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Entry version 137 (07 Apr 2021)
Sequence version 1 (01 Oct 1996)
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Protein

Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB

Gene

mtaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi11Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi47Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi78Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi153Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S-S-AdoMet)PROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processtRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU25430-MONOMER

BRENDA Comprehensive Enzyme Information System

More...
BRENDAi
2.8.4.5, 658

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB (EC:2.8.4.51 Publication)
Alternative name(s):
tRNA-t(6)A37 methylthiotransferase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mtaB
Synonyms:tmtB, yqeV
Ordered Locus Names:BSU25430
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001417291 – 451Threonylcarbamoyladenosine tRNA methylthiotransferase MtaBAdd BLAST451

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P54462

PRoteomics IDEntifications database

More...
PRIDEi
P54462

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU25430

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P54462

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini2 – 114MTTase N-terminalPROSITE-ProRule annotationAdd BLAST113
Domaini139 – 369Radical SAM corePROSITE-ProRule annotationAdd BLAST231
Domaini372 – 437TRAMPROSITE-ProRule annotationAdd BLAST66

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0621, Bacteria

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P54462

Identification of Orthologs from Complete Genome Data

More...
OMAi
HFHIPLQ

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P54462

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.12160, 1 hit
3.80.30.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006638, Elp3/MiaB/NifB
IPR005839, Methylthiotransferase
IPR020612, Methylthiotransferase_CS
IPR013848, Methylthiotransferase_N
IPR038135, Methylthiotransferase_N_sf
IPR006467, MiaB-like_C
IPR007197, rSAM
IPR023404, rSAM_horseshoe
IPR034557, ThrcA_tRNA_MEthiotransferase
IPR002792, TRAM_dom

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04055, Radical_SAM, 1 hit
PF01938, TRAM, 1 hit
PF00919, UPF0004, 1 hit

Structure-Function Linkage Database

More...
SFLDi
SFLDS00029, Radical_SAM, 1 hit
SFLDF00295, threonylcarbamoyladenosine_tRN, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00729, Elp3, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01579, MiaB-like-C, 1 hit
TIGR00089, TIGR00089, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51449, MTTASE_N, 1 hit
PS01278, MTTASE_RADICAL, 1 hit
PS51918, RADICAL_SAM, 1 hit
PS50926, TRAM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P54462-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATVAFHTLG CKVNHYETEA IWQLFKEAGY ERRDFEQTAD VYVINTCTVT
60 70 80 90 100
NTGDKKSRQV IRRAIRQNPD GVICVTGCYA QTSPAEIMAI PGVDIVVGTQ
110 120 130 140 150
DREKMLGYID QYREERQPIN GVSNIMKARV YEELDVPAFT DRTRASLKIQ
160 170 180 190 200
EGCNNFCTFC IIPWARGLLR SRDPEEVIKQ AQQLVDAGYK EIVLTGIHTG
210 220 230 240 250
GYGEDMKDYN FAKLLSELDT RVEGVKRIRI SSIEASQITD EVIEVLDRSD
260 270 280 290 300
KIVNHLHIPI QSGSNTVLKR MRRKYTMEFF ADRLNKLKKA LPGLAVTSDV
310 320 330 340 350
IVGFPGETEE EFMETYNFIK EHKFSELHVF PYSKRTGTPA ARMEDQVDEN
360 370 380 390 400
VKNERVHRLI ALSDQLAKEY ASQYENEVLE IIPEEAFKET EEENMFVGYT
410 420 430 440 450
DNYMKVVFKG TEDMIGKIVK VKILKAGYPY NEGQFVRVVE DEITEHMRLS

S
Length:451
Mass (Da):51,658
Last modified:October 1, 1996 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9FD45437F5CE3552
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12468.1
D83717 Genomic DNA Translation: BAA12080.1
AL009126 Genomic DNA Translation: CAB14485.1

Protein sequence database of the Protein Information Resource

More...
PIRi
E69952

NCBI Reference Sequences

More...
RefSeqi
NP_390421.1, NC_000964.3
WP_003230017.1, NZ_JNCM01000036.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB14485; CAB14485; BSU_25430

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
937857

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU25430

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.2764

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84432 Genomic DNA Translation: BAA12468.1
D83717 Genomic DNA Translation: BAA12080.1
AL009126 Genomic DNA Translation: CAB14485.1
PIRiE69952
RefSeqiNP_390421.1, NC_000964.3
WP_003230017.1, NZ_JNCM01000036.1

3D structure databases

SMRiP54462
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU25430

Proteomic databases

PaxDbiP54462
PRIDEiP54462

Genome annotation databases

EnsemblBacteriaiCAB14485; CAB14485; BSU_25430
GeneIDi937857
KEGGibsu:BSU25430
PATRICifig|224308.179.peg.2764

Phylogenomic databases

eggNOGiCOG0621, Bacteria
InParanoidiP54462
OMAiHFHIPLQ
PhylomeDBiP54462

Enzyme and pathway databases

BioCyciBSUB:BSU25430-MONOMER
BRENDAi2.8.4.5, 658

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
InterProiView protein in InterPro
IPR006638, Elp3/MiaB/NifB
IPR005839, Methylthiotransferase
IPR020612, Methylthiotransferase_CS
IPR013848, Methylthiotransferase_N
IPR038135, Methylthiotransferase_N_sf
IPR006467, MiaB-like_C
IPR007197, rSAM
IPR023404, rSAM_horseshoe
IPR034557, ThrcA_tRNA_MEthiotransferase
IPR002792, TRAM_dom
PfamiView protein in Pfam
PF04055, Radical_SAM, 1 hit
PF01938, TRAM, 1 hit
PF00919, UPF0004, 1 hit
SFLDiSFLDS00029, Radical_SAM, 1 hit
SFLDF00295, threonylcarbamoyladenosine_tRN, 1 hit
SMARTiView protein in SMART
SM00729, Elp3, 1 hit
TIGRFAMsiTIGR01579, MiaB-like-C, 1 hit
TIGR00089, TIGR00089, 1 hit
PROSITEiView protein in PROSITE
PS51449, MTTASE_N, 1 hit
PS01278, MTTASE_RADICAL, 1 hit
PS51918, RADICAL_SAM, 1 hit
PS50926, TRAM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMTAB_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P54462
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 7, 2021
This is version 137 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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