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Protein

NADPH dehydrogenase

Gene

namA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.1 Publication

Miscellaneous

Forms a charge transfer complex with a variety of phenolic compounds.

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.1 Publication

Cofactori

FMN1 Publication

Activity regulationi

Inhibited by p-hydroxybenzaldehyde (pHBA) and p-nitrophenol (pNP).

Kineticsi

The highest catalytic efficiency was observed with N-ethylmaleimide for which the KM is inferior to 1.0 µM.
  1. KM=19 µM for duroquinone1 Publication
  2. KM=109 µM for nitroglycerin1 Publication
  3. KM=293 µM for cyclohex-2-enone1 Publication
  4. KM=705 µM for 2,4,6-trinitrotoluene1 Publication
  5. KM=841 µM for menadione1 Publication
  6. KM=2602 µM for trans-hex-2-enal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei28Substrate1 Publication1
    Binding sitei60FMN1 Publication1
    Binding sitei102FMN1 Publication1
    Binding sitei215FMN1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi23 – 26FMN1 Publication4
    Nucleotide bindingi307 – 308FMN1 Publication2

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processDetoxification, Stress response
    LigandFlavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciBSUB:BSU23820-MONOMER
    SABIO-RKiP54550

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH dehydrogenase (EC:1.6.99.11 Publication)
    Alternative name(s):
    Xenobiotic reductase
    Gene namesi
    Name:namA
    Synonyms:yqjM
    Ordered Locus Names:BSU23820
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    • intracellular Source: GO_Central

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00002161192 – 338NADPH dehydrogenaseAdd BLAST337

    Proteomic databases

    PaxDbiP54550
    PRIDEiP54550

    Expressioni

    Inductioni

    By toxic xenobiotic compounds (2,4,6-trinitrotoluene and nitroglycerin), and in response to oxidative stress (hydrogen peroxide and paraquat).1 Publication

    Interactioni

    Subunit structurei

    Homotetramer. Composed of a dimer of active dimers.2 Publications

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100013071

    Structurei

    Secondary structure

    1338
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP54550
    SMRiP54550
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP54550

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni164 – 167Substrate binding1 Publication4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CCY Bacteria
    COG1902 LUCA
    HOGENOMiHOG000116232
    InParanoidiP54550
    KOiK00354
    OMAiYNPRWPW
    PhylomeDBiP54550

    Family and domain databases

    Gene3Di3.20.20.70, 1 hit
    HAMAPiMF_01614 NamA, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR023663 NADPH_DH
    IPR001155 OxRdtase_FMN_N
    PfamiView protein in Pfam
    PF00724 Oxidored_FMN, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P54550-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MARKLFTPIT IKDMTLKNRI VMSPMCMYSS HEKDGKLTPF HMAHYISRAI
    60 70 80 90 100
    GQVGLIIVEA SAVNPQGRIT DQDLGIWSDE HIEGFAKLTE QVKEQGSKIG
    110 120 130 140 150
    IQLAHAGRKA ELEGDIFAPS AIAFDEQSAT PVEMSAEKVK ETVQEFKQAA
    160 170 180 190 200
    ARAKEAGFDV IEIHAAHGYL IHEFLSPLSN HRTDEYGGSP ENRYRFLREI
    210 220 230 240 250
    IDEVKQVWDG PLFVRVSASD YTDKGLDIAD HIGFAKWMKE QGVDLIDCSS
    260 270 280 290 300
    GALVHADINV FPGYQVSFAE KIREQADMAT GAVGMITDGS MAEEILQNGR
    310 320 330
    ADLIFIGREL LRDPFFARTA AKQLNTEIPA PVQYERGW
    Length:338
    Mass (Da):37,584
    Last modified:January 23, 2007 - v3
    Checksum:iBDC52D34236326FE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D84432 Genomic DNA Translation: BAA12619.1
    AL009126 Genomic DNA Translation: CAB14314.1
    PIRiE69964
    RefSeqiNP_390263.1, NC_000964.3
    WP_003230377.1, NZ_JNCM01000036.1

    Genome annotation databases

    EnsemblBacteriaiCAB14314; CAB14314; BSU23820
    GeneIDi938698
    KEGGibsu:BSU23820
    PATRICifig|224308.179.peg.2595

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D84432 Genomic DNA Translation: BAA12619.1
    AL009126 Genomic DNA Translation: CAB14314.1
    PIRiE69964
    RefSeqiNP_390263.1, NC_000964.3
    WP_003230377.1, NZ_JNCM01000036.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1Z41X-ray1.30A/B1-338[»]
    1Z42X-ray1.85A/B1-338[»]
    1Z44X-ray1.40A/B1-338[»]
    1Z48X-ray1.80A/B1-338[»]
    ProteinModelPortaliP54550
    SMRiP54550
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100013071

    Proteomic databases

    PaxDbiP54550
    PRIDEiP54550

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB14314; CAB14314; BSU23820
    GeneIDi938698
    KEGGibsu:BSU23820
    PATRICifig|224308.179.peg.2595

    Phylogenomic databases

    eggNOGiENOG4105CCY Bacteria
    COG1902 LUCA
    HOGENOMiHOG000116232
    InParanoidiP54550
    KOiK00354
    OMAiYNPRWPW
    PhylomeDBiP54550

    Enzyme and pathway databases

    BioCyciBSUB:BSU23820-MONOMER
    SABIO-RKiP54550

    Miscellaneous databases

    EvolutionaryTraceiP54550

    Family and domain databases

    Gene3Di3.20.20.70, 1 hit
    HAMAPiMF_01614 NamA, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR023663 NADPH_DH
    IPR001155 OxRdtase_FMN_N
    PfamiView protein in Pfam
    PF00724 Oxidored_FMN, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNAMA_BACSU
    AccessioniPrimary (citable) accession number: P54550
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: November 7, 2018
    This is version 122 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
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