UniProtKB - P54622 (SSBP_DROME)
Protein
Single-stranded DNA-binding protein, mitochondrial
Gene
mtSSB
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed:7673145, PubMed:8206370, PubMed:21953457). Required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the gamma complex polymerase tam at the replication fork (PubMed:21953457, PubMed:26446790, PubMed:14754882, PubMed:28318978, PubMed:7673145, PubMed:11294889). Promotes tam activity largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate tam activity (PubMed:26446790, PubMed:21953457, PubMed:7673145).7 Publications
GO - Molecular functioni
- single-stranded DNA binding Source: UniProtKB
GO - Biological processi
- DNA synthesis involved in DNA replication Source: FlyBase
- DNA unwinding involved in DNA replication Source: FlyBase
- mitochondrial DNA replication Source: UniProtKB
- mitochondrial genome maintenance Source: UniProtKB
- positive regulation of DNA-directed DNA polymerase activity Source: FlyBase
- positive regulation of exodeoxyribonuclease activity Source: FlyBase
- positive regulation of helicase activity Source: GO_Central
- positive regulation of mitochondrial DNA replication Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Single-stranded DNA-binding protein, mitochondrialShort name: Dm mtSSB Short name: Mt-SSB Short name: MtSSB Alternative name(s): Protein low power |
Gene namesi | Name:mtSSB Synonyms:lopo ORF Names:CG4337 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0010438, mtSSB |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrial chromosome Source: FlyBase
- mitochondrial nucleoid Source: GO_Central
- mitochondrion Source: UniProtKB
Other locations
- nucleoid Source: GO_Central
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown in the testes results in a reduced number of paternal mitochondrial nucleoids at the nebenkern stage of spermatogenesis (PubMed:28318978). However, the clearance of nucleoids at the elongation stage of spermatogenesis is unaffected (PubMed:28318978).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 57 – 58 | QE → AA in mtSSBloop12; significant reduction in mitochondrial DNA copy number. 1 Publication | 2 | |
Mutagenesisi | 74 – 76 | Missing in mtSSBloop23; significant reduction in mitochondrial DNA copy number. No effect on ability to stimulate the activity of the polymerase tam. 2 Publications | 3 | |
Mutagenesisi | 85 | W → A: Reduced DNA synthesis by the polymerase tam. 1 Publication | 1 | |
Mutagenesisi | 85 | W → T: Reduced DNA synthesis by the polymerase tam. Severe decrease in the stimulation of DNA synthesis by the polymerase tam and decreased DNA binding; when associated with A-91. 1 Publication | 1 | |
Mutagenesisi | 85 | W → Y: No effect on DNA synthesis by the polymerase tam. 1 Publication | 1 | |
Mutagenesisi | 91 | F → A: No decrease in DNA synthesis by the polymerase tam. Severe decrease DNA synthesis by the polymerase tam and decreased DNA binding; when associated with T-85. 1 Publication | 1 | |
Mutagenesisi | 100 – 101 | LE → AA in mtSSBalpha1; significant reduction in mitochondrial DNA (mtDNA) copy number and reduced recovery of mtDNA depletion induced by ethidium bromide. 1 Publication | 2 | |
Mutagenesisi | 117 – 119 | YGE → AAA in mtSSB45-1; significant reduction in mitochondrial DNA (mtDNA) copy number and reduced recovery of mtDNA depletion induced by ethidium bromide. 1 Publication | 3 | |
Mutagenesisi | 123 – 125 | QQG → AAA in mtSSB45-2; reduced mitochondrial DNA (mtDNA) copy number and reduced recovery of mtDNA depletion induced by ethidium bromide. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 22 | Mitochondrion1 PublicationAdd BLAST | 22 | |
ChainiPRO_0000033268 | 23 – 146 | Single-stranded DNA-binding protein, mitochondrialAdd BLAST | 124 |
Proteomic databases
PaxDbi | P54622 |
PRIDEi | P54622 |
Expressioni
Tissue specificityi
Uniformly distributed in the early embryo. High levels detected in the anterior and posterior midgut primordia of stage 12 embryos. In larvae, high levels were detected in proliferating tissues including the CNS and digestive tract. In adults, highly expressed in the CNS, digestive tract and ovary.3 Publications
Developmental stagei
Expressed both maternally and zygotically. Levels are high during embryogenesis and in the larvae but decrease in the pupae before increasing again in the adult.3 Publications
Gene expression databases
Bgeei | FBgn0010438, Expressed in adult organism and 23 other tissues |
Genevisiblei | P54622, DM |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 67018, 18 interactors |
DIPi | DIP-22042N |
IntActi | P54622, 6 interactors |
MINTi | P54622 |
STRINGi | 7227.FBpp0099503 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 142 | SSBPROSITE-ProRule annotationAdd BLAST | 105 |
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1653, Eukaryota |
GeneTreei | ENSGT00390000002796 |
HOGENOMi | CLU_078758_2_1_1 |
InParanoidi | P54622 |
OMAi | SLATHTN |
OrthoDBi | 1304218at2759 |
PhylomeDBi | P54622 |
Family and domain databases
CDDi | cd04496, SSB_OBF, 1 hit |
HAMAPi | MF_00984, SSB, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR000424, Primosome_PriB/ssb IPR011344, ssDNA-bd |
PANTHERi | PTHR10302, PTHR10302, 1 hit |
Pfami | View protein in Pfam PF00436, SSB, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00621, ssb, 1 hit |
PROSITEi | View protein in PROSITE PS50935, SSB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P54622-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQHTRRMLNP LLTGLRNLPA RGATTTTAAA PAKVEKTVNT VTILGRVGAD
60 70 80 90 100
PQLRGSQEHP VVTFSVATHT NYKYENGDWA QRTDWHRVVV FKPNLRDTVL
110 120 130 140
EYLKKGQRTM VQGKITYGEI TDQQGNQKTS TSIIADDVLF FRDANN
Sequence cautioni
The sequence AAA20507 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAF16936 differs from that shown. Reason: Erroneous gene model prediction.Curated
Mass spectrometryi
Molecular mass is 13845±14 Da. Determined by MALDI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF181084 Genomic DNA Translation: AAF16936.1 Sequence problems. AE014297 Genomic DNA Translation: AAF55287.2 BT016028 mRNA Translation: AAV36913.2 U00669 mRNA Translation: AAA20507.1 Different initiation. |
RefSeqi | NP_536744.2, NM_080496.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0083277; FBpp0082729; FBgn0010438 |
GeneIDi | 41968 |
KEGGi | dme:Dmel_CG4337 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF181084 Genomic DNA Translation: AAF16936.1 Sequence problems. AE014297 Genomic DNA Translation: AAF55287.2 BT016028 mRNA Translation: AAV36913.2 U00669 mRNA Translation: AAA20507.1 Different initiation. |
RefSeqi | NP_536744.2, NM_080496.4 |
3D structure databases
SMRi | P54622 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 67018, 18 interactors |
DIPi | DIP-22042N |
IntActi | P54622, 6 interactors |
MINTi | P54622 |
STRINGi | 7227.FBpp0099503 |
Proteomic databases
PaxDbi | P54622 |
PRIDEi | P54622 |
Genome annotation databases
EnsemblMetazoai | FBtr0083277; FBpp0082729; FBgn0010438 |
GeneIDi | 41968 |
KEGGi | dme:Dmel_CG4337 |
Organism-specific databases
CTDi | 41968 |
FlyBasei | FBgn0010438, mtSSB |
Phylogenomic databases
eggNOGi | KOG1653, Eukaryota |
GeneTreei | ENSGT00390000002796 |
HOGENOMi | CLU_078758_2_1_1 |
InParanoidi | P54622 |
OMAi | SLATHTN |
OrthoDBi | 1304218at2759 |
PhylomeDBi | P54622 |
Miscellaneous databases
BioGRID-ORCSi | 41968, 1 hit in 1 CRISPR screen |
GenomeRNAii | 41968 |
PROi | PR:P54622 |
Gene expression databases
Bgeei | FBgn0010438, Expressed in adult organism and 23 other tissues |
Genevisiblei | P54622, DM |
Family and domain databases
CDDi | cd04496, SSB_OBF, 1 hit |
HAMAPi | MF_00984, SSB, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR000424, Primosome_PriB/ssb IPR011344, ssDNA-bd |
PANTHERi | PTHR10302, PTHR10302, 1 hit |
Pfami | View protein in Pfam PF00436, SSB, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00621, ssb, 1 hit |
PROSITEi | View protein in PROSITE PS50935, SSB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSBP_DROME | |
Accessioni | P54622Primary (citable) accession number: P54622 Secondary accession number(s): Q5U164, Q9V3U4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 25, 2003 | |
Last modified: | December 2, 2020 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase