Mafb

Functionⁱ

Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.By similarity8 Publications

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 12701106
- 12798298
protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from mutant phenotypeⁱ
- 12701106
RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: MGI
sequence-specific double-stranded DNA binding Source: MGI
transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding
Source: NTNU_SB
Inferred from mutant phenotypeⁱ
- 12701106
transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

abducens nerve formation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 27181683
brain segmentation
Source: MGI
Inferred from mutant phenotypeⁱ
- 7925021
inner ear morphogenesis
Source: MGI
Inferred from mutant phenotypeⁱ
- 16325169
negative regulation of erythrocyte differentiation Source: MGI
negative regulation of osteoclast differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 19440205
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 12798298
regulation of transcription, DNA-templated Source: MGI
regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 12701106
respiratory gaseous exchange
Source: MGI
Inferred from mutant phenotypeⁱ
- 14513037
rhombomere 5 development
Source: MGI
Inferred from mutant phenotypeⁱ
- 7925021
rhombomere 6 development
Source: MGI
Inferred from mutant phenotypeⁱ
- 7925021
segment specification
Source: MGI
Inferred from mutant phenotypeⁱ
- 12701106
T cell differentiation in thymus
Source: MGI
Inferred from mutant phenotypeⁱ
- 19164599
thymus development
Source: MGI
Inferred from mutant phenotypeⁱ
- 19164599

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor MafB Short name: Maf-B Alternative name(s): Kreisler Segmentation protein Kr Transcription factor Maf-1 V-maf musculoaponeurotic fibrosarcoma oncogene homolog B
Gene namesⁱ	Name:Mafb Synonyms:Krml, Maf1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:104555 Mafb

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice show a defect in frequency of respiratory rhythm with a fatal apnea at birth due to lack of neurons from the preBoetC region. They displayed renal dysgenesis with abnormal podocyte differentiation as well as tubular apoptosis. They show altered actin-dependent macrophage morphology. They show a reduced number of cells expressing insulin and glucagon. Embryos also lack the abducens nerve which normaly innervates the lateral rectus muscle that is involved in eye movement (PubMed:27181683).6 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	32	K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-297. 1 Publication	1
Mutagenesisⁱ	248	N → A: Reduces ability to activate insulin and glucagon gene expression. 1 Publication	1
Mutagenesisⁱ	248	N → S: Loss of transcriptional activity. 1 Publication	1
Mutagenesisⁱ	297	K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-32. 1 Publication	1

Keywords - Diseaseⁱ

Proto-oncogene, Tumor suppressor

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000076495	1 – 323	Transcription factor MafBAdd BLAST		323

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	32	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linkⁱ	297	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationⁱ

Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.1 Publication

Keywords - PTMⁱ

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P54841
PaxDbⁱ	P54841
PeptideAtlas More...PeptideAtlasⁱ	P54841
PRIDEⁱ	P54841

PTM databases

iPTMnetⁱ	P54841
PhosphoSitePlusⁱ	P54841

Expressionⁱ

Tissue specificityⁱ

Expressed in pancreatic alpha-cells (glucagon-positive cells), in podocytes of the kidney and macrophages (at protein level). Most abundant in kidney, gut, lung and brain.3 Publications

Developmental stageⁱ

Expressed in pancreatic alpha-cells at 10.5 dpc. Expressed in insulin and glucagon islet progenitor cells at 12 dpc onwards (at protein level). Detectable at 8.0 dpc (one somite) as a band in the caudal hindbrain and by 8.5 dpc (six to eight somites) as a sharp rostral edge coincident with the rhombomeres (r) 4 and 5 boundary and a diffuse caudal edge located midway through r6. Expressed in the lens epithelial cells at 10.5 to 14.5 dpc.3 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000074622 Expressed in 282 organ(s), highest expression level in molar tooth
CleanExⁱ	MM_MAF1
Genevisibleⁱ	P54841 MM

Interactionⁱ

Subunit structureⁱ

Homodimer or heterodimer with other bHLH-Zip transcription factors. Forms homodimers and heterodimers with FOS, FOSB and FOSL2, but not with JUN proteins (JUN, JUNB and JUND). Interacts with the intracellular cytoplasmic domain of LRP1 (LRPICD); the interaction results in a moderate reduction of MAFB transcriptional potential (By similarity). Binds DNA as a homodimer or a heterodimer. Interacts with PAX6; the interaction is direct. Interacts with ETS1 and LRP1.By similarity3 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Fos	P01101	4	EBI-16093217,EBI-4288185

With

Entry

#Exp.

IntAct

Notes

Fos

P01101

4

EBI-16093217,EBI-4288185

GO - Molecular functionⁱ

protein heterodimerization activity Source: MGI
protein homodimerization activity Source: MGI
transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201014, 5 interactors
Database of interacting proteins More...DIPⁱ	DIP-60663N
IntActⁱ	P54841, 1 interactor
STRINGⁱ	10090.ENSMUSP00000096728

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	215 – 218	Combined sources	4
Helixⁱ	221 – 225	Combined sources	5
Turnⁱ	228 – 230	Combined sources	3
Helixⁱ	233 – 299	Combined sources	67

Show more details

3D structure databases

ProteinModelPortalⁱ	P54841
SMRⁱ	P54841
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	238 – 301	bZIPPROSITE-ProRule annotationAdd BLAST		64

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	238 – 263	Basic motifPROSITE-ProRule annotationAdd BLAST		26
Regionⁱ	266 – 287	Leucine-zipperPROSITE-ProRule annotationAdd BLAST		22

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	131 – 143	Poly-HisAdd BLAST		13
Compositional biasⁱ	158 – 167	Poly-His		10

Domainⁱ

The leucine-zipper domain is involved in the interaction with LRPICD.

Sequence similaritiesⁱ

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG4196 Eukaryota ENOG41102C7 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00550000074549
HOGENOMⁱ	HOG000261683
HOVERGENⁱ	HBG000313
InParanoidⁱ	P54841
KEGG Orthology (KO) More...KOⁱ	K09036
OMAⁱ	CNRLQPQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	P54841
TreeFamⁱ	TF325689

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR013592 Maf_TF_N IPR028571 MafB IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF10 PTHR10129:SF10, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit PF08383 Maf_N, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P54841-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAAELSMGQE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ 
        60         70         80         90        100
PAGSVSSTPL STPCSSVPSS PSFSPTEPKT HLEDLYWMAS NYQQMNPEAL 
       110        120        130        140        150
NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV 
       160        170        180        190        200
THDDLGQHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHATAAATA 
       210        220        230        240        250
AGGNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG 
       260        270        280        290        300
YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK 
       310        320 
LANSGFREAG STSDSPSSPE FFL

Length:323

Mass (Da):35,809

Last modified:October 1, 1996 - v1

Checksum:ⁱD77AE07ABD9C2AD2

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	275	Q → K in BAE32860 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L36435 mRNA Translation: AAA65689.1 AF180338 Genomic DNA Translation: AAD56221.1 AK132425 mRNA Translation: BAE21161.1 AK143209 mRNA Translation: BAE25306.1 AK154830 mRNA Translation: BAE32860.1 AL591665 Genomic DNA Translation: CAM21248.1 CH466551 Genomic DNA Translation: EDL06288.1 BC016434 mRNA Translation: AAH16434.1 BC038256 mRNA Translation: AAH38256.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16994.1
PIRⁱ	I49529
NCBI Reference Sequences More...RefSeqⁱ	NP_034788.1, NM_010658.3
UniGeneⁱ	Mm.330745

Genome annotation databases

Ensemblⁱ	ENSMUST00000099126; ENSMUSP00000096728; ENSMUSG00000074622
GeneIDⁱ	16658
KEGGⁱ	mmu:16658
UCSC genome browser More...UCSCⁱ	uc008nqw.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P54841	Uncharacterized protein (Fragment)		MOUSE		168	UniRef100_P54841

Protein	Similar proteins		Species	Score	Length	Source
P54841	Transcription factor MafB		HUMAN		323	UniRef90_P54841
Transcription factor MafB		RAT		323
Transcription factor MafB		MACFA		323
Uncharacterized protein (Fragment)		MOUSE		168
MAF bZIP transcription factor B		CERAT		342
+85

Protein	Similar proteins		Species	Score	Length	Source
P54841	Transcription factor MafB		RAT		323	UniRef50_P54841
Transcription factor MafB		MACFA		323
Transcription factor MafB		HUMAN		323
Transcription factor MafB		XENTR		316
Transcription factor MafB		XENLA		313
+190

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L36435 mRNA Translation: AAA65689.1 AF180338 Genomic DNA Translation: AAD56221.1 AK132425 mRNA Translation: BAE21161.1 AK143209 mRNA Translation: BAE25306.1 AK154830 mRNA Translation: BAE32860.1 AL591665 Genomic DNA Translation: CAM21248.1 CH466551 Genomic DNA Translation: EDL06288.1 BC016434 mRNA Translation: AAH16434.1 BC038256 mRNA Translation: AAH38256.1
CCDSⁱ	CCDS16994.1
PIRⁱ	I49529
RefSeqⁱ	NP_034788.1, NM_010658.3
UniGeneⁱ	Mm.330745

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2WT7	X-ray	2.30	B	214-303	[»]
	2WTY	X-ray	2.90	A/B	211-306	[»]
	4AUW	X-ray	2.90	A/B/E/F	211-305	[»]
ProteinModelPortalⁱ	P54841
SMRⁱ	P54841
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201014, 5 interactors
DIPⁱ	DIP-60663N
IntActⁱ	P54841, 1 interactor
STRINGⁱ	10090.ENSMUSP00000096728

PTM databases

iPTMnetⁱ	P54841
PhosphoSitePlusⁱ	P54841

Proteomic databases

MaxQBⁱ	P54841
PaxDbⁱ	P54841
PeptideAtlasⁱ	P54841
PRIDEⁱ	P54841

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000099126; ENSMUSP00000096728; ENSMUSG00000074622
GeneIDⁱ	16658
KEGGⁱ	mmu:16658
UCSCⁱ	uc008nqw.2 mouse

Organism-specific databases

CTDⁱ	9935
MGIⁱ	MGI:104555 Mafb

Phylogenomic databases

eggNOGⁱ	KOG4196 Eukaryota ENOG41102C7 LUCA
GeneTreeⁱ	ENSGT00550000074549
HOGENOMⁱ	HOG000261683
HOVERGENⁱ	HBG000313
InParanoidⁱ	P54841
KOⁱ	K09036
OMAⁱ	CNRLQPQ
OrthoDBⁱ	EOG091G0H46
PhylomeDBⁱ	P54841
TreeFamⁱ	TF325689

Miscellaneous databases

ChiTaRSⁱ	Mafb mouse
Protein Ontology More...PROⁱ	PR:P54841
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000074622 Expressed in 282 organ(s), highest expression level in molar tooth
CleanExⁱ	MM_MAF1
Genevisibleⁱ	P54841 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR004827 bZIP IPR004826 bZIP_Maf IPR013592 Maf_TF_N IPR028571 MafB IPR008917 TF_DNA-bd_sf IPR024874 Transciption_factor_Maf_fam
PANTHERⁱ	PTHR10129 PTHR10129, 1 hit PTHR10129:SF10 PTHR10129:SF10, 1 hit
Pfamⁱ	View protein in Pfam PF03131 bZIP_Maf, 1 hit PF08383 Maf_N, 1 hit
SMARTⁱ	View protein in SMART SM00338 BRLZ, 1 hit
SUPFAMⁱ	SSF47454 SSF47454, 1 hit
PROSITEⁱ	View protein in PROSITE PS50217 BZIP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MAFB_MOUSE
Accessionⁱ	P54841Primary (citable) accession number: P54841 Secondary accession number(s): Q3U3C8, Q3UPT9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	October 1, 1996
	Last modified:	September 12, 2018
	This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P54841 (MAFB_MOUSE)

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Transcription factor MafB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ