UniProtKB - P54746 (MNGB_ECOLI)
Protein
Mannosylglycerate hydrolase
Gene
mngB
Organism
Escherichia coli (strain K12)
Status
Functioni
May hydrolyze 6-phospho-mannosyl-D-glycerate to mannose-6-phosphate and glycerate.1 Publication
Catalytic activityi
- (2R)-2-O-(6-phospho-α-D-mannosyl)-glycerate + H2O = (R)-glycerate + α-D-mannose 6-phosphate1 Publication
Cofactori
a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 14 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 16 | Divalent metal cationBy similarity | 1 | |
Active sitei | 126 | NucleophileBy similarity | 1 | |
Metal bindingi | 126 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 338 | Divalent metal cationBy similarity | 1 |
GO - Molecular functioni
- alpha-mannosidase activity Source: EcoCyc
- carbohydrate binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- mannose metabolic process Source: InterPro
- oligosaccharide catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG13236-MONOMER MetaCyc:EG13236-MONOMER |
Protein family/group databases
CAZyi | GH38, Glycoside Hydrolase Family 38 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosylglycerate hydrolase (EC:3.2.1.-1 Publication)Alternative name(s): 2-O-(6-phospho-mannosyl)-D-glycerate hydrolase Alpha-mannosidase mngB |
Gene namesi | Name:mngB Synonyms:ybgG Ordered Locus Names:b0732, JW0721 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000168700 | 1 – 877 | Mannosylglycerate hydrolaseAdd BLAST | 877 |
Proteomic databases
PaxDbi | P54746 |
PRIDEi | P54746 |
Expressioni
Inductioni
Repressed by MngR. Induced by mannosyl-D-glycerate.
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263542, 13 interactors |
IntActi | P54746, 14 interactors |
STRINGi | 511145.b0732 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 38 family.Curated
Phylogenomic databases
eggNOGi | COG0383, Bacteria |
HOGENOMi | CLU_003442_2_1_6 |
InParanoidi | P54746 |
PhylomeDBi | P54746 |
Family and domain databases
Gene3Di | 1.20.1270.50, 1 hit 3.20.110.10, 1 hit |
InterProi | View protein in InterPro IPR011013, Gal_mutarotase_sf_dom IPR011330, Glyco_hydro/deAcase_b/a-brl IPR011682, Glyco_hydro_38_C IPR015341, Glyco_hydro_38_cen IPR037094, Glyco_hydro_38_cen_sf IPR000602, Glyco_hydro_38_N IPR027291, Glyco_hydro_38_N_sf IPR028995, Glyco_hydro_57/38_cen_sf |
Pfami | View protein in Pfam PF09261, Alpha-mann_mid, 1 hit PF07748, Glyco_hydro_38C, 1 hit PF01074, Glyco_hydro_38N, 1 hit |
SMARTi | View protein in SMART SM00872, Alpha-mann_mid, 1 hit |
SUPFAMi | SSF74650, SSF74650, 1 hit SSF88688, SSF88688, 1 hit SSF88713, SSF88713, 1 hit |
i Sequence
Sequence statusi: Complete.
P54746-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAVSRVHIT PHMHWDREWY FTTEESRILL VNNMEEILCR LEQDNEYKYY
60 70 80 90 100
VLDGQTAILE DYFAVKPENK DRVKKQVEAG KLIIGPWYTQ TDTTIVSAES
110 120 130 140 150
IVRNLMYGMR DCLAFGEPMK IGYLPDSFGM SGQLPHIYNG FGITRTMFWR
160 170 180 190 200
GCSERHGTDK TEFLWQSSDG SEVTAQVLPL GYAIGKYLPA DENGLRKRLD
210 220 230 240 250
SYFDVLEKAS VTKEILLPNG HDQMPLQQNI FEVMDKLREI YPQRKFVMSR
260 270 280 290 300
FEEVFEKIEA QRDNLATLKG EFIDGKYMRV HRTIGSTRMD IKIAHARIEN
310 320 330 340 350
KIVNLLEPLA TLAWTLGFEY HHGLLEKMWK EILKNHAHDS IGCCCSDKVH
360 370 380 390 400
REIVARFELA EDMADNLIRF YMRKIADNMP QSDADKLVLF NLMPWPREEV
410 420 430 440 450
INTTVRLRAS QFNLRDDRGQ PVPYFIRHAR EIDPGLIDRQ IVHYGNYDPF
460 470 480 490 500
MEFDIQINQI VPSMGYRTLY IEANQPGNVI AAKSDAEGIL ENAFWQIALN
510 520 530 540 550
EDGSLQLVDK DSGVRYDRVL QIEESSDDGD EYDYSPAKEE WVITAANAKP
560 570 580 590 600
QCDIIHEAWQ SRAVIRYDMA VPLNLSERSA RQSTGRVGVV LVVTLSHNSR
610 620 630 640 650
RIDVDINLDN QADDHRLRVL VPTPFNTDSV LADTQFGSLT RPVNDSAMNN
660 670 680 690 700
WQQEGWKEAP VPVWNMLNYV ALQEGRNGMA VFSEGLREFE VIGEEKKTFA
710 720 730 740 750
ITLLRGVGLL GKEDLLLRPG RPSGIKMPVP DSQLRGLLSC RLSLLSYTGT
760 770 780 790 800
PTAAGVAQQA RAWLTPVQCY NKIPWDVMKL NKAGFNVPES YSLLKMPPVG
810 820 830 840 850
CLISALKKAE DRQEVILRLF NPAESATCDA TVAFSREVIS CSETMMDEHI
860 870
TTEENQGSNL SGPFLPGQSR TFSYRLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 – 100 | WYTQT…VSAES → GIPRPIPRLFCGNP (PubMed:9063979).CuratedAdd BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73826.1 AP009048 Genomic DNA Translation: BAA35398.1 D64014 Genomic DNA No translation available. |
PIRi | C64809 |
RefSeqi | NP_415260.1, NC_000913.3 WP_000648976.1, NZ_SSZK01000033.1 |
Genome annotation databases
EnsemblBacteriai | AAC73826; AAC73826; b0732 BAA35398; BAA35398; BAA35398 |
GeneIDi | 945359 |
KEGGi | ecj:JW0721 eco:b0732 |
PATRICi | fig|1411691.4.peg.1541 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC73826.1 AP009048 Genomic DNA Translation: BAA35398.1 D64014 Genomic DNA No translation available. |
PIRi | C64809 |
RefSeqi | NP_415260.1, NC_000913.3 WP_000648976.1, NZ_SSZK01000033.1 |
3D structure databases
SMRi | P54746 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263542, 13 interactors |
IntActi | P54746, 14 interactors |
STRINGi | 511145.b0732 |
Protein family/group databases
CAZyi | GH38, Glycoside Hydrolase Family 38 |
Proteomic databases
PaxDbi | P54746 |
PRIDEi | P54746 |
Genome annotation databases
EnsemblBacteriai | AAC73826; AAC73826; b0732 BAA35398; BAA35398; BAA35398 |
GeneIDi | 945359 |
KEGGi | ecj:JW0721 eco:b0732 |
PATRICi | fig|1411691.4.peg.1541 |
Organism-specific databases
EchoBASEi | EB3025 |
Phylogenomic databases
eggNOGi | COG0383, Bacteria |
HOGENOMi | CLU_003442_2_1_6 |
InParanoidi | P54746 |
PhylomeDBi | P54746 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG13236-MONOMER MetaCyc:EG13236-MONOMER |
Miscellaneous databases
PROi | PR:P54746 |
Family and domain databases
Gene3Di | 1.20.1270.50, 1 hit 3.20.110.10, 1 hit |
InterProi | View protein in InterPro IPR011013, Gal_mutarotase_sf_dom IPR011330, Glyco_hydro/deAcase_b/a-brl IPR011682, Glyco_hydro_38_C IPR015341, Glyco_hydro_38_cen IPR037094, Glyco_hydro_38_cen_sf IPR000602, Glyco_hydro_38_N IPR027291, Glyco_hydro_38_N_sf IPR028995, Glyco_hydro_57/38_cen_sf |
Pfami | View protein in Pfam PF09261, Alpha-mann_mid, 1 hit PF07748, Glyco_hydro_38C, 1 hit PF01074, Glyco_hydro_38N, 1 hit |
SMARTi | View protein in SMART SM00872, Alpha-mann_mid, 1 hit |
SUPFAMi | SSF74650, SSF74650, 1 hit SSF88688, SSF88688, 1 hit SSF88713, SSF88713, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MNGB_ECOLI | |
Accessioni | P54746Primary (citable) accession number: P54746 Secondary accession number(s): P75753 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | November 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries