We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P54577 (SYYC_HUMAN)
Protein
Tyrosine--tRNA ligase, cytoplasmic
Gene
YARS1
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
By similarityCatalytic activityi
- EC:6.1.1.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 39 | TyrosineCombined sources1 Publication | 1 | |
Binding sitei | 166 | TyrosineCombined sources1 Publication | 1 | |
Binding sitei | 170 | TyrosineCombined sources1 Publication | 1 | |
Binding sitei | 173 | TyrosineCombined sources1 Publication | 1 | |
Binding sitei | 188 | TyrosineCombined sources1 Publication | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- interleukin-8 receptor binding Source: ProtInc
- RNA binding Source: UniProtKB
- tRNA binding Source: UniProtKB-KW
- tyrosine-tRNA ligase activity Source: GO_Central
GO - Biological processi
- apoptotic process Source: ProtInc
- tyrosyl-tRNA aminoacylation Source: ProtInc
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.1, 2681 |
PathwayCommonsi | P54577 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
SignaLinki | P54577 |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine--tRNA ligase, cytoplasmic (EC:6.1.1.1)Alternative name(s): Tyrosyl-tRNA synthetase Short name: TyrRS Cleaved into the following chain: |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:12840, YARS1 |
MIMi | 603623, gene |
neXtProti | NX_P54577 |
VEuPathDBi | HostDB:ENSG00000134684 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular space Source: ProtInc
Nucleus
- nuclear body Source: HPA
Other locations
- cytoplasm Source: ProtInc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Involvement in diseasei
Charcot-Marie-Tooth disease, dominant, intermediate type, C (CMTDIC)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. The dominant intermediate type C is characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026681 | 41 | G → R in CMTDIC; partial loss of activity. 1 PublicationCorresponds to variant dbSNP:rs121908833EnsemblClinVar. | 1 | |
Natural variantiVAR_026682 | 153 – 156 | Missing in CMTDIC. 1 Publication | 4 | |
Natural variantiVAR_026684 | 196 | E → K in CMTDIC; partial loss of activity. 1 PublicationCorresponds to variant dbSNP:rs121908834EnsemblClinVar. | 1 |
Keywords - Diseasei
Charcot-Marie-Tooth disease, Disease variant, Neurodegeneration, NeuropathyOrganism-specific databases
DisGeNETi | 8565 |
GeneReviewsi | YARS1 |
MalaCardsi | YARS1 |
MIMi | 608323, phenotype |
OpenTargetsi | ENSG00000134684 |
Orphaneti | 100045, Autosomal dominant intermediate Charcot-Marie-Tooth disease type C |
PharmGKBi | PA37431 |
Miscellaneous databases
Pharosi | P54577, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3179 |
DrugBanki | DB08617, 4-(2,2,2-TRIFLUOROETHYL)-L-PHENYLALANINE DB01766, Beta-(2-Naphthyl)-Alanine DB07205, N6-ISOPENTENYL-ADENOSINE-5'-MONOPHOSPHATE DB08371, p-Benzoyl-L-phenylalanine DB02709, Resveratrol DB03978, Tyrosinal DB00135, Tyrosine |
DrugCentrali | P54577 |
Genetic variation databases
BioMutai | YARS |
DMDMi | 13638438 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000423285 | 1 – 528 | Tyrosine--tRNA ligase, cytoplasmicAdd BLAST | 528 | |
Initiator methioninei | Removed; alternateCombined sources2 Publications | |||
ChainiPRO_0000055673 | 2 – 528 | Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedAdd BLAST | 527 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 2 | N-acetylglycine; in Tyrosine--tRNA ligase, cytoplasmic, N-terminally processedCombined sources1 Publication | 1 | |
Modified residuei | 197 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 205 | PhosphoserineCombined sources | 1 | |
Modified residuei | 206 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 386 | PhosphoserineCombined sources | 1 | |
Modified residuei | 474 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 482 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 490 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | CPTAC-299 CPTAC-300 |
EPDi | P54577 |
jPOSTi | P54577 |
MassIVEi | P54577 |
MaxQBi | P54577 |
PaxDbi | P54577 |
PeptideAtlasi | P54577 |
PRIDEi | P54577 |
ProteomicsDBi | 56686 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00007074 |
PTM databases
CarbonylDBi | P54577 |
GlyGeni | P54577, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P54577 |
MetOSitei | P54577 |
PhosphoSitePlusi | P54577 |
SwissPalmi | P54577 |
Expressioni
Gene expression databases
Bgeei | ENSG00000134684, Expressed in frontal cortex and 238 other tissues |
ExpressionAtlasi | P54577, baseline and differential |
Genevisiblei | P54577, HS |
Organism-specific databases
HPAi | ENSG00000134684, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
P54577
GO - Molecular functioni
- interleukin-8 receptor binding Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 114134, 87 interactors |
DIPi | DIP-50415N |
IntActi | P54577, 43 interactors |
MINTi | P54577 |
STRINGi | 9606.ENSP00000362576 |
Chemistry databases
BindingDBi | P54577 |
Miscellaneous databases
RNActi | P54577, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P54577 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54577 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 364 – 468 | tRNA-bindingPROSITE-ProRule annotationAdd BLAST | 105 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 339 – 363 | DisorderedSequence analysisAdd BLAST | 25 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 44 – 52 | 'HIGH' region | 9 | |
Motifi | 222 – 226 | 'KMSKS' region | 5 |
Sequence similaritiesi
Belongs to the class-I aminoacyl-tRNA synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
GeneTreei | ENSGT00940000156949 |
HOGENOMi | CLU_035267_3_0_1 |
InParanoidi | P54577 |
OMAi | YIGFEIS |
OrthoDBi | 852081at2759 |
PhylomeDBi | P54577 |
TreeFami | TF300898 |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
P54577-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV
60 70 80 90 100
AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV
110 120 130 140 150
IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA
160 170 180 190 200
EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP
210 220 230 240 250
ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC
260 270 280 290 300
EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA
310 320 330 340 350
AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM
360 370 380 390 400
AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR
410 420 430 440 450
TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR
460 470 480 490 500
QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE
510 520
CIAQWKQTNF MTKLGSISCK SLKGGNIS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A6Q8PFX2 | A0A6Q8PFX2_HUMAN | Tyrosine--tRNA ligase | YARS1 | 479 | Annotation score: | ||
A0A0C4DGZ5 | A0A0C4DGZ5_HUMAN | Tyrosine--tRNA ligase | YARS1 | 225 | Annotation score: | ||
A0A6Q8PFX4 | A0A6Q8PFX4_HUMAN | Tyrosine--tRNA ligase | YARS1 | 246 | Annotation score: | ||
A0A6Q8PF15 | A0A6Q8PF15_HUMAN | Tyrosyl-tRNA synthetase | YARS1 | 218 | Annotation score: | ||
A0A6Q8PFC1 | A0A6Q8PFC1_HUMAN | Tyrosine--tRNA ligase, cytoplasmic | YARS1 | 133 | Annotation score: | ||
A0A6Q8PGW2 | A0A6Q8PGW2_HUMAN | Tyrosine--tRNA ligase, cytoplasmic | YARS1 | 42 | Annotation score: |
Sequence cautioni
The sequence AAB39406 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 143 | H → R in BAD97328 (Ref. 4) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026681 | 41 | G → R in CMTDIC; partial loss of activity. 1 PublicationCorresponds to variant dbSNP:rs121908833EnsemblClinVar. | 1 | |
Natural variantiVAR_026682 | 153 – 156 | Missing in CMTDIC. 1 Publication | 4 | |
Natural variantiVAR_026683 | 170 | Q → H. Corresponds to variant dbSNP:rs2128600Ensembl. | 1 | |
Natural variantiVAR_026684 | 196 | E → K in CMTDIC; partial loss of activity. 1 PublicationCorresponds to variant dbSNP:rs121908834EnsemblClinVar. | 1 | |
Natural variantiVAR_073292 | 274 | E → K Found in a patient with hereditary motor and sensory neuropathy; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs758897498Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40714 mRNA Translation: AAB39406.1 Frameshift. U89436 mRNA Translation: AAB88409.1 AK125213 mRNA Translation: BAG54166.1 AK223608 mRNA Translation: BAD97328.1 CH471059 Genomic DNA Translation: EAX07506.1 CH471059 Genomic DNA Translation: EAX07507.1 BC001933 mRNA Translation: AAH01933.1 BC004151 mRNA Translation: AAH04151.1 BC016689 mRNA Translation: AAH16689.1 |
CCDSi | CCDS368.1 |
RefSeqi | NP_003671.1, NM_003680.3 |
Genome annotation databases
Ensembli | ENST00000373477; ENSP00000362576; ENSG00000134684 |
GeneIDi | 8565 |
KEGGi | hsa:8565 |
MANE-Selecti | ENST00000373477.9; ENSP00000362576.4; NM_003680.4; NP_003671.1 |
UCSCi | uc001bvy.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40714 mRNA Translation: AAB39406.1 Frameshift. U89436 mRNA Translation: AAB88409.1 AK125213 mRNA Translation: BAG54166.1 AK223608 mRNA Translation: BAD97328.1 CH471059 Genomic DNA Translation: EAX07506.1 CH471059 Genomic DNA Translation: EAX07507.1 BC001933 mRNA Translation: AAH01933.1 BC004151 mRNA Translation: AAH04151.1 BC016689 mRNA Translation: AAH16689.1 |
CCDSi | CCDS368.1 |
RefSeqi | NP_003671.1, NM_003680.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1N3L | X-ray | 1.18 | A | 1-364 | [»] | |
1NTG | X-ray | 2.21 | A/B/C/D | 359-528 | [»] | |
1Q11 | X-ray | 1.60 | A | 1-364 | [»] | |
4Q93 | X-ray | 2.10 | A | 1-364 | [»] | |
4QBT | X-ray | 2.10 | A | 1-364 | [»] | |
5THH | X-ray | 1.96 | A | 4-342 | [»] | |
5THL | X-ray | 1.60 | A | 1-364 | [»] | |
SMRi | P54577 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114134, 87 interactors |
DIPi | DIP-50415N |
IntActi | P54577, 43 interactors |
MINTi | P54577 |
STRINGi | 9606.ENSP00000362576 |
Chemistry databases
BindingDBi | P54577 |
ChEMBLi | CHEMBL3179 |
DrugBanki | DB08617, 4-(2,2,2-TRIFLUOROETHYL)-L-PHENYLALANINE DB01766, Beta-(2-Naphthyl)-Alanine DB07205, N6-ISOPENTENYL-ADENOSINE-5'-MONOPHOSPHATE DB08371, p-Benzoyl-L-phenylalanine DB02709, Resveratrol DB03978, Tyrosinal DB00135, Tyrosine |
DrugCentrali | P54577 |
PTM databases
CarbonylDBi | P54577 |
GlyGeni | P54577, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | P54577 |
MetOSitei | P54577 |
PhosphoSitePlusi | P54577 |
SwissPalmi | P54577 |
Genetic variation databases
BioMutai | YARS |
DMDMi | 13638438 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00007074 |
Proteomic databases
CPTACi | CPTAC-299 CPTAC-300 |
EPDi | P54577 |
jPOSTi | P54577 |
MassIVEi | P54577 |
MaxQBi | P54577 |
PaxDbi | P54577 |
PeptideAtlasi | P54577 |
PRIDEi | P54577 |
ProteomicsDBi | 56686 |
Protocols and materials databases
ABCDi | P54577, 1 sequenced antibody |
Antibodypediai | 17005, 259 antibodies from 26 providers |
DNASUi | 8565 |
Genome annotation databases
Ensembli | ENST00000373477; ENSP00000362576; ENSG00000134684 |
GeneIDi | 8565 |
KEGGi | hsa:8565 |
MANE-Selecti | ENST00000373477.9; ENSP00000362576.4; NM_003680.4; NP_003671.1 |
UCSCi | uc001bvy.2, human |
Organism-specific databases
CTDi | 8565 |
DisGeNETi | 8565 |
GeneCardsi | YARS1 |
GeneReviewsi | YARS1 |
HGNCi | HGNC:12840, YARS1 |
HPAi | ENSG00000134684, Low tissue specificity |
MalaCardsi | YARS1 |
MIMi | 603623, gene 608323, phenotype |
neXtProti | NX_P54577 |
OpenTargetsi | ENSG00000134684 |
Orphaneti | 100045, Autosomal dominant intermediate Charcot-Marie-Tooth disease type C |
PharmGKBi | PA37431 |
VEuPathDBi | HostDB:ENSG00000134684 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2144, Eukaryota KOG2241, Eukaryota |
GeneTreei | ENSGT00940000156949 |
HOGENOMi | CLU_035267_3_0_1 |
InParanoidi | P54577 |
OMAi | YIGFEIS |
OrthoDBi | 852081at2759 |
PhylomeDBi | P54577 |
TreeFami | TF300898 |
Enzyme and pathway databases
BRENDAi | 6.1.1.1, 2681 |
PathwayCommonsi | P54577 |
Reactomei | R-HSA-379716, Cytosolic tRNA aminoacylation |
SignaLinki | P54577 |
Miscellaneous databases
BioGRID-ORCSi | 8565, 804 hits in 1025 CRISPR screens |
ChiTaRSi | YARS, human |
EvolutionaryTracei | P54577 |
GeneWikii | YARS |
GenomeRNAii | 8565 |
Pharosi | P54577, Tchem |
PROi | PR:P54577 |
RNActi | P54577, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134684, Expressed in frontal cortex and 238 other tissues |
ExpressionAtlasi | P54577, baseline and differential |
Genevisiblei | P54577, HS |
Family and domain databases
CDDi | cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002305, aa-tRNA-synth_Ic IPR012340, NA-bd_OB-fold IPR014729, Rossmann-like_a/b/a_fold IPR002547, tRNA-bd_dom IPR002307, Tyr-tRNA-ligase |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit PF01588, tRNA_bind, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS50886, TRBD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYYC_HUMAN | |
Accessioni | P54577Primary (citable) accession number: P54577 Secondary accession number(s): B3KWK4 Q53EN1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 216 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families