UniProtKB - P54842 (MAFB_RAT)
Transcription factor MafB
Mafb
Functioni
Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context (By similarity).
Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (By similarity).
By similarityGO - Molecular functioni
- DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: RGD
- identical protein binding Source: RGD
- protein-containing complex binding Source: RGD
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- abducens nerve formation Source: UniProtKB
- brain segmentation Source: RGD
- cornified envelope assembly Source: RGD
- fat cell differentiation Source: UniProtKB
- gene expression Source: RGD
- inner ear morphogenesis Source: RGD
- keratinocyte differentiation Source: RGD
- negative regulation of erythrocyte differentiation Source: RGD
- negative regulation of osteoclast differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- protein processing Source: RGD
- regulation of myeloid cell differentiation Source: GO_Central
- regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: RGD
- respiratory gaseous exchange by respiratory system Source: RGD
- rhombomere 5 development Source: RGD
- rhombomere 6 development Source: RGD
- segment specification Source: RGD
- T cell differentiation in thymus Source: RGD
- thymus development Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor MafBShort name: Maf-B Alternative name(s): Transcription factor Maf-1 V-maf musculoaponeurotic fibrosarcoma oncogene homolog B |
Gene namesi | Name:Mafb Synonyms:Maf1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2982, Mafb |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleus Source: RGD
- RNA polymerase II transcription regulator complex Source: RGD
Other locations
- transcription regulator complex Source: RGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076496 | 1 – 323 | Transcription factor MafBAdd BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 32 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 297 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | P54842 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSRNOG00000016037, Expressed in liver and 22 other tissues |
Genevisiblei | P54842, RN |
Interactioni
Subunit structurei
Homodimer or heterodimer with other bHLH-Zip transcription factors. Forms homodimers and heterodimers with FOS, FOSB and FOSL2, but not with JUN proteins (JUN, JUNB and JUND). Binds DNA as a homodimer or a heterodimer.
Interacts with the intracellular cytoplasmic domain of LRP1 (LRPICD); the interaction results in a moderate reduction of MAFB transcriptional potential.
Interacts with PAX6; the interaction is direct.
Interacts with ETS1 and LRP1 (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000021452 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 238 – 301 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 78 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 116 – 209 | DisorderedSequence analysisAdd BLAST | 94 | |
Regioni | 238 – 263 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 266 – 287 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 50 – 78 | Polar residuesSequence analysisAdd BLAST | 29 | |
Compositional biasi | 129 – 143 | Basic residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160486 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54842 |
OMAi | ESHQPLH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54842 |
TreeFami | TF325689 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR013592, Maf_TF_N IPR028571, MafB IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF10, PTHR10129:SF10, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAAELSMGPE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ
60 70 80 90 100
PAGSVSSTPL STPCSSVPSS PSFSPTEQKT HLEDLYWMAS NYQQMNPEAL
110 120 130 140 150
NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV
160 170 180 190 200
THDELGPHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHAAAAATA
210 220 230 240 250
AGSNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG
260 270 280 290 300
YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK
310 320
LANSGFREAG STSDSPSSPE FFL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56241 mRNA Translation: AAB50062.1 |
RefSeqi | NP_062189.1, NM_019316.1 |
Genome annotation databases
Ensembli | ENSRNOT00000021452; ENSRNOP00000021452; ENSRNOG00000016037 |
GeneIDi | 54264 |
KEGGi | rno:54264 |
UCSCi | RGD:2982, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U56241 mRNA Translation: AAB50062.1 |
RefSeqi | NP_062189.1, NM_019316.1 |
3D structure databases
AlphaFoldDBi | P54842 |
SMRi | P54842 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000021452 |
Proteomic databases
PaxDbi | P54842 |
Genome annotation databases
Ensembli | ENSRNOT00000021452; ENSRNOP00000021452; ENSRNOG00000016037 |
GeneIDi | 54264 |
KEGGi | rno:54264 |
UCSCi | RGD:2982, rat |
Organism-specific databases
CTDi | 9935 |
RGDi | 2982, Mafb |
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160486 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54842 |
OMAi | ESHQPLH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54842 |
TreeFami | TF325689 |
Miscellaneous databases
PROi | PR:P54842 |
Gene expression databases
Bgeei | ENSRNOG00000016037, Expressed in liver and 22 other tissues |
Genevisiblei | P54842, RN |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR046347, bZIP_sf IPR013592, Maf_TF_N IPR028571, MafB IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF10, PTHR10129:SF10, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit SSF57959, SSF57959, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAFB_RAT | |
Accessioni | P54842Primary (citable) accession number: P54842 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families