UniProtKB - P54830 (PTN5_MOUSE)
Tyrosine-protein phosphatase non-receptor type 5
Ptpn5
Functioni
Catalytic activityi
- EC:3.1.3.48PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 437 | SubstrateBy similarity | 1 | |
Active sitei | 472 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 | |
Binding sitei | 516 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- glutamate receptor binding Source: MGI
- identical protein binding Source: MGI
- mitogen-activated protein kinase binding Source: MGI
- phosphotyrosine residue binding Source: MGI
- protein kinase binding Source: MGI
- protein tyrosine phosphatase activity Source: MGI
GO - Biological processi
- exploration behavior Source: MGI
- negative regulation of MAP kinase activity Source: MGI
- peptidyl-tyrosine dephosphorylation Source: MGI
- positive regulation of long-term synaptic potentiation Source: MGI
- positive regulation of neuron death Source: MGI
- positive regulation of neuron projection development Source: MGI
- positive regulation of protein import into nucleus Source: MGI
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of protein targeting to mitochondrion Source: MGI
- positive regulation of receptor internalization Source: MGI
- positive regulation of response to drug Source: MGI
- protein dephosphorylation Source: MGI
- response to immobilization stress Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein phosphatase non-receptor type 5 (EC:3.1.3.48)Alternative name(s): Neural-specific protein-tyrosine phosphatase Striatum-enriched protein-tyrosine phosphatase Short name: STEP |
Gene namesi | Name:Ptpn5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97807, Ptpn5 |
Subcellular locationi
Endoplasmic reticulum
Other locations
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Plasma Membrane
- synaptic membrane Source: MGI
Other locations
- axon Source: MGI
- integral component of membrane Source: UniProtKB-KW
- membrane Source: MGI
- neuronal cell body Source: MGI
- perikaryon Source: MGI
- proximal dendrite Source: MGI
- synaptic vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 64 – 84 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094756 | 1 – 541 | Tyrosine-protein phosphatase non-receptor type 5Add BLAST | 541 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 221 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 231 | Phosphothreonine; by MAPKBy similarity | 1 | |
Modified residuei | 244 | Phosphoserine; by MAPKBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P54830 |
PaxDbi | P54830 |
PeptideAtlasi | P54830 |
PRIDEi | P54830 |
PTM databases
iPTMneti | P54830 |
PhosphoSitePlusi | P54830 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000030854, Expressed in caudate-putamen and 189 other tissues |
Genevisiblei | P54830, MM |
Interactioni
Binary interactionsi
Isoform STEP61 [P54830-1]
With | #Exp. | IntAct |
---|---|---|
MAPK14 - isoform CSBP2 [Q16539-1] from Homo sapiens. | 6 | EBI-16067443,EBI-15834191 |
GO - Molecular functioni
- glutamate receptor binding Source: MGI
- identical protein binding Source: MGI
- mitogen-activated protein kinase binding Source: MGI
- phosphotyrosine residue binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202488, 12 interactors |
DIPi | DIP-32453N |
ELMi | P54830 |
IntActi | P54830, 4 interactors |
MINTi | P54830 |
STRINGi | 10090.ENSMUSP00000033142 |
Miscellaneous databases
RNActi | P54830, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P54830 |
SMRi | P54830 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 276 – 531 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 256 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 472 – 478 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0789, Eukaryota |
GeneTreei | ENSGT00940000159916 |
HOGENOMi | CLU_001645_10_2_1 |
InParanoidi | P54830 |
OMAi | YIMSPRE |
OrthoDBi | 411281at2759 |
TreeFami | TF331016 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR008356, Tyr_Pase_KIM-con IPR016334, Tyr_Pase_rcpt_R/non-rcpt_5 IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR46198, PTHR46198, 1 hit |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 1 hit |
PIRSFi | PIRSF001997, PTPRR, 1 hit |
PRINTSi | PR01778, KIMPTPASE PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCCSERLLGL PQPVEMEAPD EAEGLPSKQK EMPPPPPPSP PSEPAQKLPP
60 70 80 90 100
QGAGSHSLTV RSSLCLFAAS QFLLACGVLW LSGHGHSWLQ NTTDLISSSL
110 120 130 140 150
TVLNHLGPVA WLGSGTWGIP SLLLVSLTVS LVIVTTLVWH LLKAPPEPPA
160 170 180 190 200
PLPPEDRRQS VSRQPSFTYS EWMEEKVEDD FLDLDAVPET PVFDCVMDIK
210 220 230 240 250
PETDPASLTV KSMGLQERRG SNVSLTLDMC TPGCNEEGFG YLVSPREESA
260 270 280 290 300
HEYLLSASRV LRAEELHEKA LDPFLLQAEF FEIPMNFVDP KEYDIPGLVR
310 320 330 340 350
KNRYKTILPN PHSRVRLTSP DPEDPLSSYI NANYIRGYSG EEKVYIATQG
360 370 380 390 400
PIVSTVADFW RMVWQERTPI IVMITNIEEM NEKCTEYWPE EQVVHDGVEI
410 420 430 440 450
TVQKVIHTED YRLRLISLRR GTEERSLKHY WFTSWPDQKT PDRAPPLLHL
460 470 480 490 500
VREVEEAAQQ EGPHCSPIIV HCSAGIGRTG CFIATSICCQ QLRREGVVDI
510 520 530 540
LKTTCQLRQD RGGMIQTCEQ YQFVHHAMSL YEKQLSLQSS E
The sequence of this isoform differs from the canonical sequence as follows:
1-172: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
337-346: GYSGEEKVYI → VCSSIPRAFH
347-541: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-172: Missing.
337-346: GYSGEEKVYI → VCSSIPRAFH
347-541: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 426 | S → T in AAA73574 (PubMed:7494467).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_005126 | 1 – 172 | Missing in isoform STEP20 and isoform STEP46. 1 PublicationAdd BLAST | 172 | |
Alternative sequenceiVSP_005127 | 337 – 346 | GYSGEEKVYI → VCSSIPRAFH in isoform STEP38 and isoform STEP20. 1 Publication | 10 | |
Alternative sequenceiVSP_005128 | 347 – 541 | Missing in isoform STEP38 and isoform STEP20. 1 PublicationAdd BLAST | 195 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28217 mRNA Translation: AAA73574.1 U28216 mRNA Translation: AAA73573.1 S80329 mRNA Translation: AAB35656.2 AK038416 mRNA Translation: BAC29993.1 CH466603 Genomic DNA Translation: EDL22958.1 BC079592 mRNA Translation: AAH79592.1 |
CCDSi | CCDS21294.1 [P54830-1] |
RefSeqi | NP_001157037.1, NM_001163565.1 [P54830-1] NP_038671.2, NM_013643.2 [P54830-1] XP_006540776.1, XM_006540713.3 [P54830-1] XP_006540777.1, XM_006540714.3 [P54830-1] XP_006540778.1, XM_006540715.3 [P54830-1] XP_017177535.1, XM_017322046.1 [P54830-1] |
Genome annotation databases
Ensembli | ENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854 [P54830-1] ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854 [P54830-1] |
GeneIDi | 19259 |
KEGGi | mmu:19259 |
UCSCi | uc009gzz.2, mouse [P54830-1] uc009hac.1, mouse [P54830-3] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U28217 mRNA Translation: AAA73574.1 U28216 mRNA Translation: AAA73573.1 S80329 mRNA Translation: AAB35656.2 AK038416 mRNA Translation: BAC29993.1 CH466603 Genomic DNA Translation: EDL22958.1 BC079592 mRNA Translation: AAH79592.1 |
CCDSi | CCDS21294.1 [P54830-1] |
RefSeqi | NP_001157037.1, NM_001163565.1 [P54830-1] NP_038671.2, NM_013643.2 [P54830-1] XP_006540776.1, XM_006540713.3 [P54830-1] XP_006540777.1, XM_006540714.3 [P54830-1] XP_006540778.1, XM_006540715.3 [P54830-1] XP_017177535.1, XM_017322046.1 [P54830-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6H8S | X-ray | 1.77 | A | 244-539 | [»] | |
BMRBi | P54830 | |||||
SMRi | P54830 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202488, 12 interactors |
DIPi | DIP-32453N |
ELMi | P54830 |
IntActi | P54830, 4 interactors |
MINTi | P54830 |
STRINGi | 10090.ENSMUSP00000033142 |
PTM databases
iPTMneti | P54830 |
PhosphoSitePlusi | P54830 |
Proteomic databases
MaxQBi | P54830 |
PaxDbi | P54830 |
PeptideAtlasi | P54830 |
PRIDEi | P54830 |
Protocols and materials databases
Antibodypediai | 25176, 286 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000033142; ENSMUSP00000033142; ENSMUSG00000030854 [P54830-1] ENSMUST00000102626; ENSMUSP00000099686; ENSMUSG00000030854 [P54830-1] |
GeneIDi | 19259 |
KEGGi | mmu:19259 |
UCSCi | uc009gzz.2, mouse [P54830-1] uc009hac.1, mouse [P54830-3] |
Organism-specific databases
CTDi | 84867 |
MGIi | MGI:97807, Ptpn5 |
Phylogenomic databases
eggNOGi | KOG0789, Eukaryota |
GeneTreei | ENSGT00940000159916 |
HOGENOMi | CLU_001645_10_2_1 |
InParanoidi | P54830 |
OMAi | YIMSPRE |
OrthoDBi | 411281at2759 |
TreeFami | TF331016 |
Miscellaneous databases
BioGRID-ORCSi | 19259, 4 hits in 19 CRISPR screens |
ChiTaRSi | Ptpn5, mouse |
PROi | PR:P54830 |
RNActi | P54830, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030854, Expressed in caudate-putamen and 189 other tissues |
Genevisiblei | P54830, MM |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR008356, Tyr_Pase_KIM-con IPR016334, Tyr_Pase_rcpt_R/non-rcpt_5 IPR000387, TYR_PHOSPHATASE_dom |
PANTHERi | PTHR46198, PTHR46198, 1 hit |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 1 hit |
PIRSFi | PIRSF001997, PTPRR, 1 hit |
PRINTSi | PR01778, KIMPTPASE PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTN5_MOUSE | |
Accessioni | P54830Primary (citable) accession number: P54830 Secondary accession number(s): Q64694, Q8CAN0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | February 10, 2009 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families