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UniProtKB - P54352 (EAS_DROME)
Protein
Ethanolamine kinase
Gene
eas
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis.
1 PublicationCatalytic activityi
: phosphatidylethanolamine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.1 Publication This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- choline kinase activity Source: FlyBase
- ethanolamine kinase activity Source: FlyBase
GO - Biological processi
- associative learning Source: FlyBase
- asymmetric neuroblast division Source: FlyBase
- brain development Source: FlyBase
- habituation Source: FlyBase
- long-term memory Source: FlyBase
- mechanosensory behavior Source: FlyBase
- mushroom body development Source: FlyBase
- phosphatidylethanolamine biosynthetic process Source: GO_Central
- phosphatidylethanolamine metabolic process Source: FlyBase
- response to hypoxia Source: FlyBase
- response to mechanical stimulus Source: FlyBase
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-1483213, Synthesis of PE |
UniPathwayi | UPA00558;UER00741 |
Names & Taxonomyi
Protein namesi | Recommended name: Ethanolamine kinase (EC:2.7.1.821 Publication)Short name: EK Alternative name(s): Protein easily shocked |
Gene namesi | Name:eas ORF Names:CG3525 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000536, eas |
VEuPathDBi | VectorBase:FBgn0000536 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: FlyBase
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
'Bang sensitive' phenotype; induction of paralysis with electrical stimulation results in a brief seizure, followed by a failure of the muscles to respond to giant fiber stimulation. This is due to an excitability defect caused by altered membrane phospholipid composition.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206231 | 1 – 518 | Ethanolamine kinaseAdd BLAST | 518 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 190 | Phosphoserine2 Publications | 1 | |
Modified residuei | 194 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P54352 |
PRIDEi | P54352 |
PTM databases
iPTMneti | P54352 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 58926, 23 interactors |
IntActi | P54352, 2 interactors |
STRINGi | 7227.FBpp0073990 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 88 | DisorderedSequence analysisAdd BLAST | 88 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 71 | Polar residuesSequence analysisAdd BLAST | 71 | |
Compositional biasi | 72 – 88 | Basic and acidic residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the choline/ethanolamine kinase family.Curated
Phylogenomic databases
eggNOGi | KOG4720, Eukaryota |
GeneTreei | ENSGT00950000182939 |
InParanoidi | P54352 |
OMAi | WTVLQKW |
PhylomeDBi | P54352 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf |
SUPFAMi | SSF56112, SSF56112, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform E (identifier: P54352-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGTETKSNSY TGQISTSGGN PKVMKDSLSL VRQTVNQQTL SLSQSNQVQN
60 70 80 90 100
QLNSHSNSNS YPNPSGSENK NENEQNSRDI RAKPEDKSRK EAIVPFVPIF
110 120 130 140 150
VEEADVIQGA KELLKVIRPT WDLSHVEFKI RVVPQIEDRY PAQNAMVMMT
160 170 180 190 200
LYSFTDGITN KLVGCFHKEI SKLNDENGGS YLPIKTQGLS PVQSEDPVII
210 220 230 240 250
EKEDDDEFTD DRAADDGSPV QYSDNVVLVR IYGNKTDLLI DRKAETQNFL
260 270 280 290 300
LLHTYGLAPS LYATFKNGLV YEYVPGTTLN TDSVLCPEIW PLVARRMAEM
310 320 330 340 350
HRKVRKHGDS SATKPMPMIW KKTQSFLDLV PERFSDAEKH KRVKETFLPI
360 370 380 390 400
GRLREEFNKL YEYLEALDSP IVFSHNDLLL GNVIYTQSLN TVNFIDYEYA
410 420 430 440 450
DYNFQAFDIG NHFAEMCGVD EVDYSRYPKR EFQLQWLRVY LEEYLQRSNI
460 470 480 490 500
QNDEVELLYV QVNQFALASH IFWTVWSLLQ AEHSTIDFDY VGYAFLRYNE
510
YLARKVEFLS LTAAKNNK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2JFQ7 | X2JFQ7_DROME | Easily shocked, isoform J | eas ala, Dmel\CG3525, EP770, EtnK, PC80 | 171 | Annotation score: |
Sequence cautioni
The sequence ABE73230 differs from that shown. Reason: Frameshift.Curated
The sequence ACV91649 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 – 152 | YPAQN…MMTLY → VSGPKCDGDDDA in AAC37209 (PubMed:7923374).CuratedAdd BLAST | 13 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053946 | 2 – 30 | GTETK…DSLSL → STIRSKKN in isoform G. CuratedAdd BLAST | 29 | |
Alternative sequenceiVSP_001068 | 130 – 152 | Missing in isoform A. 2 PublicationsAdd BLAST | 23 | |
Alternative sequenceiVSP_053947 | 152 – 171 | YSFTD…HKEIS → VLPMASQTNWSDVFIRRSPN in isoform F and isoform G. CuratedAdd BLAST | 20 | |
Alternative sequenceiVSP_053948 | 172 – 518 | Missing in isoform F and isoform G. CuratedAdd BLAST | 347 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35603 mRNA Translation: AAC37209.1 L35604 mRNA Translation: AAC37210.1 AE014298 Genomic DNA Translation: AAF48574.1 AE014298 Genomic DNA Translation: AAF48575.2 AE014298 Genomic DNA Translation: AAN09387.1 AE014298 Genomic DNA Translation: AAO41661.2 AE014298 Genomic DNA Translation: AFH07419.1 AE014298 Genomic DNA Translation: AFH07420.1 AY118437 mRNA Translation: AAM48466.1 BT025059 mRNA Translation: ABE73230.1 Sequence problems. BT099812 mRNA Translation: ACV91649.1 Frameshift. |
PIRi | A54980 |
RefSeqi | NP_001245706.1, NM_001258777.1 [P54352-5] NP_001245707.1, NM_001258778.2 [P54352-4] NP_001285319.1, NM_001298390.1 [P54352-5] NP_523364.2, NM_078640.4 [P54352-2] NP_727941.2, NM_167489.2 [P54352-2] NP_727942.1, NM_167490.3 [P54352-2] NP_727943.1, NM_167491.2 [P54352-2] NP_788914.2, NM_176741.3 [P54352-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0074211; FBpp0073990; FBgn0000536 [P54352-2] FBtr0074212; FBpp0073991; FBgn0000536 [P54352-2] FBtr0074214; FBpp0073993; FBgn0000536 [P54352-2] FBtr0308617; FBpp0300841; FBgn0000536 [P54352-5] FBtr0308618; FBpp0300842; FBgn0000536 [P54352-4] FBtr0308619; FBpp0300843; FBgn0000536 [P54352-2] FBtr0343519; FBpp0310123; FBgn0000536 [P54352-2] FBtr0343520; FBpp0310124; FBgn0000536 [P54352-5] |
GeneIDi | 32585 |
KEGGi | dme:Dmel_CG3525 |
UCSCi | CG3525-RB, d. melanogaster CG3525-RD, d. melanogaster CG3525-RE, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L35603 mRNA Translation: AAC37209.1 L35604 mRNA Translation: AAC37210.1 AE014298 Genomic DNA Translation: AAF48574.1 AE014298 Genomic DNA Translation: AAF48575.2 AE014298 Genomic DNA Translation: AAN09387.1 AE014298 Genomic DNA Translation: AAO41661.2 AE014298 Genomic DNA Translation: AFH07419.1 AE014298 Genomic DNA Translation: AFH07420.1 AY118437 mRNA Translation: AAM48466.1 BT025059 mRNA Translation: ABE73230.1 Sequence problems. BT099812 mRNA Translation: ACV91649.1 Frameshift. |
PIRi | A54980 |
RefSeqi | NP_001245706.1, NM_001258777.1 [P54352-5] NP_001245707.1, NM_001258778.2 [P54352-4] NP_001285319.1, NM_001298390.1 [P54352-5] NP_523364.2, NM_078640.4 [P54352-2] NP_727941.2, NM_167489.2 [P54352-2] NP_727942.1, NM_167490.3 [P54352-2] NP_727943.1, NM_167491.2 [P54352-2] NP_788914.2, NM_176741.3 [P54352-2] |
3D structure databases
SMRi | P54352 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 58926, 23 interactors |
IntActi | P54352, 2 interactors |
STRINGi | 7227.FBpp0073990 |
PTM databases
iPTMneti | P54352 |
Proteomic databases
PaxDbi | P54352 |
PRIDEi | P54352 |
Protocols and materials databases
DNASUi | 32585 |
Genome annotation databases
EnsemblMetazoai | FBtr0074211; FBpp0073990; FBgn0000536 [P54352-2] FBtr0074212; FBpp0073991; FBgn0000536 [P54352-2] FBtr0074214; FBpp0073993; FBgn0000536 [P54352-2] FBtr0308617; FBpp0300841; FBgn0000536 [P54352-5] FBtr0308618; FBpp0300842; FBgn0000536 [P54352-4] FBtr0308619; FBpp0300843; FBgn0000536 [P54352-2] FBtr0343519; FBpp0310123; FBgn0000536 [P54352-2] FBtr0343520; FBpp0310124; FBgn0000536 [P54352-5] |
GeneIDi | 32585 |
KEGGi | dme:Dmel_CG3525 |
UCSCi | CG3525-RB, d. melanogaster CG3525-RD, d. melanogaster CG3525-RE, d. melanogaster |
Organism-specific databases
CTDi | 32585 |
FlyBasei | FBgn0000536, eas |
VEuPathDBi | VectorBase:FBgn0000536 |
Phylogenomic databases
eggNOGi | KOG4720, Eukaryota |
GeneTreei | ENSGT00950000182939 |
InParanoidi | P54352 |
OMAi | WTVLQKW |
PhylomeDBi | P54352 |
Enzyme and pathway databases
UniPathwayi | UPA00558;UER00741 |
Reactomei | R-DME-1483213, Synthesis of PE |
Miscellaneous databases
BioGRID-ORCSi | 32585, 0 hits in 3 CRISPR screens |
GenomeRNAii | 32585 |
PROi | PR:P54352 |
Gene expression databases
ExpressionAtlasi | P54352, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf |
SUPFAMi | SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EAS_DROME | |
Accessioni | P54352Primary (citable) accession number: P54352 Secondary accession number(s): C8VV66 Q9VXI7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | March 24, 2009 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families