UniProtKB - P54302 (LUXQ_VIBHA)
Protein
Autoinducer 2 sensor kinase/phosphatase LuxQ
Gene
luxQ
Organism
Vibrio harveyi (Beneckea harveyi)
Status
Functioni
At low cell density, in absence of AI-2 (autoinducer 2), LuxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is transferred to LuxU, and ultimately to LuxO. At high cell density, in the presence of AI-2, the kinase activity is inactivated, and the response regulator domain has a phosphatase activity.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: UniProtKB-KW
- phosphorelay sensor kinase activity Source: InterPro
GO - Biological processi
- signal transduction by protein phosphorylation Source: CACAO
Keywordsi
Molecular function | Hydrolase, Kinase, Protein phosphatase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.13.3, 6632 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:luxQ |
Organismi | Vibrio harveyi (Beneckea harveyi) |
Taxonomic identifieri | 669 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › |
Subcellular locationi
- Cell inner membrane Curated; Multi-pass membrane protein Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 280 – 300 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074783 | 1 – 859 | Autoinducer 2 sensor kinase/phosphatase LuxQAdd BLAST | 859 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 492 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 | |
Modified residuei | 785 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Binds the complex formed by AI-2 and LuxP.
Binary interactionsi
P54302
With | #Exp. | IntAct |
---|---|---|
luxP [P54300] | 5 | EBI-1101486,EBI-1101482 |
Protein-protein interaction databases
IntActi | P54302, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P54302 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P54302 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 489 – 711 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 223 | |
Domaini | 736 – 851 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 116 |
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd00082, HisKA, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.30.450.220, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR015387, LuxQ-periplasm IPR043056, LuxQ-periplasm_N IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF09308, LuxQ-periplasm, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF47384, SSF47384, 1 hit SSF52172, SSF52172, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P54302-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTTRSNIKK RRSLATLITK IIILVLAPII LGIFIQSYYF SKQIIWQEVD
60 70 80 90 100
RTKQQTSALI HNIFDSHFAA IQIHHDSNSK SEVIRDFYTD RDTDVLNFFF
110 120 130 140 150
LSIDQSDPSH TPEFRFLTDH KGIIWDDGNA HFYGVNDLIL DSLANRVSFS
160 170 180 190 200
NNWYYINVMT SIGSRHMLVR RVPILDPSTG EVLGFSFNAV VLDNNFALME
210 220 230 240 250
KLKSESNVDN VVLVANSVPL ANSLIGDEPY NVADVLQRKS SDKRLDKLLV
260 270 280 290 300
IETPIVVNAV TTELCLLTVQ DNQSVVTLQI QHILAMLASI IGMIMIALMS
310 320 330 340 350
REWIESKVSA QLESLMSYTR SAREEKGFER FGGSDIEEFD HIGSTLESTF
360 370 380 390 400
EELEAQKKSF RDLFNFALSP IMVWSEESVL IQMNPAARKE LVIEDDHEIM
410 420 430 440 450
HPVFQGFKEK LTPHLKMAAQ GATLTGVNVP IGNKIYRWNL SPIRVDGDIS
460 470 480 490 500
GIIVQGQDIT TLIEAEKQSN IARREAEKSA QARADFLAKM SHEIRTPING
510 520 530 540 550
ILGVAQLLKD SVDTQEQKNQ IDVLCHSGEH LLAVLNDILD FSKIEQGKFN
560 570 580 590 600
IQKHPFSFTD TMRTLENIYR PICTNKGVEL VIENELDPNV EIFTDQVRLN
610 620 630 640 650
QILFNLVSNA VKFTPIGSIR LHAELEQFYG AENSVLVVEL TDTGIGIESD
660 670 680 690 700
KLDQMFEPFV QEESTTTREY GGSGLGLTIV KNLVDMLEGD VQVRSSKGGG
710 720 730 740 750
TTFVITLPVK DRERVLRPLE VSQRIKPEAL FDESLKVLLV EDNHTNAFIL
760 770 780 790 800
QAFCKKYKMQ VDWAKDGLDA MELLSDTTYD LILMDNQLPH LGGIETTHEI
810 820 830 840 850
RQNLRLGTPI YACTADTAKE TSDAFMAAGA NYVMLKPIKE NALHEAFVDF
KQRFLVERT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07069 Genomic DNA Translation: AAA20838.1 |
PIRi | S49046 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U07069 Genomic DNA Translation: AAA20838.1 |
PIRi | S49046 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZHH | X-ray | 1.94 | B | 39-278 | [»] | |
2HJ9 | X-ray | 2.34 | C/D | 53-271 | [»] | |
2HJE | X-ray | 1.70 | A | 53-271 | [»] | |
SMRi | P54302 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P54302, 1 interactor |
Enzyme and pathway databases
BRENDAi | 2.7.13.3, 6632 |
Miscellaneous databases
EvolutionaryTracei | P54302 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00156, REC, 1 hit |
Gene3Di | 3.30.450.220, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR015387, LuxQ-periplasm IPR043056, LuxQ-periplasm_N IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF09308, LuxQ-periplasm, 1 hit PF00072, Response_reg, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF47384, SSF47384, 1 hit SSF52172, SSF52172, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LUXQ_VIBHA | |
Accessioni | P54302Primary (citable) accession number: P54302 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1996 |
Last sequence update: | October 1, 1996 | |
Last modified: | August 12, 2020 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references