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Protein

NEDD8 ultimate buster 1

Gene

Nub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation (By similarity).By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8 ultimate buster 1
Alternative name(s):
Negative regulator of ubiquitin-like proteins 1
Protein BS4
Gene namesi
Name:Nub1
Synonyms:Nyren18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1889001 Nub1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109931 – 614NEDD8 ultimate buster 1Add BLAST614

Proteomic databases

EPDiP54729
PaxDbiP54729
PeptideAtlasiP54729
PRIDEiP54729

PTM databases

iPTMnetiP54729
PhosphoSitePlusiP54729

Expressioni

Developmental stagei

Strongest expression at 7 dpc. Marked decrease 11, 15 and 17 dpc.

Inductioni

By interferon.

Gene expression databases

BgeeiENSMUSG00000028954 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_NUB1
ExpressionAtlasiP54729 baseline and differential
GenevisibleiP54729 MM

Interactioni

Subunit structurei

Directly interacts with NEDD8 and PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome. Interacts with AIPL1. The interaction with UBD via UBA domains facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates UBD degradation and that of its conjugates (By similarity).By similarity

Protein-protein interaction databases

BioGridi207276, 2 interactors
STRINGi10090.ENSMUSP00000070265

Structurei

Secondary structure

1614
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP54729
SMRiP54729
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54729

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 413UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini423 – 469UBA 2PROSITE-ProRule annotationAdd BLAST47
Domaini488 – 528UBA 3PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni426 – 473NEDD8-binding 1By similarityAdd BLAST48
Regioni549 – 597NEDD8-binding 2By similarityAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili36 – 71Sequence analysisAdd BLAST36
Coiled coili151 – 206Sequence analysisAdd BLAST56

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi413 – 430Nuclear localization signalCuratedAdd BLAST18

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG2561 Eukaryota
ENOG410ZK3P LUCA
GeneTreeiENSGT00390000010557
HOGENOMiHOG000113882
HOVERGENiHBG045600
InParanoidiP54729
PhylomeDBiP54729
TreeFamiTF323449

Family and domain databases

InterProiView protein in InterPro
IPR039749 NUB1
IPR011990 TPR-like_helical_dom_sf
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12948 PTHR12948, 1 hit
PfamiView protein in Pfam
PF00627 UBA, 3 hits
SMARTiView protein in SMART
SM00165 UBA, 3 hits
SUPFAMiSSF46934 SSF46934, 3 hits
SSF48452 SSF48452, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P54729-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQKKYLQAK LTQFLREDRI QLWKPPYTKE NKEVGLAVKD LAKKYSERLE
60 70 80 90 100
CCENEVENII EEIRRKAIER GTGNEHYRTT GIATIEVFLP PRLRKHDKKS
110 120 130 140 150
LLETRLHVTG RDLRCQIAET FGFQENYIKI VINKKQLQLG KSLEEQGVTH
160 170 180 190 200
NVKAMVLELK QSEEDVRKNL QLEEEEQNEA ELKERRIQRT KRGLEILAER
210 220 230 240 250
AEMVVDPETM PYLDIANQTG RSLRIPPAER KALMLAMGYH EKGRAFLKRK
260 270 280 290 300
EYGIALPCLL DADRYFCECK ELLDTVDNYA VLQLDIVWCY FRLEQLECLD
310 320 330 340 350
DAEKKLNLAQ KCFKNCYGEN HQRLVHIKGN CGKEKVLFLR LYLLQGIQNY
360 370 380 390 400
HSGNGEEARE YLNKARQLFK ELYIDPSKVH NLLQLGFTAQ EARLGLRACD
410 420 430 440 450
GNVDHAATHI SNRREELAQI RKEEKEKRRR RLENVNTLRG MGYSTQAAKQ
460 470 480 490 500
ALHQARGNLD DALKVLLSNP HMWWLQDADP ENNSRQASPS QESINQLVYM
510 520 530 540 550
GFDTVVAEAA LRVFGGNVQL AAQTLAHHGG SLPPDLQFSG EDSSPTPSTS
560 570 580 590 600
PSDSAGTSSA STDEDMETEA VNEILEDIPE HEEDYLDSTL EDEEVIIAEY
610
LSYVESISSA AKNN
Length:614
Mass (Da):70,307
Last modified:September 26, 2003 - v2
Checksum:i8287222F141D1B80
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JGQ4A0A0G2JGQ4_MOUSE
NEDD8 ultimate buster 1
Nub1 6330412F12Rik, mCG_16508
638Annotation score:

Sequence cautioni

The sequence AAA68612 differs from that shown. Reason: Frameshift at position 611.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27462 mRNA Translation: AAA68612.1 Frameshift.
AF534114 mRNA Translation: AAM97686.1
BC037451 mRNA Translation: AAH37451.1
CCDSiCCDS39030.1
RefSeqiNP_058016.2, NM_016736.3
UniGeneiMm.407975
Mm.5856

Genome annotation databases

EnsembliENSMUST00000068825; ENSMUSP00000070265; ENSMUSG00000028954
GeneIDi53312
KEGGimmu:53312
UCSCiuc008wsf.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27462 mRNA Translation: AAA68612.1 Frameshift.
AF534114 mRNA Translation: AAM97686.1
BC037451 mRNA Translation: AAH37451.1
CCDSiCCDS39030.1
RefSeqiNP_058016.2, NM_016736.3
UniGeneiMm.407975
Mm.5856

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VEGNMR-A469-538[»]
ProteinModelPortaliP54729
SMRiP54729
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207276, 2 interactors
STRINGi10090.ENSMUSP00000070265

PTM databases

iPTMnetiP54729
PhosphoSitePlusiP54729

Proteomic databases

EPDiP54729
PaxDbiP54729
PeptideAtlasiP54729
PRIDEiP54729

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000068825; ENSMUSP00000070265; ENSMUSG00000028954
GeneIDi53312
KEGGimmu:53312
UCSCiuc008wsf.2 mouse

Organism-specific databases

CTDi51667
MGIiMGI:1889001 Nub1

Phylogenomic databases

eggNOGiKOG2561 Eukaryota
ENOG410ZK3P LUCA
GeneTreeiENSGT00390000010557
HOGENOMiHOG000113882
HOVERGENiHBG045600
InParanoidiP54729
PhylomeDBiP54729
TreeFamiTF323449

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation

Miscellaneous databases

ChiTaRSiNub1 mouse
EvolutionaryTraceiP54729
PROiPR:P54729
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028954 Expressed in 292 organ(s), highest expression level in embryo
CleanExiMM_NUB1
ExpressionAtlasiP54729 baseline and differential
GenevisibleiP54729 MM

Family and domain databases

InterProiView protein in InterPro
IPR039749 NUB1
IPR011990 TPR-like_helical_dom_sf
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR12948 PTHR12948, 1 hit
PfamiView protein in Pfam
PF00627 UBA, 3 hits
SMARTiView protein in SMART
SM00165 UBA, 3 hits
SUPFAMiSSF46934 SSF46934, 3 hits
SSF48452 SSF48452, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNUB1_MOUSE
AccessioniPrimary (citable) accession number: P54729
Secondary accession number(s): Q8K3U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 26, 2003
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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