UniProtKB - P54970 (ILL2_ARATH)
Protein
IAA-amino acid hydrolase ILR1-like 2
Gene
ILL2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Hydrolyzes certain amino acid conjugates of the plant growth regulator indole-3-acetic acid (IAA), including IAA-Ala, IAA-Leu, IAA-Met, IAA-Phe, IAA-Ser, IAA-Thr, IAA-Tyr and IAA-Val (PubMed:11923288). Is the most efficient enzyme of the ILL family for IAA-Ala (PubMed:11923288). Not important for IAA-Leu hydrolysis in roots (PubMed:15155875). May act with ILR1 to provide free IAA to germinating seedlings (PubMed:15155875).2 Publications
Cofactori
Mn2+1 PublicationNote: The Mn2+ ion enhances activity.1 Publication
Kineticsi
kcat is 2.1 sec(-1) with IAA-Ala as substrate. kcat is 0.27 sec(-1) with IAA-Leu as substrate.1 Publication
- KM=52 µM for IAA-Ala1 Publication
- KM=126 µM for IAA-Leu1 Publication
- Vmax=1700 nmol/min/mg enzyme with IAA-Ala as substrate1 Publication
- Vmax=220 nmol/min/mg enzyme with IAA-Leu as substrate1 Publication
pH dependencei
Optimum pH is 8.0.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 137 | Manganese 1By similarity1 Publication | 1 | |
Metal bindingi | 137 | Manganese 2By similarity1 Publication | 1 | |
Metal bindingi | 139 | Manganese 2By similarity1 Publication | 1 | |
Metal bindingi | 173 | Manganese 1By similarity1 Publication | 1 | |
Metal bindingi | 197 | Manganese 2By similarity1 Publication | 1 | |
Metal bindingi | 397 | Manganese 1By similarity1 Publication | 1 |
GO - Molecular functioni
- hydrolase activity Source: GO_Central
- IAA-Ala conjugate hydrolase activity Source: TAIR
- IAA-amino acid conjugate hydrolase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- auxin metabolic process Source: TAIR
Keywordsi
Molecular function | Hydrolase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-4142 MetaCyc:MONOMER-4142 |
Protein family/group databases
MEROPSi | M20.014 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ILL21 Publication Ordered Locus Names:At5g56660Imported ORF Names:MIK19.11Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G56660 |
TAIRi | locus:2164976, AT5G56660 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Disruption phenotypei
No effect on the sensitivity to the inhibition of root elongation caused by IAA-Leu or IAA-Ala.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000001190 | 22 – 439 | IAA-amino acid hydrolase ILR1-like 2Add BLAST | 418 |
Proteomic databases
PaxDbi | P54970 |
PRIDEi | P54970 |
ProteomicsDBi | 228838 |
Expressioni
Tissue specificityi
Expressed in leaves, stems, siliques, seeds and flowers. Detected in the distal tips of cotyledons and seedling leaves, hydathodes of leaves from mature plants, pollen, ovules and developing seeds.1 Publication
Gene expression databases
ExpressionAtlasi | P54970, baseline and differential |
Genevisiblei | P54970, AT |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 3702.AT5G56660.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P54970 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54970 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 436 – 439 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the peptidase M20 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QQEM, Eukaryota |
HOGENOMi | CLU_023257_0_0_1 |
InParanoidi | P54970 |
OMAi | ALHMCPW |
OrthoDBi | 1022341at2759 |
PhylomeDBi | P54970 |
Family and domain databases
InterProi | View protein in InterPro IPR017439, Amidohydrolase IPR036264, Bact_exopeptidase_dim_dom IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF005962, Pept_M20D_amidohydro, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
TIGRFAMsi | TIGR01891, amidohydrolases, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P54970-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALNKLLSLT FQLLLFLLSV SSESPWIAED TSQIQTKLLE FAKSPEVFDW
60 70 80 90 100
MVKIRRKIHE NPELGYEELE TSKLIRSELE LIGIKYRYPV AITGVIGYIG
110 120 130 140 150
TGEPPFVALR ADMDALPIQE GVEWEHKSKI AGKMHACGHD GHVTMLLGAA
160 170 180 190 200
KILHEHRHHL QGTVVLIFQP AEEGLSGAKK MREEGALKNV EAIFGIHLSA
210 220 230 240 250
RIPFGKAASR AGSFLAGAGV FEAVITGKGG HAAIPQHTID PVVAASSIVL
260 270 280 290 300
SLQQLVSRET DPLDSKVVTV SKVNGGNAFN VIPDSITIGG TLRAFTGFTQ
310 320 330 340 350
LQQRVKEVIT KQAAVHRCNA SVNLTPNGRE PMPPTVNNKD LYKQFKKVVR
360 370 380 390 400
DLLGQEAFVE AAPVMGSEDF SYFAETIPGH FSLLGMQDET NGYASSHSPL
410 420 430
YRINEDVLPY GAAIHASMAV QYLKEKASKG SVSGFHEEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 131 | A → P in AAC49016 (PubMed:7792599).Curated | 1 | |
Sequence conflicti | 236 | Q → H in AAC49016 (PubMed:7792599).Curated | 1 | |
Sequence conflicti | 240 | D → G in AAL59907 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23796 mRNA Translation: AAC49016.1 AF047031 Genomic DNA Translation: AAC04866.1 AB013392 Genomic DNA Translation: BAB09884.1 CP002688 Genomic DNA Translation: AED96793.1 AY072084 mRNA Translation: AAL59907.1 |
RefSeqi | NP_200477.1, NM_125049.3 |
Genome annotation databases
EnsemblPlantsi | AT5G56660.1; AT5G56660.1; AT5G56660 |
GeneIDi | 835767 |
Gramenei | AT5G56660.1; AT5G56660.1; AT5G56660 |
KEGGi | ath:AT5G56660 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U23796 mRNA Translation: AAC49016.1 AF047031 Genomic DNA Translation: AAC04866.1 AB013392 Genomic DNA Translation: BAB09884.1 CP002688 Genomic DNA Translation: AED96793.1 AY072084 mRNA Translation: AAL59907.1 |
RefSeqi | NP_200477.1, NM_125049.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1XMB | X-ray | 2.00 | A | 22-439 | [»] | |
2Q43 | X-ray | 2.00 | A | 22-439 | [»] | |
SMRi | P54970 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G56660.1 |
Protein family/group databases
MEROPSi | M20.014 |
Proteomic databases
PaxDbi | P54970 |
PRIDEi | P54970 |
ProteomicsDBi | 228838 |
Genome annotation databases
EnsemblPlantsi | AT5G56660.1; AT5G56660.1; AT5G56660 |
GeneIDi | 835767 |
Gramenei | AT5G56660.1; AT5G56660.1; AT5G56660 |
KEGGi | ath:AT5G56660 |
Organism-specific databases
Araporti | AT5G56660 |
TAIRi | locus:2164976, AT5G56660 |
Phylogenomic databases
eggNOGi | ENOG502QQEM, Eukaryota |
HOGENOMi | CLU_023257_0_0_1 |
InParanoidi | P54970 |
OMAi | ALHMCPW |
OrthoDBi | 1022341at2759 |
PhylomeDBi | P54970 |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-4142 MetaCyc:MONOMER-4142 |
Miscellaneous databases
EvolutionaryTracei | P54970 |
PROi | PR:P54970 |
Gene expression databases
ExpressionAtlasi | P54970, baseline and differential |
Genevisiblei | P54970, AT |
Family and domain databases
InterProi | View protein in InterPro IPR017439, Amidohydrolase IPR036264, Bact_exopeptidase_dim_dom IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
PIRSFi | PIRSF005962, Pept_M20D_amidohydro, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
TIGRFAMsi | TIGR01891, amidohydrolases, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ILL2_ARATH | |
Accessioni | P54970Primary (citable) accession number: P54970 Secondary accession number(s): O49221 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 27, 2002 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries