UniProtKB - P54745 (MNGA_ECOLI)
Protein
PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component
Gene
mngA
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane (PubMed:14645248, PubMed:9063979). This system is involved in mannosyl-D-glycerate transport (PubMed:14645248). Also involved in thermoinduction of ompC (PubMed:9063979).2 Publications
Catalytic activityi
- (2R)-2-O-(α-D-mannosyl)-glycerate(out) + Nπ-phospho-L-histidyl-[protein] = (2R)-2-O-(6-phospho-α-D-mannosyl)-glycerate(in) + L-histidyl-[protein]1 PublicationEC:2.7.1.1951 Publication
Kineticsi
- KM=10 µM for mannosyl-D-glycerate1 Publication
- Vmax=0.65 nmol/min/mg enzyme for mannosyl-D-glycerate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 87 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 192 | Phosphocysteine intermediate; for EIIB activity1 Publication | 1 |
GO - Molecular functioni
- carbohydrate:proton symporter activity Source: InterPro
- kinase activity Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity Source: InterPro
- protein-phosphocysteine-mannosylglycerate-phosphotransferase system transporter activity Source: EcoCyc
- protein-phosphocysteine-sugar phosphotransferase activity Source: EcoCyc
GO - Biological processi
- mannosylglycerate transport Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:HRSA-MONOMER MetaCyc:HRSA-MONOMER |
Protein family/group databases
TCDBi | 4.A.2.1.3, the pts fructose-mannitol (fru) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component1 PublicationAlternative name(s): 2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme MngA1 Publication Protein-Npi-phosphohistidine--2-O-alpha-mannosyl-D-glycerate phosphotransferase1 Publication Including the following 3 domains: 2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme IIA component1 Publication Alternative name(s): PTS system EIIA component1 Publication 2-O-alpha-mannosyl-D-glycerate-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.1951 Publication) Alternative name(s): PTS system EIIB component1 Publication 2-O-alpha-mannosyl-D-glycerate-specific permease IIC component1 Publication Alternative name(s): PTS system EIIC component1 Publication |
Gene namesi | Name:mngA1 Publication Synonyms:hrsA1 Publication Ordered Locus Names:b0731, JW0720 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane PROSITE-ProRule annotation1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 313 | PeriplasmicSequence analysisAdd BLAST | 313 | |
Transmembranei | 314 – 334 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 335 – 358 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 359 – 379 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 380 – 389 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 390 – 410 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 411 – 433 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 434 – 454 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 455 – 474 | PeriplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 475 – 495 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 496 – 500 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 501 – 521 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 522 – 551 | PeriplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 552 – 572 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 573 | CytoplasmicSequence analysis | 1 | |
Transmembranei | 574 – 594 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 595 – 620 | PeriplasmicSequence analysisAdd BLAST | 26 | |
Transmembranei | 621 – 641 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 642 – 658 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene are unable to transport mannosyl-D-glycerate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186699 | 1 – 658 | PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC componentAdd BLAST | 658 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 87 | Phosphohistidine; by HPrCurated | 1 | |
Modified residuei | 192 | Phosphocysteine; by EIIAPROSITE-ProRule annotationCurated | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P54745 |
PRIDEi | P54745 |
Expressioni
Inductioni
Induced by mannosyl-D-glycerate. Repressed by MngR.1 Publication
Interactioni
Binary interactionsi
P54745
With | #Exp. | IntAct |
---|---|---|
sspA [P0ACA3] | 5 | EBI-558542,EBI-558482 |
Protein-protein interaction databases
BioGRIDi | 4263541, 15 interactors 849731, 1 interactor |
DIPi | DIP-9939N |
IntActi | P54745, 1 interactor |
STRINGi | 511145.b0731 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 171 | PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST | 147 | |
Domaini | 186 – 282 | PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST | 97 | |
Domaini | 306 – 641 | PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST | 336 |
Domaini
The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1299, Bacteria COG1445, Bacteria COG1762, Bacteria |
HOGENOMi | CLU_013155_1_2_6 |
PhylomeDBi | P54745 |
Family and domain databases
CDDi | cd00211, PTS_IIA_fru, 1 hit cd05569, PTS_IIB_fructose, 1 hit |
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR016152, PTrfase/Anion_transptr IPR002178, PTS_EIIA_type-2_dom IPR036095, PTS_EIIB-like_sf IPR013011, PTS_EIIB_2 IPR003501, PTS_EIIB_2/3 IPR003352, PTS_EIIC IPR013014, PTS_EIIC_2 IPR004715, PTS_IIA_fruc IPR003353, PTS_IIB_fruc IPR006327, PTS_IIC_fruc |
Pfami | View protein in Pfam PF00359, PTS_EIIA_2, 1 hit PF02378, PTS_EIIC, 1 hit PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00829, FRU, 1 hit TIGR00848, fruA, 1 hit TIGR01427, PTS_IIC_fructo, 1 hit |
PROSITEi | View protein in PROSITE PS51094, PTS_EIIA_TYPE_2, 1 hit PS00372, PTS_EIIA_TYPE_2_HIS, 1 hit PS51099, PTS_EIIB_TYPE_2, 1 hit PS51104, PTS_EIIC_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P54745-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVLFYRAHWR DYKNDQVRIM MNLTTLTHRD ALCLNARFTS REEAIHALTQ
60 70 80 90 100
RLAALGKISS TEQFLEEVYR RESLGPTALG EGLAVPHGKT AAVKEAAFAV
110 120 130 140 150
ATLSEPLQWE GVDGPEAVDL VVLLAIPPNE AGTTHMQLLT ALTTRLADDE
160 170 180 190 200
IRARIQSATT PDELLSALDD KGGTQPSASF SNAPTIVCVT ACPAGIAHTY
210 220 230 240 250
MAAEYLEKAG RKLGVNVYVE KQGANGIEGR LTADQLNSAT ACIFAAEVAI
260 270 280 290 300
KESERFNGIP ALSVPVAEPI RHAEALIQQA LTLKRSDETR TVQQDTQPVK
310 320 330 340 350
SVKTELKQAL LSGISFAVPL IVAGGTVLAV AVLLSQIFGL QDLFNEENSW
360 370 380 390 400
LWMYRKLGGG LLGILMVPVL AAYTAYSLAD KPALAPGFAA GLAANMIGSG
410 420 430 440 450
FLGAVVGGLI AGYLMRWVKN HLRLSSKFNG FLTFYLYPVL GTLGAGSLML
460 470 480 490 500
FVVGEPVAWI NNSLTAWLNG LSGSNALLLG AILGFMCSFD LGGPVNKAAY
510 520 530 540 550
AFCLGAMANG VYGPYAIFAS VKMVSAFTVT ASTMLAPRLF KEFEIETGKS
560 570 580 590 600
TWLLGLAGIT EGAIPMAIED PLRVIGSFVL GSMVTGAIVG AMNIGLSTPG
610 620 630 640 650
AGIFSLFLLH DNGAGGVMAA IGWFGAALVG AAISTAILLM WRRHAVKHGN
YLTDGVMP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64014 Genomic DNA Translation: BAA10893.1 U00096 Genomic DNA Translation: AAC73825.1 AP009048 Genomic DNA Translation: BAA35397.2 |
PIRi | JC4598 |
RefSeqi | NP_415259.1, NC_000913.3 WP_000242633.1, NZ_CP047127.1 |
Genome annotation databases
EnsemblBacteriai | AAC73825; AAC73825; b0731 BAA35397; BAA35397; BAA35397 |
GeneIDi | 945355 |
KEGGi | ecj:JW0720 eco:b0731 |
PATRICi | fig|511145.12.peg.761 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D64014 Genomic DNA Translation: BAA10893.1 U00096 Genomic DNA Translation: AAC73825.1 AP009048 Genomic DNA Translation: BAA35397.2 |
PIRi | JC4598 |
RefSeqi | NP_415259.1, NC_000913.3 WP_000242633.1, NZ_CP047127.1 |
3D structure databases
SMRi | P54745 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263541, 15 interactors 849731, 1 interactor |
DIPi | DIP-9939N |
IntActi | P54745, 1 interactor |
STRINGi | 511145.b0731 |
Protein family/group databases
TCDBi | 4.A.2.1.3, the pts fructose-mannitol (fru) family |
Proteomic databases
PaxDbi | P54745 |
PRIDEi | P54745 |
Genome annotation databases
EnsemblBacteriai | AAC73825; AAC73825; b0731 BAA35397; BAA35397; BAA35397 |
GeneIDi | 945355 |
KEGGi | ecj:JW0720 eco:b0731 |
PATRICi | fig|511145.12.peg.761 |
Organism-specific databases
EchoBASEi | EB3024 |
Phylogenomic databases
eggNOGi | COG1299, Bacteria COG1445, Bacteria COG1762, Bacteria |
HOGENOMi | CLU_013155_1_2_6 |
PhylomeDBi | P54745 |
Enzyme and pathway databases
BioCyci | EcoCyc:HRSA-MONOMER MetaCyc:HRSA-MONOMER |
Miscellaneous databases
PROi | PR:P54745 |
Family and domain databases
CDDi | cd00211, PTS_IIA_fru, 1 hit cd05569, PTS_IIB_fructose, 1 hit |
Gene3Di | 3.40.930.10, 1 hit |
InterProi | View protein in InterPro IPR016152, PTrfase/Anion_transptr IPR002178, PTS_EIIA_type-2_dom IPR036095, PTS_EIIB-like_sf IPR013011, PTS_EIIB_2 IPR003501, PTS_EIIB_2/3 IPR003352, PTS_EIIC IPR013014, PTS_EIIC_2 IPR004715, PTS_IIA_fruc IPR003353, PTS_IIB_fruc IPR006327, PTS_IIC_fruc |
Pfami | View protein in Pfam PF00359, PTS_EIIA_2, 1 hit PF02378, PTS_EIIC, 1 hit PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit SSF55804, SSF55804, 1 hit |
TIGRFAMsi | TIGR00829, FRU, 1 hit TIGR00848, fruA, 1 hit TIGR01427, PTS_IIC_fructo, 1 hit |
PROSITEi | View protein in PROSITE PS51094, PTS_EIIA_TYPE_2, 1 hit PS00372, PTS_EIIA_TYPE_2_HIS, 1 hit PS51099, PTS_EIIB_TYPE_2, 1 hit PS51104, PTS_EIIC_TYPE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MNGA_ECOLI | |
Accessioni | P54745Primary (citable) accession number: P54745 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | April 7, 2021 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |