UniProtKB - P54690 (BCAT1_RAT)
Protein
Branched-chain-amino-acid aminotransferase, cytosolic
Gene
Bcat1
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Catalytic activityi
Cofactori
GO - Molecular functioni
- branched-chain-amino-acid transaminase activity Source: RGD
- identical protein binding Source: RGD
- L-isoleucine transaminase activity Source: UniProtKB-EC
- L-leucine transaminase activity Source: UniProtKB-EC
- L-valine transaminase activity Source: UniProtKB-EC
GO - Biological processi
- branched-chain amino acid catabolic process Source: RGD
- leucine biosynthetic process Source: GO_Central
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
Reactomei | R-RNO-70895, Branched-chain amino acid catabolism |
SABIO-RKi | P54690 |
Names & Taxonomyi
Protein namesi | Recommended name: Branched-chain-amino-acid aminotransferase, cytosolic (EC:2.6.1.42)Short name: BCAT(c) |
Gene namesi | Name:Bcat1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2195, Bcat1 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrion Source: GO_Central
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103294 | 1 – 411 | Branched-chain-amino-acid aminotransferase, cytosolicAdd BLAST | 411 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 247 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.
Proteomic databases
jPOSTi | P54690 |
PaxDbi | P54690 |
PRIDEi | P54690 |
PTM databases
iPTMneti | P54690 |
PhosphoSitePlusi | P54690 |
SwissPalmi | P54690 |
Expressioni
Tissue specificityi
Brain, low expression in ovary and placenta, but not found in liver, kidney, and skeletal muscle.
Gene expression databases
Bgeei | ENSRNOG00000015514, Expressed in brain and 22 other tissues |
ExpressionAtlasi | P54690, baseline and differential |
Genevisiblei | P54690, RN |
Interactioni
Subunit structurei
Homodimer.
GO - Molecular functioni
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 248224, 1 interactor |
STRINGi | 10116.ENSRNOP00000021193 |
Chemistry databases
BindingDBi | P54690 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 31 | Poly-Glu | 6 |
Sequence similaritiesi
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
InParanoidi | P54690 |
OMAi | LTEVFAC |
OrthoDBi | 853728at2759 |
PhylomeDBi | P54690 |
TreeFami | TF300882 |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P54690-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAYLSRATAT LARQDCSNGC SASYAEEEEL EASTESYDEE GGSEASTQTF
60 70 80 90 100
RAKDLIITKA DVLKKKPDPS SLVFGASFTD HMLMVEWTSK YGWDKPHIKP
110 120 130 140 150
FENLSIHPAA SVLHYAVELF EGLKAFRGVD NKIRLFRPDL NMKRMCRSAV
160 170 180 190 200
RTTLPEFDKE ELLQCVLQLI QLDREWVPYS TSASLYIRPT FIGIEPSLGV
210 220 230 240 250
KKPSKALLFV ILSPVGSYFS NGTFSPVSLW ANPKFVRSWK GGTGDFKMGC
260 270 280 290 300
NYGSSLLAQC EAAENGCHQV LWLYGKENRI TEVGTMNLFL YWINKDGEEE
310 320 330 340 350
LATPPLDGVI LPGVTRQSIL ELGEEWGEFK VCERHITMDD LSTALEENRV
360 370 380 390 400
KEMFGSGTAC VVCPVASILY KGQMLHIPTM ENGHKLSSRI MAKLTDIQYG
410
RIKSEWTLEL P
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ99JD5 | Q99JD5_RAT | Branched-chain-amino-acid aminotran... | Bcat1 Bcatc, rCG_29894 | 399 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35774 mRNA Translation: AAC52385.1 BC087710 mRNA Translation: AAH87710.1 |
RefSeqi | NP_058949.1, NM_017253.2 |
Genome annotation databases
Ensembli | ENSRNOT00000021193; ENSRNOP00000021193; ENSRNOG00000015514 |
GeneIDi | 29592 |
KEGGi | rno:29592 |
UCSCi | RGD:2195, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U35774 mRNA Translation: AAC52385.1 BC087710 mRNA Translation: AAH87710.1 |
RefSeqi | NP_058949.1, NM_017253.2 |
3D structure databases
SMRi | P54690 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 248224, 1 interactor |
STRINGi | 10116.ENSRNOP00000021193 |
Chemistry databases
BindingDBi | P54690 |
ChEMBLi | CHEMBL4678 |
PTM databases
iPTMneti | P54690 |
PhosphoSitePlusi | P54690 |
SwissPalmi | P54690 |
Proteomic databases
jPOSTi | P54690 |
PaxDbi | P54690 |
PRIDEi | P54690 |
Genome annotation databases
Ensembli | ENSRNOT00000021193; ENSRNOP00000021193; ENSRNOG00000015514 |
GeneIDi | 29592 |
KEGGi | rno:29592 |
UCSCi | RGD:2195, rat |
Organism-specific databases
CTDi | 586 |
RGDi | 2195, Bcat1 |
Phylogenomic databases
eggNOGi | KOG0975, Eukaryota |
GeneTreei | ENSGT00390000009532 |
InParanoidi | P54690 |
OMAi | LTEVFAC |
OrthoDBi | 853728at2759 |
PhylomeDBi | P54690 |
TreeFami | TF300882 |
Enzyme and pathway databases
Reactomei | R-RNO-70895, Branched-chain amino acid catabolism |
SABIO-RKi | P54690 |
Miscellaneous databases
PROi | PR:P54690 |
Gene expression databases
Bgeei | ENSRNOG00000015514, Expressed in brain and 22 other tissues |
ExpressionAtlasi | P54690, baseline and differential |
Genevisiblei | P54690, RN |
Family and domain databases
CDDi | cd01557, BCAT_beta_family, 1 hit |
Gene3Di | 3.20.10.10, 1 hit 3.30.470.10, 1 hit |
InterProi | View protein in InterPro IPR001544, Aminotrans_IV IPR018300, Aminotrans_IV_CS IPR036038, Aminotransferase-like IPR005786, B_amino_transII IPR043132, BCAT-like_C IPR043131, BCAT-like_N IPR033939, BCAT_family |
Pfami | View protein in Pfam PF01063, Aminotran_4, 1 hit |
PIRSFi | PIRSF006468, BCAT1, 1 hit |
SUPFAMi | SSF56752, SSF56752, 1 hit |
TIGRFAMsi | TIGR01123, ilvE_II, 1 hit |
PROSITEi | View protein in PROSITE PS00770, AA_TRANSFER_CLASS_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAT1_RAT | |
Accessioni | P54690Primary (citable) accession number: P54690 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families