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Protein

RAC family serine/threonine-protein kinase homolog

Gene

pkbA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Predominantly involved during the aggregation to control cell polarity and chemotaxis. Phosphorylates talB, gefN, gefS, PI4P 5-kinase and gacQ.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149ATPPROSITE-ProRule annotation1
Active sitei243Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 134ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: dictyBase
  • cAMP-dependent protein kinase activity Source: dictyBase
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: dictyBase
  • protein kinase regulator activity Source: dictyBase
  • protein serine/threonine kinase activity Source: dictyBase

GO - Biological processi

  • activation of protein kinase activity Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • culmination involved in sorocarp development Source: dictyBase
  • establishment of cell polarity Source: dictyBase
  • establishment or maintenance of cell polarity Source: dictyBase
  • macropinocytosis Source: dictyBase
  • mitotic cytokinesis Source: dictyBase
  • myosin II filament assembly Source: dictyBase
  • negative regulation of phagocytosis Source: dictyBase
  • negative regulation of Ras protein signal transduction Source: dictyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • phagosome maturation Source: dictyBase
  • phosphatidylinositol-mediated signaling Source: dictyBase
  • polyphosphate-mediated signaling Source: dictyBase
  • positive regulation of macropinocytosis Source: dictyBase
  • protein phosphorylation Source: dictyBase
  • regulation of chemotaxis Source: dictyBase
  • regulation of protein kinase activity Source: dictyBase
  • response to differentiation-inducing factor 1 Source: dictyBase
  • response to hydrogen peroxide Source: dictyBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processChemotaxis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RAC family serine/threonine-protein kinase homolog (EC:2.7.11.1)
Gene namesi
Name:pkbA
Synonyms:akt, dagA
ORF Names:DDB_G0268620
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0268620 pkbA

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: dictyBase

Pathology & Biotechi

Disruption phenotypei

Cells exhibit aggregation-stage defects, with the aggregates that form producing normal fruiting bodies.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861921 – 444RAC family serine/threonine-protein kinase homologAdd BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP54644
PRIDEiP54644

PTM databases

iPTMnetiP54644

Expressioni

Developmental stagei

Preferentially expressed during growth and aggregation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi1241576, 1 interactor
STRINGi44689.DDB0191195

Structurei

3D structure databases

ProteinModelPortaliP54644
SMRiP54644
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 100PHPROSITE-ProRule annotationAdd BLAST96
Domaini120 – 374Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini375 – 444AGC-kinase C-terminalAdd BLAST70

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0598 Eukaryota
ENOG410XNPH LUCA
InParanoidiP54644
KOiK04456
OMAiEVKEPCN
PhylomeDBiP54644

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P54644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTAPIKHEG FLTKEGGGFK SWKKRWFILK GGDLSYYKTK GELVPLGVIH
60 70 80 90 100
LNTSGHIKNS DRKKRVNGFE VQTPSRTYFL CSETEEERAK WIEILINERE
110 120 130 140 150
LLLNGGKQPK KSEKVGVADF ELLNLVGKGS FGKVIQVRKK DTGEVYAMKV
160 170 180 190 200
LSKKHIVEHN EVEHTLSERN ILQKINHPFL VNLNYSFQTE DKLYFILDYV
210 220 230 240 250
NGGELFYHLQ KDKKFTEDRV RYYGAEIVLA LEHLHLSGVI YRDLKPENLL
260 270 280 290 300
LTNEGHICMT DFGLCKEGLL TPTDKTGTFC GTPEYLAPEV LQGNGYGKQV
310 320 330 340 350
DWWSFGSLLY EMLTGLPPFY NQDVQEMYRK IMMEKLSFPH FISPDARSLL
360 370 380 390 400
EQLLERDPEK RLADPNLIKR HPFFRSIDWE QLFQKNIPPP FIPNVKGSAD
410 420 430 440
TSQIDPVFTD EAPSLTMAGE CALNPQQQKD FEGFTYVAES EHLR
Length:444
Mass (Da):51,062
Last modified:October 1, 1996 - v1
Checksum:i12367A1A411C5680
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15210 mRNA Translation: AAA76692.1
AAFI02000004 Genomic DNA Translation: EAL72899.1
RefSeqiXP_646888.1, XM_641796.1

Genome annotation databases

EnsemblProtistsiEAL72899; EAL72899; DDB_G0268620
GeneIDi8616573
KEGGiddi:DDB_G0268620

Similar proteinsi

Entry informationi

Entry nameiKRAC_DICDI
AccessioniPrimary (citable) accession number: P54644
Secondary accession number(s): Q55EY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

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