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UniProtKB - P54887 (P5CS1_ARATH)
Protein
Delta-1-pyrroline-5-carboxylate synthase A
Gene
P5CSA
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.
Catalytic activityi
: L-proline biosynthesis Pathwayi
This protein is involved in step 1 and 2 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate. This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 60 | SubstrateBy similarity | 1 | |
Binding sitei | 157 | SubstrateBy similarity | 1 | |
Binding sitei | 176 | Substrate; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 196 – 197 | ATPBy similarity | 2 | |
Nucleotide bindingi | 236 – 242 | ATPBy similarity | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- delta1-pyrroline-5-carboxylate synthetase activity Source: TAIR
- glutamate 5-kinase activity Source: UniProtKB-EC
- glutamate-5-semialdehyde dehydrogenase activity Source: GO_Central
GO - Biological processi
- hyperosmotic salinity response Source: TAIR
- L-proline biosynthetic process Source: UniProtKB-UniPathway
- pollen development Source: TAIR
- proline biosynthetic process Source: TAIR
- response to oxidative stress Source: TAIR
- response to salt stress Source: TAIR
- response to water deprivation Source: TAIR
- root development Source: TAIR
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | Amino-acid biosynthesis, Proline biosynthesis |
Ligand | ATP-binding, NADP, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G39800-MONOMER |
UniPathwayi | UPA00098;UER00359 UPA00098;UER00360 |
Names & Taxonomyi
Protein namesi | Recommended name: Delta-1-pyrroline-5-carboxylate synthase AShort name: P5CS A Including the following 2 domains: |
Gene namesi | Name:P5CSA Synonyms:P5CS1 Ordered Locus Names:At2g39800 ORF Names:T5I7.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G39800 |
TAIRi | locus:2063907, AT2G39800 |
Subcellular locationi
Chloroplast
- chloroplast Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
Other locations
- cytoplasm Source: TAIR
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109772 | 1 – 717 | Delta-1-pyrroline-5-carboxylate synthase AAdd BLAST | 717 |
Proteomic databases
PaxDbi | P54887 |
PTM databases
iPTMneti | P54887 |
Expressioni
Gene expression databases
ExpressionAtlasi | P54887, baseline and differential |
Genevisiblei | P54887, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 3904, 6 interactors |
IntActi | P54887, 6 interactors |
STRINGi | 3702.AT2G39800.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 296 | Glutamate 5-kinaseAdd BLAST | 296 | |
Regioni | 297 – 717 | Gamma-glutamyl phosphate reductaseAdd BLAST | 421 |
Sequence similaritiesi
In the N-terminal section; belongs to the glutamate 5-kinase family.Curated
In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1154, Eukaryota KOG4165, Eukaryota |
InParanoidi | P54887 |
PhylomeDBi | P54887 |
Family and domain databases
CDDi | cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit MF_00456, ProB, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR001048, Asp/Glu/Uridylate_kinase IPR020593, G-glutamylP_reductase_CS IPR001057, Glu/AcGlu_kinase IPR005715, Glu_5kinase/COase_Synthase IPR019797, Glutamate_5-kinase_CS IPR000965, GPR_dom IPR005766, P5_carboxy_syn |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036429, P5C_syn, 1 hit |
PRINTSi | PR00474, GLU5KINASE |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01092, P5CS, 1 hit TIGR00407, proA, 1 hit TIGR01027, proB, 1 hit |
PROSITEi | View protein in PROSITE PS00902, GLUTAMATE_5_KINASE, 1 hit PS01223, PROA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P54887-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEELDRSRAF ARDVKRIVVK VGTAVVTGKG GRLALGRLGA LCEQLAELNS
60 70 80 90 100
DGFEVILVSS GAVGLGRQRL RYRQLVNSSF ADLQKPQTEL DGKACAGVGQ
110 120 130 140 150
SSLMAYYETM FDQLDVTAAQ LLVNDSSFRD KDFRKQLNET VKSMLDLRVI
160 170 180 190 200
PIFNENDAIS TRRAPYQDSS GIFWDNDSLA ALLALELKAD LLILLSDVEG
210 220 230 240 250
LYTGPPSDPN SKLIHTFVKE KHQDEITFGD KSRLGRGGMT AKVKAAVNAA
260 270 280 290 300
YAGIPVIITS GYSAENIDKV LRGLRVGTLF HQDARLWAPI TDSNARDMAV
310 320 330 340 350
AARESSRKLQ ALSSEDRKKI LLDIADALEA NVTTIKAENE LDVASAQEAG
360 370 380 390 400
LEESMVARLV MTPGKISSLA ASVRKLADME DPIGRVLKKT EVADGLVLEK
410 420 430 440 450
TSSPLGVLLI VFESRPDALV QIASLAIRSG NGLLLKGGKE ARRSNAILHK
460 470 480 490 500
VITDAIPETV GGKLIGLVTS REEIPDLLKL DDVIDLVIPR GSNKLVTQIK
510 520 530 540 550
NTTKIPVLGH ADGICHVYVD KACDTDMAKR IVSDAKLDYP AACNAMETLL
560 570 580 590 600
VHKDLEQNAV LNELIFALQS NGVTLYGGPR ASKILNIPEA RSFNHEYCAK
610 620 630 640 650
ACTVEVVEDV YGAIDHIHRH GSAHTDCIVT EDHEVAELFL RQVDSAAVFH
660 670 680 690 700
NASTRFSDGF RFGLGAEVGV STGRIHARGP VGVEGLLTTR WIMRGKGQVV
710
DGDNGIVYTH QDIPIQA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4IFZ9 | F4IFZ9_ARATH | Delta-1-pyrroline-5-carboxylate syn... | P5CS1 ATP5CS, At2g39800, T5I7.10, T5I7_10 | 714 | Annotation score: | ||
B9DFG0 | B9DFG0_ARATH | AT2G39800 protein | P5CS1 ATP5CS, At2g39800, T5I7.10, T5I7_10 | 614 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 | C → F in BAA06864 (PubMed:7773306).Curated | 1 | |
Sequence conflicti | 54 | E → K in BAA06864 (PubMed:7773306).Curated | 1 | |
Sequence conflicti | 381 | D → E in BAA06864 (PubMed:7773306).Curated | 1 | |
Sequence conflicti | 467 | L → F in BAA06864 (PubMed:7773306).Curated | 1 | |
Sequence conflicti | 676 | H → Y in BAA06864 (PubMed:7773306).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89414 Genomic DNA Translation: CAA61593.1 X86777 mRNA Translation: CAA60446.1 X87330 mRNA Translation: CAA60740.1 D32138 mRNA Translation: BAA06864.1 AC003000 Genomic DNA Translation: AAB87129.1 CP002685 Genomic DNA Translation: AEC09729.1 CP002685 Genomic DNA Translation: AEC09730.1 AF424633 mRNA Translation: AAL11626.1 AY113046 mRNA Translation: AAM47354.1 AY150430 mRNA Translation: AAN12972.1 |
PIRi | S66637 T50685 |
RefSeqi | NP_001189714.1, NM_001202785.1 [P54887-1] NP_181510.1, NM_129539.2 [P54887-1] |
Genome annotation databases
EnsemblPlantsi | AT2G39800.1; AT2G39800.1; AT2G39800 [P54887-1] AT2G39800.4; AT2G39800.4; AT2G39800 [P54887-1] |
GeneIDi | 818566 |
Gramenei | AT2G39800.1; AT2G39800.1; AT2G39800 [P54887-1] AT2G39800.4; AT2G39800.4; AT2G39800 [P54887-1] |
KEGGi | ath:AT2G39800 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89414 Genomic DNA Translation: CAA61593.1 X86777 mRNA Translation: CAA60446.1 X87330 mRNA Translation: CAA60740.1 D32138 mRNA Translation: BAA06864.1 AC003000 Genomic DNA Translation: AAB87129.1 CP002685 Genomic DNA Translation: AEC09729.1 CP002685 Genomic DNA Translation: AEC09730.1 AF424633 mRNA Translation: AAL11626.1 AY113046 mRNA Translation: AAM47354.1 AY150430 mRNA Translation: AAN12972.1 |
PIRi | S66637 T50685 |
RefSeqi | NP_001189714.1, NM_001202785.1 [P54887-1] NP_181510.1, NM_129539.2 [P54887-1] |
3D structure databases
AlphaFoldDBi | P54887 |
SMRi | P54887 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 3904, 6 interactors |
IntActi | P54887, 6 interactors |
STRINGi | 3702.AT2G39800.1 |
PTM databases
iPTMneti | P54887 |
Proteomic databases
PaxDbi | P54887 |
Genome annotation databases
EnsemblPlantsi | AT2G39800.1; AT2G39800.1; AT2G39800 [P54887-1] AT2G39800.4; AT2G39800.4; AT2G39800 [P54887-1] |
GeneIDi | 818566 |
Gramenei | AT2G39800.1; AT2G39800.1; AT2G39800 [P54887-1] AT2G39800.4; AT2G39800.4; AT2G39800 [P54887-1] |
KEGGi | ath:AT2G39800 |
Organism-specific databases
Araporti | AT2G39800 |
TAIRi | locus:2063907, AT2G39800 |
Phylogenomic databases
eggNOGi | KOG1154, Eukaryota KOG4165, Eukaryota |
InParanoidi | P54887 |
PhylomeDBi | P54887 |
Enzyme and pathway databases
UniPathwayi | UPA00098;UER00359 UPA00098;UER00360 |
BioCyci | ARA:AT2G39800-MONOMER |
Miscellaneous databases
PROi | PR:P54887 |
Gene expression databases
ExpressionAtlasi | P54887, baseline and differential |
Genevisiblei | P54887, AT |
Family and domain databases
CDDi | cd07079, ALDH_F18-19_ProA-GPR, 1 hit |
Gene3Di | 3.40.1160.10, 1 hit 3.40.309.10, 1 hit 3.40.605.10, 1 hit |
HAMAPi | MF_00412, ProA, 1 hit MF_00456, ProB, 1 hit |
InterProi | View protein in InterPro IPR036393, AceGlu_kinase-like_sf IPR016161, Ald_DH/histidinol_DH IPR016163, Ald_DH_C IPR016162, Ald_DH_N IPR015590, Aldehyde_DH_dom IPR001048, Asp/Glu/Uridylate_kinase IPR020593, G-glutamylP_reductase_CS IPR001057, Glu/AcGlu_kinase IPR005715, Glu_5kinase/COase_Synthase IPR019797, Glutamate_5-kinase_CS IPR000965, GPR_dom IPR005766, P5_carboxy_syn |
Pfami | View protein in Pfam PF00696, AA_kinase, 1 hit PF00171, Aldedh, 1 hit |
PIRSFi | PIRSF036429, P5C_syn, 1 hit |
PRINTSi | PR00474, GLU5KINASE |
SUPFAMi | SSF53633, SSF53633, 1 hit SSF53720, SSF53720, 1 hit |
TIGRFAMsi | TIGR01092, P5CS, 1 hit TIGR00407, proA, 1 hit TIGR01027, proB, 1 hit |
PROSITEi | View protein in PROSITE PS00902, GLUTAMATE_5_KINASE, 1 hit PS01223, PROA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | P5CS1_ARATH | |
Accessioni | P54887Primary (citable) accession number: P54887 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families