mngB

Functionⁱ

May hydrolyze mannosyl-D-glycerate to mannose-6-phosphate and glycerate.1 Publication

Catalytic activityⁱ

2-O-(6-phospho-alpha-D-mannosyl)-D-glycerate + H₂O = D-mannose 6-phosphate + D-glycerate.1 Publication

Cofactorⁱ

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	14	Divalent metal cationBy similarity	1
Metal bindingⁱ	16	Divalent metal cationBy similarity	1
Active siteⁱ	126	NucleophileBy similarity	1
Metal bindingⁱ	126	Divalent metal cationBy similarity	1
Metal bindingⁱ	338	Divalent metal cationBy similarity	1

GO - Molecular functionⁱ

alpha-mannosidase activity
Source: EcoliWiki
Inferred from mutant phenotypeⁱ
- 14645248
carbohydrate binding Source: InterPro
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mannose metabolic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Ligand	Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG13236-MONOMER MetaCyc:EG13236-MONOMER

BioCycⁱ

EcoCyc:EG13236-MONOMER
MetaCyc:EG13236-MONOMER

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH38 Glycoside Hydrolase Family 38

CAZyⁱ

GH38 Glycoside Hydrolase Family 38

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Mannosylglycerate hydrolase (EC:3.2.1.170) Alternative name(s): 2-O-(6-phospho-mannosyl)-D-glycerate hydrolase Alpha-mannosidase mngB
Gene namesⁱ	Name:mngB Synonyms:ybgG Ordered Locus Names:b0732, JW0721
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG13236 mngB

EcoGeneⁱ

EG13236 mngB

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000168700	1 – 877	Mannosylglycerate hydrolaseAdd BLAST		877

Proteomic databases

PaxDbⁱ	P54746
PRIDEⁱ	P54746

Expressionⁱ

Inductionⁱ

Repressed by MngR. Induced by mannosyl-D-glycerate.

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4263542, 13 interactors
IntActⁱ	P54746, 14 interactors
STRINGⁱ	316385.ECDH10B_0798

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P54746
SMRⁱ	P54746
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 38 family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105C8I Bacteria COG0383 LUCA
HOGENOMⁱ	HOG000231434
InParanoidⁱ	P54746
KEGG Orthology (KO) More...KOⁱ	K15524
PhylomeDBⁱ	P54746

Family and domain databases

Gene3Dⁱ	1.20.1270.50, 1 hit 3.20.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR011013 Gal_mutarotase_sf_dom IPR011330 Glyco_hydro/deAcase_b/a-brl IPR011682 Glyco_hydro_38_C IPR015341 Glyco_hydro_38_cen IPR037094 Glyco_hydro_38_cen_sf IPR000602 Glyco_hydro_38_N IPR027291 Glyco_hydro_38_N_sf IPR028995 Glyco_hydro_57/38_cen_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF09261 Alpha-mann_mid, 1 hit PF01074 Glyco_hydro_38, 1 hit PF07748 Glyco_hydro_38C, 1 hit
SMARTⁱ	View protein in SMART SM00872 Alpha-mann_mid, 1 hit
SUPFAMⁱ	SSF74650 SSF74650, 1 hit SSF88688 SSF88688, 1 hit SSF88713 SSF88713, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P54746-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKAVSRVHIT PHMHWDREWY FTTEESRILL VNNMEEILCR LEQDNEYKYY 
        60         70         80         90        100
VLDGQTAILE DYFAVKPENK DRVKKQVEAG KLIIGPWYTQ TDTTIVSAES 
       110        120        130        140        150
IVRNLMYGMR DCLAFGEPMK IGYLPDSFGM SGQLPHIYNG FGITRTMFWR 
       160        170        180        190        200
GCSERHGTDK TEFLWQSSDG SEVTAQVLPL GYAIGKYLPA DENGLRKRLD 
       210        220        230        240        250
SYFDVLEKAS VTKEILLPNG HDQMPLQQNI FEVMDKLREI YPQRKFVMSR 
       260        270        280        290        300
FEEVFEKIEA QRDNLATLKG EFIDGKYMRV HRTIGSTRMD IKIAHARIEN 
       310        320        330        340        350
KIVNLLEPLA TLAWTLGFEY HHGLLEKMWK EILKNHAHDS IGCCCSDKVH 
       360        370        380        390        400
REIVARFELA EDMADNLIRF YMRKIADNMP QSDADKLVLF NLMPWPREEV 
       410        420        430        440        450
INTTVRLRAS QFNLRDDRGQ PVPYFIRHAR EIDPGLIDRQ IVHYGNYDPF 
       460        470        480        490        500
MEFDIQINQI VPSMGYRTLY IEANQPGNVI AAKSDAEGIL ENAFWQIALN 
       510        520        530        540        550
EDGSLQLVDK DSGVRYDRVL QIEESSDDGD EYDYSPAKEE WVITAANAKP 
       560        570        580        590        600
QCDIIHEAWQ SRAVIRYDMA VPLNLSERSA RQSTGRVGVV LVVTLSHNSR 
       610        620        630        640        650
RIDVDINLDN QADDHRLRVL VPTPFNTDSV LADTQFGSLT RPVNDSAMNN 
       660        670        680        690        700
WQQEGWKEAP VPVWNMLNYV ALQEGRNGMA VFSEGLREFE VIGEEKKTFA 
       710        720        730        740        750
ITLLRGVGLL GKEDLLLRPG RPSGIKMPVP DSQLRGLLSC RLSLLSYTGT 
       760        770        780        790        800
PTAAGVAQQA RAWLTPVQCY NKIPWDVMKL NKAGFNVPES YSLLKMPPVG 
       810        820        830        840        850
CLISALKKAE DRQEVILRLF NPAESATCDA TVAFSREVIS CSETMMDEHI 
       860        870 
TTEENQGSNL SGPFLPGQSR TFSYRLA

Length:877

Mass (Da):100,015

Last modified:November 1, 1997 - v2

Checksum:ⁱB67201C6F8CB1C0A

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	87 – 100	WYTQT…VSAES → GIPRPIPRLFCGNP (PubMed:9063979).CuratedAdd BLAST		14

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73826.1 AP009048 Genomic DNA Translation: BAA35398.1 D64014 Genomic DNA No translation available.
PIRⁱ	C64809
NCBI Reference Sequences More...RefSeqⁱ	NP_415260.1, NC_000913.3 WP_000648976.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73826; AAC73826; b0732 BAA35398; BAA35398; BAA35398
GeneIDⁱ	945359
KEGGⁱ	ecj:JW0721 eco:b0732
PATRICⁱ	fig\|1411691.4.peg.1541

Protein	Similar proteins		Species	Score	Length	Source
P54746	Mannosylglycerate hydrolase		Shigella sp.		877	UniRef100_P54746
Alpha-mannosidase		ECOLX		877
Alpha-mannosidase mngB		Escherichia coli HVH 50 (4-2593475)		877
Mannosylglycerate hydrolase		SHIDY		877
Alpha-mannosidase MngB		Escherichia coli UMNK88		877
+18

Protein	Similar proteins		Species	Score	Length	Source
P54746	Mannosylglycerate hydrolase		Shigella sp.		877	UniRef90_P54746
Alpha-mannosidase		ECOLX		877
Alpha-mannosidase mngB		Escherichia coli HVH 50 (4-2593475)		877
Mannosylglycerate hydrolase		SHIDY		877
Alpha-mannosidase MngB		Escherichia coli UMNK88		877
+825

Protein	Similar proteins		Species	Score	Length	Source
P54746	Mannosylglycerate hydrolase		Shigella sp.		877	UniRef50_P54746
Alpha-mannosidase		ECOLX		877
Alpha-mannosidase mngB		Escherichia coli HVH 50 (4-2593475)		877
Mannosylglycerate hydrolase		SHIDY		877
Alpha-mannosidase MngB		Escherichia coli UMNK88		877
+1715

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC73826.1 AP009048 Genomic DNA Translation: BAA35398.1 D64014 Genomic DNA No translation available.
PIRⁱ	C64809
RefSeqⁱ	NP_415260.1, NC_000913.3 WP_000648976.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P54746
SMRⁱ	P54746
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263542, 13 interactors
IntActⁱ	P54746, 14 interactors
STRINGⁱ	316385.ECDH10B_0798

Protein family/group databases

CAZyⁱ	GH38 Glycoside Hydrolase Family 38

CAZyⁱ

GH38 Glycoside Hydrolase Family 38

Proteomic databases

PaxDbⁱ	P54746
PRIDEⁱ	P54746

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73826; AAC73826; b0732 BAA35398; BAA35398; BAA35398
GeneIDⁱ	945359
KEGGⁱ	ecj:JW0721 eco:b0732
PATRICⁱ	fig\|1411691.4.peg.1541

Organism-specific databases

EchoBASEⁱ	EB3025
EcoGeneⁱ	EG13236 mngB

Phylogenomic databases

eggNOGⁱ	ENOG4105C8I Bacteria COG0383 LUCA
HOGENOMⁱ	HOG000231434
InParanoidⁱ	P54746
KOⁱ	K15524
PhylomeDBⁱ	P54746

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG13236-MONOMER MetaCyc:EG13236-MONOMER

BioCycⁱ

EcoCyc:EG13236-MONOMER
MetaCyc:EG13236-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P54746

PROⁱ

PR:P54746

Family and domain databases

Gene3Dⁱ	1.20.1270.50, 1 hit 3.20.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR011013 Gal_mutarotase_sf_dom IPR011330 Glyco_hydro/deAcase_b/a-brl IPR011682 Glyco_hydro_38_C IPR015341 Glyco_hydro_38_cen IPR037094 Glyco_hydro_38_cen_sf IPR000602 Glyco_hydro_38_N IPR027291 Glyco_hydro_38_N_sf IPR028995 Glyco_hydro_57/38_cen_sf
Pfamⁱ	View protein in Pfam PF09261 Alpha-mann_mid, 1 hit PF01074 Glyco_hydro_38, 1 hit PF07748 Glyco_hydro_38C, 1 hit
SMARTⁱ	View protein in SMART SM00872 Alpha-mann_mid, 1 hit
SUPFAMⁱ	SSF74650 SSF74650, 1 hit SSF88688 SSF88688, 1 hit SSF88713 SSF88713, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MNGB_ECOLI
Accessionⁱ	P54746Primary (citable) accession number: P54746 Secondary accession number(s): P75753
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
	Last sequence update:	November 1, 1997
	Last modified:	November 7, 2018
	This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

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UniProtKB - P54746 (MNGB_ECOLI)

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Mannosylglycerate hydrolase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Molecule processing

Proteomic databases

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<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

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Experimental Info

Sequence databases

Genome annotation databases

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3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

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Family and domain databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ