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UniProtKB - P54488 (PBP2A_BACSU)
Protein
Penicillin-binding protein 2A
Gene
pbpA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Involved in the synthesis of peptidoglycan associated with cell wall elongation, especially following spore germination (PubMed:9139922).
Has a partially redundant function with PBP 1 (ponA) or PBP 4 (pbpD) during spore outgrowth (PubMed:9851991).
Plays a redundant role with PbpH in determining the rod shape of the cell during vegetative growth and spore outgrowth (PubMed:12896990).
3 PublicationsCatalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.Curated EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 397 | Acyl-ester intermediateBy similarity | 1 |
GO - Molecular functioni
- penicillin binding Source: GO_Central
- peptidoglycan L,D-transpeptidase activity Source: GO_Central
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC
GO - Biological processi
- cell wall organization Source: GO_Central
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- regulation of cell shape Source: UniProtKB-KW
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU25000-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | X52.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pbpA1 Publication Synonyms:yqgF Ordered Locus Names:BSU25000 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Other locations
- Forespore inner membrane 1 Publication; Single-pass membrane protein Curated
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 22 – 42 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype during vegetative growth rate in rich media, no difference in sporulation or initial spore germination. Spores have a significant delay in outgrowth, between 60-90 minutes cells swell rather than elongate, upon eventual elongation they have a larger diameter and are often bent (PubMed:9139922, PubMed:9851991). Single deletions have reduced peptidoglycan (PG) synthesis and turnover rates. Double ponA-pbpA deletions spores have greatly decreased viability, PG synthesis and elongate poorly; increased levels of Mg2+ increase spore viability. Double pbpA-pbpD deletions spores have greatly decreased spore outgrowth and peptidoglycan synthesis (PubMed:9851991). Single pbpA mutants are less resistant to ceftriaxone and mecillinam. Double pbpA-pbpH mutants cannot be made, suggesting the 2 proteins have redundant, essential roles in vegetative growth (PubMed:12896990).3 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195473 | 1 – 716 | Penicillin-binding protein 2AAdd BLAST | 716 |
Proteomic databases
PaxDbi | P54488 |
PRIDEi | P54488 |
Expressioni
Developmental stagei
Expressed in vegetative cells. Also present in dormant spores in the forespore inner membrane.1 Publication
Inductioni
Transcribed during vegetative phase, drops off during sporulation, rises again 30-40 minutes after germination. A small amount is present in dormant spores.1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 689 – 716 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 689 – 710 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the transpeptidase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0768, Bacteria |
OMAi | REIMDAY |
PhylomeDBi | P54488 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
i Sequence
Sequence statusi: Complete.
P54488-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRNKPKKQN HKEKKKSLPI RLNILFLAAF VIFTWIIVEL GIKQIVQGDD
60 70 80 90 100
YKNQANKQEQ SEVSSAVPRG KIYDRNFNAI VTNKALNAIT YTRSKSTTQE
110 120 130 140 150
QRLKIAKKLS DMIKVDTKKV TERDKKDYWI LTRPKEAKKL ISSKERQQVE
160 170 180 190 200
DKKISDDDLY QLQLKRITDK QLNELTDKDM QILAIKRQMD SGYALTPQYI
210 220 230 240 250
KNEDVSAKEM AVVSEHLDEL PGVDVTSDWE REYPYKNLLR SVLGSVSSSN
260 270 280 290 300
EGLPSNLLDH YLSLGYSRND RVGKSYLEYQ YESLLQGQKA KVENITDSKG
310 320 330 340 350
NVTGTKTVSE GKAGKDLVLT IDIDLQKSVE KIIEKKLKAA KARPSTELLD
360 370 380 390 400
RAFVVMMDPR NGEVLTMAGK QIKRENGAYK FDDYALGAMT SSYAMGSAVK
410 420 430 440 450
GATVLTGLQT GAINLNTVFK DEPLYIGQDK RGKKSWQNLG PVGIQTALEK
460 470 480 490 500
SSNVFMFKTA IAVGKGEYKP HQALPLDTSA FDTFRNYFSQ FGLGVKTGID
510 520 530 540 550
LPNEMTGYKG TSRLSGFLLD FAIGQYDTYT PLELAQYVST IANGGYRMKP
560 570 580 590 600
QLVKEVRDSN AKKGIGAVVD SVQPEVLNKV DMKSSYIEEV QAGFRRVATK
610 620 630 640 650
GTAAGQLASA SYKPAAKTGT AQSFYDGPDK SKTGTDTYNT TLVAYAPADN
660 670 680 690 700
PEIAISVVVP WTYIDYNQRY SITNEIGREV MDKYFELKSK QDKEGTQQKN
710
KDKIEENAEN TTSSDN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12509.1 AL009126 Genomic DNA Translation: CAB14430.1 |
PIRi | E69672 |
RefSeqi | NP_390379.1, NC_000964.3 WP_004398702.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14430; CAB14430; BSU_25000 |
GeneIDi | 938199 |
KEGGi | bsu:BSU25000 |
PATRICi | fig|224308.179.peg.2719 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84432 Genomic DNA Translation: BAA12509.1 AL009126 Genomic DNA Translation: CAB14430.1 |
PIRi | E69672 |
RefSeqi | NP_390379.1, NC_000964.3 WP_004398702.1, NZ_JNCM01000036.1 |
3D structure databases
AlphaFoldDBi | P54488 |
SMRi | P54488 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU25000 |
Protein family/group databases
MEROPSi | X52.001 |
Proteomic databases
PaxDbi | P54488 |
PRIDEi | P54488 |
Genome annotation databases
EnsemblBacteriai | CAB14430; CAB14430; BSU_25000 |
GeneIDi | 938199 |
KEGGi | bsu:BSU25000 |
PATRICi | fig|224308.179.peg.2719 |
Phylogenomic databases
eggNOGi | COG0768, Bacteria |
OMAi | REIMDAY |
PhylomeDBi | P54488 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | BSUB:BSU25000-MONOMER |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR005311, PBP_dimer IPR036138, PBP_dimer_sf IPR001460, PCN-bd_Tpept |
Pfami | View protein in Pfam PF03717, PBP_dimer, 1 hit PF00905, Transpeptidase, 1 hit |
SUPFAMi | SSF56519, SSF56519, 1 hit SSF56601, SSF56601, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PBP2A_BACSU | |
Accessioni | P54488Primary (citable) accession number: P54488 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | May 25, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families