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UniProtKB - P54548 (RNZ_BACSU)
Protein
Ribonuclease Z
Gene
rnz
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.
UniRule annotation1 PublicationCatalytic activityi
- Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.UniRule annotation1 Publication EC:3.1.26.11
Cofactori
Zn2+2 PublicationsNote: Binds 2 Zn2+ ions.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 65 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Active sitei | 67 | Proton acceptorUniRule annotation1 Publication | 1 | |
Metal bindingi | 67 | Zinc 2; catalyticUniRule annotation2 Publications | 1 | |
Metal bindingi | 68 | Zinc 2; catalyticUniRule annotation2 Publications | 1 | |
Metal bindingi | 140 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 211 | Zinc 1; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 211 | Zinc 2; catalyticUniRule annotation2 Publications | 1 | |
Metal bindingi | 269 | Zinc 2; catalyticUniRule annotation2 Publications | 1 |
GO - Molecular functioni
- 3'-tRNA processing endoribonuclease activity Source: GO_Central
- identical protein binding Source: IntAct
- zinc ion binding Source: UniProtKB-UniRule
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU23840-MONOMER |
BRENDAi | 3.1.26.11, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease ZUniRule annotation (EC:3.1.26.11UniRule annotation1 Publication)Short name: RNase ZUniRule annotation Alternative name(s): tRNA 3 endonucleaseUniRule annotation tRNase ZUniRule annotation |
Gene namesi | Name:rnzUniRule annotation Synonyms:yqjK Ordered Locus Names:BSU23840 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000155849 | 1 – 307 | Ribonuclease ZAdd BLAST | 307 |
Proteomic databases
PaxDbi | P54548 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsBinary interactionsi
P54548
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-15572938,EBI-15572938 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-29070N |
STRINGi | 224308.BSU23840 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P54548 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54548 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RNase Z family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1234, Bacteria |
InParanoidi | P54548 |
OMAi | GTQRQMM |
PhylomeDBi | P54548 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 2 hits |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
i Sequence
Sequence statusi: Complete.
P54548-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELLFLGTGA GIPAKARNVT SVALKLLEER RSVWLFDCGE ATQHQILHTT
60 70 80 90 100
IKPRKIEKIF ITHMHGDHVY GLPGLLGSRS FQGGEDELTV YGPKGIKAFI
110 120 130 140 150
ETSLAVTKTH LTYPLAIQEI EEGIVFEDDQ FIVTAVSVIH GVEAFGYRVQ
160 170 180 190 200
EKDVPGSLKA DVLKEMNIPP GPVYQKIKKG ETVTLEDGRI INGNDFLEPP
210 220 230 240 250
KKGRSVVFSG DTRVSDKLKE LARDCDVLVH EATFAKEDRK LAYDYYHSTT
260 270 280 290 300
EQAAVTAKEA RAKQLILTHI SARYQGDASL ELQKEAVDVF PNSVAAYDFL
EVNVPRG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12617.1 AL009126 Genomic DNA Translation: CAB14316.1 |
PIRi | C69964 |
RefSeqi | NP_390265.1, NC_000964.3 WP_004398681.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14316; CAB14316; BSU_23840 |
GeneIDi | 938694 |
KEGGi | bsu:BSU23840 |
PATRICi | fig|224308.179.peg.2597 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12617.1 AL009126 Genomic DNA Translation: CAB14316.1 |
PIRi | C69964 |
RefSeqi | NP_390265.1, NC_000964.3 WP_004398681.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Y44 | X-ray | 2.10 | A/B | 1-307 | [»] | |
2FK6 | X-ray | 2.90 | A | 1-307 | [»] | |
4GCW | X-ray | 3.00 | A | 1-307 | [»] | |
SMRi | P54548 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-29070N |
STRINGi | 224308.BSU23840 |
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid |
Proteomic databases
PaxDbi | P54548 |
Genome annotation databases
EnsemblBacteriai | CAB14316; CAB14316; BSU_23840 |
GeneIDi | 938694 |
KEGGi | bsu:BSU23840 |
PATRICi | fig|224308.179.peg.2597 |
Phylogenomic databases
eggNOGi | COG1234, Bacteria |
InParanoidi | P54548 |
OMAi | GTQRQMM |
PhylomeDBi | P54548 |
Enzyme and pathway databases
BioCyci | BSUB:BSU23840-MONOMER |
BRENDAi | 3.1.26.11, 658 |
Miscellaneous databases
EvolutionaryTracei | P54548 |
Family and domain databases
CDDi | cd07717, RNaseZ_ZiPD-like_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01818, RNase_Z_BN, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro IPR013471, RNase_Z/BN |
Pfami | View protein in Pfam PF12706, Lactamase_B_2, 2 hits |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR02651, RNase_Z, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RNZ_BACSU | |
Accessioni | P54548Primary (citable) accession number: P54548 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | June 2, 2021 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families