | |
1N9S | X-ray | 3.50 | A/B/C/D/E/F/G/H/I/J/K/L/M/N | 1-86 | [»] | |
3JCM | electron microscopy | 3.80 | W/Z | 1-86 | [»] | |
5GAM | electron microscopy | 3.70 | f | 1-86 | [»] | |
5GAN | electron microscopy | 3.60 | f/q | 1-86 | [»] | |
5GAO | electron microscopy | 3.60 | q | 1-86 | [»] | |
5GM6 | electron microscopy | 3.50 | h | 1-86 | [»] | |
5GMK | electron microscopy | 3.40 | h/w | 1-86 | [»] | |
5LJ3 | electron microscopy | 3.80 | f/q | 1-86 | [»] | |
5LJ5 | electron microscopy | 3.80 | f/q | 1-86 | [»] | |
5LQW | electron microscopy | 5.80 | f | 1-86 | [»] | |
5MPS | electron microscopy | 3.85 | f | 1-86 | [»] | |
5MQ0 | electron microscopy | 4.17 | f/q | 1-86 | [»] | |
5NRL | electron microscopy | 7.20 | f/q/x | 1-86 | [»] | |
5WSG | electron microscopy | 4.00 | H/h | 1-86 | [»] | |
5Y88 | electron microscopy | 3.70 | c/j | 1-86 | [»] | |
5YLZ | electron microscopy | 3.60 | c/j | 1-86 | [»] | |
5ZWM | electron microscopy | 3.40 | U/f/j | 1-86 | [»] | |
5ZWN | electron microscopy | 3.30 | f | 1-86 | [»] | |
5ZWO | electron microscopy | 3.90 | U/f/j | 1-86 | [»] | |
6BK8 | electron microscopy | 3.30 | b/m | 1-86 | [»] | |
6EXN | electron microscopy | 3.70 | f/q | 1-86 | [»] | |
6G90 | electron microscopy | 4.00 | f/x | 1-86 | [»] | |
6J6G | electron microscopy | 3.20 | h/w | 1-86 | [»] | |
6J6H | electron microscopy | 3.60 | h/w | 1-86 | [»] | |
6J6N | electron microscopy | 3.86 | h/w | 1-86 | [»] | |
6J6Q | electron microscopy | 3.70 | h/w | 1-86 | [»] | |
6N7P | electron microscopy | 3.60 | P | 1-86 | [»] | |
6N7R | electron microscopy | 3.20 | P | 1-86 | [»] | |
6N7X | electron microscopy | 3.60 | P | 1-86 | [»] | |
7B9V | electron microscopy | 2.80 | f/q | 1-86 | [»] | |
7OQB | electron microscopy | 9.00 | x | 1-86 | [»] | |
7OQC | electron microscopy | 4.10 | f | 1-86 | [»] | |
7OQE | electron microscopy | 5.90 | f/x | 1-86 | [»] | |
AlphaFoldDBi | P54999 | |||||
SMRi | P54999 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 36354, 571 interactors |
ComplexPortali | CPX-1651, PRP19-associated complex CPX-23, U1 small nuclear ribonucleoprotein complex CPX-25, U4/U6.U5 tri-small nuclear ribonucleoprotein complex CPX-26, U2 small nuclear ribonucleoprotein complex CPX-29, U5 small nuclear ribonucleoprotein complex CPX-30, U5 small nuclear ribonucleoprotein complex, AAR2 variant CPX-31, U4 small nuclear ribonucleoprotein complex CPX-32, U4/U6 small nuclear ribonucleoprotein complex CPX-43, Sm complex |
DIPi | DIP-1634N |
IntActi | P54999, 45 interactors |
MINTi | P54999 |
STRINGi | 4932.YPR182W |
Proteomic databases
MaxQBi | P54999 |
PaxDbi | P54999 |
PRIDEi | P54999 |
Genome annotation databases
EnsemblFungii | YPR182W_mRNA; YPR182W; YPR182W |
GeneIDi | 856312 |
KEGGi | sce:YPR182W |
Organism-specific databases
SGDi | S000006386, SMX3 |
VEuPathDBi | FungiDB:YPR182W |
Phylogenomic databases
eggNOGi | KOG3482, Eukaryota |
GeneTreei | ENSGT00940000154818 |
HOGENOMi | CLU_076902_12_3_1 |
InParanoidi | P54999 |
OMAi | VKLKWGH |
Miscellaneous databases
EvolutionaryTracei | P54999 |
PROi | PR:P54999 |
RNActi | P54999, protein |
Family and domain databases
CDDi | cd01722, Sm_F, 1 hit |
InterProi | View protein in InterPro IPR016487, Lsm6/sSmF IPR001163, LSM_dom_euk/arc IPR010920, LSM_dom_sf IPR034100, Sm_F |
PANTHERi | PTHR11021, PTHR11021, 1 hit |
Pfami | View protein in Pfam PF01423, LSM, 1 hit |
SMARTi | View protein in SMART SM00651, Sm, 1 hit |
SUPFAMi | SSF50182, SSF50182, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RUXF_YEAST | |
Accessioni | P54999Primary (citable) accession number: P54999 Secondary accession number(s): D6W4I2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 194 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families