UniProtKB - P54841 (MAFB_MOUSE)
Protein
Transcription factor MafB
Gene
Mafb
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, osteoclast, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Required for the transcriptional activation of HOXB3 in the rhombomere r5 in the hindbrain (PubMed:11823429).By similarity9 Publications
GO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- identical protein binding Source: MGI
- protein-containing complex binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- abducens nerve formation Source: UniProtKB
- brain segmentation Source: MGI
- inner ear morphogenesis Source: MGI
- negative regulation of erythrocyte differentiation Source: MGI
- negative regulation of osteoclast differentiation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of myeloid cell differentiation Source: GO_Central
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: MGI
- respiratory gaseous exchange by respiratory system Source: MGI
- rhombomere 5 development Source: MGI
- rhombomere 6 development Source: MGI
- segment specification Source: MGI
- T cell differentiation in thymus Source: MGI
- thymus development Source: MGI
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor MafBShort name: Maf-B Alternative name(s): Kreisler Segmentation protein Kr Transcription factor Maf-1 V-maf musculoaponeurotic fibrosarcoma oncogene homolog B |
Gene namesi | Name:Mafb Synonyms:Krml, Maf1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104555, Mafb |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation1 Publication
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Other locations
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mice show a defect in frequency of respiratory rhythm with a fatal apnea at birth due to lack of neurons from the preBoetC region. They displayed renal dysgenesis with abnormal podocyte differentiation as well as tubular apoptosis. They show altered actin-dependent macrophage morphology. They show a reduced number of cells expressing insulin and glucagon. Embryos also lack the abducens nerve which normaly innervates the lateral rectus muscle that is involved in eye movement (PubMed:27181683).6 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 32 | K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-297. 1 Publication | 1 | |
Mutagenesisi | 248 | N → A: Reduces ability to activate insulin and glucagon gene expression. 1 Publication | 1 | |
Mutagenesisi | 248 | N → S: Loss of transcriptional activity. 1 Publication | 1 | |
Mutagenesisi | 297 | K → R: Loss of SUMO modification, increases transactivation activity, increases macrophage differentiation and inhibits myeloid progenitor growth; when associated with R-32. 1 Publication | 1 |
Keywords - Diseasei
Proto-oncogene, Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076495 | 1 – 323 | Transcription factor MafBAdd BLAST | 323 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 32 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication | ||
Cross-linki | 297 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication |
Post-translational modificationi
Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.1 Publication
Keywords - PTMi
Isopeptide bond, Ubl conjugationProteomic databases
MaxQBi | P54841 |
PaxDbi | P54841 |
PeptideAtlasi | P54841 |
PRIDEi | P54841 |
ProteomicsDBi | 287292 |
PTM databases
iPTMneti | P54841 |
PhosphoSitePlusi | P54841 |
Expressioni
Tissue specificityi
Expressed in pancreatic alpha-cells (glucagon-positive cells), in podocytes of the kidney and macrophages (at protein level). Most abundant in kidney, gut, lung and brain.3 Publications
Developmental stagei
Expressed in pancreatic alpha-cells at 10.5 dpc. Expressed in insulin and glucagon islet progenitor cells at 12 dpc onwards (at protein level). Detectable at 8.0 dpc (one somite) as a band in the caudal hindbrain and by 8.5 dpc (six to eight somites) as a sharp rostral edge coincident with the rhombomeres (r) 4 and 5 boundary and a diffuse caudal edge located midway through r6. Expressed in the lens epithelial cells at 10.5 to 14.5 dpc.3 Publications
Gene expression databases
Bgeei | ENSMUSG00000074622, Expressed in molar tooth and 298 other tissues |
Genevisiblei | P54841, MM |
Interactioni
Subunit structurei
Homodimer or heterodimer with other bHLH-Zip transcription factors. Forms homodimers and heterodimers with FOS, FOSB and FOSL2, but not with JUN proteins (JUN, JUNB and JUND).
Interacts with the intracellular cytoplasmic domain of LRP1 (LRPICD); the interaction results in a moderate reduction of MAFB transcriptional potential (By similarity). Binds DNA as a homodimer or a heterodimer.
Interacts with PAX6; the interaction is direct.
Interacts with ETS1 and LRP1.
By similarity3 PublicationsBinary interactionsi
P54841
With | #Exp. | IntAct |
---|---|---|
Fos [P01101] | 4 | EBI-16093217,EBI-4288185 |
GO - Molecular functioni
- identical protein binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201014, 5 interactors |
DIPi | DIP-60663N |
IntActi | P54841, 1 interactor |
STRINGi | 10090.ENSMUSP00000096728 |
Miscellaneous databases
RNActi | P54841, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P54841 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 238 – 301 | bZIPPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 238 – 263 | Basic motifPROSITE-ProRule annotationAdd BLAST | 26 | |
Regioni | 266 – 287 | Leucine-zipperPROSITE-ProRule annotationAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 131 – 143 | Poly-HisAdd BLAST | 13 | |
Compositional biasi | 158 – 167 | Poly-His | 10 |
Domaini
The leucine-zipper domain is involved in the interaction with LRPICD.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160486 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54841 |
OMAi | PTEQKHH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54841 |
TreeFami | TF325689 |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR013592, Maf_TF_N IPR028571, MafB IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF10, PTHR10129:SF10, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
i Sequence
Sequence statusi: Complete.
P54841-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAELSMGQE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ
60 70 80 90 100
PAGSVSSTPL STPCSSVPSS PSFSPTEPKT HLEDLYWMAS NYQQMNPEAL
110 120 130 140 150
NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV
160 170 180 190 200
THDDLGQHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHATAAATA
210 220 230 240 250
AGGNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG
260 270 280 290 300
YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK
310 320
LANSGFREAG STSDSPSSPE FFL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 275 | Q → K in BAE32860 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36435 mRNA Translation: AAA65689.1 AF180338 Genomic DNA Translation: AAD56221.1 AK132425 mRNA Translation: BAE21161.1 AK143209 mRNA Translation: BAE25306.1 AK154830 mRNA Translation: BAE32860.1 AL591665 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06288.1 BC016434 mRNA Translation: AAH16434.1 BC038256 mRNA Translation: AAH38256.1 |
CCDSi | CCDS16994.1 |
PIRi | I49529 |
RefSeqi | NP_034788.1, NM_010658.3 |
Genome annotation databases
Ensembli | ENSMUST00000099126; ENSMUSP00000096728; ENSMUSG00000074622 |
GeneIDi | 16658 |
KEGGi | mmu:16658 |
UCSCi | uc008nqw.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L36435 mRNA Translation: AAA65689.1 AF180338 Genomic DNA Translation: AAD56221.1 AK132425 mRNA Translation: BAE21161.1 AK143209 mRNA Translation: BAE25306.1 AK154830 mRNA Translation: BAE32860.1 AL591665 Genomic DNA No translation available. CH466551 Genomic DNA Translation: EDL06288.1 BC016434 mRNA Translation: AAH16434.1 BC038256 mRNA Translation: AAH38256.1 |
CCDSi | CCDS16994.1 |
PIRi | I49529 |
RefSeqi | NP_034788.1, NM_010658.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2WT7 | X-ray | 2.30 | B | 214-303 | [»] | |
2WTY | X-ray | 2.90 | A/B | 211-306 | [»] | |
4AUW | X-ray | 2.90 | A/B/E/F | 211-305 | [»] | |
SMRi | P54841 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201014, 5 interactors |
DIPi | DIP-60663N |
IntActi | P54841, 1 interactor |
STRINGi | 10090.ENSMUSP00000096728 |
PTM databases
iPTMneti | P54841 |
PhosphoSitePlusi | P54841 |
Proteomic databases
MaxQBi | P54841 |
PaxDbi | P54841 |
PeptideAtlasi | P54841 |
PRIDEi | P54841 |
ProteomicsDBi | 287292 |
Protocols and materials databases
Antibodypediai | 956, 290 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000099126; ENSMUSP00000096728; ENSMUSG00000074622 |
GeneIDi | 16658 |
KEGGi | mmu:16658 |
UCSCi | uc008nqw.2, mouse |
Organism-specific databases
CTDi | 9935 |
MGIi | MGI:104555, Mafb |
Phylogenomic databases
eggNOGi | KOG4196, Eukaryota |
GeneTreei | ENSGT00940000160486 |
HOGENOMi | CLU_063062_0_0_1 |
InParanoidi | P54841 |
OMAi | PTEQKHH |
OrthoDBi | 1395389at2759 |
PhylomeDBi | P54841 |
TreeFami | TF325689 |
Miscellaneous databases
BioGRID-ORCSi | 16658, 2 hits in 52 CRISPR screens |
ChiTaRSi | Mafb, mouse |
PROi | PR:P54841 |
RNActi | P54841, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000074622, Expressed in molar tooth and 298 other tissues |
Genevisiblei | P54841, MM |
Family and domain databases
InterProi | View protein in InterPro IPR004827, bZIP IPR004826, bZIP_Maf IPR013592, Maf_TF_N IPR028571, MafB IPR008917, TF_DNA-bd_sf IPR024874, Transcription_factor_Maf_fam |
PANTHERi | PTHR10129, PTHR10129, 1 hit PTHR10129:SF10, PTHR10129:SF10, 1 hit |
Pfami | View protein in Pfam PF03131, bZIP_Maf, 1 hit PF08383, Maf_N, 1 hit |
SMARTi | View protein in SMART SM00338, BRLZ, 1 hit |
SUPFAMi | SSF47454, SSF47454, 1 hit |
PROSITEi | View protein in PROSITE PS50217, BZIP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAFB_MOUSE | |
Accessioni | P54841Primary (citable) accession number: P54841 Secondary accession number(s): Q3U3C8, Q3UPT9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families