ILL2

Functionⁱ

Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala, IAA-Leu, IAA-Met, IAA-Phe, IAA-Ser, IAA-Thr, IAA-Tyr and IAA-Val (PubMed:11923288). Is the most efficient enzyme of the ILL family for IAA-Ala (PubMed:11923288). Not important for IAA-Leu hydrolysis in roots (PubMed:15155875). May act with ILR1 to provide free IAA to germinating seedlings (PubMed:15155875).2 Publications

Cofactorⁱ

Mn²⁺1 PublicationNote: The Mn²⁺ ion enhances activity.1 Publication

Kineticsⁱ

kcat is 2.1 sec(-1) with IAA-Ala as substrate. kcat is 0.27 sec(-1) with IAA-Leu as substrate.1 Publication

pH dependenceⁱ

Optimum pH is 8.0.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	137	Manganese 1By similarity1 Publication	1
Metal bindingⁱ	137	Manganese 2By similarity1 Publication	1
Metal bindingⁱ	139	Manganese 2By similarity1 Publication	1
Metal bindingⁱ	173	Manganese 1By similarity1 Publication	1
Metal bindingⁱ	197	Manganese 2By similarity1 Publication	1
Metal bindingⁱ	397	Manganese 1By similarity1 Publication	1

GO - Molecular functionⁱ

IAA-Ala conjugate hydrolase activity
Source: TAIR
Inferred from direct assayⁱ
- 11923288
IAA-amino acid conjugate hydrolase activity
Source: TAIR
Inferred from direct assayⁱ
- 11923288
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

auxin metabolic process
Source: TAIR
Inferred from direct assayⁱ
- 11923288

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Ligand	Manganese, Metal-binding

Enzyme and pathway databases

BioCycⁱ	MetaCyc:MONOMER-4142

BioCycⁱ

MetaCyc:MONOMER-4142

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	M20.014

MEROPSⁱ

M20.014

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: IAA-amino acid hydrolase ILR1-like 21 Publication (EC:3.5.1.-Curated)
Gene namesⁱ	Name:ILL21 Publication Ordered Locus Names:At5g56660Imported ORF Names:MIK19.11Imported
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT5G56660
TAIRⁱ	locus:2164976 AT5G56660

Keywords - Cellular componentⁱ

Endoplasmic reticulum

Pathology & Biotechⁱ

Disruption phenotypeⁱ

No effect on the sensitivity to the inhibition of root elongation caused by IAA-Leu or IAA-Ala.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000001190	22 – 439	IAA-amino acid hydrolase ILR1-like 2Add BLAST		418

Proteomic databases

PaxDbⁱ	P54970
PRIDEⁱ	P54970

Expressionⁱ

Tissue specificityⁱ

Expressed in leaves, stems, siliques, seeds and flowers. Detected in the distal tips of cotyledons and seedling leaves, hydathodes of leaves from mature plants, pollen, ovules and developing seeds.1 Publication

Gene expression databases

ExpressionAtlasⁱ	P54970 baseline and differential
Genevisibleⁱ	P54970 AT

Interactionⁱ

Subunit structureⁱ

Monomer.1 Publication

Protein-protein interaction databases

STRINGⁱ	3702.AT5G56660.1

STRINGⁱ

3702.AT5G56660.1

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	38 – 43	Combined sources	6
Helixⁱ	45 – 60	Combined sources	16
Helixⁱ	69 – 82	Combined sources	14
Beta strandⁱ	86 – 90	Combined sources	5
Turnⁱ	91 – 93	Combined sources	3
Beta strandⁱ	94 – 104	Combined sources	11
Beta strandⁱ	106 – 112	Combined sources	7
Helixⁱ	139 – 155	Combined sources	17
Helixⁱ	157 – 159	Combined sources	3
Beta strandⁱ	161 – 169	Combined sources	9
Turnⁱ	172 – 175	Combined sources	4
Helixⁱ	177 – 183	Combined sources	7
Turnⁱ	184 – 189	Combined sources	6
Beta strandⁱ	190 – 203	Combined sources	14
Beta strandⁱ	207 – 209	Combined sources	3
Beta strandⁱ	211 – 215	Combined sources	5
Beta strandⁱ	217 – 227	Combined sources	11
Helixⁱ	240 – 253	Combined sources	14
Helixⁱ	262 – 264	Combined sources	3
Beta strandⁱ	266 – 274	Combined sources	9
Beta strandⁱ	285 – 296	Combined sources	12
Helixⁱ	298 – 315	Combined sources	18
Beta strandⁱ	318 – 325	Combined sources	8
Helixⁱ	326 – 328	Combined sources	3
Beta strandⁱ	335 – 337	Combined sources	3
Helixⁱ	339 – 353	Combined sources	15
Helixⁱ	355 – 357	Combined sources	3
Beta strandⁱ	358 – 360	Combined sources	3
Helixⁱ	370 – 374	Combined sources	5
Turnⁱ	375 – 378	Combined sources	4
Beta strandⁱ	379 – 387	Combined sources	9
Beta strandⁱ	396 – 398	Combined sources	3
Helixⁱ	405 – 407	Combined sources	3
Helixⁱ	408 – 427	Combined sources	20

Show more details

3D structure databases

ProteinModelPortalⁱ	P54970
SMRⁱ	P54970
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P54970

EvolutionaryTraceⁱ

P54970

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	436 – 439	Prevents secretion from ERPROSITE-ProRule annotation		4

Sequence similaritiesⁱ

Belongs to the peptidase M20 family.Curated

Keywords - Domainⁱ

Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IIRP Eukaryota COG1473 LUCA
HOGENOMⁱ	HOG000241403
InParanoidⁱ	P54970
KEGG Orthology (KO) More...KOⁱ	K14664
OMAⁱ	VMIHQHA
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360A3R
PhylomeDBⁱ	P54970

Family and domain databases

InterProⁱ	View protein in InterPro IPR017439 Amidohydrolase IPR036264 Bact_exopeptidase_dim_dom IPR002933 Peptidase_M20 IPR011650 Peptidase_M20_dimer
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07687 M20_dimer, 1 hit PF01546 Peptidase_M20, 1 hit
PIRSFⁱ	PIRSF005962 Pept_M20D_amidohydro, 1 hit
SUPFAMⁱ	SSF55031 SSF55031, 1 hit
TIGRFAMsⁱ	TIGR01891 amidohydrolases, 1 hit
PROSITEⁱ	View protein in PROSITE PS00014 ER_TARGET, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P54970-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MALNKLLSLT FQLLLFLLSV SSESPWIAED TSQIQTKLLE FAKSPEVFDW 
        60         70         80         90        100
MVKIRRKIHE NPELGYEELE TSKLIRSELE LIGIKYRYPV AITGVIGYIG 
       110        120        130        140        150
TGEPPFVALR ADMDALPIQE GVEWEHKSKI AGKMHACGHD GHVTMLLGAA 
       160        170        180        190        200
KILHEHRHHL QGTVVLIFQP AEEGLSGAKK MREEGALKNV EAIFGIHLSA 
       210        220        230        240        250
RIPFGKAASR AGSFLAGAGV FEAVITGKGG HAAIPQHTID PVVAASSIVL 
       260        270        280        290        300
SLQQLVSRET DPLDSKVVTV SKVNGGNAFN VIPDSITIGG TLRAFTGFTQ 
       310        320        330        340        350
LQQRVKEVIT KQAAVHRCNA SVNLTPNGRE PMPPTVNNKD LYKQFKKVVR 
       360        370        380        390        400
DLLGQEAFVE AAPVMGSEDF SYFAETIPGH FSLLGMQDET NGYASSHSPL 
       410        420        430 
YRINEDVLPY GAAIHASMAV QYLKEKASKG SVSGFHEEL

Length:439

Mass (Da):47,856

Last modified:May 27, 2002 - v2

Checksum:ⁱBFA5F35AF4C4F508

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	131	A → P in AAC49016 (PubMed:7792599).Curated	1
Sequence conflictⁱ	236	Q → H in AAC49016 (PubMed:7792599).Curated	1
Sequence conflictⁱ	240	D → G in AAL59907 (PubMed:14593172).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U23796 mRNA Translation: AAC49016.1 AF047031 Genomic DNA Translation: AAC04866.1 AB013392 Genomic DNA Translation: BAB09884.1 CP002688 Genomic DNA Translation: AED96793.1 AY072084 mRNA Translation: AAL59907.1
NCBI Reference Sequences More...RefSeqⁱ	NP_200477.1, NM_125049.3
UniGeneⁱ	At.46738 At.66661 At.66838

Genome annotation databases

EnsemblPlantsⁱ	AT5G56660.1; AT5G56660.1; AT5G56660
GeneIDⁱ	835767
Grameneⁱ	AT5G56660.1; AT5G56660.1; AT5G56660
KEGGⁱ	ath:AT5G56660

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P54970	At5g56660		ARATH		439	UniRef100_P54970
UPI000044F678		ARATH		418

Protein	Similar proteins		Species	Score	Length	Source
P54970	IAA-amino acid hydrolase ILR1-like 1		ARATH		438	UniRef90_P54970
Uncharacterized protein		ARALL		436
At5g56660		ARATH		439
ILL2		ARATH		439
At5g56650		ARATH		438
+3

Protein	Similar proteins		Species	Score	Length	Source
P54970	IAA-amino acid hydrolase ILR1-like 4		ARATH		440	UniRef50_O04373
Uncharacterized protein		Capsella rubella		440
IAA-alanine resistant 3		ARALL		440
Auxin amidohydrolase		WHEAT		437
Uncharacterized protein		BRADI		444
+377

Entry informationⁱ

Entry nameⁱ	ILL2_ARATH
Accessionⁱ	P54970Primary (citable) accession number: P54970 Secondary accession number(s): O49221
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	May 27, 2002
	Last modified:	May 23, 2018
	This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P54970 (ILL2_ARATH)

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IAA-amino acid hydrolase ILR1-like 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

pH dependencei

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ