UniProtKB - P54843 (MAF_MOUSE)
Transcription factor Maf
Maf
Functioni
Acts as a transcriptional activator or repressor. When overexpressed, represses anti-oxidant response element (ARE)-mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters (By similarity).
Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5'-[GT]G[GC]N[GT]NCTCAGNN-3' in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells. It may interact with additional basic-zipper proteins that determine a subtype of Maf-responsive element binding.
By similarity12 PublicationsGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- double-stranded DNA binding Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- cell development Source: MGI
- inner ear development Source: MGI
- lens development in camera-type eye Source: MGI
- lens fiber cell differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of chondrocyte differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor MafAlternative name(s): Proto-oncogene c-Maf V-maf musculoaponeurotic fibrosarcoma oncogene homolog |
Gene namesi | Name:Maf Synonyms:Maf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96909, Maf |
VEuPathDBi | HostDB:ENSMUSG00000055435 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
- RNA polymerase II transcription regulator complex Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 15 | S → A: Inhibition on transcriptional activation on CD13 proximal promoter in endothelial cells. 1 Publication | 1 | |
Mutagenesisi | 70 | S → A: No effect on transcriptional activation on CD13 proximal promoter. Increases liver specific transactivation on the IL-4 promoter. 1 Publication | 1 | |
Mutagenesisi | 334 | L → P: Abolishes interaction with USF2. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogene, Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076492 | 1 – 370 | Transcription factor MafAdd BLAST | 370 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 29 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 33 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 328 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P54843 |
PaxDbi | P54843 |
ProteomicsDBi | 287293 [P54843-1] 287294 [P54843-2] 287295 [P54843-3] |
PTM databases
iPTMneti | P54843 |
PhosphoSitePlusi | P54843 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000055435, Expressed in lens of camera-type eye and 290 other tissues |
Genevisiblei | P54843, MM |
Interactioni
Subunit structurei
Homodimer or heterodimer with other bHLH-Zip transcription factors. Binds DNA as a homodimer or as a heterodimer. Heterotetramer of two MAF and two USF2.
Interacts with PAX6; the interaction is direct.
Interacts with MYB; interaction takes place weakly in normal T-cells and increases in T-cells following stimulation through the TCR engagement.
Interacts with MYB; the ternary complex formed with MYB and the CD13 promoter is regulated in response to differentiating signals.
Interacts with USF2; the interaction inhibits its DNA-binding activity on the L7 promoter.
Interacts with CREBBP, EP300 and ETS1 (By similarity).
By similarityBinary interactionsi
P54843
With | #Exp. | IntAct |
---|---|---|
Smarca5 [Q91ZW3] | 2 | EBI-3842521,EBI-927547 |
GO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201281, 2 interactors |
IntActi | P54843, 2 interactors |
MINTi | P54843 |
STRINGi | 10090.ENSMUSP00000104732 |
Miscellaneous databases
RNActi | P54843, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 285 – 348 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 84 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 126 – 370 | Represses ARE-mediated transcriptionBy similarityAdd BLAST | 245 | |
Regioni | 175 – 244 | DisorderedSequence analysisAdd BLAST | 70 | |
Regioni | 285 – 310 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 313 – 334 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 179 – 193 | Basic residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000161531 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54843 |
OMAi | LMSEITW |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54843 |
TreeFami | TF325689 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR028573, Maf/V-MAF IPR013592, Maf_TF_N IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF9, PTHR10129:SF9, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASELAMNNS DLPTSPLAME YVNDFDLMKF EVKKEPVETD RIISQCGRLI
60 70 80 90 100
AGGSLSSTPM STPCSSVPPS PSFSAPSPGS GSEQKAHLED YYWMTGYPQQ
110 120 130 140 150
LNPEALGFSP EDAVEALISN SHQLQGGFDG YARGAQQLAA AAGAGAGASL
160 170 180 190 200
GGSGEEMGPA AAVVSAVIAA AAAQSGAAPH YHHHHHHAAG HHHHPTAGAP
210 220 230 240 250
GAAGGASASA SGAGGAGGGG PASAGGGGGG GGGGGTAGAG GALHPHHAAG
260 270 280 290 300
GLHFDDRFSD EQLVTMSVRE LNRQLRGVSK EEVIRLKQKR RTLKNRGYAQ
310 320 330 340 350
SCRFKRVQQR HVLESEKNQL LQQVDHLKQE ISRLVRERDA YKEKYEKLVS
360 370
NGFRENGSSS DNPSSPEFFM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 202 | A → T in AAB32820 (PubMed:7799931).Curated | 1 | |
Sequence conflicti | 208 – 211 | ASAS → SSSN in AAB32820 (PubMed:7799931).Curated | 4 | |
Sequence conflicti | 223 – 224 | SA → NT in AAB32820 (PubMed:7799931).Curated | 2 | |
Sequence conflicti | 231 | G → D in AAB32820 (PubMed:7799931).Curated | 1 | |
Sequence conflicti | 248 | A → S in AAB32820 (PubMed:7799931).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036408 | 370 | M → MCVCVCALFIL in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_036409 | 370 | M → MYPRDSSTSVM in isoform 3. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S74567 mRNA Translation: AAB32820.1 AY560005 mRNA Translation: AAY81957.1 AK132165 mRNA Translation: BAE21007.1 AC113301 Genomic DNA No translation available. |
CCDSi | CCDS40486.1 [P54843-1] |
RefSeqi | NP_001020748.2, NM_001025577.2 [P54843-1] |
Genome annotation databases
Ensembli | ENSMUST00000069009; ENSMUSP00000067704; ENSMUSG00000055435 [P54843-3] ENSMUST00000109104; ENSMUSP00000104732; ENSMUSG00000055435 [P54843-1] |
GeneIDi | 17132 |
KEGGi | mmu:17132 |
UCSCi | uc009noe.2, mouse [P54843-3] uc009nof.1, mouse [P54843-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S74567 mRNA Translation: AAB32820.1 AY560005 mRNA Translation: AAY81957.1 AK132165 mRNA Translation: BAE21007.1 AC113301 Genomic DNA No translation available. |
CCDSi | CCDS40486.1 [P54843-1] |
RefSeqi | NP_001020748.2, NM_001025577.2 [P54843-1] |
3D structure databases
AlphaFoldDBi | P54843 |
SMRi | P54843 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201281, 2 interactors |
IntActi | P54843, 2 interactors |
MINTi | P54843 |
STRINGi | 10090.ENSMUSP00000104732 |
PTM databases
iPTMneti | P54843 |
PhosphoSitePlusi | P54843 |
Proteomic databases
MaxQBi | P54843 |
PaxDbi | P54843 |
ProteomicsDBi | 287293 [P54843-1] 287294 [P54843-2] 287295 [P54843-3] |
Protocols and materials databases
Antibodypediai | 4143, 497 antibodies from 39 providers |
DNASUi | 17132 |
Genome annotation databases
Ensembli | ENSMUST00000069009; ENSMUSP00000067704; ENSMUSG00000055435 [P54843-3] ENSMUST00000109104; ENSMUSP00000104732; ENSMUSG00000055435 [P54843-1] |
GeneIDi | 17132 |
KEGGi | mmu:17132 |
UCSCi | uc009noe.2, mouse [P54843-3] uc009nof.1, mouse [P54843-1] |
Organism-specific databases
CTDi | 4094 |
MGIi | MGI:96909, Maf |
VEuPathDBi | HostDB:ENSMUSG00000055435 |
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000161531 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54843 |
OMAi | LMSEITW |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54843 |
TreeFami | TF325689 |
Miscellaneous databases
BioGRID-ORCSi | 17132, 1 hit in 76 CRISPR screens |
ChiTaRSi | Maf, mouse |
PROi | PR:P54843 |
RNActi | P54843, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055435, Expressed in lens of camera-type eye and 290 other tissues |
Genevisiblei | P54843, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR028573, Maf/V-MAF IPR013592, Maf_TF_N IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF9, PTHR10129:SF9, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAF_MOUSE | |
Accessioni | P54843Primary (citable) accession number: P54843 Secondary accession number(s): Q3V1Z2, Q4QY62 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | February 10, 2009 | |
Last modified: | May 25, 2022 | |
This is version 173 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families