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Protein

ATP-dependent RNA helicase ddx6

Gene

ddx6

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase that is an integral component of messenger ribonucleoprotein complexes (mRNPs), storage particles that mask maternal mRNAs from the translational apparatus during oocyte maturation.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 146ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Helicase, Hydrolase, Ribonucleoprotein, RNA-binding
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase ddx6 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase p54
Short name:
P54H
Short name:
Xp54
DEAD box protein 6
Gene namesi
Name:ddx6
Synonyms:p54h
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-922237 ddx6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549851 – 481ATP-dependent RNA helicase ddx6Add BLAST481

Proteomic databases

PRIDEiP54824

Expressioni

Tissue specificityi

In adults, expression is restricted to the germline cells of the ovary.1 Publication

Developmental stagei

Expressed maternally. Expression peaks at stage I of oogenesis and remains fairly constant through to the end of oogenesis and, after fertilization, up to blastula. From blastula, through gastrula and neurula, expression declines significantly. May also be expressed zygotically since a low level of expression is detected up to the free-feeding tadpole stage (embryonic stage 42) (at protein level).2 Publications

Interactioni

Subunit structurei

Component of a ribonucleoprotein (RNP) complex, composed at least of cpeb1, lsm14b/rap55b, ddx6/Xp54, ybx2/frgy2, pat1/P100, eif4enif1/4E-T and eif4e1b. Component of a ribonucleoprotein (RNP) complex, composed at least of elavl1/elrA and/or elavl2/elrB, igf2bp3/vg1RBP, ddx6/Xp54, ybx2/frgy2, lsm14b/rap55b and, in a subset of RNP complexes, stau1/staufen. Component of a ribonucleoprotein (RNP) complex, composed at least of lsm14a/rap55a, ybx2/frgy2, ddx6/Xp54 and eif4enif1/4E-T. Interacts with lsm14a/rap55a.4 Publications

Protein-protein interaction databases

BioGridi100406, 1 interactor
IntActiP54824, 3 interactors

Structurei

3D structure databases

SMRiP54824
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 297Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST172
Domaini307 – 467Helicase C-terminalPROSITE-ProRule annotationAdd BLAST161

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 123Q motifAdd BLAST29
Motifi245 – 248DEAD box4

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG106685
KOiK12614

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P54824-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTARTENPV LMGMSSQNGQ LRGPLKPSAG PGGGGTQTQQ INQLKNASTI
60 70 80 90 100
NSGSQQQAQS MSSIIKPGDD WKKTLKLPPK DLRIKTSDVT STKGNEFEDY
110 120 130 140 150
CLKRELLMGI FEMGWEKPSP IQEESIPIAL SGRDILARAK NGTGKSGAYL
160 170 180 190 200
IPLLERLDLK KDCIQAMVIV PTRELALQVS QICIQVSKHM GGAKVMATTG
210 220 230 240 250
GTNLRDDIMR LDDTVHVVIA TPGRILDLIK KGVAKVDHIQ MIVLDEADKL
260 270 280 290 300
LSQDFMQIME DIIMTLPKNR QILLYSATFP LSVQKFMTLH LQKPYEINLM
310 320 330 340 350
EELTLKGVTQ YYAYVTERQK VHCLNTLFSR LQINQSIIFC NSSQRVELLA
360 370 380 390 400
KKISQLGYSC FYIHAKMRQE HRNRVFHDFR NGLCRNLVCT DLFTRGIDIQ
410 420 430 440 450
AVNVVINFDF PKLAETYLHR IGRSGRFGHL GLAINLITYD DRFNLKSIEE
460 470 480
QLGTEIKPIP SSIDKNLYVA EYHSESGEDK P
Length:481
Mass (Da):54,074
Last modified:February 9, 2010 - v2
Checksum:i3CB5516F707C7C3D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146S → T in CAA63149 (PubMed:9023105).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92421 mRNA Translation: CAA63149.1
BC170228 mRNA Translation: AAI70228.1
BC170230 mRNA Translation: AAI70230.1
RefSeqiNP_001083721.1, NM_001090252.1
XP_018079874.1, XM_018224385.1
XP_018079875.1, XM_018224386.1
XP_018079876.1, XM_018224387.1
XP_018079877.1, XM_018224388.1
XP_018079879.1, XM_018224390.1
UniGeneiXl.157

Genome annotation databases

GeneIDi399080
KEGGixla:399080

Similar proteinsi

Entry informationi

Entry nameiDDX6_XENLA
AccessioniPrimary (citable) accession number: P54824
Secondary accession number(s): B7ZRN4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: February 9, 2010
Last modified: May 23, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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