UniProtKB - P54755 (EPHA5_CHICK)
Protein
Ephrin type-A receptor 5
Gene
EPHA5
Organism
Gallus gallus (Chicken)
Status
Functioni
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis (By similarity).By similarity
Catalytic activityi
- EC:2.7.10.1PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 683 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 776 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 657 – 665 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- GPI-linked ephrin receptor activity Source: UniProtKB
- transmembrane-ephrin receptor activity Source: GO_Central
- transmembrane receptor protein tyrosine kinase activity Source: GO_Central
GO - Biological processi
- axon guidance Source: UniProtKB
- ephrin receptor signaling pathway Source: UniProtKB
- hippocampus development Source: UniProtKB
- multicellular organism development Source: GO_Central
- positive regulation of kinase activity Source: GO_Central
- regulation of actin cytoskeleton organization Source: UniProtKB
- regulation of GTPase activity Source: UniProtKB
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Kinase, Receptor, Transferase, Tyrosine-protein kinase |
Biological process | Neurogenesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 1306 |
Names & Taxonomyi
Protein namesi | Recommended name: Ephrin type-A receptor 5 (EC:2.7.10.1)Alternative name(s): EPH-like kinase 7 Short name: EK7 Short name: cEK7 |
Gene namesi | Name:EPHA5 Synonyms:CEK7 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Other locations
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- axon Source: UniProtKB
- dendrite Source: UniProtKB
- neuron projection Source: GO_Central
- receptor complex Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 32 – 549 | ExtracellularSequence analysisAdd BLAST | 518 | |
Transmembranei | 550 – 570 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 571 – 1013 | CytoplasmicSequence analysisAdd BLAST | 443 |
Keywords - Cellular componenti
Cell membrane, Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | Sequence analysisAdd BLAST | 31 | |
ChainiPRO_0000016815 | 32 – 1013 | Ephrin type-A receptor 5Add BLAST | 982 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 240 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 275 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 345 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 399 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 412 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 437 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 626 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 632 | Phosphotyrosine; by autocatalysisBy similarity | 1 | |
Modified residuei | 809 | Phosphotyrosine; by autocatalysisSequence analysis | 1 | |
Modified residuei | 958 | Phosphotyrosine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5 (By similarity).By similarity
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P54755 |
Expressioni
Tissue specificityi
Detected in the 10-day embryonic brain, weaker expression in the rest of the 10-day embryo. Undetected in adult tissues.
Interactioni
Subunit structurei
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9031.ENSGALP00000037020 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 214 | Eph LBDPROSITE-ProRule annotationAdd BLAST | 179 | |
Domaini | 333 – 443 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 111 | |
Domaini | 444 – 538 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 651 – 912 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 262 | |
Domaini | 941 – 1013 | SAMPROSITE-ProRule annotationAdd BLAST | 73 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1011 – 1013 | PDZ-bindingSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 196 – 330 | Cys-richAdd BLAST | 135 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P54755 |
OMAi | ECGPNVR |
OrthoDBi | 933071at2759 |
PhylomeDBi | P54755 |
Family and domain databases
CDDi | cd00063, FN3, 2 hits |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
Isoform 3 (identifier: P54755-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGLRGGGGRA GGPAPGWTCL LLCAALRSLL ASPGSEVNLL DSRTVMGDLG
60 70 80 90 100
WIAYPKNGWE EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGRP
110 120 130 140 150
ASSFELKFTL RDCNSLPGGL GTCKETFNMY YFESDDEDGR NIRENQYIKI
160 170 180 190 200
DTIAADESFT ELDLGDRVMK LNTEVRDVGP LTKKGFYLAF QDVGACIALV
210 220 230 240 250
SVRVYYKKCP SVIRNLARFP DTITGADSSQ LLEVSGVCVN HSVTDEAPKM
260 270 280 290 300
HCSAEGEWLV PIGKCLCKAG YEEKNNTCQV CRPGFFKASP HSPSCSKCPP
310 320 330 340 350
HSYTLDEAST SCLCEEHYFR RESDPPTMAC TRPPSAPRSA ISNVNETSVF
360 370 380 390 400
LEWIPPADTG GRKDVSYYIA CKKCNSHSGL CEACGSHVRY LPQQTGLKNT
410 420 430 440 450
SVMMVDLLAH TNYTFEIEAV NGVSDQNPGA RQFVSVNVTT NQAAPSPVSS
460 470 480 490 500
VKKGKITKNS ISLSWQEPDR PNGIILEYEI KYFEKDQETS YTIIKSKETA
510 520 530 540 550
ITADGLKPGS AYVFQIRART AAGYGGFSRR FEFETSPVLA ASSDQSQIPI
560 570 580 590 600
IVVSVTVGVI LLAVVIGFLL SGSCCDHGCG WASSLRAVAY PSLIWRCGYS
610 620 630 640 650
KAKQDPEEEK MHFHNGHIKL PGVRTYIDPH TYEDPNQAVH EFAKEIEASC
660 670 680 690 700
ITIERVIGAG EFGEVCSGRL KLQGKREFPV AIKTLKVGYT EKQRRDFLGE
710 720 730 740 750
ASIMGQFDHP NIIHLEGVVT KSKPVMIVTE YMENGSLDTF LKKNDGQFTV
760 770 780 790 800
IQLVGMLRGI ASGMKYLSDM GYVHRDLAAR NILINSNLVC KVSDFGLSRV
810 820 830 840 850
LEDDPEAAYT TRGGKIPIRW TAPEAIAFRK FTSASDVWSY GIVMWEVMSY
860 870 880 890 900
GERPYWEMTN QDVIKAVEEG YRLPSPMDCP AALYQLMLDC WQKDRNSRPK
910 920 930 940 950
FDEIVSMLDK LIRNPSSLKT LVNASSRVSN LLVEHSPVGS GAYRSVGEWL
960 970 980 990 1000
EAIKMGRYTE IFMENGYSSM DSVAQVTLED LRRLGVTLVG HQKKIMNSLQ
1010
EMKVQLVNGM VPL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 981 – 1013 | LRRLG…GMVPL → ESPCEKWSLTLHPLFPTGYQ T in CAA79508 (PubMed:8510926).CuratedAdd BLAST | 33 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_003004 | 280 – 443 | Missing in isoform 2. CuratedAdd BLAST | 164 | |
Alternative sequenceiVSP_003005 | 573 – 595 | SCCDH…PSLIW → R in isoform 1 and isoform 2. CuratedAdd BLAST | 23 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03910 mRNA Translation: AAB60613.1 U03910 mRNA Translation: AAB60614.1 U03910 mRNA Translation: AAB60612.1 Z19058 mRNA Translation: CAA79508.1 |
PIRi | I50615 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U03910 mRNA Translation: AAB60613.1 U03910 mRNA Translation: AAB60614.1 U03910 mRNA Translation: AAB60612.1 Z19058 mRNA Translation: CAA79508.1 |
PIRi | I50615 |
3D structure databases
SMRi | P54755 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000037020 |
Proteomic databases
PaxDbi | P54755 |
Phylogenomic databases
eggNOGi | KOG0196, Eukaryota |
InParanoidi | P54755 |
OMAi | ECGPNVR |
OrthoDBi | 933071at2759 |
PhylomeDBi | P54755 |
Enzyme and pathway databases
BRENDAi | 2.7.10.1, 1306 |
Family and domain databases
CDDi | cd00063, FN3, 2 hits |
Gene3Di | 1.10.150.50, 1 hit 2.60.120.260, 1 hit 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR027936, Eph_TM IPR001090, Ephrin_rcpt_lig-bd_dom IPR003961, FN3_dom IPR036116, FN3_sf IPR008979, Galactose-bd-like_sf IPR009030, Growth_fac_rcpt_cys_sf IPR013783, Ig-like_fold IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001660, SAM IPR013761, SAM/pointed_sf IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008266, Tyr_kinase_AS IPR020635, Tyr_kinase_cat_dom IPR016257, Tyr_kinase_ephrin_rcpt IPR001426, Tyr_kinase_rcpt_V_CS |
Pfami | View protein in Pfam PF14575, EphA2_TM, 1 hit PF01404, Ephrin_lbd, 1 hit PF00041, fn3, 2 hits PF07714, PK_Tyr_Ser-Thr, 1 hit PF00536, SAM_1, 1 hit |
PIRSFi | PIRSF000666, TyrPK_ephrin_receptor, 1 hit |
PRINTSi | PR00109, TYRKINASE |
SMARTi | View protein in SMART SM00615, EPH_lbd, 1 hit SM00060, FN3, 2 hits SM00454, SAM, 1 hit SM00219, TyrKc, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF49265, SSF49265, 1 hit SSF49785, SSF49785, 1 hit SSF56112, SSF56112, 1 hit SSF57184, SSF57184, 1 hit |
PROSITEi | View protein in PROSITE PS01186, EGF_2, 1 hit PS51550, EPH_LBD, 1 hit PS50853, FN3, 2 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00109, PROTEIN_KINASE_TYR, 1 hit PS00790, RECEPTOR_TYR_KIN_V_1, 1 hit PS00791, RECEPTOR_TYR_KIN_V_2, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EPHA5_CHICK | |
Accessioni | P54755Primary (citable) accession number: P54755 Secondary accession number(s): Q07495 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families