UniProtKB - P54921 (SNAA_RAT)
Protein
Alpha-soluble NSF attachment protein
Gene
Napa
Organism
Rattus norvegicus (Rat)
Status
Functioni
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Together with GNA12 promotes CDH5 localization to plasma membrane.By similarity
GO - Molecular functioni
- protein-containing complex binding Source: UniProtKB
- SNARE binding Source: ParkinsonsUK-UCL
- soluble NSF attachment protein activity Source: GO_Central
- syntaxin binding Source: UniProtKB
GO - Biological processi
- apical protein localization Source: RGD
- brain development Source: RGD
- intracellular protein transport Source: RGD
- membrane fusion Source: RGD
- neuron differentiation Source: RGD
- positive regulation of ATPase activity Source: UniProtKB
- protein-containing complex disassembly Source: UniProtKB
- regulation of synaptic vesicle priming Source: RGD
- SNARE complex disassembly Source: RGD
- synaptic transmission, glutamatergic Source: RGD
- synaptic vesicle priming Source: RGD
Keywordsi
Biological process | ER-Golgi transport, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-432722, Golgi Associated Vesicle Biogenesis R-RNO-6807878, COPI-mediated anterograde transport R-RNO-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-RNO-6811438, Intra-Golgi traffic R-RNO-6811440, Retrograde transport at the Trans-Golgi-Network |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-soluble NSF attachment proteinShort name: SNAP-alpha Alternative name(s): N-ethylmaleimide-sensitive factor attachment protein alpha |
Gene namesi | Name:Napa Synonyms:Snap, Snapa |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620855, Napa |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- glutamatergic synapse Source: RGD
- SNARE complex Source: GO_Central
- synaptobrevin 2-SNAP-25-syntaxin-1a complex Source: MGI
- terminal bouton Source: ParkinsonsUK-UCL
- vacuolar membrane Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219058 | 1 – 295 | Alpha-soluble NSF attachment proteinAdd BLAST | 295 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Modified residuei | 29 | PhosphoserineBy similarity | 1 | |
Modified residuei | 195 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P54921 |
PaxDbi | P54921 |
PRIDEi | P54921 |
PTM databases
iPTMneti | P54921 |
PhosphoSitePlusi | P54921 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000001494, Expressed in cerebellum and 21 other tissues |
Genevisiblei | P54921, RN |
Interactioni
Subunit structurei
Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2 (PubMed:11931741).
Found in a complex with VAMP8 (PubMed:9614193).
Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha (By similarity).
Interacts with VTI1A (By similarity).
Interacts with STX12 (PubMed:9507000).
Interacts with GNA12 (via N-terminus); the interaction promotes CDH5 localization to plasma membrane (By similarity).
By similarity3 PublicationsGO - Molecular functioni
- SNARE binding Source: ParkinsonsUK-UCL
- syntaxin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 250832, 3 interactors |
CORUMi | P54921 |
DIPi | DIP-37030N |
IntActi | P54921, 7 interactors |
STRINGi | 10116.ENSRNOP00000002044 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SNAP family.Curated
Phylogenomic databases
eggNOGi | KOG1586, Eukaryota |
GeneTreei | ENSGT00390000005826 |
HOGENOMi | CLU_046329_0_2_1 |
InParanoidi | P54921 |
OMAi | CHFCIDS |
OrthoDBi | 1191204at2759 |
PhylomeDBi | P54921 |
TreeFami | TF316547 |
Family and domain databases
CDDi | cd15832, SNAP, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR000744, NSF_attach IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR13768, PTHR13768, 1 hit |
PRINTSi | PR00448, NSFATTACHMNT |
SUPFAMi | SSF48452, SSF48452, 1 hit |
i Sequence
Sequence statusi: Complete.
P54921-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTSGKQAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDVEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAAQLEQYQK AIDIYEQVGT SAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89968 mRNA Translation: CAA62005.1 BC063156 mRNA Translation: AAH63156.1 |
PIRi | S58285 |
RefSeqi | NP_542152.1, NM_080585.1 |
Genome annotation databases
Ensembli | ENSRNOT00000002044; ENSRNOP00000002044; ENSRNOG00000001494 |
GeneIDi | 140673 |
KEGGi | rno:140673 |
UCSCi | RGD:620855, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89968 mRNA Translation: CAA62005.1 BC063156 mRNA Translation: AAH63156.1 |
PIRi | S58285 |
RefSeqi | NP_542152.1, NM_080585.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J96 | electron microscopy | 7.60 | G/H/I/J | 1-295 | [»] | |
3J97 | electron microscopy | 7.80 | G/H/I/J | 1-295 | [»] | |
3J98 | electron microscopy | 8.40 | G/H/I/J | 1-295 | [»] | |
3J99 | electron microscopy | 8.20 | G/H/I/J | 1-295 | [»] | |
6MDM | electron microscopy | 4.40 | K/L | 1-295 | [»] | |
6MDN | electron microscopy | 4.40 | K/L | 1-278 | [»] | |
SMRi | P54921 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250832, 3 interactors |
CORUMi | P54921 |
DIPi | DIP-37030N |
IntActi | P54921, 7 interactors |
STRINGi | 10116.ENSRNOP00000002044 |
PTM databases
iPTMneti | P54921 |
PhosphoSitePlusi | P54921 |
Proteomic databases
jPOSTi | P54921 |
PaxDbi | P54921 |
PRIDEi | P54921 |
Genome annotation databases
Ensembli | ENSRNOT00000002044; ENSRNOP00000002044; ENSRNOG00000001494 |
GeneIDi | 140673 |
KEGGi | rno:140673 |
UCSCi | RGD:620855, rat |
Organism-specific databases
CTDi | 8775 |
RGDi | 620855, Napa |
Phylogenomic databases
eggNOGi | KOG1586, Eukaryota |
GeneTreei | ENSGT00390000005826 |
HOGENOMi | CLU_046329_0_2_1 |
InParanoidi | P54921 |
OMAi | CHFCIDS |
OrthoDBi | 1191204at2759 |
PhylomeDBi | P54921 |
TreeFami | TF316547 |
Enzyme and pathway databases
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-432722, Golgi Associated Vesicle Biogenesis R-RNO-6807878, COPI-mediated anterograde transport R-RNO-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-RNO-6811438, Intra-Golgi traffic R-RNO-6811440, Retrograde transport at the Trans-Golgi-Network |
Miscellaneous databases
PROi | PR:P54921 |
Gene expression databases
Bgeei | ENSRNOG00000001494, Expressed in cerebellum and 21 other tissues |
Genevisiblei | P54921, RN |
Family and domain databases
CDDi | cd15832, SNAP, 1 hit |
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR000744, NSF_attach IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR13768, PTHR13768, 1 hit |
PRINTSi | PR00448, NSFATTACHMNT |
SUPFAMi | SSF48452, SSF48452, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SNAA_RAT | |
Accessioni | P54921Primary (citable) accession number: P54921 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 15, 1999 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families