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Entry version 134 (26 Feb 2020)
Sequence version 3 (28 Jul 2009)
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Protein

Phosphoenolpyruvate carboxykinase (ATP)

Gene

pckA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei56SubstrateUniRule annotation1
Binding sitei192SubstrateUniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi198ManganeseUniRule annotation1
Binding sitei198ATPUniRule annotation1
Binding sitei198SubstrateUniRule annotation1
Metal bindingi217Manganese; via tele nitrogenUniRule annotation1
Binding sitei217ATPUniRule annotation1
Metal bindingi254ManganeseUniRule annotation1
Binding sitei282ATPUniRule annotation1
Binding sitei319ATPUniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Binding sitei444ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi233 – 241ATPUniRule annotation9

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
BSUB:BSU30560-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00138

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase (ATP)UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:pckAUniRule annotation
Synonyms:ppc
Ordered Locus Names:BSU30560
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiBacillus subtilis (strain 168)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224308 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000001570 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002038101 – 527Phosphoenolpyruvate carboxykinase (ATP)Add BLAST527

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P54418

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P54418

PRoteomics IDEntifications database

More...
PRIDEi
P54418

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
224308.BSU30560

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P54418

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105DJ1 Bacteria
COG1866 LUCA

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P54418

KEGG Orthology (KO)

More...
KOi
K01610

Identification of Orthologs from Complete Genome Data

More...
OMAi
MRYAGEM

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P54418

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00484 PEPCK_ATP, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.449.10, 1 hit
3.90.228.20, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00453 PEPCK_ATP, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS

The PANTHER Classification System

More...
PANTHERi
PTHR30031 PTHR30031, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01293 PEPCK_ATP, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF006294 PEP_crbxkin, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF68923 SSF68923, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00224 pckA, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00532 PEPCK_ATP, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P54418-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNSVDLTADL QALLTCPNVR HNLSAAQLTE KVLSRNEGIL TSTGAVRATT
60 70 80 90 100
GAYTGRSPKD KFIVEEESTK NKIDWGPVNQ PISEEAFERL YTKVVSYLKE
110 120 130 140 150
RDELFVFEGF AGADEKYRLP ITVVNEFAWH NLFARQLFIR PEGNDKKTVE
160 170 180 190 200
QPFTILSAPH FKADPKTDGT HSETFIIVSF EKRTILIGGT EYAGEMKKSI
210 220 230 240 250
FSIMNFLLPE RDILSMHCSA NVGEKGDVAL FFGLSGTGKT TLSADADRKL
260 270 280 290 300
IGDDEHGWSD TGVFNIEGGC YAKCIHLSEE KEPQIFNAIR FGSVLENVVV
310 320 330 340 350
DEDTREANYD DSFYTENTRA AYPIHMINNI VTPSMAGHPS AIVFLTADAF
360 370 380 390 400
GVLPPISKLT KEQAMYHFLS GYTSKLAGTE RGVTSPETTF STCFGSPFLP
410 420 430 440 450
LPAHVYAEML GKKIDEHGAD VFLVNTGWTG GGYGTGERMK LSYTRAMVKA
460 470 480 490 500
AIEGKLEDAE MITDDIFGLH IPAHVPGVPD HILQPENTWT NKEEYKEKAV
510 520
YLANEFKENF KKFAHTDAIA QAGGPLV
Length:527
Mass (Da):58,272
Last modified:July 28, 2009 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iFE424C4F7BAB2D88
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti10L → S in AAB17065 (PubMed:19383706).Curated1
Sequence conflicti364A → V in AAC00377 (PubMed:9387221).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF008220 Genomic DNA Translation: AAC00377.1
AL009126 Genomic DNA Translation: CAB15034.2
U52812 Genomic DNA Translation: AAB17065.1

Protein sequence database of the Protein Information Resource

More...
PIRi
F69673

NCBI Reference Sequences

More...
RefSeqi
NP_390934.2, NC_000964.3
WP_003229100.1, NZ_JNCM01000036.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CAB15034; CAB15034; BSU30560

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
937235

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
bsu:BSU30560

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|224308.179.peg.3314

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA Translation: AAC00377.1
AL009126 Genomic DNA Translation: CAB15034.2
U52812 Genomic DNA Translation: AAB17065.1
PIRiF69673
RefSeqiNP_390934.2, NC_000964.3
WP_003229100.1, NZ_JNCM01000036.1

3D structure databases

SMRiP54418
ModBaseiSearch...

Protein-protein interaction databases

STRINGi224308.BSU30560

Proteomic databases

jPOSTiP54418
PaxDbiP54418
PRIDEiP54418

Genome annotation databases

EnsemblBacteriaiCAB15034; CAB15034; BSU30560
GeneIDi937235
KEGGibsu:BSU30560
PATRICifig|224308.179.peg.3314

Phylogenomic databases

eggNOGiENOG4105DJ1 Bacteria
COG1866 LUCA
InParanoidiP54418
KOiK01610
OMAiMRYAGEM
PhylomeDBiP54418

Enzyme and pathway databases

UniPathwayiUPA00138
BioCyciBSUB:BSU30560-MONOMER

Family and domain databases

CDDicd00484 PEPCK_ATP, 1 hit
Gene3Di3.40.449.10, 1 hit
3.90.228.20, 1 hit
HAMAPiMF_00453 PEPCK_ATP, 1 hit
InterProiView protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS
PANTHERiPTHR30031 PTHR30031, 1 hit
PfamiView protein in Pfam
PF01293 PEPCK_ATP, 1 hit
PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
TIGRFAMsiTIGR00224 pckA, 1 hit
PROSITEiView protein in PROSITE
PS00532 PEPCK_ATP, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPCKA_BACSU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P54418
Secondary accession number(s): O34304
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 28, 2009
Last modified: February 26, 2020
This is version 134 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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