EPHA5

Functionⁱ

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).By similarity

Catalytic activityⁱ

ATP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
L-tyrosyl-[protein]
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
=
ADP
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
H⁺
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
+
O-phospho-L-tyrosyl-[protein]
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
PROSITE-ProRule annotation
Manual assertion according to rulesⁱ
- PROSITE-ProRule:PRU10028
EC:
2.7.10.1
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
PROSITE-ProRule annotation
Manual assertion according to rulesⁱ
- PROSITE-ProRule:PRU10028
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
ATP
+
L-tyrosyl-[protein]
L-tyrosine residue
zoom
=
ADP
+
H⁺
+
O-phospho-L-tyrosyl-[protein]
L-tyrosine-phosphate residue
zoom

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	707	ATPPROSITE-ProRule annotation		1
Active siteⁱ	800	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	681 – 689	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Receptor, Transferase, Tyrosine-protein kinase
Biological process	Neurogenesis
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.10.1 2681
Reactomeⁱ	R-HSA-2682334 EPH-Ephrin signaling R-HSA-3928663 EPHA-mediated growth cone collapse R-HSA-3928665 EPH-ephrin mediated repulsion of cells
SignaLinkⁱ	P54756
SIGNORⁱ	P54756

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ephrin type-A receptor 5 (EC:2.7.10.1) Alternative name(s): Brain-specific kinase EPH homology kinase 1 Short name: EHK-1 EPH-like kinase 7 Short name: EK7 Short name: hEK7
Gene namesⁱ	Name:EPHA5 Synonyms:BSK, EHK1, HEK7, TYRO4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000145242.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3389 EPHA5
MIMⁱ	600004 gene
neXtProtⁱ	NX_P54756

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	25 – 573	ExtracellularSequence analysisAdd BLAST	549
Transmembraneⁱ	574 – 594	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	595 – 1037	CytoplasmicSequence analysisAdd BLAST	443

Keywords - Cellular componentⁱ

Cell membrane, Cell projection, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	2044
Open Targets More...OpenTargetsⁱ	ENSG00000145242
PharmGKBⁱ	PA27821

Miscellaneous databases

Pharosⁱ	P54756 Tchem

Pharosⁱ

P54756 Tchem

Chemistry databases

ChEMBLⁱ	CHEMBL3987
Drug and drug target database More...DrugBankⁱ	DB01254 Dasatinib DB12010 Fostamatinib
DrugCentral More...DrugCentralⁱ	P54756
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	1825

Polymorphism and mutation databases

BioMutaⁱ	EPHA5
DMDMⁱ	259016353

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 24	Sequence analysisAdd BLAST		24
ChainⁱPRO_0000016812	25 – 1037	Ephrin type-A receptor 5Add BLAST		1013

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	264	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	299	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	369	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	423	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	436	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	461	N-linked (GlcNAc...) asparagineSequence analysis	1
Modified residueⁱ	650	Phosphotyrosine; by autocatalysisBy similarity	1
Modified residueⁱ	656	Phosphotyrosine; by autocatalysisBy similarity	1
Modified residueⁱ	833	Phosphotyrosine; by autocatalysisSequence analysis	1
Modified residueⁱ	982	Phosphotyrosine; by autocatalysisBy similarity	1

Post-translational modificationⁱ

Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion (By similarity).By similarity

Keywords - PTMⁱ

Glycoprotein, Phosphoprotein

Proteomic databases

EPDⁱ	P54756
jPOSTⁱ	P54756
MassIVEⁱ	P54756
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P54756
PaxDbⁱ	P54756
PeptideAtlas More...PeptideAtlasⁱ	P54756
PRIDEⁱ	P54756
ProteomicsDBⁱ	56712 [P54756-1] 56713 [P54756-2] 56714 [P54756-3]
TopDownProteomicsⁱ	P54756-2 [P54756-2]

PTM databases

iPTMnetⁱ	P54756
PhosphoSitePlusⁱ	P54756

Expressionⁱ

Tissue specificityⁱ

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000145242 Expressed in 96 organ(s), highest expression level in frontal cortex
ExpressionAtlasⁱ	P54756 baseline and differential
Genevisibleⁱ	P54756 HS

Interactionⁱ

Subunit structureⁱ

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Interacts (via SAM domain) with SAMD5 (via SAM domain) (By similarity).

By similarity

Protein-protein interaction databases

BioGridⁱ	108358, 9 interactors
IntActⁱ	P54756, 6 interactors
Molecular INTeraction database More...MINTⁱ	P54756
STRINGⁱ	9606.ENSP00000480763

Chemistry databases

BindingDBⁱ

P54756

Miscellaneous databases

RNActⁱ	P54756 protein

RNActⁱ

P54756 protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	61 – 65	Combined sources	5
Turnⁱ	66 – 68	Combined sources	3
Beta strandⁱ	76 – 89	Combined sources	14
Beta strandⁱ	95 – 101	Combined sources	7
Beta strandⁱ	105 – 107	Combined sources	3
Beta strandⁱ	111 – 114	Combined sources	4
Beta strandⁱ	126 – 135	Combined sources	10
Helixⁱ	137 – 139	Combined sources	3
Beta strandⁱ	148 – 157	Combined sources	10
Helixⁱ	166 – 168	Combined sources	3
Beta strandⁱ	172 – 178	Combined sources	7
Beta strandⁱ	188 – 192	Combined sources	5
Beta strandⁱ	196 – 202	Combined sources	7
Beta strandⁱ	207 – 219	Combined sources	13
Beta strandⁱ	221 – 230	Combined sources	10
Helixⁱ	663 – 665	Combined sources	3
Helixⁱ	672 – 674	Combined sources	3
Beta strandⁱ	675 – 683	Combined sources	9
Beta strandⁱ	685 – 694	Combined sources	10
Beta strandⁱ	702 – 708	Combined sources	7
Helixⁱ	715 – 728	Combined sources	14
Beta strandⁱ	739 – 743	Combined sources	5
Beta strandⁱ	745 – 748	Combined sources	4
Beta strandⁱ	750 – 754	Combined sources	5
Helixⁱ	761 – 766	Combined sources	6
Turnⁱ	767 – 770	Combined sources	4
Helixⁱ	774 – 793	Combined sources	20
Helixⁱ	803 – 805	Combined sources	3
Beta strandⁱ	806 – 808	Combined sources	3
Beta strandⁱ	814 – 816	Combined sources	3
Helixⁱ	842 – 844	Combined sources	3
Helixⁱ	847 – 852	Combined sources	6
Helixⁱ	857 – 873	Combined sources	17
Turnⁱ	878 – 881	Combined sources	4
Helixⁱ	884 – 893	Combined sources	10
Helixⁱ	905 – 914	Combined sources	10
Helixⁱ	919 – 921	Combined sources	3
Helixⁱ	925 – 936	Combined sources	12

Show more details

3D structure databases

SMRⁱ	P54756
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	P54756

EvolutionaryTraceⁱ

P54756

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	60 – 238	Eph LBDPROSITE-ProRule annotationAdd BLAST	179
Domainⁱ	357 – 467	Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST	111
Domainⁱ	468 – 562	Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST	95
Domainⁱ	675 – 936	Protein kinasePROSITE-ProRule annotationAdd BLAST	262
Domainⁱ	965 – 1029	SAMPROSITE-ProRule annotationAdd BLAST	65

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	1035 – 1037	PDZ-bindingSequence analysis		3

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	220 – 354	Cys-richAdd BLAST		135

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation

Keywords - Domainⁱ

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0196 Eukaryota COG0515 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000156266
InParanoidⁱ	P54756
KEGG Orthology (KO) More...KOⁱ	K05106
Database of Orthologous Groups More...OrthoDBⁱ	933071at2759
PhylomeDBⁱ	P54756
TreeFamⁱ	TF315608

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd10483 EphR_LBD_A5, 1 hit cd00063 FN3, 2 hits
Gene3Dⁱ	1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR027936 Eph_TM IPR034277 EphA5_rcpt_lig-bd IPR001090 Ephrin_rcpt_lig-bd_dom IPR003961 FN3_dom IPR036116 FN3_sf IPR008979 Galactose-bd-like_sf IPR013783 Ig-like_fold IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001660 SAM IPR013761 SAM/pointed_sf IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR011641 Tyr-kin_ephrin_A/B_rcpt-like IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom IPR016257 Tyr_kinase_ephrin_rcpt IPR001426 Tyr_kinase_rcpt_V_CS
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF14575 EphA2_TM, 1 hit PF01404 Ephrin_lbd, 1 hit PF00041 fn3, 2 hits PF07714 Pkinase_Tyr, 1 hit PF00536 SAM_1, 1 hit
PIRSFⁱ	PIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00615 EPH_lbd, 1 hit SM01411 Ephrin_rec_like, 1 hit SM00060 FN3, 2 hits SM00454 SAM, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF49265 SSF49265, 1 hit SSF49785 SSF49785, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS01186 EGF_2, 1 hit PS51550 EPH_LBD, 1 hit PS50853 FN3, 2 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit PS50105 SAM_DOMAIN, 1 hit

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Additional isoforms seem to exist.

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: P54756-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MRGSGPRGAG RRRPPSGGGD TPITPASLAG CYSAPRRAPL WTCLLLCAAL 
        60         70         80         90        100
RTLLASPSNE VNLLDSRTVM GDLGWIAFPK NGWEEIGEVD ENYAPIHTYQ 
       110        120        130        140        150
VCKVMEQNQN NWLLTSWISN EGASRIFIEL KFTLRDCNSL PGGLGTCKET 
       160        170        180        190        200
FNMYYFESDD QNGRNIKENQ YIKIDTIAAD ESFTELDLGD RVMKLNTEVR 
       210        220        230        240        250
DVGPLSKKGF YLAFQDVGAC IALVSVRVYY KKCPSVVRHL AVFPDTITGA 
       260        270        280        290        300
DSSQLLEVSG SCVNHSVTDE PPKMHCSAEG EWLVPIGKCM CKAGYEEKNG 
       310        320        330        340        350
TCQVCRPGFF KASPHIQSCG KCPPHSYTHE EASTSCVCEK DYFRRESDPP 
       360        370        380        390        400
TMACTRPPSA PRNAISNVNE TSVFLEWIPP ADTGGRKDVS YYIACKKCNS 
       410        420        430        440        450
HAGVCEECGG HVRYLPRQSG LKNTSVMMVD LLAHTNYTFE IEAVNGVSDL 
       460        470        480        490        500
SPGARQYVSV NVTTNQAAPS PVTNVKKGKI AKNSISLSWQ EPDRPNGIIL 
       510        520        530        540        550
EYEIKYFEKD QETSYTIIKS KETTITAEGL KPASVYVFQI RARTAAGYGV 
       560        570        580        590        600
FSRRFEFETT PVFAASSDQS QIPVIAVSVT VGVILLAVVI GVLLSGSCCE 
       610        620        630        640        650
CGCGRASSLC AVAHPSLIWR CGYSKAKQDP EEEKMHFHNG HIKLPGVRTY 
       660        670        680        690        700
IDPHTYEDPN QAVHEFAKEI EASCITIERV IGAGEFGEVC SGRLKLPGKR 
       710        720        730        740        750
ELPVAIKTLK VGYTEKQRRD FLGEASIMGQ FDHPNIIHLE GVVTKSKPVM 
       760        770        780        790        800
IVTEYMENGS LDTFLKKNDG QFTVIQLVGM LRGISAGMKY LSDMGYVHRD 
       810        820        830        840        850
LAARNILINS NLVCKVSDFG LSRVLEDDPE AAYTTRGGKI PIRWTAPEAI 
       860        870        880        890        900
AFRKFTSASD VWSYGIVMWE VVSYGERPYW EMTNQDVIKA VEEGYRLPSP 
       910        920        930        940        950
MDCPAALYQL MLDCWQKERN SRPKFDEIVN MLDKLIRNPS SLKTLVNASC 
       960        970        980        990       1000
RVSNLLAEHS PLGSGAYRSV GEWLEAIKMG RYTEIFMENG YSSMDAVAQV 
      1010       1020       1030 
TLEDLRRLGV TLVGHQKKIM NSLQEMKVQL VNGMVPL

Length:1,037

Mass (Da):114,803

Last modified:September 22, 2009 - v3

Checksum:ⁱ578C2F4D950DE419

GO

Isoform 2 (identifier: P54756-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
597-619: SCCECGCGRASSLCAVAHPSLIW → R

« Hide

        10         20         30         40         50
MRGSGPRGAG RRRPPSGGGD TPITPASLAG CYSAPRRAPL WTCLLLCAAL 
        60         70         80         90        100
RTLLASPSNE VNLLDSRTVM GDLGWIAFPK NGWEEIGEVD ENYAPIHTYQ 
       110        120        130        140        150
VCKVMEQNQN NWLLTSWISN EGASRIFIEL KFTLRDCNSL PGGLGTCKET 
       160        170        180        190        200
FNMYYFESDD QNGRNIKENQ YIKIDTIAAD ESFTELDLGD RVMKLNTEVR 
       210        220        230        240        250
DVGPLSKKGF YLAFQDVGAC IALVSVRVYY KKCPSVVRHL AVFPDTITGA 
       260        270        280        290        300
DSSQLLEVSG SCVNHSVTDE PPKMHCSAEG EWLVPIGKCM CKAGYEEKNG 
       310        320        330        340        350
TCQVCRPGFF KASPHIQSCG KCPPHSYTHE EASTSCVCEK DYFRRESDPP 
       360        370        380        390        400
TMACTRPPSA PRNAISNVNE TSVFLEWIPP ADTGGRKDVS YYIACKKCNS 
       410        420        430        440        450
HAGVCEECGG HVRYLPRQSG LKNTSVMMVD LLAHTNYTFE IEAVNGVSDL 
       460        470        480        490        500
SPGARQYVSV NVTTNQAAPS PVTNVKKGKI AKNSISLSWQ EPDRPNGIIL 
       510        520        530        540        550
EYEIKYFEKD QETSYTIIKS KETTITAEGL KPASVYVFQI RARTAAGYGV 
       560        570        580        590        600
FSRRFEFETT PVFAASSDQS QIPVIAVSVT VGVILLAVVI GVLLSGRRCG 
       610        620        630        640        650
YSKAKQDPEE EKMHFHNGHI KLPGVRTYID PHTYEDPNQA VHEFAKEIEA 
       660        670        680        690        700
SCITIERVIG AGEFGEVCSG RLKLPGKREL PVAIKTLKVG YTEKQRRDFL 
       710        720        730        740        750
GEASIMGQFD HPNIIHLEGV VTKSKPVMIV TEYMENGSLD TFLKKNDGQF 
       760        770        780        790        800
TVIQLVGMLR GISAGMKYLS DMGYVHRDLA ARNILINSNL VCKVSDFGLS 
       810        820        830        840        850
RVLEDDPEAA YTTRGGKIPI RWTAPEAIAF RKFTSASDVW SYGIVMWEVV 
       860        870        880        890        900
SYGERPYWEM TNQDVIKAVE EGYRLPSPMD CPAALYQLML DCWQKERNSR 
       910        920        930        940        950
PKFDEIVNML DKLIRNPSSL KTLVNASCRV SNLLAEHSPL GSGAYRSVGE 
       960        970        980        990       1000
WLEAIKMGRY TEIFMENGYS SMDAVAQVTL EDLRRLGVTL VGHQKKIMNS 
      1010 
LQEMKVQLVN GMVPL

Show »

Length:1,015

Mass (Da):112,624

Checksum:ⁱ0C0121184BCE631E

GO

Isoform 3 (identifier: P54756-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-69: Missing.
563-563: F → SV

« Hide

        10         20         30         40         50
MGDLGWIAFP KNGWEEIGEV DENYAPIHTY QVCKVMEQNQ NNWLLTSWIS 
        60         70         80         90        100
NEGASRIFIE LKFTLRDCNS LPGGLGTCKE TFNMYYFESD DQNGRNIKEN 
       110        120        130        140        150
QYIKIDTIAA DESFTELDLG DRVMKLNTEV RDVGPLSKKG FYLAFQDVGA 
       160        170        180        190        200
CIALVSVRVY YKKCPSVVRH LAVFPDTITG ADSSQLLEVS GSCVNHSVTD 
       210        220        230        240        250
EPPKMHCSAE GEWLVPIGKC MCKAGYEEKN GTCQVCRPGF FKASPHIQSC 
       260        270        280        290        300
GKCPPHSYTH EEASTSCVCE KDYFRRESDP PTMACTRPPS APRNAISNVN 
       310        320        330        340        350
ETSVFLEWIP PADTGGRKDV SYYIACKKCN SHAGVCEECG GHVRYLPRQS 
       360        370        380        390        400
GLKNTSVMMV DLLAHTNYTF EIEAVNGVSD LSPGARQYVS VNVTTNQAAP 
       410        420        430        440        450
SPVTNVKKGK IAKNSISLSW QEPDRPNGII LEYEIKYFEK DQETSYTIIK 
       460        470        480        490        500
SKETTITAEG LKPASVYVFQ IRARTAAGYG VFSRRFEFET TPVSVAASSD 
       510        520        530        540        550
QSQIPVIAVS VTVGVILLAV VIGVLLSGSC CECGCGRASS LCAVAHPSLI 
       560        570        580        590        600
WRCGYSKAKQ DPEEEKMHFH NGHIKLPGVR TYIDPHTYED PNQAVHEFAK 
       610        620        630        640        650
EIEASCITIE RVIGAGEFGE VCSGRLKLPG KRELPVAIKT LKVGYTEKQR 
       660        670        680        690        700
RDFLGEASIM GQFDHPNIIH LEGVVTKSKP VMIVTEYMEN GSLDTFLKKN 
       710        720        730        740        750
DGQFTVIQLV GMLRGISAGM KYLSDMGYVH RDLAARNILI NSNLVCKVSD 
       760        770        780        790        800
FGLSRVLEDD PEAAYTTRGG KIPIRWTAPE AIAFRKFTSA SDVWSYGIVM 
       810        820        830        840        850
WEVVSYGERP YWEMTNQDVI KAVEEGYRLP SPMDCPAALY QLMLDCWQKE 
       860        870        880        890        900
RNSRPKFDEI VNMLDKLIRN PSSLKTLVNA SCRVSNLLAE HSPLGSGAYR 
       910        920        930        940        950
SVGEWLEAIK MGRYTEIFME NGYSSMDAVA QVTLEDLRRL GVTLVGHQKK 
       960 
IMNSLQEMKV QLVNGMVPL

Show »

Length:969

Mass (Da):107,699

Checksum:ⁱ3038A240682F6E84

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

B7ZKJ3	B7ZKJ3_HUMAN	EPH receptor A5 EPH receptor A5 (EPH receptor A5, isoform CRA_b) (EPHA5 protein) (Ephrin type-A receptor 5)	EPHA5 hCG_1810817	1,038	Annotation score:
B7ZKW7	B7ZKW7_HUMAN	EPHA5 protein EPHA5 protein (Ephrin type-A receptor 5)	EPHA5	1,016	Annotation score:
F8VP57	F8VP57_HUMAN	Ephrin type-A receptor 5 Ephrin type-A receptor 5	EPHA5	1,004	Annotation score:
F8W9W0	F8W9W0_HUMAN	Ephrin type-A receptor 5 Ephrin type-A receptor 5	EPHA5	874	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	11	R → H in CAA64700 (PubMed:9191074).Curated	1
Sequence conflictⁱ	87	G → D in CAD97914 (PubMed:17974005).Curated	1
Sequence conflictⁱ	506	Y → H in CAA64700 (PubMed:9191074).Curated	1
Sequence conflictⁱ	611	A → V in CAD97914 (PubMed:17974005).Curated	1
Sequence conflictⁱ	616	S → I in CAA64700 (PubMed:9191074).Curated	1
Sequence conflictⁱ	820	G → E in CAD97914 (PubMed:17974005).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_042138	81	N → T1 PublicationCorresponds to variant dbSNP:rs33932471Ensembl.	1
Natural variantⁱVAR_042139	235	S → A1 PublicationCorresponds to variant dbSNP:rs55710198Ensembl.	1
Natural variantⁱVAR_042140	330	E → Q1 PublicationCorresponds to variant dbSNP:rs56205382Ensembl.	1
Natural variantⁱVAR_042141	417	R → Q in a lung adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs199614818Ensembl.	1
Natural variantⁱVAR_042142	503	E → K in a lung large cell carcinoma sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_045912	506	Y → C1 PublicationCorresponds to variant dbSNP:rs56074660Ensembl.	1
Natural variantⁱVAR_042143	582	G → E in a lung adenocarcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1057520012EnsemblClinVar.	1
Natural variantⁱVAR_042144	672	A → T1 PublicationCorresponds to variant dbSNP:rs36050417Ensembl.	1
Natural variantⁱVAR_042145	673	S → T1 PublicationCorresponds to variant dbSNP:rs56359290Ensembl.	1
Natural variantⁱVAR_042146	856	T → I in a lung squamous cell carcinoma sample; somatic mutation. 1 Publication	1
Natural variantⁱVAR_042147	959	H → R1 PublicationCorresponds to variant dbSNP:rs56312931Ensembl.	1
Natural variantⁱVAR_042148	1032	N → S in a lung large cell carcinoma sample; somatic mutation. 1 Publication	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_039118	1 – 69	Missing in isoform 3. 1 PublicationAdd BLAST	69
Alternative sequenceⁱVSP_039119	563	F → SV in isoform 3. 1 Publication	1
Alternative sequenceⁱVSP_002999	597 – 619	SCCEC…PSLIW → R in isoform 2. CuratedAdd BLAST	23

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X95425 mRNA Translation: CAA64700.1 AC018683 Genomic DNA No translation available. AC104137 Genomic DNA No translation available. AC105923 Genomic DNA No translation available. AC115223 Genomic DNA No translation available. BX537946 mRNA Translation: CAD97914.1 L36644 mRNA Translation: AAA74245.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS3513.1 [P54756-1] CCDS3514.1 [P54756-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001268694.1, NM_001281765.2 NP_001268695.1, NM_001281766.2 NP_001305690.1, NM_001318761.1 [P54756-3] NP_004430.4, NM_004439.7 [P54756-1] NP_872272.2, NM_182472.4 [P54756-2]

Genome annotation databases

Ensemblⁱ	ENST00000273854; ENSP00000273854; ENSG00000145242 [P54756-1] ENST00000354839; ENSP00000346899; ENSG00000145242 [P54756-2]
GeneIDⁱ	2044
KEGGⁱ	hsa:2044
UCSC genome browser More...UCSCⁱ	uc003hcy.5 human [P54756-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P54756	EPH receptor A5, isoform CRA_a		HUMAN		1037	UniRef100_P54756
P54756-3	EPH receptor A5		HUMAN		1038	UniRef100_B7ZKJ3
Ephrin type-A receptor 5		HUMAN		1004

Protein	Similar proteins		Species	Score	Length	Source
P54756	EPH receptor A5, isoform CRA_a		HUMAN		1037	UniRef90_P54756
Uncharacterized protein		wild yak		1228
Protein kinase domain-containing protein (Fragment)		NEOLE		90
Tyrosine kinase receptor (Fragment)		MOUSE		57
EPH receptor A5		CHLSB		1042
+286
P54756-3	EPH receptor A5		GORGO		1038	UniRef90_G3RK16
EPH receptor A5		HUMAN		1038
EPH receptor A5		PANTR		874
Ephrin type-A receptor 5		HUMAN		1004
Ephrin receptor EphA5 isoform a variant (Fragment)		HUMAN		1017
+4

Protein	Similar proteins		Species	Score	Length	Source
P54756	Ephrin type-A receptor 5	)	MOUSE		876	UniRef50_Q60629
Ephrin type-A receptor 5		CHICK		1013
Ephrin type-A receptor 5		RAT		1005
Ephrin type-A receptor 2 (Fragment)		CALAN		328
Uncharacterized protein		ESOLU		1001
+999

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X95425 mRNA Translation: CAA64700.1 AC018683 Genomic DNA No translation available. AC104137 Genomic DNA No translation available. AC105923 Genomic DNA No translation available. AC115223 Genomic DNA No translation available. BX537946 mRNA Translation: CAD97914.1 L36644 mRNA Translation: AAA74245.1
CCDSⁱ	CCDS3513.1 [P54756-1] CCDS3514.1 [P54756-2]
RefSeqⁱ	NP_001268694.1, NM_001281765.2 NP_001268695.1, NM_001281766.2 NP_001305690.1, NM_001318761.1 [P54756-3] NP_004430.4, NM_004439.7 [P54756-1] NP_872272.2, NM_182472.4 [P54756-2]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2R2P	X-ray	2.40	A	653-939	[»]
	4ET7	X-ray	2.60	A	59-235	[»]
SMRⁱ	P54756
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	108358, 9 interactors
IntActⁱ	P54756, 6 interactors
MINTⁱ	P54756
STRINGⁱ	9606.ENSP00000480763

Chemistry databases

BindingDBⁱ	P54756
ChEMBLⁱ	CHEMBL3987
DrugBankⁱ	DB01254 Dasatinib DB12010 Fostamatinib
DrugCentralⁱ	P54756
GuidetoPHARMACOLOGYⁱ	1825

PTM databases

iPTMnetⁱ	P54756
PhosphoSitePlusⁱ	P54756

Polymorphism and mutation databases

BioMutaⁱ	EPHA5
DMDMⁱ	259016353

Proteomic databases

EPDⁱ	P54756
jPOSTⁱ	P54756
MassIVEⁱ	P54756
MaxQBⁱ	P54756
PaxDbⁱ	P54756
PeptideAtlasⁱ	P54756
PRIDEⁱ	P54756
ProteomicsDBⁱ	56712 [P54756-1] 56713 [P54756-2] 56714 [P54756-3]
TopDownProteomicsⁱ	P54756-2 [P54756-2]

Protocols and materials databases

ABCDⁱ	P54756

ABCDⁱ

P54756

Genome annotation databases

Ensemblⁱ	ENST00000273854; ENSP00000273854; ENSG00000145242 [P54756-1] ENST00000354839; ENSP00000346899; ENSG00000145242 [P54756-2]
GeneIDⁱ	2044
KEGGⁱ	hsa:2044
UCSCⁱ	uc003hcy.5 human [P54756-1]

Organism-specific databases

CTDⁱ	2044
DisGeNETⁱ	2044
EuPathDBⁱ	HostDB:ENSG00000145242.13
GeneCardsⁱ	EPHA5
HGNCⁱ	HGNC:3389 EPHA5
MIMⁱ	600004 gene
neXtProtⁱ	NX_P54756
OpenTargetsⁱ	ENSG00000145242
PharmGKBⁱ	PA27821
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0196 Eukaryota COG0515 LUCA
GeneTreeⁱ	ENSGT00940000156266
InParanoidⁱ	P54756
KOⁱ	K05106
OrthoDBⁱ	933071at2759
PhylomeDBⁱ	P54756
TreeFamⁱ	TF315608

Enzyme and pathway databases

BRENDAⁱ	2.7.10.1 2681
Reactomeⁱ	R-HSA-2682334 EPH-Ephrin signaling R-HSA-3928663 EPHA-mediated growth cone collapse R-HSA-3928665 EPH-ephrin mediated repulsion of cells
SignaLinkⁱ	P54756
SIGNORⁱ	P54756

Miscellaneous databases

ChiTaRSⁱ	EPHA5 human
EvolutionaryTraceⁱ	P54756
GeneWikiⁱ	EPH_receptor_A5
GenomeRNAiⁱ	2044
Pharosⁱ	P54756 Tchem
Protein Ontology More...PROⁱ	PR:P54756
RNActⁱ	P54756 protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000145242 Expressed in 96 organ(s), highest expression level in frontal cortex
ExpressionAtlasⁱ	P54756 baseline and differential
Genevisibleⁱ	P54756 HS

Family and domain databases

CDDⁱ	cd10483 EphR_LBD_A5, 1 hit cd00063 FN3, 2 hits
Gene3Dⁱ	1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR027936 Eph_TM IPR034277 EphA5_rcpt_lig-bd IPR001090 Ephrin_rcpt_lig-bd_dom IPR003961 FN3_dom IPR036116 FN3_sf IPR008979 Galactose-bd-like_sf IPR013783 Ig-like_fold IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001660 SAM IPR013761 SAM/pointed_sf IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR011641 Tyr-kin_ephrin_A/B_rcpt-like IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom IPR016257 Tyr_kinase_ephrin_rcpt IPR001426 Tyr_kinase_rcpt_V_CS
Pfamⁱ	View protein in Pfam PF14575 EphA2_TM, 1 hit PF01404 Ephrin_lbd, 1 hit PF00041 fn3, 2 hits PF07714 Pkinase_Tyr, 1 hit PF00536 SAM_1, 1 hit
PIRSFⁱ	PIRSF000666 TyrPK_ephrin_receptor, 1 hit
PRINTSⁱ	PR00109 TYRKINASE
SMARTⁱ	View protein in SMART SM00615 EPH_lbd, 1 hit SM01411 Ephrin_rec_like, 1 hit SM00060 FN3, 2 hits SM00454 SAM, 1 hit SM00219 TyrKc, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF49265 SSF49265, 1 hit SSF49785 SSF49785, 1 hit SSF56112 SSF56112, 1 hit
PROSITEⁱ	View protein in PROSITE PS01186 EGF_2, 1 hit PS51550 EPH_LBD, 1 hit PS50853 FN3, 2 hits PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS00790 RECEPTOR_TYR_KIN_V_1, 1 hit PS00791 RECEPTOR_TYR_KIN_V_2, 1 hit PS50105 SAM_DOMAIN, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EPHA5_HUMAN
Accessionⁱ	P54756Primary (citable) accession number: P54756 Secondary accession number(s): Q7Z3F2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	September 22, 2009
	Last modified:	December 11, 2019
	This is version 199 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human and mouse protein kinases
Human and mouse protein kinases: classification and index

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UniProtKB - P54756 (EPHA5_HUMAN)

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Ephrin type-A receptor 5

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

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Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

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3D structure databases

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<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

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Experimental Info

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Sequence databases

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Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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Protein-protein interaction databases

Chemistry databases

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ