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UniProtKB - P54420 (ASNB_BACSU)
Protein
Asparagine synthetase [glutamine-hydrolyzing] 1
Gene
asnB
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Main asparagine synthetase in vegetative cells.
Catalytic activityi
- EC:6.3.5.4
: L-asparagine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route). This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | For GATase activityBy similarity | 1 | |
Binding sitei | 102 | GlutamineBy similarity | 1 | |
Binding sitei | 288 | ATP; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Sitei | 363 | Important for beta-aspartyl-AMP intermediate formationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 361 – 362 | ATPBy similarity | 2 |
GO - Molecular functioni
- asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- glutamine metabolic process Source: UniProtKB-KW
- L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Asparagine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | BSUB:BSU30540-MONOMER |
UniPathwayi | UPA00134;UER00195 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:asnB Synonyms:asn Ordered Locus Names:BSU30540 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056932 | 2 – 632 | Asparagine synthetase [glutamine-hydrolyzing] 1Add BLAST | 631 |
Proteomic databases
jPOSTi | P54420 |
PaxDbi | P54420 |
PRIDEi | P54420 |
Interactioni
Protein-protein interaction databases
IntActi | P54420, 3 interactors |
MINTi | P54420 |
STRINGi | 224308.BSU30540 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 214 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 213 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 52 – 56 | Glutamine bindingBy similarity | 5 | |
Regioni | 77 – 79 | Glutamine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the asparagine synthetase family.Curated
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | COG0367, Bacteria |
InParanoidi | P54420 |
OMAi | DIHTWMR |
PhylomeDBi | P54420 |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P54420-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGFVGVFNK HPLAQTADQE ELIKQMNQMI VHRGPDSDGY FHDEHVGFGF
60 70 80 90 100
RRLSIIDVEN GGQPLSYEDE TYWIIFNGEI YNYIELREEL EAKGYTFNTD
110 120 130 140 150
SDTEVLLATY RHYKEEAASK LRGMFAFLIW NKNDHVLYGA RDPFGIKPLY
160 170 180 190 200
YTTINDQVYF ASERKSLMVA QNDIEIDKEA LQQYMSFQFV PEPSTLDAHV
210 220 230 240 250
KKVEPGSQFT IRPDGDITFK TYFKANFKPV QTEEDKLVKE VRDAIYDSVN
260 270 280 290 300
VHMRSDVPVG SFLSGGIDSS FIVSVAKEFH PSLKTFSVGF EQQGFSEVDV
310 320 330 340 350
AKETAAALGI ENISKVISPE EYMNELPKIV WHFDDPLADP AAIPLYFVAK
360 370 380 390 400
EAKKHVTVAL SGEGADELFG GYNIYREPLS LKPFERIPSG LKKMLLHVAA
410 420 430 440 450
VMPEGMRGKS LLERGCTPLQ DRYIGNAKIF EESVKKQLLK HYNPNLSYRD
460 470 480 490 500
VTKTYFTESS SYSDINKMQY VDIHTWMRGD ILLKADKMTM ANSLELRVPF
510 520 530 540 550
LDKVVFDVAS KIPDELKTKN GTTKYLLRKA AEGIVPEHVL NRKKLGFPVP
560 570 580 590 600
IRHWLKNEMN EWVRNIIQES QTDAYIHKDY VLQLLEDHCA DKADNSRKIW
610 620 630
TVLIFMIWHS INIEKRYMPE ELSHQPKEVI FV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 79 – 81 | EIY → VNL in AAB17067 (PubMed:9384377).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00243.1 AL009126 Genomic DNA Translation: CAB15032.1 U52812 Genomic DNA Translation: AAB17067.1 |
PIRi | H69590 |
RefSeqi | NP_390932.1, NC_000964.3 WP_004398625.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB15032; CAB15032; BSU_30540 |
GeneIDi | 937236 |
KEGGi | bsu:BSU30540 |
PATRICi | fig|224308.179.peg.3312 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008220 Genomic DNA Translation: AAC00243.1 AL009126 Genomic DNA Translation: CAB15032.1 U52812 Genomic DNA Translation: AAB17067.1 |
PIRi | H69590 |
RefSeqi | NP_390932.1, NC_000964.3 WP_004398625.1, NZ_JNCM01000036.1 |
3D structure databases
SMRi | P54420 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P54420, 3 interactors |
MINTi | P54420 |
STRINGi | 224308.BSU30540 |
Proteomic databases
jPOSTi | P54420 |
PaxDbi | P54420 |
PRIDEi | P54420 |
Protocols and materials databases
DNASUi | 937236 |
Genome annotation databases
EnsemblBacteriai | CAB15032; CAB15032; BSU_30540 |
GeneIDi | 937236 |
KEGGi | bsu:BSU30540 |
PATRICi | fig|224308.179.peg.3312 |
Phylogenomic databases
eggNOGi | COG0367, Bacteria |
InParanoidi | P54420 |
OMAi | DIHTWMR |
PhylomeDBi | P54420 |
Enzyme and pathway databases
UniPathwayi | UPA00134;UER00195 |
BioCyci | BSUB:BSU30540-MONOMER |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N IPR014729, Rossmann-like_a/b/a_fold |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ASNB_BACSU | |
Accessioni | P54420Primary (citable) accession number: P54420 Secondary accession number(s): O34902 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | May 30, 2000 | |
Last modified: | February 23, 2022 | |
This is version 143 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families