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Protein

IAA-amino acid hydrolase ILR1-like 2

Gene

ILL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala, IAA-Leu, IAA-Met, IAA-Phe, IAA-Ser, IAA-Thr, IAA-Tyr and IAA-Val (PubMed:11923288). Is the most efficient enzyme of the ILL family for IAA-Ala (PubMed:11923288). Not important for IAA-Leu hydrolysis in roots (PubMed:15155875). May act with ILR1 to provide free IAA to germinating seedlings (PubMed:15155875).2 Publications

Cofactori

Mn2+1 PublicationNote: The Mn2+ ion enhances activity.1 Publication

Kineticsi

kcat is 2.1 sec(-1) with IAA-Ala as substrate. kcat is 0.27 sec(-1) with IAA-Leu as substrate.1 Publication
  1. KM=52 µM for IAA-Ala1 Publication
  2. KM=126 µM for IAA-Leu1 Publication
  1. Vmax=1700 nmol/min/mg enzyme with IAA-Ala as substrate1 Publication
  2. Vmax=220 nmol/min/mg enzyme with IAA-Leu as substrate1 Publication

pH dependencei

Optimum pH is 8.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi137Manganese 1By similarity1 Publication1
Metal bindingi137Manganese 2By similarity1 Publication1
Metal bindingi139Manganese 2By similarity1 Publication1
Metal bindingi173Manganese 1By similarity1 Publication1
Metal bindingi197Manganese 2By similarity1 Publication1
Metal bindingi397Manganese 1By similarity1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4142

Protein family/group databases

MEROPSiM20.014

Names & Taxonomyi

Protein namesi
Recommended name:
IAA-amino acid hydrolase ILR1-like 21 Publication (EC:3.5.1.-Curated)
Gene namesi
Name:ILL21 Publication
Ordered Locus Names:At5g56660Imported
ORF Names:MIK19.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G56660
TAIRilocus:2164976 AT5G56660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

No effect on the sensitivity to the inhibition of root elongation caused by IAA-Leu or IAA-Ala.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000119022 – 439IAA-amino acid hydrolase ILR1-like 2Add BLAST418

Proteomic databases

PaxDbiP54970
PRIDEiP54970

Expressioni

Tissue specificityi

Expressed in leaves, stems, siliques, seeds and flowers. Detected in the distal tips of cotyledons and seedling leaves, hydathodes of leaves from mature plants, pollen, ovules and developing seeds.1 Publication

Gene expression databases

ExpressionAtlasiP54970 baseline and differential
GenevisibleiP54970 AT

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G56660.1

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP54970
SMRiP54970
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54970

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi436 – 439Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the peptidase M20 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIRP Eukaryota
COG1473 LUCA
HOGENOMiHOG000241403
InParanoidiP54970
KOiK14664
OMAiVMIHQHA
OrthoDBiEOG09360A3R
PhylomeDBiP54970

Family and domain databases

InterProiView protein in InterPro
IPR017439 Amidohydrolase
IPR036264 Bact_exopeptidase_dim_dom
IPR002933 Peptidase_M20
IPR011650 Peptidase_M20_dimer
PfamiView protein in Pfam
PF07687 M20_dimer, 1 hit
PF01546 Peptidase_M20, 1 hit
PIRSFiPIRSF005962 Pept_M20D_amidohydro, 1 hit
SUPFAMiSSF55031 SSF55031, 1 hit
TIGRFAMsiTIGR01891 amidohydrolases, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P54970-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALNKLLSLT FQLLLFLLSV SSESPWIAED TSQIQTKLLE FAKSPEVFDW
60 70 80 90 100
MVKIRRKIHE NPELGYEELE TSKLIRSELE LIGIKYRYPV AITGVIGYIG
110 120 130 140 150
TGEPPFVALR ADMDALPIQE GVEWEHKSKI AGKMHACGHD GHVTMLLGAA
160 170 180 190 200
KILHEHRHHL QGTVVLIFQP AEEGLSGAKK MREEGALKNV EAIFGIHLSA
210 220 230 240 250
RIPFGKAASR AGSFLAGAGV FEAVITGKGG HAAIPQHTID PVVAASSIVL
260 270 280 290 300
SLQQLVSRET DPLDSKVVTV SKVNGGNAFN VIPDSITIGG TLRAFTGFTQ
310 320 330 340 350
LQQRVKEVIT KQAAVHRCNA SVNLTPNGRE PMPPTVNNKD LYKQFKKVVR
360 370 380 390 400
DLLGQEAFVE AAPVMGSEDF SYFAETIPGH FSLLGMQDET NGYASSHSPL
410 420 430
YRINEDVLPY GAAIHASMAV QYLKEKASKG SVSGFHEEL
Length:439
Mass (Da):47,856
Last modified:May 27, 2002 - v2
Checksum:iBFA5F35AF4C4F508
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131A → P in AAC49016 (PubMed:7792599).Curated1
Sequence conflicti236Q → H in AAC49016 (PubMed:7792599).Curated1
Sequence conflicti240D → G in AAL59907 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23796 mRNA Translation: AAC49016.1
AF047031 Genomic DNA Translation: AAC04866.1
AB013392 Genomic DNA Translation: BAB09884.1
CP002688 Genomic DNA Translation: AED96793.1
AY072084 mRNA Translation: AAL59907.1
RefSeqiNP_200477.1, NM_125049.3
UniGeneiAt.46738
At.66661
At.66838

Genome annotation databases

EnsemblPlantsiAT5G56660.1; AT5G56660.1; AT5G56660
GeneIDi835767
GrameneiAT5G56660.1; AT5G56660.1; AT5G56660
KEGGiath:AT5G56660

Similar proteinsi

Entry informationi

Entry nameiILL2_ARATH
AccessioniPrimary (citable) accession number: P54970
Secondary accession number(s): O49221
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: May 23, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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