UniProtKB - P54823 (DDX6_MOUSE)
Probable ATP-dependent RNA helicase DDX6
Ddx6
Functioni
Catalytic activityi
- EC:3.6.4.13By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 140 – 147 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- mRNA binding Source: GO_Central
- protein domain specific binding Source: MGI
- RNA helicase activity Source: UniProtKB-EC
GO - Biological processi
- negative regulation of neuron differentiation Source: MGI
- negative regulation of translation Source: UniProtKB
- P-body assembly Source: UniProtKB
- positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
- spermatid differentiation Source: Ensembl
- stem cell population maintenance Source: MGI
- stress granule assembly Source: GO_Central
- viral RNA genome packaging Source: MGI
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-430039, mRNA decay by 5' to 3' exoribonuclease |
Names & Taxonomyi
Protein namesi | Recommended name: Probable ATP-dependent RNA helicase DDX6Curated (EC:3.6.4.13Curated)Alternative name(s): ATP-dependent RNA helicase p54 DEAD box protein 6 Oncogene RCK homolog |
Gene namesi | Name:Ddx6 Synonyms:Hlr2, Rck |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104976, Ddx6 |
Subcellular locationi
Cytoskeleton
- outer dense fiber Source: MGI
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
Other locations
- adherens junction Source: MGI
- chromatoid body Source: MGI
- concave side of sperm head Source: MGI
- cytoplasm Source: MGI
- cytoplasmic ribonucleoprotein granule Source: MGI
- cytoplasmic stress granule Source: UniProtKB
- heterochromatin Source: MGI
- P-body Source: MGI
- perinuclear region of cytoplasm Source: MGI
- RISC complex Source: MGI
- sperm annulus Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000054984 | 1 – 483 | Probable ATP-dependent RNA helicase DDX6Add BLAST | 483 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P54823 |
jPOSTi | P54823 |
MaxQBi | P54823 |
PaxDbi | P54823 |
PeptideAtlasi | P54823 |
PRIDEi | P54823 |
PTM databases
iPTMneti | P54823 |
PhosphoSitePlusi | P54823 |
SwissPalmi | P54823 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000032097, Expressed in thymus and 305 other tissues |
Genevisiblei | P54823, MM |
Interactioni
Subunit structurei
Interacts with LSM14A, LSM14B, EIF4ENIF1/4E-T, PATL1, EDC3 and EDC4 (By similarity).
Forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS (By similarity).
Interacts with LIMD1, WTIP and AJUBA (By similarity).
Interacts with APOBEC3G in an RNA-dependent manner (By similarity).
Interacts with RC3H1 (PubMed:20639877).
Interacts with ATXN2L (By similarity).
Interacts with MCRIP1 (By similarity).
Interacts with MCRIP2 (By similarity).
Interacts with NUFIP2 (By similarity).
Interacts with TRIM71 (via NHL repeats) in an RNA-dependent manner (By similarity).
By similarity1 PublicationGO - Molecular functioni
- protein domain specific binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199087, 44 interactors |
IntActi | P54823, 8 interactors |
MINTi | P54823 |
STRINGi | 10090.ENSMUSP00000128421 |
Miscellaneous databases
RNActi | P54823, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 127 – 298 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 172 | |
Domaini | 308 – 468 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 161 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 96 – 124 | Q motifAdd BLAST | 29 | |
Motifi | 246 – 249 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0326, Eukaryota |
GeneTreei | ENSGT00900000141067 |
HOGENOMi | CLU_003041_30_0_1 |
InParanoidi | P54823 |
OMAi | DWNLMSS |
OrthoDBi | 583315at2759 |
PhylomeDBi | P54823 |
TreeFami | TF300440 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSTARTENPV IMGLSSQNGQ LRGPVKASAG PGGGGTQPQP QLNQLKNTST
60 70 80 90 100
INNGTPQQAQ SMAATIKPGD DWKKTLKLPP KDLRIKTSDV TSTKGNEFED
110 120 130 140 150
YCLKRELLMG IFEMGWEKPS PIQEESIPIA LSGRDILARA KNGTGKSGAY
160 170 180 190 200
LIPLLERLDL KKDNIQAMVI VPTRELALQV SQICIQVSKH MGGAKVMATT
210 220 230 240 250
GGTNLRDDIM RLDDTVHVVI ATPGRILDLI KKGVAKVDHV QMIVLDEADK
260 270 280 290 300
LLSQDFVQIM EDIILTLPKN RQILLYSATF PLSVQKFMNS HLQKPYEINL
310 320 330 340 350
MEELTLKGVT QYYAYVTERQ KVHCLNTLFS RLQINQSIIF CNSSQRVELL
360 370 380 390 400
AKKISQLGYS CFYIHAKMRQ EHRNRVFHDF RNGLCRNLVC TDLFTRGIDI
410 420 430 440 450
QAVNVVINFD FPKLAETYLH RIGRSGRFGH LGLAINLITY DDRFNLKSIE
460 470 480
EQLGTEIKPI PSNIDKSLYV AEYHSEPAED EKP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 – 42 | PQPQL → TQQQM in AAB94769 (PubMed:9883581).Curated | 5 | |
Sequence conflicti | 202 | G → P in AAB94769 (PubMed:9883581).Curated | 1 | |
Sequence conflicti | 241 | Q → R in BAC35670 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 311 | Q → E in BAC35670 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 381 | R → E in AAB94769 (PubMed:9883581).Curated | 1 | |
Sequence conflicti | 407 | I → M in BAC35670 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 422 | I → V in AAB94769 (PubMed:9883581).Curated | 1 | |
Sequence conflicti | 478 | A → V in AAB94769 (PubMed:9883581).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50494 mRNA Translation: BAA09088.1 AK054144 mRNA Translation: BAC35670.1 AK148483 mRNA Translation: BAE28578.1 BC021452 mRNA Translation: AAH21452.1 AF038995 mRNA Translation: AAB94769.1 |
CCDSi | CCDS23116.1 |
RefSeqi | NP_001104296.1, NM_001110826.1 NP_031867.1, NM_007841.4 NP_851841.2, NM_181324.3 XP_011240693.1, XM_011242391.2 XP_011240694.1, XM_011242392.2 XP_017168613.1, XM_017313124.1 |
Genome annotation databases
Ensembli | ENSMUST00000170489; ENSMUSP00000128421; ENSMUSG00000032097 ENSMUST00000217034; ENSMUSP00000149620; ENSMUSG00000032097 |
GeneIDi | 13209 |
KEGGi | mmu:13209 |
UCSCi | uc009pdy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D50494 mRNA Translation: BAA09088.1 AK054144 mRNA Translation: BAC35670.1 AK148483 mRNA Translation: BAE28578.1 BC021452 mRNA Translation: AAH21452.1 AF038995 mRNA Translation: AAB94769.1 |
CCDSi | CCDS23116.1 |
RefSeqi | NP_001104296.1, NM_001110826.1 NP_031867.1, NM_007841.4 NP_851841.2, NM_181324.3 XP_011240693.1, XM_011242391.2 XP_011240694.1, XM_011242392.2 XP_017168613.1, XM_017313124.1 |
3D structure databases
SMRi | P54823 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199087, 44 interactors |
IntActi | P54823, 8 interactors |
MINTi | P54823 |
STRINGi | 10090.ENSMUSP00000128421 |
PTM databases
iPTMneti | P54823 |
PhosphoSitePlusi | P54823 |
SwissPalmi | P54823 |
Proteomic databases
EPDi | P54823 |
jPOSTi | P54823 |
MaxQBi | P54823 |
PaxDbi | P54823 |
PeptideAtlasi | P54823 |
PRIDEi | P54823 |
Protocols and materials databases
Antibodypediai | 3315, 415 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000170489; ENSMUSP00000128421; ENSMUSG00000032097 ENSMUST00000217034; ENSMUSP00000149620; ENSMUSG00000032097 |
GeneIDi | 13209 |
KEGGi | mmu:13209 |
UCSCi | uc009pdy.2, mouse |
Organism-specific databases
CTDi | 1656 |
MGIi | MGI:104976, Ddx6 |
Phylogenomic databases
eggNOGi | KOG0326, Eukaryota |
GeneTreei | ENSGT00900000141067 |
HOGENOMi | CLU_003041_30_0_1 |
InParanoidi | P54823 |
OMAi | DWNLMSS |
OrthoDBi | 583315at2759 |
PhylomeDBi | P54823 |
TreeFami | TF300440 |
Enzyme and pathway databases
Reactomei | R-MMU-430039, mRNA decay by 5' to 3' exoribonuclease |
Miscellaneous databases
BioGRID-ORCSi | 13209, 7 hits in 17 CRISPR screens |
ChiTaRSi | Ddx6, mouse |
PROi | PR:P54823 |
RNActi | P54823, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032097, Expressed in thymus and 305 other tissues |
Genevisiblei | P54823, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX6_MOUSE | |
Accessioni | P54823Primary (citable) accession number: P54823 Secondary accession number(s): O54979, Q3UFI3, Q8BW68 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families