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Protein

Alpha-soluble NSF attachment protein

Gene

NAPA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed:15980433).Curated1 Publication

GO - Molecular functioni

  • protein-containing complex binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: ParkinsonsUK-UCL
  • soluble NSF attachment protein activity Source: GO_Central
  • syntaxin binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-204005 COPII-mediated vesicle transport
R-HSA-432722 Golgi Associated Vesicle Biogenesis
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-6811438 Intra-Golgi traffic
R-HSA-6811440 Retrograde transport at the Trans-Golgi-Network

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Gene namesi
Name:NAPA
Synonyms:SNAPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000105402.7
HGNCiHGNC:7641 NAPA
MIMi603215 gene
neXtProtiNX_P54920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi122K → A: Does not affect interaction with GNA12. 1 Publication1
Mutagenesisi140K → A: Increases interaction with GNA12. 1 Publication1

Organism-specific databases

DisGeNETi8775
OpenTargetsiENSG00000105402
PharmGKBiPA31443

Polymorphism and mutation databases

BioMutaiNAPA
DMDMi116242794

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190561 – 295Alpha-soluble NSF attachment proteinAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei26PhosphoserineBy similarity1
Modified residuei29PhosphoserineCombined sources1
Modified residuei195PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP54920
MaxQBiP54920
PaxDbiP54920
PeptideAtlasiP54920
PRIDEiP54920
ProteomicsDBi56747
TopDownProteomicsiP54920

2D gel databases

OGPiP54920

PTM databases

iPTMnetiP54920
PhosphoSitePlusiP54920
SwissPalmiP54920

Miscellaneous databases

PMAP-CutDBiP54920

Expressioni

Gene expression databases

BgeeiENSG00000105402 Expressed in 94 organ(s), highest expression level in cerebellum
CleanExiHS_NAPA
ExpressionAtlasiP54920 baseline and differential
GenevisibleiP54920 HS

Organism-specific databases

HPAiCAB010100
HPA046149
HPA050196

Interactioni

Subunit structurei

Interacts with PRKCABP, and disrupts the interaction between GRIA2 and PRKCABP, leading to the internalization of GRIA2 (By similarity). Found in a complex with VAMP8 (By similarity). Component of a SNARE-like complex that contains at least ZW10, USE1L, RINT1, STX18 and NAPA/SNAP-alpha (PubMed:15029241). Interacts with VTI1A (By similarity). Interacts with STX12 (By similarity). Interacts with GNA12 (via N-terminus); the interaction promotes CDH5 localization to plasma membrane (PubMed:15980433).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114305, 54 interactors
IntActiP54920, 16 interactors
MINTiP54920
STRINGi9606.ENSP00000263354

Structurei

3D structure databases

ProteinModelPortaliP54920
SMRiP54920
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

eggNOGiKOG1586 Eukaryota
ENOG410XPQ6 LUCA
GeneTreeiENSGT00390000005826
HOGENOMiHOG000165015
HOVERGENiHBG001325
InParanoidiP54920
KOiK15296
OMAiYSVKEYW
OrthoDBiEOG091G0KSY
PhylomeDBiP54920
TreeFamiTF316547

Family and domain databases

CDDicd15832 SNAP, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR000744 NSF_attach
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR13768 PTHR13768, 1 hit
PRINTSiPR00448 NSFATTACHMNT
SUPFAMiSSF48452 SSF48452, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

P54920-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN
60 70 80 90 100
MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA
110 120 130 140 150
INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD
160 170 180 190 200
YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY
210 220 230 240 250
SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA
260 270 280 290
HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
Length:295
Mass (Da):33,233
Last modified:October 17, 2006 - v3
Checksum:i7704A619A441E609
GO

Computationally mapped potential isoform sequencesi

There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R0Y2M0R0Y2_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
256Annotation score:
M0R2M1M0R2M1_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
268Annotation score:
M0R027M0R027_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
102Annotation score:
M0R058M0R058_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
137Annotation score:
M0R213M0R213_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
83Annotation score:
M0R0I4M0R0I4_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
139Annotation score:
M0R031M0R031_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
74Annotation score:
M0QZN5M0QZN5_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
60Annotation score:
M0QZM9M0QZM9_HUMAN
Alpha-soluble NSF attachment protei...
NAPA
35Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195S → T in AAC80170 (PubMed:9269766).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39412 mRNA Translation: AAC80170.1
BT019568 mRNA Translation: AAV38375.1
AK292244 mRNA Translation: BAF84933.1
CH471126 Genomic DNA Translation: EAW57491.1
BC001165 mRNA Translation: AAH01165.1
BC007432 mRNA Translation: AAH07432.1
BC028234 mRNA Translation: AAH28234.1
BC091511 mRNA Translation: AAH91511.1
CCDSiCCDS12702.1
PIRiG02238
RefSeqiNP_003818.2, NM_003827.3
UniGeneiHs.126938
Hs.730799
Hs.744881

Genome annotation databases

EnsembliENST00000263354; ENSP00000263354; ENSG00000105402
GeneIDi8775
KEGGihsa:8775
UCSCiuc002pha.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39412 mRNA Translation: AAC80170.1
BT019568 mRNA Translation: AAV38375.1
AK292244 mRNA Translation: BAF84933.1
CH471126 Genomic DNA Translation: EAW57491.1
BC001165 mRNA Translation: AAH01165.1
BC007432 mRNA Translation: AAH07432.1
BC028234 mRNA Translation: AAH28234.1
BC091511 mRNA Translation: AAH91511.1
CCDSiCCDS12702.1
PIRiG02238
RefSeqiNP_003818.2, NM_003827.3
UniGeneiHs.126938
Hs.730799
Hs.744881

3D structure databases

ProteinModelPortaliP54920
SMRiP54920
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114305, 54 interactors
IntActiP54920, 16 interactors
MINTiP54920
STRINGi9606.ENSP00000263354

PTM databases

iPTMnetiP54920
PhosphoSitePlusiP54920
SwissPalmiP54920

Polymorphism and mutation databases

BioMutaiNAPA
DMDMi116242794

2D gel databases

OGPiP54920

Proteomic databases

EPDiP54920
MaxQBiP54920
PaxDbiP54920
PeptideAtlasiP54920
PRIDEiP54920
ProteomicsDBi56747
TopDownProteomicsiP54920

Protocols and materials databases

DNASUi8775
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263354; ENSP00000263354; ENSG00000105402
GeneIDi8775
KEGGihsa:8775
UCSCiuc002pha.3 human

Organism-specific databases

CTDi8775
DisGeNETi8775
EuPathDBiHostDB:ENSG00000105402.7
GeneCardsiNAPA
H-InvDBiHIX0137469
HGNCiHGNC:7641 NAPA
HPAiCAB010100
HPA046149
HPA050196
MIMi603215 gene
neXtProtiNX_P54920
OpenTargetsiENSG00000105402
PharmGKBiPA31443
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1586 Eukaryota
ENOG410XPQ6 LUCA
GeneTreeiENSGT00390000005826
HOGENOMiHOG000165015
HOVERGENiHBG001325
InParanoidiP54920
KOiK15296
OMAiYSVKEYW
OrthoDBiEOG091G0KSY
PhylomeDBiP54920
TreeFamiTF316547

Enzyme and pathway databases

ReactomeiR-HSA-204005 COPII-mediated vesicle transport
R-HSA-432722 Golgi Associated Vesicle Biogenesis
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-6811438 Intra-Golgi traffic
R-HSA-6811440 Retrograde transport at the Trans-Golgi-Network

Miscellaneous databases

ChiTaRSiNAPA human
GeneWikiiNAPA_(gene)
GenomeRNAii8775
PMAP-CutDBiP54920
PROiPR:P54920
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105402 Expressed in 94 organ(s), highest expression level in cerebellum
CleanExiHS_NAPA
ExpressionAtlasiP54920 baseline and differential
GenevisibleiP54920 HS

Family and domain databases

CDDicd15832 SNAP, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR000744 NSF_attach
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR13768 PTHR13768, 1 hit
PRINTSiPR00448 NSFATTACHMNT
SUPFAMiSSF48452 SSF48452, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNAA_HUMAN
AccessioniPrimary (citable) accession number: P54920
Secondary accession number(s): A8K879, Q96IK3, Q9BVJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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