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UniProtKB - P54493 (GLUP_BACSU)
Protein
Rhomboid protease GluP
Gene
gluP
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Important for normal cell division and sporulation. May act as a glucose exporter.
4 PublicationsCatalytic activityi
- Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains. EC:3.4.21.105
Activity regulationi
Inhibited by dichloroisocoumarin (DCI) and N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), but not by other serine protease inhibitors such as sulfonyl fluoride PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF).1 Publication
Temperature dependencei
Is more active at more active at 25 degrees Celsius than at 37 degrees Celsius.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 288 | NucleophileCurated | 1 | |
Active sitei | 339 | Charge relay systemCurated | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BioCyci | BSUB:BSU24870-MONOMER |
BRENDAi | 3.4.21.105, 658 |
Protein family/group databases
MEROPSi | S54.014 |
Names & Taxonomyi
Protein namesi | Recommended name: Rhomboid protease GluP (EC:3.4.21.105)Alternative name(s): Intramembrane serine protease |
Gene namesi | Name:gluP Synonyms:yqgP Ordered Locus Names:BSU24870 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 179 – 199 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 229 – 249 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 261 – 281 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 283 – 303 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 312 – 332 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 340 – 360 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 365 – 385 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 224 | R → A: Reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 237 | H → A: Reduced protease activity. 1 Publication | 1 | |
Mutagenesisi | 241 | N → A: No effect on protease activity in vitro. 1 Publication | 1 | |
Mutagenesisi | 286 | G → A: Loss of protease activity. 1 Publication | 1 | |
Mutagenesisi | 288 | S → A: Loss of protease activity. 1 Publication | 1 | |
Mutagenesisi | 339 | H → A: Loss of protease activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL4295579 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000049812 | 1 – 507 | Rhomboid protease GluPAdd BLAST | 507 |
Proteomic databases
PaxDbi | P54493 |
Interactioni
Protein-protein interaction databases
STRINGi | 224308.BSU24870 |
Chemistry databases
BindingDBi | P54493 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P54493 |
SMRi | P54493 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 424 – 457 | TPR 1Add BLAST | 34 | |
Repeati | 458 – 491 | TPR 2Add BLAST | 34 |
Sequence similaritiesi
Belongs to the peptidase S54 family.Curated
Keywords - Domaini
Repeat, TPR repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0457, Bacteria COG0705, Bacteria |
InParanoidi | P54493 |
OMAi | YPPVDDW |
PhylomeDBi | P54493 |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 2 hits |
SUPFAMi | SSF144091, SSF144091, 1 hit SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 2 hits PS50293, TPR_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
P54493-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFLLEYTYWK IAAHLVNSGY GVIQAGESDE IWLEAPDKSS HDLVRLYKHD
60 70 80 90 100
LDFRQEMVRD IEEQAERVER VRHQLGRRRM KLLNVFFSTE APVDDWEEIA
110 120 130 140 150
KKTFEKGTVS VEPAIVRGTM LRDDLQAVFP SFRTEDCSEE HASFENAQMA
160 170 180 190 200
RERFLSLVLK QEEQRKTEAA VFQNGKPTFT YLFIALQILM FFLLEINGGS
210 220 230 240 250
TNTETLVAFG AKENSLIAQG EWWRLLTPIV LHIGIAHLAF NTLALWSVGT
260 270 280 290 300
AVERMYGSGR FLLIYLAAGI TGSIASFVFS PYPSAGASGA IFGCLGALLY
310 320 330 340 350
VALSNRKMFL RTIGTNIIVI IIINLGFGFA VSNIDNSGHI GGLIGGFFAA
360 370 380 390 400
AALGLPKAGA FGKRLLSAVL LIALAVGFLY YGLHSPSHQE SALIQQASEL
410 420 430 440 450
YQEGKYEEVT ELLNGEAAQK DASADLLKIL AVSDIQIGEY DQAVSLLERA
460 470 480 490 500
VKKEPKDHAS YYNLALLYAE KNELAQAEKA IQTAVKLKPK EQRYKELQRQ
IENNKES
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 192 | F → S in BAA12519 (PubMed:8969508).Curated | 1 | |
Sequence conflicti | 370 | L → F in BAA12519 (PubMed:8969508).Curated | 1 | |
Sequence conflicti | 379 | L → S in BAA12519 (PubMed:8969508).Curated | 1 | |
Sequence conflicti | 499 | R → W in BAA12519 (PubMed:8969508).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12519.1 AL009126 Genomic DNA Translation: CAB14418.2 |
PIRi | B69957 |
RefSeqi | NP_390367.2, NC_000964.3 WP_003230128.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14418; CAB14418; BSU_24870 |
GeneIDi | 938211 |
KEGGi | bsu:BSU24870 |
PATRICi | fig|224308.179.peg.2706 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12519.1 AL009126 Genomic DNA Translation: CAB14418.2 |
PIRi | B69957 |
RefSeqi | NP_390367.2, NC_000964.3 WP_003230128.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6R0J | NMR | - | A | 1-176 | [»] | |
BMRBi | P54493 | |||||
SMRi | P54493 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU24870 |
Chemistry databases
BindingDBi | P54493 |
ChEMBLi | CHEMBL4295579 |
Protein family/group databases
MEROPSi | S54.014 |
Proteomic databases
PaxDbi | P54493 |
Genome annotation databases
EnsemblBacteriai | CAB14418; CAB14418; BSU_24870 |
GeneIDi | 938211 |
KEGGi | bsu:BSU24870 |
PATRICi | fig|224308.179.peg.2706 |
Phylogenomic databases
eggNOGi | COG0457, Bacteria COG0705, Bacteria |
InParanoidi | P54493 |
OMAi | YPPVDDW |
PhylomeDBi | P54493 |
Enzyme and pathway databases
BioCyci | BSUB:BSU24870-MONOMER |
BRENDAi | 3.4.21.105, 658 |
Family and domain databases
Gene3Di | 1.20.1540.10, 1 hit 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR022764, Peptidase_S54_rhomboid_dom IPR035952, Rhomboid-like_sf IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF01694, Rhomboid, 1 hit |
SMARTi | View protein in SMART SM00028, TPR, 2 hits |
SUPFAMi | SSF144091, SSF144091, 1 hit SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50005, TPR, 2 hits PS50293, TPR_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLUP_BACSU | |
Accessioni | P54493Primary (citable) accession number: P54493 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | July 7, 2009 | |
Last modified: | September 29, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families