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Protein

UV excision repair protein RAD23 homolog A

Gene

RAD23A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
(Microbial infection) Involved in Vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 Vpr with the host proteasome.1 Publication

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • kinase binding Source: CAFA
  • polyubiquitin modification-dependent protein binding Source: UniProtKB
  • single-stranded DNA binding Source: ProtInc
  • ubiquitin-specific protease binding Source: ParkinsonsUK-UCL

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Host-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-5689877 Josephin domain DUBs
R-HSA-5696394 DNA Damage Recognition in GG-NER
R-HSA-5696395 Formation of Incision Complex in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23 homolog A
Short name:
HR23A
Short name:
hHR23A
Gene namesi
Name:RAD23A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000179262.9
HGNCiHGNC:9812 RAD23A
MIMi600061 gene
neXtProtiNX_P54725

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi8K → A: No effect on interaction with EEF1A1. 1 Publication1
Mutagenesisi9T → A: Abolishes interaction with PSMD4; when associated with T-49. 1 Publication1
Mutagenesisi10L → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-47. 1 Publication1
Mutagenesisi47K → A: No effect on UBL-UBA domain interaction. 1 Publication1
Mutagenesisi47K → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-10. 1 Publication1
Mutagenesisi47K → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-77. 1 Publication1
Mutagenesisi49I → A: Impairs interaction with PSMD4. 2 Publications1
Mutagenesisi49I → T: Abolishes interaction with PSMD4; when associated with A-9. 2 Publications1
Mutagenesisi54I → A: Impairs interaction with PSMD4. 1 Publication1
Mutagenesisi71F → A: Impairs interaction with PSMD4. 1 Publication1
Mutagenesisi77T → E: Impairs UBL-UBA domain interaction and enhances ubiquitin-binding; when associated with Glu-47. 2 Publications1
Mutagenesisi77T → S: No effect on interaction with PSMD4. 2 Publications1
Mutagenesisi79T → P: Increases interaction with PSMD1 and PSMC1. 1 Publication1
Mutagenesisi333P → E: Abolishes interaction with HIV-1 vpr. 1 Publication1

Organism-specific databases

DisGeNETi5886
OpenTargetsiENSG00000179262
PharmGKBiPA34172

Polymorphism and mutation databases

BioMutaiRAD23A
DMDMi1709983

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149041 – 363UV excision repair protein RAD23 homolog AAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residuei123PhosphoserineCombined sources1
Modified residuei128PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineBy similarity1
Modified residuei205PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei357PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP54725
MaxQBiP54725
PaxDbiP54725
PeptideAtlasiP54725
PRIDEiP54725
ProteomicsDBi56700

PTM databases

iPTMnetiP54725
PhosphoSitePlusiP54725

Miscellaneous databases

PMAP-CutDBiP54725

Expressioni

Gene expression databases

BgeeiENSG00000179262 Expressed in 234 organ(s), highest expression level in muscle of leg
CleanExiHS_RAD23A
ExpressionAtlasiP54725 baseline and differential
GenevisibleiP54725 HS

Organism-specific databases

HPAiHPA026418
HPA053901
HPA057003
HPA065599

Interactioni

Subunit structurei

Interacts with XPC; the interaction is suggesting the existence of a functional equivalent variant XPC complex. Interacts with PSMD4 and PSMC5. Interacts with ATXN3. Interacts with UBQLN2.6 Publications
(Microbial infection) Interacts with HIV-1 Vpr.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111823, 246 interactors
DIPiDIP-34442N
IntActiP54725, 73 interactors
MINTiP54725
STRINGi9606.ENSP00000467024

Structurei

Secondary structure

1363
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00156
ProteinModelPortaliP54725
SMRiP54725
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP54725

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 81Ubiquitin-likePROSITE-ProRule annotationAdd BLAST81
Domaini161 – 201UBA 1PROSITE-ProRule annotationAdd BLAST41
Domaini318 – 358UBA 2PROSITE-ProRule annotationAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni319 – 363HIV-1 vpr bindingAdd BLAST45

Domaini

The ubiquitin-like domain mediates interaction with ATXN3.
The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.

Sequence similaritiesi

Belongs to the RAD23 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0011 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00390000012078
HOGENOMiHOG000172162
HOVERGENiHBG055042
InParanoidiP54725
KOiK10839
OMAiKGRDAFP
OrthoDBiEOG091G0DVL
PhylomeDBiP54725

Family and domain databases

Gene3Di1.10.10.540, 1 hit
InterProiView protein in InterPro
IPR004806 Rad23
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR015360 XPC-bd
IPR036353 XPC-bd_sf
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00240 ubiquitin, 1 hit
PF09280 XPC-binding, 1 hit
PRINTSiPR01839 RAD23PROTEIN
SMARTiView protein in SMART
SM00727 STI1, 1 hit
SM00165 UBA, 2 hits
SM00213 UBQ, 1 hit
SUPFAMiSSF101238 SSF101238, 1 hit
SSF46934 SSF46934, 2 hits
SSF54236 SSF54236, 1 hit
TIGRFAMsiTIGR00601 rad23, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS50053 UBIQUITIN_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P54725-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAVTITLKTL QQQTFKIRME PDETVKVLKE KIEAEKGRDA FPVAGQKLIY
60 70 80 90 100
AGKILSDDVP IRDYRIDEKN FVVVMVTKTK AGQGTSAPPE ASPTAAPESS
110 120 130 140 150
TSFPPAPTSG MSHPPPAARE DKSPSEESAP TTSPESVSGS VPSSGSSGRE
160 170 180 190 200
EDAASTLVTG SEYETMLTEI MSMGYERERV VAALRASYNN PHRAVEYLLT
210 220 230 240 250
GIPGSPEPEH GSVQESQVSE QPATEAAGEN PLEFLRDQPQ FQNMRQVIQQ
260 270 280 290 300
NPALLPALLQ QLGQENPQLL QQISRHQEQF IQMLNEPPGE LADISDVEGE
310 320 330 340 350
VGAIGEEAPQ MNYIQVTPQE KEAIERLKAL GFPESLVIQA YFACEKNENL
360
AANFLLSQNF DDE
Length:363
Mass (Da):39,609
Last modified:October 1, 1996 - v1
Checksum:iC4E47E9313BB47B5
GO
Isoform 2 (identifier: P54725-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     272-326: Missing.

Note: No experimental confirmation available.
Show »
Length:308
Mass (Da):33,433
Checksum:iE09AD30E925EEBAD
GO
Isoform 3 (identifier: P54725-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-226: Missing.

Note: No experimental confirmation available.
Show »
Length:362
Mass (Da):39,538
Checksum:iE6A8DC2ADC3813F6
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7EQ16K7EQ16_HUMAN
UV excision repair protein RAD23 ho...
RAD23A
150Annotation score:
K7ELW1K7ELW1_HUMAN
UV excision repair protein RAD23 ho...
RAD23A
142Annotation score:
K7ENJ0K7ENJ0_HUMAN
UV excision repair protein RAD23 ho...
RAD23A
139Annotation score:
K7ELU6K7ELU6_HUMAN
UV excision repair protein RAD23 ho...
RAD23A
62Annotation score:

Sequence cautioni

The sequence BAD92950 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BX448989 differs from that shown. Reason: Frameshift at positions 159, 333, 339 and 347.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_016251131T → A2 PublicationsCorresponds to variant dbSNP:rs11558955Ensembl.1
Natural variantiVAR_020377179R → Q. Corresponds to variant dbSNP:rs4987203Ensembl.1
Natural variantiVAR_016252200T → M1 PublicationCorresponds to variant dbSNP:rs4987202Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054694226Missing in isoform 3. 2 Publications1
Alternative sequenceiVSP_047565272 – 326Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21235 mRNA Translation: BAA04767.1
AF549209 Genomic DNA Translation: AAN39383.1
BX448989 mRNA No translation available.
AB209713 mRNA Translation: BAD92950.1 Different initiation.
AC092069 Genomic DNA No translation available.
AD000092 Genomic DNA Translation: AAB51177.1
BC014026 mRNA Translation: AAH14026.1
BC088364 mRNA Translation: AAH88364.1
CCDSiCCDS12289.1 [P54725-1]
CCDS59357.1 [P54725-2]
CCDS59358.1 [P54725-3]
PIRiS44443
RefSeqiNP_001257291.1, NM_001270362.1 [P54725-3]
NP_001257292.1, NM_001270363.1 [P54725-2]
NP_005044.1, NM_005053.3 [P54725-1]
UniGeneiHs.643267

Genome annotation databases

EnsembliENST00000316856; ENSP00000321365; ENSG00000179262 [P54725-3]
ENST00000586534; ENSP00000467024; ENSG00000179262 [P54725-1]
ENST00000592268; ENSP00000468674; ENSG00000179262 [P54725-2]
GeneIDi5886
KEGGihsa:5886
UCSCiuc002mvw.3 human [P54725-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D21235 mRNA Translation: BAA04767.1
AF549209 Genomic DNA Translation: AAN39383.1
BX448989 mRNA No translation available.
AB209713 mRNA Translation: BAD92950.1 Different initiation.
AC092069 Genomic DNA No translation available.
AD000092 Genomic DNA Translation: AAB51177.1
BC014026 mRNA Translation: AAH14026.1
BC088364 mRNA Translation: AAH88364.1
CCDSiCCDS12289.1 [P54725-1]
CCDS59357.1 [P54725-2]
CCDS59358.1 [P54725-3]
PIRiS44443
RefSeqiNP_001257291.1, NM_001270362.1 [P54725-3]
NP_001257292.1, NM_001270363.1 [P54725-2]
NP_005044.1, NM_005053.3 [P54725-1]
UniGeneiHs.643267

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DV0NMR-A319-363[»]
1F4INMR-A319-363[»]
1IFYNMR-A156-204[»]
1OQYNMR-A1-363[»]
1P98NMR-A1-78[»]
1P9DNMR-U1-78[»]
1QZENMR-A2-363[»]
1TP4NMR-A223-317[»]
1ZO6model-A315-363[»]
2WYQX-ray1.65A1-82[»]
5XBONMR-B156-204[»]
DisProtiDP00156
ProteinModelPortaliP54725
SMRiP54725
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111823, 246 interactors
DIPiDIP-34442N
IntActiP54725, 73 interactors
MINTiP54725
STRINGi9606.ENSP00000467024

PTM databases

iPTMnetiP54725
PhosphoSitePlusiP54725

Polymorphism and mutation databases

BioMutaiRAD23A
DMDMi1709983

Proteomic databases

EPDiP54725
MaxQBiP54725
PaxDbiP54725
PeptideAtlasiP54725
PRIDEiP54725
ProteomicsDBi56700

Protocols and materials databases

DNASUi5886
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316856; ENSP00000321365; ENSG00000179262 [P54725-3]
ENST00000586534; ENSP00000467024; ENSG00000179262 [P54725-1]
ENST00000592268; ENSP00000468674; ENSG00000179262 [P54725-2]
GeneIDi5886
KEGGihsa:5886
UCSCiuc002mvw.3 human [P54725-1]

Organism-specific databases

CTDi5886
DisGeNETi5886
EuPathDBiHostDB:ENSG00000179262.9
GeneCardsiRAD23A
HGNCiHGNC:9812 RAD23A
HPAiHPA026418
HPA053901
HPA057003
HPA065599
MIMi600061 gene
neXtProtiNX_P54725
OpenTargetsiENSG00000179262
PharmGKBiPA34172
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0011 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00390000012078
HOGENOMiHOG000172162
HOVERGENiHBG055042
InParanoidiP54725
KOiK10839
OMAiKGRDAFP
OrthoDBiEOG091G0DVL
PhylomeDBiP54725

Enzyme and pathway databases

ReactomeiR-HSA-5689877 Josephin domain DUBs
R-HSA-5696394 DNA Damage Recognition in GG-NER
R-HSA-5696395 Formation of Incision Complex in GG-NER

Miscellaneous databases

ChiTaRSiRAD23A human
EvolutionaryTraceiP54725
GeneWikiiRAD23A
GenomeRNAii5886
PMAP-CutDBiP54725
PROiPR:P54725
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000179262 Expressed in 234 organ(s), highest expression level in muscle of leg
CleanExiHS_RAD23A
ExpressionAtlasiP54725 baseline and differential
GenevisibleiP54725 HS

Family and domain databases

Gene3Di1.10.10.540, 1 hit
InterProiView protein in InterPro
IPR004806 Rad23
IPR006636 STI1_HS-bd
IPR015940 UBA
IPR009060 UBA-like_sf
IPR029071 Ubiquitin-like_domsf
IPR000626 Ubiquitin_dom
IPR015360 XPC-bd
IPR036353 XPC-bd_sf
PfamiView protein in Pfam
PF00627 UBA, 2 hits
PF00240 ubiquitin, 1 hit
PF09280 XPC-binding, 1 hit
PRINTSiPR01839 RAD23PROTEIN
SMARTiView protein in SMART
SM00727 STI1, 1 hit
SM00165 UBA, 2 hits
SM00213 UBQ, 1 hit
SUPFAMiSSF101238 SSF101238, 1 hit
SSF46934 SSF46934, 2 hits
SSF54236 SSF54236, 1 hit
TIGRFAMsiTIGR00601 rad23, 1 hit
PROSITEiView protein in PROSITE
PS50030 UBA, 2 hits
PS50053 UBIQUITIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRD23A_HUMAN
AccessioniPrimary (citable) accession number: P54725
Secondary accession number(s): K7ESE3, Q59EU8, Q5M7Z1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 12, 2018
This is version 191 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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