The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P54787 (VPS9_YEAST)
Protein
Vacuolar protein sorting-associated protein 9
Gene
VPS9
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Required for vacuolar protein sorting; may be required for the consumption of transport vesicles containing vacuolar protein precursors. May bind a Rab GTPase such as VPS21.
Miscellaneous
Present with 4280 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: SGD
- small GTPase binding Source: GO_Central
- ubiquitin binding Source: SGD
GO - Biological processi
- Golgi to endosome transport Source: SGD
- late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
- protein localization to endosome Source: SGD
- protein targeting to vacuole Source: SGD
- vacuole inheritance Source: SGD
Keywordsi
Biological process | Ubl conjugation pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Vacuolar protein sorting-associated protein 9Alternative name(s): Vacuolar protein-targeting protein 9 |
Gene namesi | Name:VPS9 Synonyms:VPT9 Ordered Locus Names:YML097C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000004563, VPS9 |
VEuPathDBi | FungiDB:YML097C |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: SGD
Endosome
- early endosome Source: SGD
Other locations
- endocytic vesicle Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000191324 | 1 – 451 | Vacuolar protein sorting-associated protein 9Add BLAST | 451 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 375 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P54787 |
PaxDbi | P54787 |
PRIDEi | P54787 |
PTM databases
iPTMneti | P54787 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsGO - Molecular functioni
- small GTPase binding Source: GO_Central
- ubiquitin binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 35046, 583 interactors |
DIPi | DIP-4526N |
IntActi | P54787, 6 interactors |
MINTi | P54787 |
STRINGi | 4932.YML097C |
Miscellaneous databases
RNActi | P54787, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P54787 |
SMRi | P54787 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54787 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 170 – 312 | VPS9PROSITE-ProRule annotationAdd BLAST | 143 | |
Domaini | 408 – 451 | CUEPROSITE-ProRule annotationAdd BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 15 – 54 | DisorderedSequence analysisAdd BLAST | 40 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 229 – 237 | GBH motif I | 9 | |
Motifi | 249 – 257 | GBH motif II | 9 | |
Motifi | 287 – 295 | GBH motif III | 9 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 54 | Basic and acidic residuesSequence analysisAdd BLAST | 29 |
Domaini
The CUE domain (Coupling of ubiquitin conjugation to ER degradation) is monoubiquitin-binding and is required for intramolecular ubiquitination.
Phylogenomic databases
eggNOGi | KOG2319, Eukaryota |
GeneTreei | ENSGT00390000015057 |
HOGENOMi | CLU_007625_3_2_1 |
InParanoidi | P54787 |
OMAi | DVCIAKK |
Family and domain databases
CDDi | cd14369, CUE_VPS9_like, 1 hit |
Gene3Di | 1.20.1050.80, 1 hit |
InterProi | View protein in InterPro IPR003892, CUE IPR041545, DUF5601 IPR009060, UBA-like_sf IPR003123, VPS9 IPR045046, Vps9-like IPR041804, Vps9_CUE IPR037191, VPS9_dom_sf |
PANTHERi | PTHR23101, PTHR23101, 1 hit |
Pfami | View protein in Pfam PF02845, CUE, 1 hit PF18151, DUF5601, 1 hit PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00546, CUE, 1 hit SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51140, CUE, 1 hit PS51205, VPS9, 1 hit |
i Sequence
Sequence statusi: Complete.
P54787-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTDDEKREIL KEFDPFSQLE QANGNPDKDV KFKKDDPNRA AAEETNRDIS
60 70 80 90 100
AQDKGDEEPF YDFQIFIKQL QTPGADPLVK YTKSFLRNFL AQRLLWTVSE
110 120 130 140 150
EIKLISDFKT FIYDKFTLYE PFRSLDNSKM RNAKEGMEKL IMGKLYSRCF
160 170 180 190 200
SPSLYEILQK PLDDEHMKDL TNDDTLLEKI RHYRFISPIM LDIPDTMPNA
210 220 230 240 250
RLNKFVHLAS KELGKINRFK SPRDKMVCVL NASKVIFGLL KHTKLEQNGA
260 270 280 290 300
DSFIPVLIYC ILKGQVRYLV SNVNYIERFR SPDFIRGEEE YYLSSLQAAL
310 320 330 340 350
NFIMNLTERS LTIEDHEDFE EAYQRNFKQL AEEKEEEEKK KQLEIPDELQ
360 370 380 390 400
PNGTLLKPLD EVTNIVISKF NELFSPIGEP TQEEALKSEQ SNKEEDVSSL
410 420 430 440 450
IKKIEENERK DTLNTLQNMF PDMDPSLIED VCIAKKSRIG PCVDALLSLS
E
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 172 | N → T in AAA62233 (Ref. 2) Curated | 1 | |
Sequence conflicti | 305 | N → S in AAC49314 (PubMed:8628304).Curated | 1 | |
Sequence conflicti | 305 | N → S in AAA62233 (Ref. 2) Curated | 1 | |
Sequence conflicti | 314 | E → D in AAC49314 (PubMed:8628304).Curated | 1 | |
Sequence conflicti | 314 | E → D in AAA62233 (Ref. 2) Curated | 1 | |
Sequence conflicti | 327 | F → L in AAC49314 (PubMed:8628304).Curated | 1 | |
Sequence conflicti | 327 | F → L in AAA62233 (Ref. 2) Curated | 1 | |
Sequence conflicti | 345 | I → V in AAC49314 (PubMed:8628304).Curated | 1 | |
Sequence conflicti | 345 | I → V in AAA62233 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50142 Genomic DNA Translation: AAC49314.1 U20373 Genomic DNA Translation: AAA62233.1 Z46660 Genomic DNA Translation: CAA86640.1 BK006946 Genomic DNA Translation: DAA09802.1 |
PIRi | S49629 |
RefSeqi | NP_013612.1, NM_001182457.1 |
Genome annotation databases
EnsemblFungii | YML097C_mRNA; YML097C; YML097C |
GeneIDi | 854876 |
KEGGi | sce:YML097C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U50142 Genomic DNA Translation: AAC49314.1 U20373 Genomic DNA Translation: AAA62233.1 Z46660 Genomic DNA Translation: CAA86640.1 BK006946 Genomic DNA Translation: DAA09802.1 |
PIRi | S49629 |
RefSeqi | NP_013612.1, NM_001182457.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MN3 | X-ray | 2.30 | A | 398-451 | [»] | |
1P3Q | X-ray | 1.70 | Q/R | 398-451 | [»] | |
AlphaFoldDBi | P54787 | |||||
SMRi | P54787 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 35046, 583 interactors |
DIPi | DIP-4526N |
IntActi | P54787, 6 interactors |
MINTi | P54787 |
STRINGi | 4932.YML097C |
PTM databases
iPTMneti | P54787 |
Proteomic databases
MaxQBi | P54787 |
PaxDbi | P54787 |
PRIDEi | P54787 |
Genome annotation databases
EnsemblFungii | YML097C_mRNA; YML097C; YML097C |
GeneIDi | 854876 |
KEGGi | sce:YML097C |
Organism-specific databases
SGDi | S000004563, VPS9 |
VEuPathDBi | FungiDB:YML097C |
Phylogenomic databases
eggNOGi | KOG2319, Eukaryota |
GeneTreei | ENSGT00390000015057 |
HOGENOMi | CLU_007625_3_2_1 |
InParanoidi | P54787 |
OMAi | DVCIAKK |
Miscellaneous databases
EvolutionaryTracei | P54787 |
PROi | PR:P54787 |
RNActi | P54787, protein |
Family and domain databases
CDDi | cd14369, CUE_VPS9_like, 1 hit |
Gene3Di | 1.20.1050.80, 1 hit |
InterProi | View protein in InterPro IPR003892, CUE IPR041545, DUF5601 IPR009060, UBA-like_sf IPR003123, VPS9 IPR045046, Vps9-like IPR041804, Vps9_CUE IPR037191, VPS9_dom_sf |
PANTHERi | PTHR23101, PTHR23101, 1 hit |
Pfami | View protein in Pfam PF02845, CUE, 1 hit PF18151, DUF5601, 1 hit PF02204, VPS9, 1 hit |
SMARTi | View protein in SMART SM00546, CUE, 1 hit SM00167, VPS9, 1 hit |
SUPFAMi | SSF109993, SSF109993, 1 hit SSF46934, SSF46934, 1 hit |
PROSITEi | View protein in PROSITE PS51140, CUE, 1 hit PS51205, VPS9, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VPS9_YEAST | |
Accessioni | P54787Primary (citable) accession number: P54787 Secondary accession number(s): D6W0I8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 5, 2010 | |
Last modified: | May 25, 2022 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XIII
Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references