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Protein

Mannosylglycerate hydrolase

Gene

mngB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May hydrolyze mannosyl-D-glycerate to mannose-6-phosphate and glycerate.1 Publication

Catalytic activityi

2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate + H2O = D-mannose 6-phosphate + D-glycerate.1 Publication

Cofactori

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14Divalent metal cationBy similarity1
Metal bindingi16Divalent metal cationBy similarity1
Active sitei126NucleophileBy similarity1
Metal bindingi126Divalent metal cationBy similarity1
Metal bindingi338Divalent metal cationBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: EcoliWiki
  • carbohydrate binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG13236-MONOMER
MetaCyc:EG13236-MONOMER

Protein family/group databases

CAZyiGH38 Glycoside Hydrolase Family 38

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosylglycerate hydrolase (EC:3.2.1.170)
Alternative name(s):
2-O-(6-phospho-mannosyl)-D-glycerate hydrolase
Alpha-mannosidase mngB
Gene namesi
Name:mngB
Synonyms:ybgG
Ordered Locus Names:b0732, JW0721
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13236 mngB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001687001 – 877Mannosylglycerate hydrolaseAdd BLAST877

Proteomic databases

PaxDbiP54746
PRIDEiP54746

Expressioni

Inductioni

Repressed by MngR. Induced by mannosyl-D-glycerate.

Interactioni

Protein-protein interaction databases

BioGridi4263542, 13 interactors
IntActiP54746, 14 interactors
STRINGi316385.ECDH10B_0798

Structurei

3D structure databases

ProteinModelPortaliP54746
SMRiP54746
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Phylogenomic databases

eggNOGiENOG4105C8I Bacteria
COG0383 LUCA
HOGENOMiHOG000231434
InParanoidiP54746
KOiK15524
OMAiNKPAASW
PhylomeDBiP54746

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

P54746-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVSRVHIT PHMHWDREWY FTTEESRILL VNNMEEILCR LEQDNEYKYY
60 70 80 90 100
VLDGQTAILE DYFAVKPENK DRVKKQVEAG KLIIGPWYTQ TDTTIVSAES
110 120 130 140 150
IVRNLMYGMR DCLAFGEPMK IGYLPDSFGM SGQLPHIYNG FGITRTMFWR
160 170 180 190 200
GCSERHGTDK TEFLWQSSDG SEVTAQVLPL GYAIGKYLPA DENGLRKRLD
210 220 230 240 250
SYFDVLEKAS VTKEILLPNG HDQMPLQQNI FEVMDKLREI YPQRKFVMSR
260 270 280 290 300
FEEVFEKIEA QRDNLATLKG EFIDGKYMRV HRTIGSTRMD IKIAHARIEN
310 320 330 340 350
KIVNLLEPLA TLAWTLGFEY HHGLLEKMWK EILKNHAHDS IGCCCSDKVH
360 370 380 390 400
REIVARFELA EDMADNLIRF YMRKIADNMP QSDADKLVLF NLMPWPREEV
410 420 430 440 450
INTTVRLRAS QFNLRDDRGQ PVPYFIRHAR EIDPGLIDRQ IVHYGNYDPF
460 470 480 490 500
MEFDIQINQI VPSMGYRTLY IEANQPGNVI AAKSDAEGIL ENAFWQIALN
510 520 530 540 550
EDGSLQLVDK DSGVRYDRVL QIEESSDDGD EYDYSPAKEE WVITAANAKP
560 570 580 590 600
QCDIIHEAWQ SRAVIRYDMA VPLNLSERSA RQSTGRVGVV LVVTLSHNSR
610 620 630 640 650
RIDVDINLDN QADDHRLRVL VPTPFNTDSV LADTQFGSLT RPVNDSAMNN
660 670 680 690 700
WQQEGWKEAP VPVWNMLNYV ALQEGRNGMA VFSEGLREFE VIGEEKKTFA
710 720 730 740 750
ITLLRGVGLL GKEDLLLRPG RPSGIKMPVP DSQLRGLLSC RLSLLSYTGT
760 770 780 790 800
PTAAGVAQQA RAWLTPVQCY NKIPWDVMKL NKAGFNVPES YSLLKMPPVG
810 820 830 840 850
CLISALKKAE DRQEVILRLF NPAESATCDA TVAFSREVIS CSETMMDEHI
860 870
TTEENQGSNL SGPFLPGQSR TFSYRLA
Length:877
Mass (Da):100,015
Last modified:November 1, 1997 - v2
Checksum:iB67201C6F8CB1C0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 100WYTQT…VSAES → GIPRPIPRLFCGNP (PubMed:9063979).CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73826.1
AP009048 Genomic DNA Translation: BAA35398.1
D64014 Genomic DNA No translation available.
PIRiC64809
RefSeqiNP_415260.1, NC_000913.3
WP_000648976.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73826; AAC73826; b0732
BAA35398; BAA35398; BAA35398
GeneIDi945359
KEGGiecj:JW0721
eco:b0732
PATRICifig|1411691.4.peg.1541

Similar proteinsi

Entry informationi

Entry nameiMNGB_ECOLI
AccessioniPrimary (citable) accession number: P54746
Secondary accession number(s): P75753
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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