UniProtKB - P54867 (SLG1_YEAST)
Protein
Protein SLG1
Gene
SLG1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plays a role during G1 to regulate entering or exiting the cell cycle. Involved in stress responses. Has a role in cell wall integrity signaling. Activates ROM1 or ROM2 catalyzed guanine nucleotide exchange toward RHO1. Important regulator of the actin cytoskeleton rearrangements in conditions of cell wall expansion and membrane stretching. Specifically required for the actin reorganization induced by hypo-osmotic shock. Multicopy suppressor of 1,3-beta-glucan synthase (GS). Activates GS upstream of RHO1. Acts positively on the PKC1-MAPK pathway. Activates transiently SLT2 during alkaline stress, which leads to an increase in the expression of several specific genes.5 Publications
Miscellaneous
Present with 664 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- transmembrane signaling receptor activity Source: SGD
GO - Biological processi
- actin cytoskeleton organization Source: SGD
- autophagy of peroxisome Source: SGD
- cell cycle Source: UniProtKB-KW
- establishment of cell polarity Source: SGD
- fungal-type cell wall organization Source: SGD
- positive regulation of endocytosis Source: SGD
- response to heat Source: SGD
- response to osmotic stress Source: SGD
- Rho protein signal transduction Source: SGD
Keywordsi
Biological process | Cell cycle, Cell wall biogenesis/degradation, Stress response |
Names & Taxonomyi
Protein namesi | Recommended name: Protein SLG1Alternative name(s): Cell wall integrity and stress response component 1 Synthetic lethal with GAP protein 1 |
Gene namesi | Name:SLG1 Synonyms:WSC1 Ordered Locus Names:YOR008C ORF Names:UNF378 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005534, SLG1 |
VEuPathDBi | FungiDB:YOR008C |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Vacuole
- fungal-type vacuole Source: SGD
Other locations
- cellular bud neck Source: SGD
- integral component of membrane Source: UniProtKB-KW
- mating projection tip Source: SGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 264 | ExtracellularSequence analysisAdd BLAST | 243 | |
Transmembranei | 265 – 285 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 286 – 378 | CytoplasmicSequence analysisAdd BLAST | 93 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Lack of SLG1 leads to a significant defect of 1,3-beta-glucan synthesis and confers sensitivity to caffeine, SDS, Calcofluor and alkaline pH stress. Deletion of residues 21-256 results in non-functional protein, removal of residues 21-110 yields a protein still able to confer some tolerance to alkaline stress, and deletion of residues 116-256 has no effect on the function.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 303 | Y → A: Sensitive to alkali. Fails to restore normal levels of SLT2 phosphorylation upon alkaline stress. 1 Publication | 1 | |
Mutagenesisi | 319 | S → A: No phosphorylation; when associated with A-320. 1 Publication | 1 | |
Mutagenesisi | 320 | S → A: No phosphorylation; when associated with A-319. 1 Publication | 1 | |
Mutagenesisi | 322 | S → A: No phosphorylation; when associated with A-323. 1 Publication | 1 | |
Mutagenesisi | 323 | S → A: No phosphorylation; when associated with A-322. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000041483 | 22 – 378 | Protein SLG1Add BLAST | 357 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 65 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 331 | PhosphoserineCombined sources | 1 | |
Modified residuei | 353 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Glycosylated. Phosphorylated. Phosphorylation serves a negative regulatory role.2 Publications
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
MaxQBi | P54867 |
PaxDbi | P54867 |
PRIDEi | P54867 |
PTM databases
iPTMneti | P54867 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34412, 224 interactors |
DIPi | DIP-4196N |
IntActi | P54867, 2 interactors |
STRINGi | 4932.YOR008C |
Miscellaneous databases
RNActi | P54867, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 110 | WSCPROSITE-ProRule annotationAdd BLAST | 89 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 125 – 255 | Thr-richAdd BLAST | 131 |
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4157, Eukaryota |
HOGENOMi | CLU_024893_1_1_1 |
InParanoidi | P54867 |
OMAi | IKPVEYP |
Family and domain databases
InterProi | View protein in InterPro IPR002889, WSC_carb-bd |
Pfami | View protein in Pfam PF01822, WSC, 1 hit |
SMARTi | View protein in SMART SM00321, WSC, 1 hit |
PROSITEi | View protein in PROSITE PS51212, WSC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P54867-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRPNKTSLLL ALLSILSQAN AYEYVNCFSS LPSDFSKADS YNWQSSSHCN
60 70 80 90 100
SECSAKGASY FALYNHSECY CGDTNPSGSE STSSSCNTYC FGYSSEMCGG
110 120 130 140 150
EDAYSVYQLD SDTNSNSISS SDSSTESTSA SSSTTSSTTS STTSTTSSTT
160 170 180 190 200
SSTTSSMASS STVQNSPEST QAAASISTSQ SSSTVTSESS LTSDTLATSS
210 220 230 240 250
TSSQSQDATS IIYSTTFHTE GGSTIFVTNT ITASAQNSGS ATGTAGSDST
260 270 280 290 300
SGSKTHKKKA NVGAIVGGVV GGVVGAVAIA LCILLIVRHI NMKREQDRME
310 320 330 340 350
KEYQEAIKPV EYPDKLYASS FSSNHGPSSG SFEEEHTKGQ TDINPFDDSR
360 370
RISNGTFING GPGGKNNVLT VVNPDEAD
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39481 Genomic DNA Translation: AAA85862.1 U43491 Genomic DNA Translation: AAC49488.1 Z74916 Genomic DNA Translation: CAA99196.1 BK006948 Genomic DNA Translation: DAA10790.1 |
PIRi | S61992 |
RefSeqi | NP_014650.1, NM_001183427.1 |
Genome annotation databases
EnsemblFungii | YOR008C_mRNA; YOR008C; YOR008C |
GeneIDi | 854170 |
KEGGi | sce:YOR008C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U39481 Genomic DNA Translation: AAA85862.1 U43491 Genomic DNA Translation: AAC49488.1 Z74916 Genomic DNA Translation: CAA99196.1 BK006948 Genomic DNA Translation: DAA10790.1 |
PIRi | S61992 |
RefSeqi | NP_014650.1, NM_001183427.1 |
3D structure databases
SMRi | P54867 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34412, 224 interactors |
DIPi | DIP-4196N |
IntActi | P54867, 2 interactors |
STRINGi | 4932.YOR008C |
PTM databases
iPTMneti | P54867 |
Proteomic databases
MaxQBi | P54867 |
PaxDbi | P54867 |
PRIDEi | P54867 |
Genome annotation databases
EnsemblFungii | YOR008C_mRNA; YOR008C; YOR008C |
GeneIDi | 854170 |
KEGGi | sce:YOR008C |
Organism-specific databases
SGDi | S000005534, SLG1 |
VEuPathDBi | FungiDB:YOR008C |
Phylogenomic databases
eggNOGi | KOG4157, Eukaryota |
HOGENOMi | CLU_024893_1_1_1 |
InParanoidi | P54867 |
OMAi | IKPVEYP |
Miscellaneous databases
PROi | PR:P54867 |
RNActi | P54867, protein |
Family and domain databases
InterProi | View protein in InterPro IPR002889, WSC_carb-bd |
Pfami | View protein in Pfam PF01822, WSC, 1 hit |
SMARTi | View protein in SMART SM00321, WSC, 1 hit |
PROSITEi | View protein in PROSITE PS51212, WSC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SLG1_YEAST | |
Accessioni | P54867Primary (citable) accession number: P54867 Secondary accession number(s): D6W274 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names