UniProtKB - P54817 (SCAF_BHV1C)
Capsid scaffolding protein
UL26
Functioni
Acts as a scaffold protein by binding major capsid protein in the cytoplasm, inducing the nuclear localization of both proteins. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Autocatalytic cleavage releases the assembly protein, and subsequently abolishes interaction with major capsid protein. Cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationProtease that plays an essential role in virion assembly within the nucleus. Catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The cleavage sites seem to include -Ala-Ser-, -Ala-Ala-, as well as Ala-Thr bonds. Assemblin and cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationPlays a major role in capsid assembly. Acts as a scaffold protein by binding major capsid protein. Multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. Cleaved by assemblin after capsid completion. The cleavages products are evicted from the capsid before or during DNA packaging.
UniRule annotationCatalytic activityi
- Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.UniRule annotation EC:3.4.21.97
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 68 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 136 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 155 | Charge relay systemUniRule annotation | 1 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB-UniRule
- serine-type endopeptidase activity Source: UniProtKB-UniRule
GO - Biological processi
- viral process Source: AgBase
- viral release from host cell Source: UniProtKB-UniRule
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Viral capsid assembly, Viral release from host cell |
Protein family/group databases
MEROPSi | S21.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Capsid scaffolding proteinUniRule annotationAlternative name(s): Capsid protein P40 Protease precursorUniRule annotation Short name: pPRUniRule annotation Cleaved into the following 2 chains: Alternative name(s): ProteaseUniRule annotation Assembly proteinUniRule annotation Alternative name(s): Capsid assembly proteinUniRule annotation |
Gene namesi | Name:UL26 |
Organismi | Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine rhinotracheitis virus) |
Taxonomic identifieri | 10323 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Peploviricota › Herviviricetes › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › |
Virus hosti | Bos taurus (Bovine) [TaxID: 9913] |
Subcellular locationi
Other locations
- Host cytoplasm UniRule annotation
Other locations
- Host nucleus UniRule annotation
Other locations
- Host nucleus UniRule annotation
Other locations
- host cell cytoplasm Source: UniProtKB-SubCell
- host cell nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasm, Host nucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000027249 | 1 – 621 | Capsid scaffolding proteinAdd BLAST | 621 | |
ChainiPRO_0000027250 | 1 – 254 | AssemblinUniRule annotationAdd BLAST | 254 | |
ChainiPRO_0000027251 | 255 – 621 | Assembly proteinUniRule annotationAdd BLAST | 367 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 254 – 255 | Cleavage; by assemblin; Release siteUniRule annotation | 2 | |
Sitei | 596 – 597 | Cleavage; by assemblin; Tail siteUniRule annotation | 2 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Capsid scaffolding protein homomultimerizes and interacts with major capsid protein. Assemblin exists in a monomer-dimer equilibrium with the dimer being the active species. Assembly protein homomultimerizes and interacts with major capsid protein.
UniRule annotationGO - Molecular functioni
- identical protein binding Source: UniProtKB-UniRule
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 258 – 277 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 295 – 322 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 331 – 350 | Interaction with pAPUniRule annotationAdd BLAST | 20 | |
Regioni | 420 – 497 | DisorderedSequence analysisAdd BLAST | 78 | |
Regioni | 601 – 621 | Interaction with major capsid proteinUniRule annotationAdd BLAST | 21 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 439 – 442 | Nuclear localization signalBy similarity | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 299 – 315 | Pro residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 472 – 487 | Pro residuesSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.20.16.10, 1 hit |
HAMAPi | MF_04008, HSV_SCAF, 1 hit |
InterProi | View protein in InterPro IPR035443, Herpes_virus_sf IPR001847, Peptidase_S21 |
Pfami | View protein in Pfam PF00716, Peptidase_S21, 1 hit |
PRINTSi | PR00236, HSVCAPSIDP40 |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADAPDGGSA DARVDAEPSA LARASMPVYV GGYLALYGMG DEGELVLTRE
60 70 80 90 100
QVARALPPAA PLPINIDHAS ACEVGAVLAL ADDDAGLFFV GVINCPQLAD
110 120 130 140 150
TLAGVAHPAF FGADAPSLTP RERFLYLVSN YLPSVSLSSR RLAPDEEADG
160 170 180 190 200
TLFAHVALCV LGRRVGTIVT YDATPDACVA PFRRLSPRAR AALLANAEAA
210 220 230 240 250
RAALGDRAWP VPREALAQTL LSTAVNNMLV RDKWDTVSRR RREAGIAGHT
260 270 280 290 300
YLQASAVFPL PTGGEGPERT GGRERAQKSA VAGGVCIALP VAGGRARQPE
310 320 330 340 350
LPPAPPPPPP PPAMSAAHQA GAASAHPLPA GDYVYVPTAQ YNQLVVSQAR
360 370 380 390 400
GAAMTAAPPP APYFLPAAAA AAAAPPPMPG WYGAAGAAPW HPGYGFPPPG
410 420 430 440 450
LESQIMALAG AIADGRRVQA HGADGSGYDG PLDRRPLAKR RRYNWDHPRG
460 470 480 490 500
RSGGGDDDEA YYPGEGAPAE LPPHHHSPPP PHPPPSHALS KLASAVSSLQ
510 520 530 540 550
QEVSQLRAGY PYGPAFAAAQ HPPAAHLPCL PQQYTAPPRV GAGPAQVPTL
560 570 580 590 600
APAQAPAQAL SVPAVAAAPA TVAAAAAVGP PEEPGVAATV DASAMASLPP
610 620
AQPPQACDPA EIFVAQMMRQ R
The sequence of this isoform differs from the canonical sequence as follows:
1-313: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018864 | 1 – 313 | Missing in isoform pAP. CuratedAdd BLAST | 313 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31809 Genomic DNA Translation: AAA91999.1 U31809 Genomic DNA Translation: AAA92000.1 Z78205 Genomic DNA Translation: CAB01599.1 AJ004801 Genomic DNA Translation: CAA06107.1 |
RefSeqi | NP_045332.1, NC_001847.1 |
Genome annotation databases
GeneIDi | 4783434 |
KEGGi | vg:4783434 |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U31809 Genomic DNA Translation: AAA91999.1 U31809 Genomic DNA Translation: AAA92000.1 Z78205 Genomic DNA Translation: CAB01599.1 AJ004801 Genomic DNA Translation: CAA06107.1 |
RefSeqi | NP_045332.1, NC_001847.1 |
3D structure databases
SMRi | P54817 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | S21.001 |
Genome annotation databases
GeneIDi | 4783434 |
KEGGi | vg:4783434 |
Family and domain databases
Gene3Di | 3.20.16.10, 1 hit |
HAMAPi | MF_04008, HSV_SCAF, 1 hit |
InterProi | View protein in InterPro IPR035443, Herpes_virus_sf IPR001847, Peptidase_S21 |
Pfami | View protein in Pfam PF00716, Peptidase_S21, 1 hit |
PRINTSi | PR00236, HSVCAPSIDP40 |
MobiDBi | Search... |
Entry informationi
Entry namei | SCAF_BHV1C | |
Accessioni | P54817Primary (citable) accession number: P54817 Secondary accession number(s): Q89855 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | June 2, 2021 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |