Vacuolar protein sorting-associated protein 9

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• guanyl-nucleotide exchange factor activity Source: SGD <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport."
    Hama H., Tall G.G., Horazdovsky B.F.
    J. Biol. Chem. 274:15284-15291(1999) [PubMed] [Europe PMC] [Abstract]
  • "Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast."
    Paulsel A.L., Merz A.J., Nickerson D.P.
    J. Biol. Chem. 288:18162-18171(2013) [PubMed] [Europe PMC] [Abstract]
• ubiquitin binding Source: SGDInferred from direct assayⁱ
  • Ref.8

    "A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain."
    Shih S.C., Prag G., Francis S.A., Sutanto M.A., Hurley J.H., Hicke L.
    EMBO J. 22:1273-1281(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 398-449 IN COMPLEX WITH UBIQUITIN.

GO - Biological processⁱ

• Golgi to endosome transport Source: SGD <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3."
    Nickerson D.P., Russell M.R.G., Lo S.Y., Chapin H.C., Milnes J., Merz A.J.
    Traffic 13:1411-1428(2012) [PubMed] [Europe PMC] [Abstract]
  • "Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast."
    Paulsel A.L., Merz A.J., Nickerson D.P.
    J. Biol. Chem. 288:18162-18171(2013) [PubMed] [Europe PMC] [Abstract]
• late endosome to vacuole transport via multivesicular body sorting pathway Source: SGDInferred from mutant phenotypeⁱ
  • "Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast."
    Paulsel A.L., Merz A.J., Nickerson D.P.
    J. Biol. Chem. 288:18162-18171(2013) [PubMed] [Europe PMC] [Abstract]
• protein localization to endosome Source: SGD <p>Inferred from Genetic Interaction</p> <p>Used to describe “traditional” genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis."
    Cabrera M., Arlt H., Epp N., Lachmann J., Griffith J., Perz A., Reggiori F., Ungermann C.
    J. Biol. Chem. 288:5166-5175(2013) [PubMed] [Europe PMC] [Abstract]
• protein targeting to vacuole Source: SGDInferred from mutant phenotypeⁱ
  • Ref.1

    "A yeast protein related to a mammalian Ras-binding protein, Vps9p, is required for localization of vacuolar proteins."
    Burd C.G., Mustol P.A., Schu P.V., Emr S.D.
    Mol. Cell. Biol. 16:2369-2377(1996) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
• vacuole inheritance Source: SGDInferred from mutant phenotypeⁱ
  • "Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants."
    Raymond C.K., Howald-Stevenson I., Vater C.A., Stevens T.H.
    Mol. Biol. Cell 3:1389-1402(1992) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

GO - Molecular functionⁱ

• guanyl-nucleotide exchange factor activity Source: SGDInferred from direct assayⁱ
  • "Vps9p is a guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport."
    Hama H., Tall G.G., Horazdovsky B.F.
    J. Biol. Chem. 274:15284-15291(1999) [PubMed] [Europe PMC] [Abstract]
  • "Vps9 family protein Muk1 is the second Rab5 guanosine nucleotide exchange factor in budding yeast."
    Paulsel A.L., Merz A.J., Nickerson D.P.
    J. Biol. Chem. 288:18162-18171(2013) [PubMed] [Europe PMC] [Abstract]
• ubiquitin binding Source: SGDInferred from direct assayⁱ
  • Ref.8

    "A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain."
    Shih S.C., Prag G., Francis S.A., Sutanto M.A., Hurley J.H., Hicke L.
    EMBO J. 22:1273-1281(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 398-449 IN COMPLEX WITH UBIQUITIN.

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MTDDEKREIL KEFDPFSQLE QANGNPDKDV KFKKDDPNRA AAEETNRDIS 
        60         70         80         90        100
AQDKGDEEPF YDFQIFIKQL QTPGADPLVK YTKSFLRNFL AQRLLWTVSE 
       110        120        130        140        150
EIKLISDFKT FIYDKFTLYE PFRSLDNSKM RNAKEGMEKL IMGKLYSRCF 
       160        170        180        190        200
SPSLYEILQK PLDDEHMKDL TNDDTLLEKI RHYRFISPIM LDIPDTMPNA 
       210        220        230        240        250
RLNKFVHLAS KELGKINRFK SPRDKMVCVL NASKVIFGLL KHTKLEQNGA 
       260        270        280        290        300
DSFIPVLIYC ILKGQVRYLV SNVNYIERFR SPDFIRGEEE YYLSSLQAAL 
       310        320        330        340        350
NFIMNLTERS LTIEDHEDFE EAYQRNFKQL AEEKEEEEKK KQLEIPDELQ 
       360        370        380        390        400
PNGTLLKPLD EVTNIVISKF NELFSPIGEP TQEEALKSEQ SNKEEDVSSL 
       410        420        430        440        450
IKKIEENERK DTLNTLQNMF PDMDPSLIED VCIAKKSRIG PCVDALLSLS 

E                                                      

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Yeast
   Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
2. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
3. Yeast chromosome XIII
   Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names