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Protein

Potassium-transporting ATPase alpha chain 2

Gene

ATP12A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for potassium absorption in various tissues.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3914-aspartylphosphate intermediateBy similarity1
Metal bindingi732MagnesiumBy similarity1
Metal bindingi736MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Potassium transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi3.6.3.10 2681
ReactomeiR-HSA-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.1.13 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 2 (EC:3.6.3.10)
Alternative name(s):
Non-gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:ATP12A
Synonyms:ATP1AL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000075673.11
HGNCiHGNC:13816 ATP12A
MIMi182360 gene
neXtProtiNX_P54707

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 102CytoplasmicSequence analysisAdd BLAST102
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 146LumenalSequence analysisAdd BLAST23
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Topological domaini168 – 303CytoplasmicSequence analysisAdd BLAST136
Transmembranei304 – 323HelicalSequence analysisAdd BLAST20
Topological domaini324 – 335LumenalSequence analysisAdd BLAST12
Transmembranei336 – 353HelicalSequence analysisAdd BLAST18
Topological domaini354 – 787CytoplasmicSequence analysisAdd BLAST434
Transmembranei788 – 807HelicalSequence analysisAdd BLAST20
Topological domaini808 – 817LumenalSequence analysis10
Transmembranei818 – 838HelicalSequence analysisAdd BLAST21
Topological domaini839 – 858CytoplasmicSequence analysisAdd BLAST20
Transmembranei859 – 881HelicalSequence analysisAdd BLAST23
Topological domaini882 – 933LumenalSequence analysisAdd BLAST52
Transmembranei934 – 953HelicalSequence analysisAdd BLAST20
Topological domaini954 – 967CytoplasmicSequence analysisAdd BLAST14
Transmembranei968 – 986HelicalSequence analysisAdd BLAST19
Topological domaini987 – 1001LumenalSequence analysisAdd BLAST15
Transmembranei1002 – 1022HelicalSequence analysisAdd BLAST21
Topological domaini1023 – 1039CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi479
OpenTargetsiENSG00000075673
PharmGKBiPA25104

Chemistry databases

ChEMBLiCHEMBL2933

Polymorphism and mutation databases

BioMutaiATP12A
DMDMi212287925

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462601 – 1039Potassium-transporting ATPase alpha chain 2Add BLAST1039

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei958Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP54707
MaxQBiP54707
PaxDbiP54707
PeptideAtlasiP54707
PRIDEiP54707
ProteomicsDBi56695
56696 [P54707-2]

PTM databases

iPTMnetiP54707
PhosphoSitePlusiP54707
SwissPalmiP54707

Expressioni

Tissue specificityi

Found in skin and kidney. Detected in prostate basal cells (at protein level). Expression is increased in benign prostate hyperplasia and tumor tissues (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000075673 Expressed in 58 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_ATP12A
GenevisibleiP54707 HS

Organism-specific databases

HPAiHPA039526

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

BioGridi106969, 85 interactors
IntActiP54707, 4 interactors
STRINGi9606.ENSP00000218548

Chemistry databases

BindingDBiP54707

Structurei

3D structure databases

ProteinModelPortaliP54707
SMRiP54707
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
HOGENOMiHOG000265622
HOVERGENiHBG004298
InParanoidiP54707
KOiK01544
OMAiHSNQVFD
OrthoDBiEOG091G01BB
PhylomeDBiP54707
TreeFamiTF312838

Family and domain databases

CDDicd02608 P-type_ATPase_Na-K_like, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR030318 Atp12a
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005775 P-type_ATPase_IIC
IPR001757 P_typ_ATPase
PANTHERiPTHR43294:SF1 PTHR43294:SF1, 1 hit
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01106 ATPase-IIC_X-K, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P54707-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHQKTPEIYS VELSGTKDIV KTDKGDGKEK YRGLKNNCLE LKKKNHKEEF
60 70 80 90 100
QKELHLDDHK LSNRELEEKY GTDIIMGLSS TRAAELLARD GPNSLTPPKQ
110 120 130 140 150
TPEIVKFLKQ MVGGFSILLW VGAFLCWIAY GIQYSSDKSA SLNNVYLGCV
160 170 180 190 200
LGLVVILTGI FAYYQEAKST NIMSSFNKMI PQQALVIRDS EKKTIPSEQL
210 220 230 240 250
VVGDIVEVKG GDQIPADIRV LSSQGCRVDN SSLTGESEPQ PRSSEFTHEN
260 270 280 290 300
PLETKNICFY STTCLEGTVT GMVINTGDRT IIGHIASLAS GVGNEKTPIA
310 320 330 340 350
IEIEHFVHIV AGVAVSIGIL FFIIAVSLKY QVLDSIIFLI GIIVANVPEG
360 370 380 390 400
LLATVTVTLS LTAKRMAKKN CLVKNLEAVE TLGSTSIICS DKTGTLTQNR
410 420 430 440 450
MTVAHLWFDN QIFVADTSED HSNQVFDQSS RTWASLSKII TLCNRAEFKP
460 470 480 490 500
GQENVPIMKK AVIGDASETA LLKFSEVILG DVMEIRKRNR KVAEIPFNST
510 520 530 540 550
NKFQLSIHEM DDPHGKRFLM VMKGAPERIL EKCSTIMING EEHPLDKSTA
560 570 580 590 600
KTFHTAYMEL GGLGERVLGF CHLYLPADEF PETYSFDIDA MNFPTSNLCF
610 620 630 640 650
VGLLSMIDPP RSTVPDAVTK CRSAGIKVIM VTGDHPITAK AIAKSVGIIS
660 670 680 690 700
ANSETVEDIA HRLNIAVEQV NKRDAKAAVV TGMELKDMSS EQLDEILANY
710 720 730 740 750
QEIVFARTSP QQKLIIVEGC QRQDAVVAVT GDGVNDSPAL KKADIGIAMG
760 770 780 790 800
IAGSDAAKNA ADMVLLDDNF ASIVTGVEEG RLIFDNLKKT IAYSLTKNIA
810 820 830 840 850
ELCPFLIYII VGLPLPIGTI TILFIDLGTD IIPSIALAYE KAESDIMNRK
860 870 880 890 900
PRHKNKDRLV NQPLAVYSYL HIGLMQALGA FLVYFTVYAQ EGFLPRTLIN
910 920 930 940 950
LRVEWEKDYV NDLKDSYGQE WTRYQREYLE WTGYTAFFVG ILVQQIADLI
960 970 980 990 1000
IRKTRRNSIF QQGLFRNKVI WVGITSQIII GLILSYGLGS VTALSFTMLR
1010 1020 1030
AQYWFVAVPH AILIWVYDEV RKLFIRLYPG SWWDKNMYY
Length:1,039
Mass (Da):115,511
Last modified:July 22, 2008 - v3
Checksum:iC906897E11FA406C
GO
Isoform 2 (identifier: P54707-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     266-266: E → EASTSPV

Note: No experimental confirmation available.
Show »
Length:1,045
Mass (Da):116,053
Checksum:i15088FC158FB580A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3Q → QLFQ in AAC37589 (PubMed:8838794).Curated1
Sequence conflicti145V → VRLWGVQV in CAA49477 (PubMed:1847115).Curated1
Sequence conflicti225 – 227Missing in CAA49477 (PubMed:1847115).Curated3
Sequence conflicti266E → V in CAA49477 (PubMed:1847115).Curated1
Sequence conflicti382L → P in AAA35576 (PubMed:3036582).Curated1
Sequence conflicti724 – 727DAVV → VGGQ in CAA49478 (PubMed:1847115).Curated4
Sequence conflicti772 – 773SI → LH in CAA49478 (PubMed:1847115).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_020186863P → L. Corresponds to variant dbSNP:rs2289909Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034640266E → EASTSPV in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02076 mRNA Translation: AAB37755.1
L42563
, L42558, L42559, L42565, L42566, L42567, L42560, L42561, L42568, L42562, L42569 Genomic DNA Translation: AAC37589.2
AK292968 mRNA Translation: BAF85657.1
AL157364 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08346.1
CH471075 Genomic DNA Translation: EAX08345.1
BC031609 mRNA Translation: AAH31609.1
X69823 Genomic DNA Translation: CAA49477.1
X69824 Genomic DNA Translation: CAA49478.1
M16797 mRNA Translation: AAA51802.1
M27574 Genomic DNA Translation: AAA35576.1
CCDSiCCDS31948.1 [P54707-1]
CCDS53858.1 [P54707-2]
PIRiA26641
D27795
E27397
I38401
S13028
S31504
RefSeqiNP_001172014.1, NM_001185085.1 [P54707-2]
NP_001667.4, NM_001676.5 [P54707-1]
UniGeneiHs.147111

Genome annotation databases

EnsembliENST00000218548; ENSP00000218548; ENSG00000075673 [P54707-2]
ENST00000381946; ENSP00000371372; ENSG00000075673 [P54707-1]
GeneIDi479
KEGGihsa:479
UCSCiuc001upp.4 human [P54707-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02076 mRNA Translation: AAB37755.1
L42563
, L42558, L42559, L42565, L42566, L42567, L42560, L42561, L42568, L42562, L42569 Genomic DNA Translation: AAC37589.2
AK292968 mRNA Translation: BAF85657.1
AL157364 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08346.1
CH471075 Genomic DNA Translation: EAX08345.1
BC031609 mRNA Translation: AAH31609.1
X69823 Genomic DNA Translation: CAA49477.1
X69824 Genomic DNA Translation: CAA49478.1
M16797 mRNA Translation: AAA51802.1
M27574 Genomic DNA Translation: AAA35576.1
CCDSiCCDS31948.1 [P54707-1]
CCDS53858.1 [P54707-2]
PIRiA26641
D27795
E27397
I38401
S13028
S31504
RefSeqiNP_001172014.1, NM_001185085.1 [P54707-2]
NP_001667.4, NM_001676.5 [P54707-1]
UniGeneiHs.147111

3D structure databases

ProteinModelPortaliP54707
SMRiP54707
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106969, 85 interactors
IntActiP54707, 4 interactors
STRINGi9606.ENSP00000218548

Chemistry databases

BindingDBiP54707
ChEMBLiCHEMBL2933

Protein family/group databases

TCDBi3.A.3.1.13 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetiP54707
PhosphoSitePlusiP54707
SwissPalmiP54707

Polymorphism and mutation databases

BioMutaiATP12A
DMDMi212287925

Proteomic databases

EPDiP54707
MaxQBiP54707
PaxDbiP54707
PeptideAtlasiP54707
PRIDEiP54707
ProteomicsDBi56695
56696 [P54707-2]

Protocols and materials databases

DNASUi479
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000218548; ENSP00000218548; ENSG00000075673 [P54707-2]
ENST00000381946; ENSP00000371372; ENSG00000075673 [P54707-1]
GeneIDi479
KEGGihsa:479
UCSCiuc001upp.4 human [P54707-1]

Organism-specific databases

CTDi479
DisGeNETi479
EuPathDBiHostDB:ENSG00000075673.11
GeneCardsiATP12A
HGNCiHGNC:13816 ATP12A
HPAiHPA039526
MIMi182360 gene
neXtProtiNX_P54707
OpenTargetsiENSG00000075673
PharmGKBiPA25104
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0203 Eukaryota
COG0474 LUCA
GeneTreeiENSGT00890000139334
HOGENOMiHOG000265622
HOVERGENiHBG004298
InParanoidiP54707
KOiK01544
OMAiHSNQVFD
OrthoDBiEOG091G01BB
PhylomeDBiP54707
TreeFamiTF312838

Enzyme and pathway databases

BRENDAi3.6.3.10 2681
ReactomeiR-HSA-936837 Ion transport by P-type ATPases

Miscellaneous databases

ChiTaRSiATP12A human
GeneWikiiATP12A
GenomeRNAii479
PROiPR:P54707
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000075673 Expressed in 58 organ(s), highest expression level in epithelium of bronchus
CleanExiHS_ATP12A
GenevisibleiP54707 HS

Family and domain databases

CDDicd02608 P-type_ATPase_Na-K_like, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR030318 Atp12a
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR005775 P-type_ATPase_IIC
IPR001757 P_typ_ATPase
PANTHERiPTHR43294:SF1 PTHR43294:SF1, 1 hit
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01106 ATPase-IIC_X-K, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAT12A_HUMAN
AccessioniPrimary (citable) accession number: P54707
Secondary accession number(s): Q13816
, Q13817, Q16734, Q5W035, Q8N5U2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 22, 2008
Last modified: November 7, 2018
This is version 186 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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